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1. 发明授权

US07923551B2 Method of purifying RNA using kosmotropic salt 有权
标题翻译：使用去离子水纯化RNA的方法
公开(公告)号：US07923551B2
公开(公告)日：2011-04-12
申请号：US12110391
申请日：2008-04-28
申请人： Myo-yong Lee , Nam Huh , Joon-ho Kim
发明人： Myo-yong Lee , Nam Huh , Joon-ho Kim
IPC分类号： C07H21/00
CPC分类号： C07H21/02 , C12N15/1003 , C12N15/1006
摘要： The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.
摘要翻译：本发明提供了一种纯化RNA的方法，包括使固体支持物与具有含RNA样品的酸性溶液和浓度小于1M的感应性盐接触，从而将RNA与固体支持物结合。根据本发明，由于高RNA产量和低DNA污染，RNA被有效地纯化。本发明在纯化200个或更少核苷酸的RNA中特别有效。

2. 发明申请

US20090131649A1 METHOD OF SEPARATING SMALL RNA MOLECULES USING KOSMOTROPIC SALT 有权
标题翻译：使用KOSMOTROPIC盐分离小RNA分子的方法
公开(公告)号：US20090131649A1
公开(公告)日：2009-05-21
申请号：US12110373
申请日：2008-04-28
申请人： Myo-yong LEE , Nam Huh , Joon-ho Kim
发明人： Myo-yong LEE , Nam Huh , Joon-ho Kim
IPC分类号： C07H1/00
CPC分类号： C12N15/1006
摘要： Disclosed is a method of separating small RNAs of 200 nucleotides or less from larger RNAs on a solid support, using a kosmotropic salt of different concentrations.
摘要翻译：公开了一种使用不同浓度的可变性盐分离从固体支持物上的较大RNA分离200个核苷酸或更少的小RNA的方法。

3. 发明申请

US20090048437A1 METHOD OF PURIFYING RNA USING KOSMOTROPIC SALT 有权
标题翻译：使用KOSMOTROPIC盐净化RNA的方法
公开(公告)号：US20090048437A1
公开(公告)日：2009-02-19
申请号：US12110391
申请日：2008-04-28
申请人： Myo-yong LEE , Nam Huh , Joon-ho Kim
发明人： Myo-yong LEE , Nam Huh , Joon-ho Kim
IPC分类号： C07H21/02
CPC分类号： C07H21/02 , C12N15/1003 , C12N15/1006
摘要： The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.
摘要翻译：本发明提供了一种纯化RNA的方法，包括使固体支持物与具有含RNA样品的酸性溶液和浓度小于1M的感应性盐接触，从而将RNA与固体支持物结合。根据本发明，由于高RNA产量和低DNA污染，RNA被有效地纯化。本发明在纯化200个或更少核苷酸的RNA中特别有效。

4. 发明授权

US08030479B2 Method of separating small RNA molecules using kosmotropic salt 有权
标题翻译：使用去离子水分离小RNA分子的方法
公开(公告)号：US08030479B2
公开(公告)日：2011-10-04
申请号：US12110373
申请日：2008-04-28
申请人： Myo-yong Lee , Nam Huh , Joon-ho Kim
发明人： Myo-yong Lee , Nam Huh , Joon-ho Kim
IPC分类号： C07H21/00
CPC分类号： C12N15/1006
摘要： Disclosed is a method of separating small RNAs of 200 nucleotides or less from larger RNAs on a solid support, using a kosmotropic salt of different concentrations.
摘要翻译：公开了一种使用不同浓度的可变性盐分离从固体支持物上的较大RNA分离200个核苷酸或更少的小RNA的方法。

5. 发明申请

US20060099711A1 Cell lysis method by immobilized metal-ligand complex 失效
标题翻译：固定化金属配体复合物的细胞裂解法
公开(公告)号：US20060099711A1
公开(公告)日：2006-05-11
申请号：US11267005
申请日：2005-11-04
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
IPC分类号： G01N1/00
CPC分类号： C12Q1/6806 , Y10T436/25 , C12Q2523/308
摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.
摘要翻译：提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。此外，由于可以使用各种固体支持体，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

6. 发明授权

US07648838B2 Cell lysis method by immobilized metal-ligand complex 失效
标题翻译：固定化金属配体复合物的细胞裂解法
公开(公告)号：US07648838B2
公开(公告)日：2010-01-19
申请号：US11267005
申请日：2005-11-04
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
IPC分类号： G01N33/48 , G01N33/20 , G01N1/00
CPC分类号： C12Q1/6806 , Y10T436/25 , C12Q2523/308
摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.
摘要翻译：提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。此外，由于可以使用各种固体支持物，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

7. 发明申请

US20120090428A1 Dual Clutch Transmission And Dual Clutch Accuators Thereof 有权
标题翻译：双离合器变速器和双离合器调速器
公开(公告)号：US20120090428A1
公开(公告)日：2012-04-19
申请号：US12903617
申请日：2010-10-13
申请人： Thomas C. Bowen , Nam Huh
发明人： Thomas C. Bowen , Nam Huh
IPC分类号： F16H37/06 , F16D21/00
CPC分类号： F16H3/08 , F16D21/06 , F16D23/12 , F16D2021/0646 , F16D2021/0692 , F16D2023/141 , F16H3/006 , F16H2003/0931
摘要： A dual clutch transmission with multiple gears operatively coupled thereto, comprises: first and second input shafts coaxially arranged to each other; a first clutch operatively coupled with the first input shaft to drive the transmission with a gear selected from a first set of non-consecutively numbered gears; a second clutch coaxially arranged with the first clutch and operatively coupled with the second input shaft to drive the transmission with a gear selected from a second set of non-consecutively numbered gears; first and second clutch actuating devices, each including an actuator and a pivot member, each pivot member operatively coupled between the actuator and a clutch applicator of cylindrical shape to move its clutch applicator for clutch operation; and a plurality of first and second clutch levers received in a clutch retaining housing in a circularly and alternately arranged pattern. The first and second clutch levers are configured to move the first and second clutches, respectively. The cylindrically shaped clutch applicator of the first clutch actuating device is disposed coaxially with the cylindrically shaped clutch applicator of the second clutch actuating device, and the first clutch levers and the second clutch levers can operate independently to each other upon movement of the first clutch applicator or the second clutch applicator to perform a selective clutch operation between the first and second clutches.
摘要翻译：具有与其可操作地连接的多个齿轮的双离合器变速器包括：彼此同轴布置的第一和第二输入轴; 第一离合器，其与第一输入轴可操作地联接，以用选自第一组非连续编号的齿轮的档位来驱动变速器; 与所述第一离合器同轴地布置的第二离合器，并且与所述第二输入轴可操作地联接以利用选自第二组非连续编号的齿轮的档位驱动所述变速器; 第一和第二离合器致动装置，每个包括致动器和枢转构件，每个枢转构件可操作地联接在致动器和圆柱形形式的离合器施加器之间，以移动其离合器施加器用于离合器操作; 多个第一和第二离合器杆以圆形和交替排列的图案容纳在离合器保持壳体中。第一和第二离合器杆分别构造成移动第一和第二离合器。第一离合器致动装置的圆柱形离合器施加器与第二离合器致动装置的圆柱形离合器施加器同轴设置，并且当第一离合器施加器运动时第一离合器杆和第二离合器杆可以彼此独立地操作或第二离合器施加器，以在第一和第二离合器之间执行选择性离合器操作。

8. 发明授权

US07544505B2 Hybridization chamber agitation device using pump and valves 失效
标题翻译：使用泵和阀的杂化室搅拌装置
公开(公告)号：US07544505B2
公开(公告)日：2009-06-09
申请号：US11295844
申请日：2005-12-06
申请人： Sung-ouk Jung , Nam Huh , Soo-suk Lee , Chang-eun Yoo , Hun-joo Lee
发明人： Sung-ouk Jung , Nam Huh , Soo-suk Lee , Chang-eun Yoo , Hun-joo Lee
IPC分类号： C12M1/34
CPC分类号： B01L3/502738 , B01F13/0059 , B01F13/0222 , B01L3/50273 , B01L7/52 , B01L2300/0636 , B01L2300/0822 , B01L2300/0877
摘要： Provided is an agitation device used to agitate a solution in a hybridization chamber, the agitation device including: the hybridization chamber; first and second air channels connected to ends of the hybridization chamber; a first valve disposed in the first air channel; a second valve disposed in the second air channel; an integrated air channel connecting the first and second air channels; and a pump disposed in the integrated air channel. The agitation device is suitable for effective diffusion of a sample when performing hybridization using a DNA chip. Therefore, a probe can be effectively hybridized with a target material.
摘要翻译：提供了用于搅拌杂交室中的溶液的搅拌装置，搅拌装置包括：杂交室; 第一和第二空气通道连接到杂交室的末端; 设置在第一空气通道中的第一阀; 设置在第二空气通道中的第二阀; 连接第一和第二空气通道的集成空气通道; 以及设置在集成空气通道中的泵。搅拌装置适用于使用DNA芯片进行杂交时样品的有效扩散。因此，探针可以有效地与靶材料杂交。

9. 发明申请

US20060246473A1 Primer set capable of specifically amplifying a target sequence found in 10 bacterial species and probe oligonucleotide specifically hybridizable with each target sequence of the 10 bacterial species 有权
公开(公告)号：US20060246473A1
公开(公告)日：2006-11-02
申请号：US11317601
申请日：2005-12-23
申请人： Ji-young Oh , Nam Huh , Sang-hyun Peak , Sung-young Jeong
发明人： Ji-young Oh , Nam Huh , Sang-hyun Peak , Sung-young Jeong
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689
摘要： Provided are a primer set for amplifying a target sequence specific to Bordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Stapylococcus aureus, and Streptococcus pneumoniae, the primer set including at least one oligonucleotide of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 1 and at least one oligonucleotides of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 2; and probes specific to a specific species of the 10 bacterial species.

10. 发明申请

US20060177849A1 Nucleic acid primer set, nucleic acid probe set and method for detecting respiratory disease virus using the primer set and probe set 审中-公开
标题翻译：核酸引物组，核酸探针组和使用引物组和探针组检测呼吸系统疾病病毒的方法
公开(公告)号：US20060177849A1
公开(公告)日：2006-08-10
申请号：US11318240
申请日：2005-12-23
申请人： Ji-young Oh , Nam Huh , Jung-nam Lee
发明人： Ji-young Oh , Nam Huh , Jung-nam Lee
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/701 , C12Q1/705
摘要： Provided are a nucleic acid primer set capable of simultaneously amplifying the target sequences of five or more respiratory disease viruses and including oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in sequences as set forth SEQ ID NOS: 1-26, a nucleic acid probe set for detecting the amplified product and a method of simultaneously detecting five or more respiratory disease viruses using the primer set.
摘要翻译：提供了能够同时扩增五种或更多种呼吸道疾病病毒的靶序列并包括寡核苷酸的核酸引物组，其各自包含以SEQ ID NO：1-26所示的序列存在的至少10个连续核苷酸的片段，用于检测扩增产物的核酸探针组和使用引物组同时检测五种或更多种呼吸道疾病病毒的方法。

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