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1. 发明申请

US20080145922A1 DEVICE FOR PERFORMING ANALYSIS ON CELL CULTURES 有权
公开(公告)号：US20080145922A1
公开(公告)日：2008-06-19
申请号：US11952219
申请日：2007-12-07
申请人： Mirko Lehmann , Martin Brischwein , Werner Baumann , Ralf Ehret , Ingo Freund
发明人： Mirko Lehmann , Martin Brischwein , Werner Baumann , Ralf Ehret , Ingo Freund
IPC分类号： C12M1/00
CPC分类号： C12M41/46 , C12M27/02 , C12M33/04
摘要： A device (1) for the carrying out of investigations on cell cultures (2) which take place in a liquid culture medium (4). The device possesses at least a receptacle (3) to contain the culture medium (4) including the cell culture (2), wherein one or more sensors (6) for measurement of the cell culture activities is provided and wherein the receptacle (3) has at least an opening for the adding and the removal of liquid culture medium (4) and the like. A separating element (7) can be introduced into the open upper part of the receptacle (3) and can be brought within the said receptacle (3) in a position close to the bottom (5), in which the said separating element (7) makes a boundary forming a partial space (8) which has a small volume in relation to the entire volume of the receptacle (3). Further, a flow channel (9) is provided, which first, communicates with the small volume partial space (8), and second, communicates with the said reservoir (14). This flow channel (9) and/or the sensors (6) are placed in the area of the small volume partial space (8). By means of the capability for adjustment of the separating element (7) as to its distance from the bottom of the receptacle (3), in the neighborhood of the cell culture (2) a microreaction volume exactly fitting the current requirements can be adjusted. By means of the flow channel (9), the cell culture (2) in the small volume partial space (8) stands in fluid communication with that in the reservoir. For the regeneration of the cell culture solution in the small volume partial space, the separating element (7) can be elevated and depressed, or it is possible, to inject into the small volume partial space of the receptacle (3) a quantity of liquid and by means of a through-flow channel. In this case, a convective mixing of fresh and spent medium takes place through the flow channel (9) between the small volume partial space and the reservoir (14).

2. 发明授权

US06368851B1 Method and apparatus for measuring a state variable 有权
标题翻译：用于测量状态变量的方法和装置
公开(公告)号：US06368851B1
公开(公告)日：2002-04-09
申请号：US09321458
申请日：1999-05-27
申请人： Werner Baumann , Ralf Ehret , Mirko Lehmann , Günter Igel , Hans-Jürgen Gahle , Bernhard Wolf , Ulrich Sieben , Ingo Freund , Martin Brischwein
发明人： Werner Baumann , Ralf Ehret , Mirko Lehmann , Günter Igel , Hans-Jürgen Gahle , Bernhard Wolf , Ulrich Sieben , Ingo Freund , Martin Brischwein
IPC分类号： C12M142
CPC分类号： C12N15/89 , C12M35/02 , C12M35/04 , C12M35/08 , C12N13/00
摘要： A method is provided for measuring a state variable of a biological cell (3) located in a nutrient medium (2) and supported on and adhering to a support area (5). Within the support area (5) for the cell (3) and at a distance from the support area edge, an opening is made in the membrane of the cell (3). The edge of the cell membrane that surrounds the opening and adheres to the support area (5) seals off the liquid found inside the cell (3) from the nutrient medium (2). Through the opening the state variable (2) is measured. An apparatus for performing the method is also provided.
摘要翻译：提供了一种用于测量位于营养介质（2）中并被支撑并附着到支撑区域（5）上的生物体（3）的状态变量的方法。在电池（3）的支撑区域（5）内并且距离支撑区域边缘一定距离处，在电池（3）的膜中形成开口。围绕开口并附着到支撑区域（5）的细胞膜的边缘将从细胞（3）内发现的液体与营养培养基（2）密封。通过打开状态变量（2）进行测量。还提供了一种用于执行该方法的装置。

3. 发明授权

US07795012B2 Device for performing analysis on cell cultures 有权
标题翻译：用于对细胞培养进行分析的装置
公开(公告)号：US07795012B2
公开(公告)日：2010-09-14
申请号：US11952219
申请日：2007-12-07
申请人： Mirko Lehmann , Martin Brischwein , Werner Baumann , Ralf Ehret , Ingo Freund
发明人： Mirko Lehmann , Martin Brischwein , Werner Baumann , Ralf Ehret , Ingo Freund
IPC分类号： C12M1/34
CPC分类号： C12M41/46 , C12M27/02 , C12M33/04
摘要： A device for investigating activities of cell cultures in a liquid culture medium includes a receptacle for the culture medium, and one or more sensors for measurement of cell culture activities. An opening for adding and removing liquid culture medium is provided. A separating element can be placed close to the receptacle bottom, forming a partial space with a small volume relative to the entire volume. A flow channel that communicates with the small volume and the reservoir and/or the sensors are placed in the small volume partial space. By adjustment of the separating element distance from the receptacle bottom, a microreaction volume exactly fitting the current requirements is obtained. For regeneration of the cell culture solution in the small volume partial space, the separating element can be elevated and depressed, or liquid can be injected into the small volume by the through-flow channel, providing convective mixing of fresh and spent medium.
摘要翻译：用于调查液体培养基中细胞培养物的活性的装置包括用于培养基的容器和用于测量细胞培养活性的一个或多个传感器。提供了用于添加和除去液体培养基的开口。分离元件可以靠近容器底部放置，形成相对于整个体积具有小体积的部分空间。与小容量和储存器和/或传感器连通的流动通道被放置在小体积部分空间中。通过调节与容器底部的分离元件距离，可以获得与当前要求完全一致的微反应体积。为了在小体积部分空间中再生细胞培养液，分离元件可以被升高和降低，或者可以通过通流通道将液体注入到小容积中，从而提供新鲜和废弃的介质的对流混合。

4. 发明授权

US07501276B2 Apparatus for intracellular manipulation of a biological cell 失效
标题翻译：用于细胞内操作生物细胞的装置
公开(公告)号：US07501276B2
公开(公告)日：2009-03-10
申请号：US10202419
申请日：2002-07-23
申请人： Werner Baumann , Ralf Ehret , Mirko Lehmann , Günter Igel , Hans-Jürgen Gahle , Ulrich Sieben , Ingo Freund , Martin Brischwein
发明人： Werner Baumann , Ralf Ehret , Mirko Lehmann , Günter Igel , Hans-Jürgen Gahle , Ulrich Sieben , Ingo Freund , Martin Brischwein
IPC分类号： C12M1/42 , C12N15/87
CPC分类号： C12N15/89 , C12M35/02 , C12M35/04 , C12M35/08 , C12N13/00
摘要： A process is provided for the intracellular manipulation of a biological cell (3) which is positioned adhering to a support area (5) in a culture medium (2). Inside the support area (5) for the cell (3) an opening into the membrane of the cell (3) is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area (5), thus seals off the cell fluid situated in the interior of the cell (3) from the culture medium (2) and insulates the cell fluid against the culture medium (2). The interior of the cell (3) is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier (4) with a support area (5) for adhering the cell and a poration tool (6) for creating the opening in the cell membrane. The poration tool (6) may be any of various chemical, mechanical and/or electrical devices.
摘要翻译：提供了一种用于细胞内操作生物细胞（3）的方法，所述生物细胞（3）定位为粘附在培养基（2）中的支持区域（5）。在电池（3）的支撑区域（5）的内部，与电池（3）的隔膜形成的开口与其支撑边缘间隔开。围绕开口的细胞膜的边缘附着在支撑区域（5）上，从而将位于细胞（3）内部的细胞液体与培养基（2）密封在一起，并使细胞液体抵抗培养物中等（2）。电池（3）的内部通过开口被操纵。还提供了一种用于实现该方法的装置，包括具有用于粘附细胞的支撑区域（5）的物体载体（4）和用于在细胞膜中形成开口的引锭工具（6）。引锭工具（6）可以是各种化学，机械和/或电气装置中的任何一种。

5. 发明授权

US06638743B2 Method for measuring a state variable 失效
公开(公告)号：US06638743B2
公开(公告)日：2003-10-28
申请号：US10119354
申请日：2002-04-09
申请人： Werner Baumann , Ralf Ehret , Mirko Lehmann , Günter Igel , Hans-Jörg Gahle , Bernhard Wolf , Ulrich Sieben , Ingo Freund , Martin Brischwein
发明人： Werner Baumann , Ralf Ehret , Mirko Lehmann , Günter Igel , Hans-Jörg Gahle , Bernhard Wolf , Ulrich Sieben , Ingo Freund , Martin Brischwein
IPC分类号： C12N1300
CPC分类号： C12N15/89 , C12M35/02 , C12M35/04 , C12M35/08 , C12N13/00
摘要： A method is provided for measuring a state variable of a biological cell (3) located in a nutrient medium (2) and supported on and adhering to a support area (5). Within the support area (5) for the cell (3) and at a distance from the support area edge, an opening is made in the membrane of the cell (3). The edge of the cell membrane that surrounds the opening and adheres to the support area (5) seals off the liquid found inside the cell (3) from the nutrient medium (2). Through the opening the state variable (2) is measured. An apparatus for performing the method is also provided.

6. 发明授权

US06475760B1 Method for intracellular manipulation of a biological cell 有权
标题翻译：细胞内操作生物细胞的方法
公开(公告)号：US06475760B1
公开(公告)日：2002-11-05
申请号：US09321051
申请日：1999-05-27
申请人： Werner Baumann , Ralf Ehret , Mirko Lehmann , Günter Igel , Hans-Jürgen Gahle , Bernhard Wolf , Ulrich Sieben , Ingo Freund , Martin Brischwein
发明人： Werner Baumann , Ralf Ehret , Mirko Lehmann , Günter Igel , Hans-Jürgen Gahle , Bernhard Wolf , Ulrich Sieben , Ingo Freund , Martin Brischwein
IPC分类号： C12N1300
CPC分类号： C12N15/89 , C12M35/02 , C12M35/04 , C12M35/08 , C12N13/00
摘要： A process is provided for the intracellular manipulation of a biological cell (3) which is positioned adhering to a support area (5) in a culture medium (2). Inside the support area (5) for the cell (3) an opening into the membrane of the cell (3) is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area (5), thus seals off the cell fluid situated in the interior of the cell (3) from the culture medium (2) and insulates the cell fluid against the culture medium (2). The interior of the cell (3) is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier (4) with a support area (5) for adhering the cell and a poration tool (6) for creating the opening in the cell membrane. The poration tool (6) may be any of various chemical, mechanical and/or electrical devices.
摘要翻译：提供了一种用于细胞内操作生物细胞（3）的方法，所述生物细胞（3）定位为粘附在培养基（2）中的支持区域（5）。在电池（3）的支撑区域（5）的内部，与电池（3）的隔膜形成的开口与其支撑边缘间隔开。围绕开口的细胞膜的边缘附着在支撑区域（5）上，从而将位于细胞（3）内部的细胞液体与培养基（2）密封并使细胞液体抵抗培养物中等（2）。电池（3）的内部通过开口被操纵。还提供了一种用于实现该方法的装置，包括具有用于粘附细胞的支撑区域（5）的物体载体（4）和用于在细胞膜中形成开口的引锭工具（6）。引锭工具（6）可以是各种化学，机械和/或电气装置中的任何一种。

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