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    • 1. 发明申请
    • DEVICE AND METHOD FOR ANALYZING TARGET
    • 用于分析目标的装置和方法
    • US20150056720A1
    • 2015-02-26
    • US14387389
    • 2012-12-12
    • NEC Soft, Ltd.
    • Katsunori HoriiNaoto KanekoJou AkitomiShintarou KatouIwao Waga
    • G01N33/68G01N21/59
    • G01N33/6845C12N9/0065C12Q1/008C12Y111/01007G01N21/59G01N33/5308G01N33/6812
    • The present invention provides a novel sensor for detecting a target. The nucleic acid sensor of the present invention includes a nucleic acid element that includes a catalyst nucleic acid molecule (D) that exerts a catalytic function and a binding nucleic acid molecule (A) that binds to a target. The nucleic acid element is a double-stranded nucleic acid element including a first strand and a second strand. The first strand (ss1) includes the binding nucleic acid molecule (A), a loop-forming sequence (L1), and the catalyst nucleic acid molecule (D) linked in this order. The second strand (ss2) includes a stem-forming sequence (SA), a loop-forming sequence (L2), and a stem-forming sequence (SD) linked in this order. In this nucleic acid element, in the absence of a target, the catalytic function of the catalyst nucleic acid molecule (D) is inhibited by stem formation in each of the stem-forming sequences (SA) and (SD), and in the presence of ATP/target, the stem formation is released by a binding of the binding nucleic acid molecule (A) with the target, and the catalytic function is exerted.
    • 本发明提供一种用于检测目标的新型传感器。 本发明的核酸传感器包括核酸元件,其包含发挥催化功能的催化剂核酸分子(D)和与靶标结合的结合核酸分子(A)。 核酸元件是包含第一链和第二链的双链核酸元件。 第一链(ss1)包括依次连接的结合核酸分子(A),环形成序列(L1)和催化剂核酸分子(D)。 第二链(ss2)包括以此顺序连接的茎形成序列(SA),环形成序列(L2)和茎形成序列(SD)。 在该核酸元件中,在不存在靶的情况下,催化剂核酸分子(D)的催化功能受到每个茎形成序列(SA)和(SD)中的茎形成的抑制,并且在存在 的ATP /靶,通过结合核酸分子(A)与靶标的结合释放茎的形成,并且发挥催化功能。