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1. 发明授权

US10578607B2 Method of screening, isolating, and purifying rare cells 有权
公开(公告)号：US10578607B2
公开(公告)日：2020-03-03
申请号：US15817431
申请日：2017-11-20
申请人： Min-Hsien Wu , Wen-Pin Chou , Tzu-Keng Chiu , Po-Yu Chu
发明人： Min-Hsien Wu , Wen-Pin Chou , Tzu-Keng Chiu , Po-Yu Chu
IPC分类号： G01N33/49 , C12N5/09 , G01N27/327 , G01N33/574 , G01N27/447 , C12N13/00
摘要： A method of screening, isolating and purifying rare cells includes obtaining cell groups with rare cells from a sample, removing cells not to be screened from the cell groups, and drawing the remaining cells as a cell suspension sample. An introduction device is activated to load the cell suspension sample onto an optically-induced dielectrophoresis chip including a cell isolation zone having a division arrangement wherein at least one optical pattern is configured on the cell isolation zone for screening, isolating and purifying the rare cells. The at least one optical pattern is configured to migrate, rotate or both. The at least one optical pattern is activated to attract the rare cells into a collection tank on the chip, and a liquid drawing device is activated to draw the rare cells out of the collection tank, thereby obtaining purified rare cells from the sample.

2. 发明申请

US20190154657A1 Method of screening, Isolating, and Purifying Rare Cells by Using Dynamic Optical Patterns and Optically-Induced Dielectrophoresis 审中-公开
公开(公告)号：US20190154657A1
公开(公告)日：2019-05-23
申请号：US15817431
申请日：2017-11-20
申请人： Min-Hsien Wu , Wen-Pin Chou , Tzu-Keng Chiu , Po-Yu Chu
发明人： Min-Hsien Wu , Wen-Pin Chou , Tzu-Keng Chiu , Po-Yu Chu
IPC分类号： G01N33/49 , C12N5/09 , C12N13/00 , G01N33/574 , G01N27/447 , G01N27/327
摘要： A method of screening, isolating and purifying rare cells includes obtaining cell groups with rare cells in from a sample, removing cells not to be screened from the cell groups, and drawing the remaining cells as cell suspension sample; activating an introduction device to load the cell suspension sample onto an optically-induced dielectrophoresis chip including a cell isolation zone having a division arrangement wherein at least one optical pattern is configured on the cell isolation zone for screening, isolating and purifying the rare cells, and the at least one optical pattern is configured to migrate, rotate or both; and activating the at least one optical pattern to attract the rare cells into a collection tank on the chip, and activating a liquid drawing device to draw the rare cells out of the collection tank, thereby obtaining purified rare cells from the sample.

3. 发明授权

US11231425B2 Immunoassay for simultaneously screening anti-platelet antibodies and performing platelets cross matching and test apparatus for carrying out same 有权
公开(公告)号：US11231425B2
公开(公告)日：2022-01-25
申请号：US16567624
申请日：2019-09-11
申请人： Tzong-Shi Chiueh , Min-Hsien Wu , Hsin-Yao Wang
发明人： Tzong-Shi Chiueh , Min-Hsien Wu , Hsin-Yao Wang
IPC分类号： G01N33/68 , G01N33/554 , G01N33/569
摘要： An immunoassay includes forming first mixtures by reacting a combination reagent with serum of a subject; simultaneously forming second mixtures by reacting randomly selected platelet samples with the serum wherein in each mixture there are immunity compounds formed by combining the platelet antigens with predetermined antibodies in the serum, and other platelet antigens and other antibodies in the serum not forming the immunity compounds; preparing an interception device including receptacles and a filter net; placing each mixture in one receptacle; washing the mixtures wherein the mixtures forming the immunity compounds are intercepted by the filter net with others passing through; adding a signal sensing reagent to each receptacle; reacting the signal sensing reagent with the intercepted mixtures forming the immunity compounds to form final products; and performing a signal sensing to determine whether the final products contain anti-platelet antibodies and determine compatibility of cross matching of respective platelet samples.

4. 发明申请

US20120231976A1 Microfluidic Chip for High-Throughput Perfusion-Based Three-Dimensional Cell Culture 有权
标题翻译：用于高通量灌注的三维细胞培养的微流控芯片
公开(公告)号：US20120231976A1
公开(公告)日：2012-09-13
申请号：US13241123
申请日：2011-09-22
申请人： Min-Hsien Wu , Shih-Siou Wang , Wan-Chen Tsai , Yu-Han Chang , Chia-Hsun Hsieh , Yen-Ting Liu , Zhanfeng Cui
发明人： Min-Hsien Wu , Shih-Siou Wang , Wan-Chen Tsai , Yu-Han Chang , Chia-Hsun Hsieh , Yen-Ting Liu , Zhanfeng Cui
IPC分类号： C40B60/14
CPC分类号： C40B60/12 , C12M23/16 , C12M41/00 , C12M41/12 , C12M41/40
摘要： A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.
摘要翻译：用于具有高通量灌注的三维细胞培养的微流体芯片包括一系列细胞培养单元，每个单元包括连接到一个细胞培养基罐的细胞培养基入口孔，至少一个微生物反应器，至少一个微通道和至少一个介质收集和分析罐。每个介质收集和分析罐连接到具有空气通道的空气室，并且空气室具有负压源孔以产生负压以驱动培养基。微流体芯片还包括连接到屋顶底面的中间板，以及可拆卸地组装在中间板的底部的两个底板。第一和第二底面具有微生物反应器和圆柱形凹槽，并且中间板具有相应的孔，以使用具有高通量灌注的最小实验资源实现三维细胞培养的目标。

5. 发明申请

US20110217751A1 Alginate microbeads, method for forming the same and associated applications 审中-公开
标题翻译：藻酸盐微珠，其形成方法和相关应用
公开(公告)号：US20110217751A1
公开(公告)日：2011-09-08
申请号：US13064006
申请日：2011-03-02
申请人： Gwo-Bin Lee , Song-Bin Huang , Min-Hsien Wu
发明人： Gwo-Bin Lee , Song-Bin Huang , Min-Hsien Wu
IPC分类号： C12N11/10 , C08B37/04 , H01F1/00 , B82Y30/00
CPC分类号： C12N11/10 , B82Y30/00 , H01F1/00
摘要： This present invention relates to a method for forming alginate microbeads. The method includes using a needle connected to a vibrator to continuously spot tiny alginate microdroplets in an oil layer. Subsequently, the temporarily formed alginate microdroplets sink into a CaCl2 solution to become gelled microbeads. As a whole, the method has opened up a route to perform alginate-microbead formation in a simple, continuous, controllable, uniform, cell-friendly, and less-contaminated manner.
摘要翻译：本发明涉及一种形成藻酸盐微珠的方法。该方法包括使用连接到振动器的针连续地在油层中发现微小的藻酸盐微滴。随后，临时形成的藻酸盐微滴沉降成CaCl 2溶液成为胶凝微珠。总体而言，该方法开辟了以简单，连续，可控，均匀，细胞友好且污染少的方式进行藻酸盐 - 微珠形成的途径。

6. 发明授权

US09068281B2 Microfluidic chip for high-throughput perfusion-based three-dimensional cell culture 有权
标题翻译：微流控芯片用于高通量灌注型三维细胞培养
公开(公告)号：US09068281B2
公开(公告)日：2015-06-30
申请号：US13241123
申请日：2011-09-22
申请人： Min-Hsien Wu , Shih-Siou Wang , Wan-Chen Tsai , Yu-Han Chang , Chia-Hsun Hsieh , Yen-Ting Liu , Zhanfeng Cui
发明人： Min-Hsien Wu , Shih-Siou Wang , Wan-Chen Tsai , Yu-Han Chang , Chia-Hsun Hsieh , Yen-Ting Liu , Zhanfeng Cui
IPC分类号： C40B60/12 , C12M3/06 , C12M1/34
CPC分类号： C40B60/12 , C12M23/16 , C12M41/00 , C12M41/12 , C12M41/40
摘要： A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.
摘要翻译：用于具有高通量灌注的三维细胞培养的微流体芯片包括一系列细胞培养单元，每个单元包括连接到一个细胞培养基罐的细胞培养基入口孔，至少一个微生物反应器，至少一个微通道和至少一个介质收集和分析罐。每个介质收集和分析罐连接到具有空气通道的空气室，并且空气室具有负压源孔以产生负压以驱动培养基。微流体芯片还包括连接到屋顶底面的中间板，以及可拆卸地组装在中间板的底部的两个底板。第一和第二底面具有微生物反应器和圆柱形凹槽，并且中间板具有相应的孔，以使用具有高通量灌注的最小实验资源实现三维细胞培养的目标。

7. 发明授权

US08551771B2 Apparatuses and methods for gel molding and culture 有权
标题翻译：用于凝胶成型和培养的装置和方法
公开(公告)号：US08551771B2
公开(公告)日：2013-10-08
申请号：US12653335
申请日：2009-12-10
申请人： Min-Hsien Wu , Wan-Chen Tsai
发明人： Min-Hsien Wu , Wan-Chen Tsai
IPC分类号： C12M1/36 , C12M1/38 , C12M3/00 , C12M1/12 , C12M1/22
CPC分类号： C12M25/14 , C12M23/12
摘要： A gel molding apparatus is adapted to be used in combination with a vessel that has a plurality of solution chambers, and includes a lid plate and a plurality of columns that project from a top surface of the lid plate. Each of the columns has a bottom side connected to the top surface of the lid plate, a top side opposite to the bottom side, and a well recessed from the top side for receiving a gel suspension and having a depth from the top side. A method for gel molding is conducted via the gel molding apparatus. A culture apparatus includes the vessel and the gel molding apparatus. A method for culture is performed through the culture apparatus.
摘要翻译：凝胶成型装置适于与具有多个溶液室的容器组合使用，并且包括盖板和从盖板的顶表面突出的多个柱。每个柱具有连接到盖板的顶表面的底侧，与底侧相对的顶侧，以及从顶侧凹入的用于接收凝胶悬浮体并且具有从顶侧的深度的凹部。通过凝胶成型装置进行凝胶成型的方法。培养装置包括容器和凝胶成型装置。通过培养装置进行培养的方法。

8. 发明授权

US08441080B2 Sensing device 有权
标题翻译：感应装置
公开(公告)号：US08441080B2
公开(公告)日：2013-05-14
申请号：US12793647
申请日：2010-06-03
申请人： Tung-Ming Pan , Min-Hsien Wu , Ming-De Huang , Chao-Sung Lai
发明人： Tung-Ming Pan , Min-Hsien Wu , Ming-De Huang , Chao-Sung Lai
IPC分类号： H01L27/14
CPC分类号： A61B5/14539 , C12Q1/001 , G01N27/4145
摘要： A sensing device includes: a semiconductor layer of a field effect semiconductor having upper and lower surfaces; a conductive layer formed on the lower surface of the semiconductor layer; and a sensor layer of an insulator formed on the upper surface of the semiconductor layer. The insulator is made from lanthanide-titanium oxide.
摘要翻译：感测装置包括：具有上表面和下表面的场效应半导体的半导体层; 形成在所述半导体层的下表面上的导电层; 以及形成在半导体层的上表面上的绝缘体的传感器层。绝缘体由镧系元素 - 氧化钛制成。

9. 发明申请

US20100285558A1 Apparatus and Method for High-Throughput Micro-Cell Culture with Mechanical Stimulation 审中-公开
标题翻译：用于机械刺激的高通量微细胞培养的装置和方法
公开(公告)号：US20100285558A1
公开(公告)日：2010-11-11
申请号：US12437803
申请日：2009-05-08
申请人： Min-Hsien Wu , Wei-Chung Pan , Chun-Yen Kuo
发明人： Min-Hsien Wu , Wei-Chung Pan , Chun-Yen Kuo
IPC分类号： C12N13/00 , C12M1/00
CPC分类号： C12N13/00 , C12M29/10 , C12M29/14 , C12M35/04
摘要： A method for high-throughput micro-cell culture with mechanical stimulation includes providing cells on a membrane, supplying a culture medium to the cells, and vibrating the membrane by exerting and varying a fluid pressure on the membrane such that the cells are mechanically stimulated through the membrane.An apparatus for high-throughput micro-cell culture with mechanical stimulation includes a cell culture vessel and a fluid pressure supply unit. The cell culture vessel has the membrane, a culture medium chamber, and a pressure chamber. The fluid pressure supply unit is connected fluidly to the pressure chamber, and has a fluid pressure supply device and a control device. The membrane vibrates when the fluid pressure in the pressure chamber is varied. The apparatus provides mechanical stimulation to the cultured cells in a controllable manner.
摘要翻译：具有机械刺激的高通量微细胞培养的方法包括在膜上提供细胞，向细胞提供培养基，并通过施加和改变膜上的流体压力来振动膜，使得细胞通过机械刺激通过膜。用于具有机械刺激的高通量微细胞培养的装置包括细胞培养容器和流体压力供应单元。细胞培养容器具有膜，培养基室和压力室。流体压力供给单元与压力室流体连接，具有流体压力供给装置和控制装置。当压力室中的流体压力变化时，膜振动。该装置以可控的方式向培养的细胞提供机械刺激。

10. 发明申请

US20220025426A1 METHOD OF IDENTIFYING PROPERTIES OF MICROORGANISMS USING ODEP FORCE 有权
公开(公告)号：US20220025426A1
公开(公告)日：2022-01-27
申请号：US17168328
申请日：2021-02-05
申请人： Jang-Jih Lu , Min-Hsien Wu , Hsin-Yao Wang
发明人： Jang-Jih Lu , Min-Hsien Wu , Hsin-Yao Wang
IPC分类号： C12Q1/04
摘要： A method of identifying properties of microorganisms using ODEP force includes obtaining a sample solution of microorganisms including microorganisms having predetermined properties; pre-processing the sample solution of microorganisms to obtain a sample solution of microorganisms to be tested having predetermined electrical properties; placing the sample solution of microorganisms to be tested on a channel of a chip member; activating a light image module having combinations of different strengths of ODEP force to analyze the sample solution of microorganisms to be tested on the channel in which microorganisms in the sample solution of microorganisms to be tested are configured to generate a predetermined distribution and a predetermined presentation due to the predetermined electrical properties; and identifying properties of the microorganisms based on the predetermined distribution and the predetermined presentation.

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