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1. 发明申请

WO0104273A3 GLUCOAMYLASE VARIANT 审中-公开
公开(公告)号：WO0104273A3
公开(公告)日：2001-09-07
申请号：PCT/DK0000373
申请日：2000-07-07
申请人： NOVOZYMES AS
发明人： NIELSEN BJARNE ROENFELDT , SVENDSEN ALLAN , PEDERSEN HENRIK , VIND JESPER , HENDRIKSEN HANNE VANG , FRANDSEN TORBEN PETER
IPC分类号： C12N15/09 , C12N1/15 , C12N9/34 , C12N9/96 , C12P19/02 , C12P19/20 , C12R1/685
CPC分类号： C12N9/2428 , C12P19/20 , Y02E50/17
摘要： The invention relates to a variant of a parent fungal glucoamylase, which exhibits altered properties, in particular improved thermal stability and/or increased specific activity.
摘要翻译：本发明涉及亲本真菌葡糖淀粉酶的变体，其具有改变的性质，特别是改进的热稳定性和/或增加的比活性。

2. 发明申请

WO0206442A2 CELL-WALL DEGRADING ENZYME VARIANTS 审中-公开
标题翻译：细胞壁降解酶变异
公开(公告)号：WO0206442A2
公开(公告)日：2002-01-24
申请号：PCT/DK0100505
申请日：2001-07-17
申请人： NOVOZYMES AS , GLAD SANNE O SCHROEDER , ANDERSEN CARSTEN , FRANDSEN TORBEN PETER
发明人： SCHUELEIN MARTIN DI , GLAD SANNE O SCHROEDER , ANDERSEN CARSTEN , FRANDSEN TORBEN PETER
IPC分类号： C12N9/88 , C12N
CPC分类号： C12N9/88
摘要： A variant of a cell-wall degrading enzyme having a beta-helix structure, which variant holds at least one substituent in a position determined by identifying all residues potentially belonging to a stack; characterising the stack as interior or exterior; characterising the stack as polar, hydrophobic or aromatic/heteroaromatic based on the dominating characteristics of the parent or wild-type enzyme stack residues and/or its orientation relative to the beta-helix (interior or exterior); optimising all stack positions of a stack either to hydrophobic aliphatic amino acids, hydrophobic aromatic or polar amino acids by allowing mutations within one or all positions to amino acids belonging to one of these groups; measuring thermostability of the variants by DSC or an application-related assay such as a Pad-Steam application test; and selecting the stabilized variants. Variant of a wild-type parent pectate lyase (EC 4.2.2.2) having the conserved amino acid residues D111, D141 or E141, D145, K165, R194 and R199 when aligned with the pectate lyase comprising the amino acid sequence of SEQ ID NO: 2 are preferred.
摘要翻译：具有β-螺旋结构的细胞壁降解酶的变体，该变体保持在通过鉴定潜在属于堆叠的所有残基而确定的位置中的至少一个取代基; 将堆栈表征为内部或外部; 基于母体或野生型酶堆残基的主导特性和/或其相对于β-螺旋（内部或外部）的取向，将所述叠层表征为极性，疏水性或芳族/杂芳族; 通过在属于这些基团之一的氨基酸的一个或所有位置内允许突变将堆叠的所有堆叠位置优化为疏水性脂族氨基酸，疏水性芳族或极性氨基酸; 通过DSC测量变体的热稳定性或应用相关测定，例如Pad-Steam应用测试; 并选择稳定的变体。当与包含SEQ ID NO：1的氨基酸序列的果胶酸裂解酶比对时，具有保守氨基酸残基D111，D141或E141，D145，K165，R194和R199的野生型亲本果胶酸裂合酶（EC 4.2.2.2） 2是优选的。

3. 发明专利

DK2305799T3 Maltogene alfa-amylasevarianter 未知
公开(公告)号：DK2305799T3
公开(公告)日：2016-07-25
申请号：DK10181883
申请日：1999-02-26
申请人： NOVOZYMES AS
发明人： CHERRY JOEL ROBERT , SVENDSEN ALLAN , ANDERSEN CARSTEN , BEIER LARS , FRANDSEN TORBEN PETER
IPC分类号： C12N9/28 , A21D8/04 , C12N1/15 , C12N1/21 , C12N15/09 , C12N15/55 , C12N15/56 , C12R1/07

4. 发明专利

AU2512999A Maltogenic alpha-amylase variants 未知
公开(公告)号：AU2512999A
公开(公告)日：1999-09-15
申请号：AU2512999
申请日：1999-02-26
申请人： NOVOZYMES AS
发明人： CHERRY JOEL ROBERT , SVENDSEN ALLAN , ANDERSEN CARSTEN , BEIER LARS , FRANDSEN TORBEN PETER
IPC分类号： C12N9/28 , A21D8/04 , C12N1/15 , C12N1/21 , C12N15/09 , C12N15/56 , C12R1/07
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.

5. 发明专利

DK1058724T3 Maltogene alpha-amylase varianter 未知
公开(公告)号：DK1058724T3
公开(公告)日：2011-03-21
申请号：DK99904736
申请日：1999-02-26
申请人： NOVOZYMES AS
发明人： ANDERSEN CARSTEN , CHERRY JOEL ROBERT , SVENDSEN ALLAN , BEIER LARS , FRANDSEN TORBEN PETER
IPC分类号： C12N9/28 , A21D8/04 , C12N1/15 , C12N1/21 , C12N15/09 , C12N15/56 , C12R1/07
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.

6. 发明专利

DE60137510D1 未知
公开(公告)号：DE60137510D1
公开(公告)日：2009-03-12
申请号：DE60137510
申请日：2001-07-17
申请人： NOVOZYMES AS
发明人： SCHUELEIN MARTIN , GLAD SANNE O SCHROEDER , ANDERSEN CARSTEN , FRANDSEN TORBEN PETER
IPC分类号： C12N9/88 , C11D3/386 , C12S3/04 , C12S11/00

7. 发明专利

AU783277B2 Lysophospholipase from aspergillus 未知
公开(公告)号：AU783277B2
公开(公告)日：2005-10-06
申请号：AU7772700
申请日：2000-10-12
申请人： NOVOZYMES AS
发明人： UDAGAWA HIROAKI , FRANDSEN TORBEN PETER , NIELSEN TOM ANTON BUSK , KAUPPINEN MARKUS SAKARI , CHRISTENSEN SOREN
IPC分类号： C12N9/18 , C12N9/16 , C12N15/63
摘要： The inventors have isolated lysophospholipases from Aspergillus (A. niger and A. oryzae) having molecular masses of about 68 kDa and amino acid sequences of 600-604 amino acid residues. The novel lysophospholipases have only a limited homology to known amino acid sequences. The inventors also isolated genes encoding the novel enzymes and cloned them into E. coli strains.

8. 发明专利

NZ505820A Enzyme variants based on the 3D structure of maltogenic alpha-amylase that have an altered pH optimum, thermostability, specific activity, cleavage pattern and ability to reduce the staling of bread 未知
公开(公告)号：NZ505820A
公开(公告)日：2002-10-25
申请号：NZ50582099
申请日：1999-02-26
申请人： NOVOZYMES AS
发明人： ANDERSEN CARSTEN , BEIER LARS , FRANDSEN TORBEN PETER , CHERRY JOEL ROBERT , SVENDSEN ALLAN
IPC分类号： A21D8/04 , C12N1/15 , C12N9/28 , C12N1/21 , C12N15/09 , C12N15/56 , C12R1/07
摘要： Described is the modifying of the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, for example an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.

9. 发明专利

DE60045487D1 未知
公开(公告)号：DE60045487D1
公开(公告)日：2011-02-17
申请号：DE60045487
申请日：2000-10-12
申请人： NOVOZYMES AS
发明人： UDAGAWA HIROAKI , FRANDSEN TORBEN PETER , NIELSEN TOM , KAUPPINEN MARKUS SAKARI , CHRISTENSEN S REN
IPC分类号： C12N9/16 , C12N9/18 , C12N15/63

10. 发明专利

AT421579T 未知
公开(公告)号：AT421579T
公开(公告)日：2009-02-15
申请号：AT01960180
申请日：2001-07-17
申请人： NOVOZYMES AS
发明人： SCHUELEIN MARTIN , GLAD SANNE O , ANDERSEN CARSTEN , FRANDSEN TORBEN PETER
IPC分类号： C12N9/88 , C11D3/386 , C12S3/04 , C12S11/00

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