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1. 发明申请

WO2020123845A1 METHODS FOR INCREASING THE PRODUCTIVITY OF A FILAMENTOUS FUNGAL CELL IN THE PRODUCTION OF A POLYPEPTIDE 审中-公开
公开(公告)号：WO2020123845A1
公开(公告)日：2020-06-18
申请号：PCT/US2019/066046
申请日：2019-12-12
申请人： NOVOZYMES A/S , MAIYURAN, Suchindra
发明人： MAIYURAN, Suchindra , LOPEZ DE LEON, Alfredo , SHASKY, Jeffrey
IPC分类号： C12N15/80 , C07K14/37
摘要： The present invention relates to a mutant of a parent filamentous fungal cell, comprising a coding sequence of a polypeptide of interest under the transcriptional control of a promoter regulated by a transcription factor, wherein the gene encoding the transcription factor is modified in the parent filamentous fungal cell to produce the mutant rendering the mutant deficient in the production of the transcription factor, which increases the productivity of the mutant in the production of the polypeptide of interest, and/or reduces or eliminates the cellulase-negative phenotype in the resulting mutant. The present invention also relates to a method for constructing such a mutant and a method of producing a polypeptide of interest with such a mutant.

2. 发明申请

WO2013028928A1 CELLULOLYTIC ENZYME COMPOSITIONS AND USES THEREOF 审中-公开
标题翻译：纤维素酶组合物及其用途
公开(公告)号：WO2013028928A1
公开(公告)日：2013-02-28
申请号：PCT/US2012/052163
申请日：2012-08-23
申请人： NOVOZYMES, INC. , SHASKY, Jeffrey , MAIYURAN, Suchindra , FISCHER, Amanda
发明人： SHASKY, Jeffrey , MAIYURAN, Suchindra , FISCHER, Amanda
IPC分类号： C12N1/22 , C12P19/02 , C12N9/24
CPC分类号： C12P19/14 , C07K14/38 , C12N9/2437 , C12N9/2445 , C12P19/02 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01037 , C12Y302/01091 , C12Y302/01176 , Y02E50/16 , Y02P20/52
摘要： The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.
摘要翻译：本发明涉及产生纤维素分解酶组合物的重组丝状真菌宿主细胞和生产和使用该组合物的方法。

3. 发明申请

WO2011075677A2 METHODS FOR PRODUCING POLYPEPTIDES IN PROTEASE-DEFICIENT MUTANTS OF TRICHODERMA 审中-公开
标题翻译：用于在TRICHODERMA的蛋白酶缺陷型突变体中生产多肽的方法
公开(公告)号：WO2011075677A2
公开(公告)日：2011-06-23
申请号：PCT/US2010/061105
申请日：2010-12-17
申请人： NOVOZYMES, INC. , MAIYURAN, Suchindra , JANG, Abigall , BROWN, Kimberly , MERINO, Sandra , SHASKY, Jeffrey , ABBATE, Eric
发明人： MAIYURAN, Suchindra , JANG, Abigall , BROWN, Kimberly , MERINO, Sandra , SHASKY, Jeffrey , ABBATE, Eric
IPC分类号： C12N1/14 , C12N9/58 , C12N15/57 , C12N15/80 , C12P21/02
CPC分类号： C12P21/00 , C12N9/58 , C12N15/80 , C12P21/02
摘要： The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.
摘要翻译：本发明涉及亲本木霉属菌株的突变体，其包含编码多肽的多核苷酸和选自以下的一种或多种（几种）基因：第一枯草杆菌蛋白酶 - 丝氨酸蛋白酶基因，第一天冬氨酸蛋白酶基因，胰蛋白酶样丝氨酸蛋白酶基因，第二枯草杆菌蛋白酶样丝氨酸蛋白酶基因和第二天冬氨酸蛋白酶基因，其中所述一个或多个（几个）基因被修饰使得所述突变体选自第一枯草杆菌蛋白酶样丝氨酸蛋白酶，第一天冬氨酸蛋白酶，胰蛋白酶样丝氨酸蛋白酶，第二枯草杆菌蛋白酶样丝氨酸蛋白酶和第二枯草杆菌蛋白酶样丝氨酸蛋白酶的一种或多种（几种）酶产生缺陷的菌株当在相同条件下培养时，与亲本木霉属菌株相比，它们分别与天冬氨酸蛋白酶相比。本发明还涉及在这种突变体中生产多肽的方法和生产这种突变体的方法。

4. 发明申请

WO2019046703A1 METHODS FOR IMPROVING GENOME EDITING IN FUNGI 审中-公开
公开(公告)号：WO2019046703A1
公开(公告)日：2019-03-07
申请号：PCT/US2018/049066
申请日：2018-08-31
申请人： NOVOZYMES A/S , SHASKY, Jeffrey
发明人： SHASKY, Jeffrey , MAIYURAN, Suchindra , LEHMBECK, Jan
IPC分类号： C12N15/01 , C12N15/63 , C12N15/80 , C12N15/90
CPC分类号： C12N15/80 , C12N15/01 , C12N15/102 , C12N15/63 , C12N15/902
摘要： The present disclosure relates to methods for modifying the genome of a fungal cell, comprising: (A) introducing into the fungal cell: (i) a first nucleic acid construct comprising a polynucleotide encoding an RNA-guided DNA endonuclease; (ii) a second nucleic acid construct comprising (a) a U6 promoter sequence operably linked to (1) a transfer RNA encoding sequence, (2) a single guide RNA encoding sequence, and (b) a U6 terminator sequence; and optionally (iii) a third nucleic acid construct comprising a donor DNA for modifying the genome; and (B) selecting a transformant of the fungal cell, wherein the genome is modified.

5. 发明申请

WO2016145084A1 METHODS OF INTRODUCING MULTIPLE EXPRESSION CONSTRUCTS INTO A EUKARYOTIC CELL 审中-公开
标题翻译：将多种表达式构建体引入原核细胞的方法
公开(公告)号：WO2016145084A1
公开(公告)日：2016-09-15
申请号：PCT/US2016/021568
申请日：2016-03-09
申请人： NOVOZYMES A/S , JIN, Qiming
发明人： JIN, Qiming , SHASKY, Jeffrey , MOYER, Donna , JANG, Abigail , MUZZI-ERICHSEN, Gloria , KLEINDIENST, Cara
IPC分类号： C12N15/90 , C12N15/79
CPC分类号： C12N15/79 , C12N15/902 , C12N2800/30
摘要： The present invention relates to methods of introducing multiple expression constructs into a eukaryotic cell, methods of constructing a eukaryotic cell having multiple target loci for expressing multiple heterologous proteins of interest, eukaryotic cells for expressing multiple heterologous proteins of interest, and methods of production of multiple heterologous proteins of interest.
摘要翻译：本发明涉及将多种表达构建体引入真核细胞的方法，构建具有多个目的基因座的表达多种感兴趣的异源蛋白质的真核细胞的方法，用于表达感兴趣的多种异源蛋白质的真核细胞，以及生产多种异源蛋白质的方法感兴趣的异源蛋白质。

6. 发明申请

WO2015187935A1 ENZYME COMPOSITIONS AND USES THEREOF 审中-公开
标题翻译：酶组成及其用途
公开(公告)号：WO2015187935A1
公开(公告)日：2015-12-10
申请号：PCT/US2015/034179
申请日：2015-06-04
申请人： NOVOZYMES A/S , SHASKY, Jeffrey , YAVER, Debbie , MOYER, Donna
发明人： SHASKY, Jeffrey , YAVER, Debbie , MOYER, Donna
IPC分类号： C12P19/02 , C12N9/24
CPC分类号： C12P19/14 , C12N9/0004 , C12N9/0065 , C12N9/24 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12P19/02 , C12P2201/00 , C12P2203/00 , C12Y111/01006 , C12Y302/01021 , C12Y302/01037 , C12Y302/01091 , C12Y302/01176
摘要： The present invention relates to enzyme compositions and processes of producing and using the compositions for the saccharification of lignocellulosic material.
摘要翻译：本发明涉及酶组合物和生产和使用用于木质纤维素材料糖化的组合物的方法。

7. 发明公开

EP3268482A1 METHODS OF INTRODUCING MULTIPLE EXPRESSION CONSTRUCTS INTO A EUKARYOTIC CELL 有权
标题翻译：引入多种表达构建体进入真核细胞的方法
公开(公告)号：EP3268482A1
公开(公告)日：2018-01-17
申请号：EP16711089.9
申请日：2016-03-09
申请人： Novozymes A/S
发明人： JIN, Qiming , SHASKY, Jeffrey , MOYER, Donna , JANG, Abigail , MUZZI-ERICHSEN, Gloria , KLEINDIENST, Cara
IPC分类号： C12N15/90 , C12N15/79
摘要： The present invention relates to methods of introducing multiple expression constructs into a eukaryotic cell, methods of constructing a eukaryotic cell having multiple target loci for expressing multiple heterologous proteins of interest, eukaryotic cells for expressing multiple heterologous proteins of interest, and methods of production of multiple heterologous proteins of interest.

8. 发明公开

EP3152315A1 ENZYME COMPOSITIONS AND USES THEREOF 有权
标题翻译： ENZYMZUSAMMENSETZUNGEN UND VERWENDUNGEN DAVON
公开(公告)号：EP3152315A1
公开(公告)日：2017-04-12
申请号：EP15730352.0
申请日：2015-06-04
申请人： Novozymes A/S
发明人： YAVER, Debbie , MOYER, Donna , SHASKY, Jeffrey
IPC分类号： C12P19/02 , C12N9/24
CPC分类号： C12P19/14 , C12N9/0004 , C12N9/0065 , C12N9/24 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12P19/02 , C12P2201/00 , C12P2203/00 , C12Y111/01006 , C12Y302/01021 , C12Y302/01037 , C12Y302/01091 , C12Y302/01176
摘要： The present invention relates to enzyme compositions and processes of producing and using the compositions for the saccharification of lignocellulosic material.
摘要翻译：本发明涉及酶组合物和生产和使用用于木质纤维素材料糖化的组合物的方法。

9. 发明公开

EP3894565A1 METHODS FOR INCREASING THE PRODUCTIVITY OF A FILAMENTOUS FUNGAL CELL IN THE PRODUCTION OF A POLYPEPTIDE 审中-实审
公开(公告)号：EP3894565A1
公开(公告)日：2021-10-20
申请号：EP19836744.3
申请日：2019-12-12
申请人： Novozymes A/S
发明人： LOPEZ DE LEON, Alfredo , SHASKY, Jeffrey , MAIYURAN, Suchindra
IPC分类号： C12N15/80 , C07K14/37

10. 发明授权

EP3268482B1 METHODS OF INTRODUCING MULTIPLE EXPRESSION CONSTRUCTS INTO A EUKARYOTIC CELL 有权
公开(公告)号：EP3268482B1
公开(公告)日：2019-12-11
申请号：EP16711089.9
申请日：2016-03-09
申请人： Novozymes A/S
发明人： JIN, Qiming , SHASKY, Jeffrey , MOYER, Donna , JANG, Abigail , MUZZI-ERICHSEN, Gloria , KLEINDIENST, Cara
IPC分类号： C12N15/90 , C12N15/79

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