专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5618715A Oncostatin M and novel compositions having anti-neoplastic activity 失效
标题翻译：制瘤素M和具有抗肿瘤活性的新型组合物
公开(公告)号：US5618715A
公开(公告)日：1997-04-08
申请号：US75199
申请日：1993-06-10
申请人： Mohammed Shoyab , Joyce M. Zarling , Hans Marquardt , Marcia B. Hanson , Najma Malik , Peter S. Linsley , Timothy M. Rose , Anthony F. Purchio
发明人： Mohammed Shoyab , Joyce M. Zarling , Hans Marquardt , Marcia B. Hanson , Najma Malik , Peter S. Linsley , Timothy M. Rose , Anthony F. Purchio
IPC分类号： A61K38/00 , C07K14/07 , C07K14/475 , C07K14/495 , C07K14/52 , C07K16/22 , C07K16/24 , C12N15/62 , C12N15/73 , C12N5/00 , C07H17/00 , C12N15/00 , C12P21/06
CPC分类号： C07K14/005 , A61K38/191 , A61K38/204 , C07K14/475 , C07K14/495 , C07K14/52 , C07K14/522 , C07K16/22 , C07K16/24 , C12N15/625 , C12N15/73 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/75 , C12N2710/24122
摘要： Novel compositions comprising Oncostatin M and congeners thereof, as well as methods for their preparation and methods for their use are provided. The compositions may be prepared by isolation from natural sources, or by recombinant means in either prokaryotic or eukaryotic host cells. In addition, the DNA and polypeptide sequences for Oncostatin M are disclosed. The compositions find use in modulating growth of cells, in particular inhibition of tumor cell proliferation, and stimulation of normal cell growth, especially cells involved in hematopoiesis. Cell growth inhibition compositions may additionally include an adjunctive agent comprising at least one of a transforming growth factor, tumor necrosis factor, or an interferon. Receptors having high affinity for Oncostatin M may additionally be used to screen polypeptides for Oncostatin M-like activity. Methods for use of antibodies to the compositions and probes specific for Oncostatin M mRNA as a means for detecting tumor cells are also provided.
摘要翻译：提供了包含制瘤素M及其同源物的新型组合物，以及其制备方法及其使用方法。组合物可以通过从天然来源或通过重组方法在原核或真核宿主细胞中分离来制备。此外，公开了制瘤素M的DNA和多肽序列。该组合物可用于调节细胞的生长，特别是抑制肿瘤细胞增殖，刺激正常的细胞生长，特别是涉及造血的细胞。细胞生长抑制组合物可另外包括包含转化生长因子，肿瘤坏死因子或干扰素中的至少一种的辅助剂。对制瘤素M具有高亲和力的受体可以另外用于筛选多肽用于制瘤素M样活性。还提供了使用针对制瘤素M mRNA特异性的组合物和探针的抗体作为肿瘤细胞检测手段的方法。

2. 发明授权

US5262177A Recombinant viruses encoding the human melanoma-associated antigen 失效
标题翻译：编码人黑素瘤相关抗原的重组病毒
公开(公告)号：US5262177A
公开(公告)日：1993-11-16
申请号：US7230
申请日：1987-01-27
申请人： Joseph P. Brown , Charles D. Estin , Gregory D. Plowman , Timothy M. Rose , Karl E. Hellstrom , Ingegerd Hellstrom , Anthony F. Purchio , Shiu-Lok Hu , Sridhar Pennathur
发明人： Joseph P. Brown , Charles D. Estin , Gregory D. Plowman , Timothy M. Rose , Karl E. Hellstrom , Ingegerd Hellstrom , Anthony F. Purchio , Shiu-Lok Hu , Sridhar Pennathur
IPC分类号： A61K39/00 , C07K14/79 , C12N1/21 , C12N15/13 , G01N33/574 , A61K39/12 , C12N1/16 , C12N5/00 , C12N7/00 , C12N15/00 , C12P19/34 , C12P21/02
CPC分类号： A61K39/0011 , C07K14/79 , G01N33/5743
摘要： Peptides or proteins related to a melanoma associated antigen are described. These are produced in large quantities via recombinant DNA techniques and/or by chemical synthetic methods. The peptides or proteins can be used as immunogens in vaccine formulations which can induce an immune response that selectively destroys melanoma cells in a vaccinated individual. Where the peptides or proteins are expressed by a recombinant virus, inactivated or live virus vaccine formulations may be prepared.
摘要翻译：描述了与黑素瘤相关抗原相关的肽或蛋白质。这些通过重组DNA技术和/或通过化学合成方法大量生产。肽或蛋白质可用作疫苗制剂中的免疫原，其可以诱导在接种个体中选择性地破坏黑素瘤细胞的免疫应答。当肽或蛋白质由重组病毒表达时，可以制备灭活或活病毒疫苗制剂。

3. 发明授权

US4629363A Continuous conduit laying apparatus 失效
标题翻译：连续导管铺设设备
公开(公告)号：US4629363A
公开(公告)日：1986-12-16
申请号：US667780
申请日：1984-11-02
申请人： Timothy M. Rose , J. Ken Rose
发明人： Timothy M. Rose , J. Ken Rose
IPC分类号： E02F5/10 , E03F3/06 , F16L1/00 , H02G1/06 , E02D29/10
CPC分类号： H02G1/06 , E02F5/101 , E02F5/102 , E02F5/103
摘要： A continuous concrete encased conduit laying apparatus is attached to the rear of a tractor in a cantilevered fashion and can be raised and lowered into the ground by the tractor. The apparatus has two sections which are pivotally attached to each other. The forward section is the gouger, and it has a vertical bar having a sharp leading ripper edge. At the base of the bar are attached a wedge and a cone-shaped expander for creating a cavity in the ground as the apparatus is being pulled through the ground.The rear section which is the conduit layer, has a vertical guide tube, a concrete chute attached behind it, and a second expander forming the base of the layer. There is a hopper for holding the fresh concrete at the top of the chute. A quantity of conduit is fed through the guide tube as the apparatus is in operation. The cavity created by the two expanders is filled with fresh concrete as the conduit is laid in the cavity. The second expander can be round or rectangular in cross-section for holding several conduits, and for creating various cross-section encasements. The method includes the steps of laying the conduit and encasing it with concrete in the cavity.
摘要翻译：连续混凝土包裹导管铺设设备以悬臂方式连接在拖拉机的后部，并可通过拖拉机升高和降低到地面。该装置具有彼此枢转地连接的两个部分。前部是刨刀，它有一个垂直杆，具有锋利的前沿裂土器边缘。在杆的底部附有楔形件和锥形膨胀器，用于在装置被拉过地面时在地面中形成空腔。作为导管层的后部具有垂直的引导管，附着在其后面的混凝土滑槽和形成该层的基部的第二扩展器。有一个垃圾桶，用于将新混凝土保持在滑槽的顶部。当设备运行时，一定数量的导管通过导管进给。当管道铺设在空腔中时，由两个膨胀机产生的空腔充满新鲜的混凝土。第二个膨胀器可以是横截面的圆形或矩形，用于保持多个管道，并且用于产生各种横截面的包装。该方法包括以下步骤：铺设导管并将混凝土包住在腔中。

4. 发明授权

US6051375A Glycoprotein B of the RFHV/KSHV subfamily of herpes viruses 失效
公开(公告)号：US6051375A
公开(公告)日：2000-04-18
申请号：US301390
申请日：1999-04-28
申请人： Timothy M. Rose , Marnix L. Bosch , Kurt Strand
发明人： Timothy M. Rose , Marnix L. Bosch , Kurt Strand
IPC分类号： G01N33/566 , A61K31/00 , A61K38/00 , A61K39/245 , A61K39/395 , A61K39/42 , A61P31/00 , A61P31/12 , C07K14/03 , C07K16/08 , C12N1/21 , C12N15/09 , C12N15/38 , C12P21/02 , C12P21/08 , C12Q1/68 , G01N33/569 , C12Q1/70 , C12P19/34
CPC分类号： C07K14/005 , A61K38/00 , C07K2319/00 , C12N2710/16022 , C12N2710/16422
摘要： This invention relates to polynucleotides encoding Glycoprotein B from the RFHV/KSHV subfamily of gamma herpes viruses, three members of which are characterized in detail. DNA extracts were obtained from Macaque nemestrina and Macaque mulatta monkeys affected with retroperitoneal fibromatosis (RF), and human AIDS patients affected with Kaposi's sarcoma (KS). The extracts were amplified using consensus-degenerate oligonucleotide probes designed from known protein and DNA sequences of gamma herpes viruses. The nucleotide sequences of a 319 base pair fragment are about 76% identical between RFHV1 and KSHV, and about 60-63% identical with the closest related gamma herpes viruses outside the RFHV/KSHV subfamily. Protein sequences encoded within these fragments are are about 91% identical between RFHV1 and KSHV, and

5. 发明授权

US5925733A DNA polymerase of gamma herpes viruses associated with Kaposi's sarcoma and retroperitoneal fibromatosis 失效
标题翻译：与卡波西氏肉瘤和腹膜后纤维瘤病有关的疱疹病毒DNA聚合酶
公开(公告)号：US5925733A
公开(公告)日：1999-07-20
申请号：US680326
申请日：1996-07-11
申请人： Timothy M. Rose , Marnix L. Bosch , Kurt Strand , George J. Todaro
发明人： Timothy M. Rose , Marnix L. Bosch , Kurt Strand , George J. Todaro
IPC分类号： C12N15/09 , A61K31/711 , A61K39/00 , A61K39/245 , A61K48/00 , A61P31/22 , C07K14/03 , C07K16/40 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/12 , C12N15/54 , C12P21/08 , C12Q1/68 , C12Q1/70 , C12R1/91 , C12R1/93 , A61K39/02 , C07H21/04 , C07K1/00 , C12N9/00
CPC分类号： C12N9/1252 , C07K14/005 , C12Q1/705 , A61K39/00 , C07K2319/00 , C12N2710/16022 , C12N2710/16422
摘要： This invention provides isolated polynucleotides encoding DNA polymerases of three members of a subfamily of gamma herpes viruses. Two were obtained from macaque monkeys affected with retroperitoneal fibromatosis, the other from human AIDS patients affected with Kaposi's sarcoma. A 454-base pair fragment encoding a region near the active site of the DNA polymerase is 69-83% identical amongst the three viruses, but only 54-68% identical with other known gamma herpes sequences and
摘要翻译：本发明提供编码γ疱疹病毒亚科三个成员的DNA聚合酶的分离的多核苷酸。 2例患者受到腹膜后纤维瘤病影响的猕猴，另一例来自患有卡波西肉瘤的艾滋病患者。编码DNA聚合酶活性位点附近区域的454碱基对片段在三种病毒中是69-83％相同，但与其他已知的γ疱疹序列只有54-68％相同，与α和β的55％相同疱疹序列。还提供了在RFHV / KSHV亚家族中编码来自相关病毒的DNA聚合酶的多核苷酸。根据序列数据制备的多核苷酸可用作检测和表征相关序列的试剂。这些试剂可用于检测RFHV / KSHV亚家族的成员，包括但不限于RFHV，RFHV2和KSHV。可以通过表达多核苷酸或通过化学合成获得相应的多肽和肽片段。它们可用于检测潜在存在于受感染受试者血清中的特异性抗体。它们也可用于设计或筛选通过抑制DNA聚合酶活性来限制病毒复制的药物化合物。

6. 发明授权

US06204364B1 Hybrid cytokines 失效
标题翻译：杂合细胞因子
公开(公告)号：US06204364B1
公开(公告)日：2001-03-20
申请号：US08097869
申请日：1993-07-27
申请人： George J. Todaro , Timothy M. Rose
发明人： George J. Todaro , Timothy M. Rose
IPC分类号： C07K1400
CPC分类号： C07K14/535 , A61K38/00 , A61K38/2093 , C07K14/52 , C07K14/54 , C07K14/5412 , C07K2319/75
摘要： Hybrid cytokines containing four helical regions, each of which is derived from a corresponding &agr;-helical region in leukemia inhibitory factor (LIF), granulocyte colony stimulating factor (G-CSF), interleukin-6 (IL-6) or oncostatin-M (OSM) are disclosed. These hybrid cytokines may further contain linking regions also derived from corresponding linking regions in these factors. The hybrid cytokines offer a unique spectrum of activities useful in treating conditions for which the native cytokines are useful or in treating conditions characterized by an excess of the native cytokines.
摘要翻译：含有四个螺旋区域的杂合细胞因子，其各自衍生自白血病抑制因子（LIF），粒细胞集落刺激因子（G-CSF），白细胞介素-6（IL-6）或制瘤素-M（ OSM）。这些杂交细胞因子还可以包含也源自这些因子中相应连接区的连接区。杂交细胞因子提供了独特的活性谱，其可用于治疗天然细胞因子有用的条件或治疗以过量天然细胞因子为特征的病症。

7. 发明授权

US06171824B2 Hybrid cytokines 失效
标题翻译：杂合细胞因子
公开(公告)号：US06171824B2
公开(公告)日：2001-01-09
申请号：US08149101
申请日：1993-11-08
申请人： George J. Todaro , David W. Leung , Timothy M. Rose
发明人： George J. Todaro , David W. Leung , Timothy M. Rose
IPC分类号： C12P2106
CPC分类号： C07K14/5412 , A61K38/00 , A61K38/185 , A61K38/2073 , A61K38/2093 , C07K14/475 , C07K14/52 , C07K14/535 , C07K14/54 , C07K2319/00
摘要： Therapeutic hybrid cytokines, having a size ranging from about 10 to about 30 kDa, comprise portions of cytokines: leukemia inhibitory factor (LIF), granulocyte-colony stimulating factor (G-CSF), interleukin-6 (IL-6), interleukin-11 (IL-11), oncostatin-M (OSM), and ciliaryneurotrophic factor (CNTF). Hybrid cytokines comprise three or four &agr;-helical sequences selected from &agr;-helical sequences of IL-6, G-CSF, LIF, IL-11, CNTF and OSM and linking sequences of 5-40 amino acids in length, selected from the linking sequences of IL-6, G-CSF, LIF, IL-11, CNTF and OSM or other, desirable linking sequences. In the hybrid cytokines, at least one &agr;-helical sequence is derived from a different cytokine than at least one other &agr;-helical sequence; or, at least one linking sequence of a cytokine differentiates the hybrid cytokine from a corresponding cytokine. In hybrid cytokines having three &agr;-helical sequences selected from the group of cytokines listed, a possible fourth sequence may not correspond to any &agr;-helical sequence in any of the cytokines. Hybrid cytokines, radically different from any corresponding cytokines, have unexpected advantages in selective biological mechanisms over a much broader range of biological activities than exhibited by cytokines.
摘要翻译：具有约10至约30kDa大小的治疗性杂合细胞因子包括细胞因子的部分：白血病抑制因子（LIF），粒细胞集落刺激因子（G-CSF），白细胞介素-6（IL-6），白细胞介素-6 11（IL-11），制瘤素-M（OSM）和睫状神经营养因子（CNTF）。杂交细胞因子包含三个或四个选自IL-6，G-CSF，LIF，IL-11，CNTF和OSM的α-螺旋序列的α-螺旋序列和长度为5-40个氨基酸的连接序列，其选自连接 IL-6，G-CSF，LIF，IL-11，CNTF和OSM或其它所需连接序列的序列。在杂交细胞因子中，至少一种α-螺旋序列衍生自不同于至少一种其他α-螺旋序列的细胞因子; 或者细胞因子的至少一个连接序列将杂合细胞因子与相应的细胞因子区分开来。在具有选自列出的细胞因子组的三个α-螺旋序列的混合细胞因子中，可能的第四序列可能不对应于任何细胞因子中的任何α-螺旋序列。与任何相应的细胞因子完全不同的杂合细胞因子在选择性生物学机制方面具有比细胞因子显示更广泛的生物活性范围的意想不到的优势。

8. 发明授权

US6022542A Glycoprotein B of the RFHV/KSHV subfamily of herpes viruses 失效
标题翻译：疱疹病毒RFHV / KSHV亚家族的糖蛋白B
公开(公告)号：US6022542A
公开(公告)日：2000-02-08
申请号：US720229
申请日：1996-09-26
申请人： Timothy M. Rose , Marnix L. Bosch , Kurt Strand
发明人： Timothy M. Rose , Marnix L. Bosch , Kurt Strand
IPC分类号： G01N33/566 , A61K31/00 , A61K38/00 , A61K39/245 , A61K39/395 , A61K39/42 , A61P31/00 , A61P31/12 , C07K14/03 , C07K16/08 , C12N1/21 , C12N15/09 , C12N15/38 , C12P21/02 , C12P21/08 , C12Q1/68 , G01N33/569 , A61K39/12
CPC分类号： C07K14/005 , A61K38/00 , C07K2319/00 , C12N2710/16022 , C12N2710/16422
摘要： This invention relates to polynucleotides encoding Glycoprotein B from the RFHV/KSHV subfamily of gamma herpes viruses, three members of which are characterized in detail. DNA extracts were obtained from Macaque nemestrina and Macaque mulatta monkeys affected with retroperitoneal fibromatosis (RF), and human AIDS patients affected with Kaposi's sarcoma (KS). The extracts were amplified using consensus-degenerate oligonucleotide probes designed from known protein and DNA sequences of gamma herpes viruses. The nucleotide sequences of a 319 base pair fragment are about 76% identical between RFHV1 and KSHV, and about 60-63% identical with the closest related gamma herpes viruses outside the RFHV/KSHV subfamily. Protein sequences encoded within these fragments are are about 91% identical between RFHV1 and KSHV, and
摘要翻译：本发明涉及编码来自伽马疱疹病毒的RFHV / KSHV亚家族的糖蛋白B的多核苷酸，其特征在于三个成员。从腹膜后纤维瘤（RF）影响的猕猴和猕猴猕猴获得DNA提取物，以及受卡波西氏肉瘤（KS）影响的人类艾滋病患者。使用由已知蛋白质和γ疱疹病毒DNA序列设计的共有简并寡核苷酸探针扩增提取物。 319碱基对片段的核苷酸序列在RFHV1和KSHV之间约为76％，与RFHV / KSHV亚科以外最接近的相关γ疱疹病毒约60-63％相同。在这些片段中编码的蛋白质序列在RFHV1和KSHV之间具有约91％的相同性，并且与其他γ疱疹病毒的差异相同。全长KSHV糖蛋白B序列包括在N末端附近的跨膜结构域和细胞外结构域中的多个潜在的抗原位点。提供材料和方法来表征RFHV / KSHV亚科成员的糖蛋白B编码区，包括但不限于RFHV1，RFHV2和KSHV。本发明的肽，多核苷酸和抗体可用于诊断感染，并用于引发对糖蛋白B的免疫反应。

9. 发明授权

US6015565A Glycoprotein B of the RFHV/KSHV subfamily of herpes viruses 失效
公开(公告)号：US6015565A
公开(公告)日：1999-01-18
申请号：US804439
申请日：1997-02-21
申请人： Timothy M. Rose , Marnix L. Bosch , Kurt Strand
发明人： Timothy M. Rose , Marnix L. Bosch , Kurt Strand
IPC分类号： A61P31/22 , C07K14/03 , A61K39/245 , A61K39/12 , C12N15/00 , C12P21/06
CPC分类号： C07K14/005 , C12N2710/16422
摘要： This invention relates to polynucleotides encoding Glycoprotein B from the RFHV/KSHV subfamily of gamma herpes viruses, three members of which are characterized in detail. DNA extracts were obtained from Macaque nemestrina and Macaque mulatta monkeys affected with retroperitoneal fibromatosis (RF), and human AIDS patients affected with Kaposi's sarcoma (KS). The extracts were amplified using consensus-degenerate oligonucleotide probes designed from known protein and DNA sequences of gamma herpes viruses. The nucleotide sequences of a 319 base pair fragment are about 76% identical between RFHV1 and KSHV, and about 60-63% identical with the closest related gamma herpes viruses outside the RFHV/KSHV subfamily. Protein sequences encoded within these fragments are are about 91% identical between RFHV1 and KSHV, and

10. 发明授权

US06194200B1 Expression systems for preparation of polypeptides in prokaryotic cells 失效
标题翻译：用于在原核细胞中制备多肽的表达系统
公开(公告)号：US06194200B1
公开(公告)日：2001-02-27
申请号：US07993482
申请日：1992-12-18
申请人： Timothy M. Rose , A. Gregory Bruce
发明人： Timothy M. Rose , A. Gregory Bruce
IPC分类号： C12N1571
CPC分类号： C12N15/70 , C07K2319/02 , C07K2319/50 , C07K2319/75
摘要： Expression cassettes for enhanced expression and production of a polypeptide of interest in prokaryotic cells are provided. The expression cassettes provide for production of the polypeptide of interest so that such polypeptide can either be secreted from the host cell in an active conformation or conveniently processed and renatured to a functional state. Preferably, the polypeptide of interest is expressed as a fusion protein, particularly fused to a leader sequence from a highly expressed bacterial or bacteriophage gene. The polypeptide of interest may subsequently be cleaved from the leader sequence and refolded, or used as a fusion protein.
摘要翻译：提供用于在原核细胞中增强表达和产生感兴趣多肽的表达盒。表达盒提供感兴趣的多肽的生产，使得这样的多肽可以以活性构象从宿主细胞分泌，或者方便地加工和复性至功能状态。优选地，感兴趣的多肽表达为融合蛋白，特别是与来自高度表达的细菌或噬菌体基因的前导序列融合。感兴趣的多肽随后可以从前导序列切割并重折叠，或用作融合蛋白。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式