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    • 10. 发明专利
    • METHOD FOR MEASURING COMPOUND USING MICROBE AS MOLECULE DISCRIMINATING ELEMENT
    • JPH04299995A
    • 1992-10-23
    • JP6660991
    • 1991-03-29
    • NIPPON STEEL CORP
    • OTAKE HISAOKONDO HIROTOSHI
    • C12Q1/06
    • PURPOSE:To measure a stoichiometric amount of a compound in simple and high sensitivity by using a microbe capable of changing the behavior by responding to a specific compound in a test specimen as a molecule discriminating element and taking out behavior change thereof from an optical microscope as a image signal and subjecting the image signal to picture processing. CONSTITUTION:A microbe (e.g. Pseudomonas aeruginos) capable of changing the behavior by responding to a specific compound (e.g. serine) in a test specimen is used as a molecule-discriminating element and suspension of the molecule-discriminating element is attached to the lower face of the upper cover glasses 2 and 12 and liquid drops 6 and 16 of buffer solution are attached to the upper face of lower cover glasses 2 and 12 put on microscope stages 1 and 11 and a test specimen solution is added thereto from capillaries 4 and 14 and upper cover glass 12 is superposed thereon to mix the test specimen solution with the molecule discriminating element and light from a light source 10 is passed through the mixture and image signal is taken out through objective lens of a phase-contrast microscope 17 and the signal is subjected to image analysis using an image analysis system to measure a stoichiometric amount or the specific compound in the test specimen.