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1. 发明申请

WO1995031533A1 AN ENZYME WITH ENDO-1,3(4)-'beta'-GLUCANASE ACTIVITY 审中-公开
标题翻译：具有内切-1,3（4）-β-GLUCANASE活性的酶
公开(公告)号：WO1995031533A1
公开(公告)日：1995-11-23
申请号：PCT/DK1995000188
申请日：1995-05-11
申请人： NOVO NORDISK A/S , KOFOD, Lene, Venke , ANDERSEN, Lene, Nonboe , KAUPPINEN, Markus, Sakari , CHRISTGAU, Stephan , DALBOGE, Henrik , OLSEN, Hans, Sejr , BREINHOLT, Jens
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： C12Y302/01006 , A21D8/042 , A61K8/66 , A61Q11/00 , C11D3/38645 , C12N9/244 , D06M16/003
摘要： The present invention relates to an enzyme with endo-beta-glucanase activity, encoded by a DNA sequence. Further, the invention relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, the use of said enzyme or said preparation for a number of uses including the degradation or modification of beta-glucan containing materials. Also contemplated is an isolated substantially pure culture of Saccharomyces cerevisiae NN049006, transformed with a DNA sequence derived from Acremonium sp.
摘要翻译：本发明涉及由DNA序列编码的内切β-葡聚糖酶活性的酶。此外，本发明涉及编码该酶的DNA构建体，该酶的制备方法，含该酶的酶制剂，所述酶或所述制剂的用途，包括降解或修饰含有β-葡聚糖的多种用途材料。还考虑了用源自Acremonium sp。的DNA序列转化的酿酒酵母NN049006的分离的基本上纯的培养物。

2. 发明申请

WO1994021786A1 AN ENZYME WITH PECTIN LYASE ACTIVITY 审中-公开
标题翻译：具有皮质激素活性的酶
公开(公告)号：WO1994021786A1
公开(公告)日：1994-09-29
申请号：PCT/DK1994000105
申请日：1994-03-11
申请人： NOVO NORDISK A/S , DALBOGE, Henrik , KOFOD, Lene, Venke , KAUPPINEN, Markus, Sakari , ANDERSEN, Lene, Nonboe , CHRISTGAU, Stephan , HELDT-HANSEN, Hans, Peter
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/88
CPC分类号： C12N9/88 , A61B42/40 , A61B42/50
摘要： An enzyme exhibiting pectin lyase activity, which enzyme is immunologically reactive with an antibody raised against a purified pectin lyase derived from Aspergillus aculeatus, CBS 101.43. The enzyme is useful for the degradation of plant cell wall components.
摘要翻译：显示出果胶裂解酶活性的酶，该酶与针对源自Aspergillus aculeatus的纯化果胶裂解酶CBS 101.43产生的抗体具有免疫反应性。该酶可用于植物细胞壁组分的降解。

3. 发明申请

WO1994021785A1 ENZYMES WITH XYLANASE ACTIVITY FROM ASPERGILLUS ACULEATUS 审中-公开
标题翻译：具有来自ASPERGILLUS ACULEATUS的XYLANASE活性的酶
公开(公告)号：WO1994021785A1
公开(公告)日：1994-09-29
申请号：PCT/DK1994000088
申请日：1994-03-02
申请人： NOVO NORDISK A/S , KOFOD, Lene, Venke , KAUPPINEN, Markus, Sakari , CHRISTGAU, Stephan , HELDT-HANSEN, Hans, Peter , DALBOGE, Henrik , ANDERSEN, Lene, Nonboe , SI, Joan, Qi , JACOBSEN, Tina, Sejersgärd , MUNK, Niels , MÜLLERTZ, Anette
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/24
CPC分类号： C12Y302/01032 , A21D8/042 , A23K20/189 , A23K50/75 , A23L2/04 , A23L7/107 , A23L29/06 , C12C5/004 , C12C5/006 , C12C7/28 , C12G1/00 , C12G1/0203 , C12N9/2482 , C12Y302/01008 , D21C5/005
摘要： An enzyme exhibiting xylanase activity, which enzyme is immunologically reactive with an antibody raised against a purified xylanase derived from Aspergillus aculeatus, CBS 101.43. The enzyme may be used for degrading plant cell wall components e.g. in the preparation of feed, in baking, in the paper and pulp industry and in connection with separation of wheat into starch and gluten.
摘要翻译：显示出木聚糖酶活性的酶，该酶与针对源自Aspergillus aculeatus，CBS 101.43的纯化木聚糖酶产生的抗体具有免疫反应性。该酶可用于降解植物细胞壁组分，例如在制备饲料时，在烘烤中，在造纸和纸浆工业中，并将小麦分离成淀粉和面筋。

4. 发明申请

WO1994020612A1 AN ENZYME WITH RHAMNOGALACTURONASE ACTIVITY 审中-公开
标题翻译：具有消炎激酶活性的酶
公开(公告)号：WO1994020612A1
公开(公告)日：1994-09-15
申请号：PCT/DK1994000097
申请日：1994-03-04
申请人： NOVO NORDISK A/S , KOFOD, Lene, Venke , DALBOGE, Henrik , ANDERSEN, Lene, Nonboe , KAUPPINEN, Markus, Sakari , CHRISTGAU, Stephan , HELDT-HANSEN, Hans, Peter , CHRISTOPHERSEN, Claus , NIELSEN, Per, Munk , VORAGEN, Alphons, Gerard, Joseph , SCHOLS, Hendrik, Arie
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/24
CPC分类号： C13B10/083 , A23K20/189 , A23L2/84 , A23L29/06 , C12N9/2402 , C12Y302/01171 , C13K13/00
摘要： An enzyme exhibiting rhamnogalacturonase activity, which enzyme: a) is encoded by the DNA sequence shown in SEQ ID No. 1 or a sequence homologous thereto encoding a polypeptide with RGase activity, b) has the amino acid sequence shown in SEQ ID No. 2 or an analogue thereof, c) is reactive with an antibody raised against the enzyme encoded by the DNA sequence shown in SEQ ID No. 1, d) has a pH optimum above pH 5, and/or e) has a relative activity of at least 30% at a pH in the range of 5.5-6.5. The enzyme may be used for reducing the viscosity of a plant cell wall material and for extracting high molecular weight molecules from a plant cell wall material.
摘要翻译：显示鼠李糖半乳糖醛酸酶活性的酶，其酶：a）由SEQ ID No.1所示的DNA序列或与其同源的序列编码，具有RGase活性的多肽，b）具有SEQ ID No.2所示的氨基酸序列或其类似物，c）与针对由SEQ ID No.1所示的DNA序列编码的酶产生的抗体具有反应性，d）具有优于pH 5以上的pH，和/或e）具有相对活性为在5.5-6.5范围内的pH值至少为30％。该酶可用于降低植物细胞壁材料的粘度并从植物细胞壁材料中提取高分子量分子。

5. 发明申请

WO1995002043A1 DNA ENCODING AN ENZYME WITH ENDOGLUCANASE ACTIVITY FROM TRICHODERMA HARZIANUM 审中-公开
标题翻译：从TRICHODERMA HARZIANUM编码内切酶活性的DNA
公开(公告)号：WO1995002043A1
公开(公告)日：1995-01-19
申请号：PCT/DK1994000275
申请日：1994-07-05
申请人： NOVO NORDISK A/S , DALBOGE, Henrik , CHRISTGAU, Stephan , ANDERSEN, Lene, Nonboe , KOFOD, Lene, Venke , KAUPPINEN, Markus, Sakari
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： C12N9/2437 , C12Y302/01004
摘要： A DNA construct comprising a DNA sequence encoding an enzyme with endoglucanase activity, which DNA sequence comprises the DNA sequence shown in SEQ ID No. 1 or an analogous sequence thereof being at least 70 % homologous to the DNA sequence shown in SEQ ID No. 1. The DNA sequence may be isolated from a strain of Trichoderma harzianum. The endoglucanase expressed from the DNA construct may be used for the degradation or modification of plant materials.
摘要翻译：包含编码具有内切葡聚糖酶活性的酶的DNA序列的DNA构建体，所述DNA序列包含SEQ ID No.1所示的DNA序列或其类似序列与SEQ ID No.1所示的DNA序列至少70％同源 DNA序列可以从哈茨木霉菌株中分离出来。从DNA构建体表达的内切葡聚糖酶可用于植物材料的降解或修饰。

6. 发明申请

WO1995031534A1 AN ENZYME WITH 'beta'-(1-6)-ENDOGLUCANASE ACTIVITY 审中-公开
标题翻译：具有β - （1-6）-ENDOGLUCANASE活性的酶
公开(公告)号：WO1995031534A1
公开(公告)日：1995-11-23
申请号：PCT/DK1995000189
申请日：1995-05-11
申请人： NOVO NORDISK A/S , KOFOD, Lene, Venke , ANDERSEN, Lene, Nonboe , KAUPPINEN, Markus, Sakari , CHRISTGAU, Stephan , DALBOGE, Henrik , OLSEN, Hans, Sejr
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： C12Y302/01075 , C12N9/2402 , Y10S435/945
摘要： The present invention relates to an enzyme with endo-beta-glucanase activity, encoded by a DNA sequence. Further, the invention relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, the use of said enzyme or said preparation for a number of uses including the degradation or modification of beta-glucan containing materials.
摘要翻译：本发明涉及由DNA序列编码的内切β-葡聚糖酶活性的酶。此外，本发明涉及编码该酶的DNA构建体，该酶的制备方法，含该酶的酶制剂，所述酶或所述制剂的用途，包括降解或修饰含有β-葡聚糖的多种用途材料。

7. 发明申请

WO1995002044A1 AN ENZYME WITH PROTEASE ACTIVITY 审中-公开
标题翻译：具有蛋白酶活性的酶
公开(公告)号：WO1995002044A1
公开(公告)日：1995-01-19
申请号：PCT/DK1994000274
申请日：1994-07-05
申请人： NOVO NORDISK A/S , DALBOGE, Henrik , CHRISTGAU, Stephan , ANDERSEN, Lene, Nonboe , KOFOD, Lene, Venke , KAUPPINEN, Markus, Sakari , NIELSEN, Jack, Bech , DAMBMANN, Claus
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/62
CPC分类号： C11D3/3947 , A21D8/042 , A23K20/189 , C11D3/0078 , C11D3/386 , C11D3/38618 , C11D3/38636 , C12N9/62 , C12N9/82
摘要： A DNA construct comprising a DNA sequence encoding an enzyme exhibiting protease activity, which DNA sequence comprises the DNA sequence shown in SEQ ID No. 1 or 2 or an analogue of any of these sequences being at least 80 % homologous to the DNA sequence shown in SEQ ID No. 1 or 2. The proteases encoded by the DNA sequences have an acid pH optimum.
摘要翻译：包含编码表现出蛋白酶活性的酶的DNA序列的DNA构建体，所述DNA序列包含SEQ ID No.1或2所示的DNA序列或这些序列中的任一个的类似物与所示的DNA序列至少80％同源 SEQ ID No.1或2.由DNA序列编码的蛋白酶具有酸性pH最佳值。

8. 发明申请

WO1994020611A1 AN ENZYME WITH ARABINANASE ACTIVITY 审中-公开
标题翻译：具有阿拉伯酶活性的酶
公开(公告)号：WO1994020611A1
公开(公告)日：1994-09-15
申请号：PCT/DK1994000093
申请日：1994-03-04
申请人： NOVO NORDISK A/S , DALBOGE, Henrik , KOFOD, Lene, Venke , KAUPPINEN, Markus, Sakari , ANDERSEN, Lene, Nonboe , CHRISTGAU, Stephan , HELDT-HANSEN, Hans, Peter
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/24
CPC分类号： C12Y302/01099 , C12N9/2402
摘要： An enzyme exhibiting arabinanase activity, which enzyme is derivable from a strain of Aspergillus aculeatus and which enzyme is useful for the degradation of plant cell wall components.
摘要翻译：表现出阿拉伯聚糖酶活性的酶，该酶可从阿曲曲霉菌株中衍生，该酶可用于植物细胞壁成分的降解。

9. 发明申请

WO1994014953A1 AN ENZYME WITH ENDOGLUCANASE ACTIVITY 审中-公开
标题翻译：具有内切酶活性的酶
公开(公告)号：WO1994014953A1
公开(公告)日：1994-07-07
申请号：PCT/DK1993000444
申请日：1993-12-23
申请人： NOVO NORDISK A/S , DALBOEGE, Henrik , ANDERSEN, Lene, Nonboe , KOFOD, Lene, Venke , KAUPPINEN, Markus, Sakari , CHRISTGAU, Stephan
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： C12N9/2437 , C12Y302/01004
摘要： An enzyme exhibiting endoglucanase activity, which enzyme is encoded by a DNA sequence comprising at least one of the following partial sequences: (a) ATTCATTTGTG GACAGTGGAC, (b) GTTGATCGCA CATTGAACCA, (c) ACCCCAGCCG ACCGATTGTC, (d) CTTCCTTACC TCACCATCAT, (e) TTAACATCTT TTCACCATGA, (f) AGCTTTCCCT TCTCTCCCTT, (g) GCCACCCTGG CTTCCGCTGC CAGCCTCC, (h) GACAGTAGCA ATCCAGCATT, (i) AGCATCAGCC GCTTTGTACA, (j) CCATGAAGTT CACCGTATTG, (k) GCACTGCTTC TCTCCCAGGT, (l) GTGGGCGGCC CCTCAGGCAA, (m) ACGCTCCTCC AATTTTCTCT, (n) GGCTTGGTAG TAATGAGTCT, (o) GGCGCAGAGT TTGGCCAGGC, (p) CAACATCCCC GGTGTTCTGGG. The enzyme may be produced by recombinant DNA techniques and may be used for degradation of plant cell wall material.
摘要翻译：显示内切葡聚糖酶活性的酶，其由包含以下部分序列中的至少一种的DNA序列编码：（a）ATTCATTTGTG GACAGTGGAC，（b）GTTGATCGCA CATTGAACCA，（c）ACCCCAGCCG ACCGATTGTC，（d）CTTCCTTACC TCACCATCAT，（e ）TTAACATCTT TTCACCATGA，（f）AGCTTTCCCT TCTCTCCCTT，（g）GCCACCCTGG CTTCCGCTGC CAGCCTCC，（h）GACAGTAGCA ATCCAGCATT，（i）AGCATCAGCC GCTTTGTACA，（j）CCATGAAGTT CACCGTATTG，（k）GCACTGCTTC TCTCCCAGGT，（I）GTGGGCGGCC CCTCAGGCAA，（m） ACGCTCCTCC AATTTTCTCT，（n）GGCTTGGTAG TAATGAGTCT，（o）GGCGCAGAGT TTGGCCAGGC，（p）CAACATCCCC GGTGTTCTGGG。该酶可以通过重组DNA技术产生并且可以用于植物细胞壁材料的降解。

10. 发明申请

WO1994014952A1 AN ENZYME WITH POLYGALACTURONASE ACTIVITY 审中-公开
标题翻译：具有聚糖酶活性的酶
公开(公告)号：WO1994014952A1
公开(公告)日：1994-07-07
申请号：PCT/DK1993000445
申请日：1993-12-23
申请人： NOVO NORDISK A/S , DALBOEGE, Henrik , ANDERSEN, Lene, Nonboe , KOFOD, Lene, Venke , KAUPPINEN, Markus, Sakari , CHRISTGAU, Stephan , HELDT-HANSEN, Hans, Peter , HALKIER, Torben
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/24
CPC分类号： C12G1/0203 , C12N9/2402 , C12Y302/01015
摘要： An enzyme exhibiting polygalacturonase activity, which enzyme is immunologically reactive with an antibody raised against a purified polygalacturonase derived from Aspergillus aculeatus, CBS 101.43. The enzyme may be produced by recombinant DNA techniques and may be used for degradation of plant cell walls, for instance in the wine and juice production.
摘要翻译：显示多聚半乳糖醛酸酶活性的酶，该酶与针对源自Aspergillus aculeatus的纯化多聚半乳糖醛酸酶CBS 101.43产生的抗体具有免疫反应性。该酶可以通过重组DNA技术产生并且可以用于植物细胞壁的降解，例如在葡萄酒和果汁生产中。

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