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1. 发明申请

US20110229966A1 Nanoparticle-Based Gene Delivery Systems 有权
标题翻译：基于纳米粒子的基因递送系统
公开(公告)号：US20110229966A1
公开(公告)日：2011-09-22
申请号：US12809996
申请日：2010-03-18
申请人： Min Su Han , Kangseok Lee , Dong-Eun Kim
发明人： Min Su Han , Kangseok Lee , Dong-Eun Kim
IPC分类号： C12N5/00
CPC分类号： C12N15/113 , A61K31/7105 , A61K31/713 , A61K47/6923 , C12N15/111 , C12N15/87 , C12N15/89 , C12N2310/11 , C12N2310/127 , C12N2310/14 , C12N2310/351 , C12N2310/51 , C12N2310/531 , C12N2320/32 , Y10S977/773
摘要： The present invention provides a gene delivery system containing nanoparticles. In more detail, the present invention provides a gene delivery system containing (a) a nanomaterial; (b) an oligonucleotide as an universal binding partner covalently linked to the surface of the nanomaterial; and (c) a cargo comprising (i) a complementary oligonucleotide containing a nucleotide sequence complementary to the universal binding partner as a binding counter-partner, and (ii) an inhibitory molecule having a nucleotide sequence complementary to a target gene of interest to be inhibited or an inducible molecule having a nucleotide sequence of a target gene of interest to be expressed. The present invention is a gene delivery system capable of feasibly deliver aptamer, siRNA, shRNA, miRNA, ribozyme, DNAzyme, PNA or gene as well as antisense oligonucleotide into the cells. In addition, the present invention is more efficient than the commercially available gene transfer reagent in respect to the degree of knockdown of target protein expression.
摘要翻译：本发明提供含有纳米颗粒的基因递送系统。更详细地，本发明提供了一种基因递送系统，其包含（a）纳米材料; （b）与纳米材料的表面共价连接的通用结合配偶体的寡核苷酸; （c）货物，其包含（i）含有与通用结合配偶体互补的核苷酸序列作为结合对配体的互补寡核苷酸，和（ii）具有与目的靶基因互补的核苷酸序列的抑制性分子，或具有待表达的靶基因的核苷酸序列的诱导型分子。本发明是能够将适体，siRNA，shRNA，miRNA，核酶，DNA酶，PNA或基因以及反义寡核苷酸可行地递送到细胞中的基因递送系统。此外，与靶蛋白质表达的敲低程度相比，本发明比市售的基因转移试剂更有效。

2. 发明授权

US08871509B2 Nanoparticle-based gene delivery systems 有权
标题翻译：基于纳米粒子的基因传递系统
公开(公告)号：US08871509B2
公开(公告)日：2014-10-28
申请号：US12809996
申请日：2010-03-18
申请人： Min Su Han , Kangseok Lee , Dong-Eun Kim
发明人： Min Su Han , Kangseok Lee , Dong-Eun Kim
IPC分类号： C12N5/00 , C12N5/02 , C07H21/02 , C12N15/11 , C12N15/87 , C12N15/113 , C12N15/89 , A61K47/48 , A61K31/713 , A61K31/7105
CPC分类号： C12N15/113 , A61K31/7105 , A61K31/713 , A61K47/6923 , C12N15/111 , C12N15/87 , C12N15/89 , C12N2310/11 , C12N2310/127 , C12N2310/14 , C12N2310/351 , C12N2310/51 , C12N2310/531 , C12N2320/32 , Y10S977/773
摘要： The present invention provides a gene delivery system containing nanoparticles. In more detail, the present invention provides a gene delivery system containing (a) a nanomaterial; (b) an oligonucleotide as an universal binding partner covalently linked to the surface of the nanomaterial; and (c) a cargo comprising (i) a complementary oligonucleotide containing a nucleotide sequence complementary to the universal binding partner as a binding counter-partner, and (ii) an inhibitory molecule having a nucleotide sequence complementary to a target gene of interest to be inhibited or an inducible molecule having a nucleotide sequence of a target gene of interest to be expressed. The present invention is a gene delivery system capable of feasibly deliver aptamer, siRNA, shRNA, miRNA, ribozyme, DNAzyme, PNA or gene as well as antisense oligonucleotide into the cells. In addition, the present invention is more efficient than the commercially available gene transfer reagent in respect to the degree of knockdown of target protein expression.
摘要翻译：本发明提供含有纳米颗粒的基因递送系统。更详细地，本发明提供了一种基因递送系统，其包含（a）纳米材料; （b）与纳米材料的表面共价连接的通用结合配偶体的寡核苷酸; （c）货物，其包含（i）含有与通用结合配偶体互补的核苷酸序列作为结合对配体的互补寡核苷酸，和（ii）具有与目的靶基因互补的核苷酸序列的抑制性分子，或具有待表达的靶基因的核苷酸序列的诱导型分子。本发明是能够将适体，siRNA，shRNA，miRNA，核酶，DNA酶，PNA或基因以及反义寡核苷酸可行地递送到细胞中的基因递送系统。此外，与靶蛋白质表达的敲低程度相比，本发明比市售的基因转移试剂更有效。

3. 发明授权

US07514109B2 Method for producing koji of hot pepper paste (gochujang) with reduced hotness, koji produced by the method, and hot pepper paste prepared from the koji 有权
标题翻译：用该方法生产的辣椒糊（gochujang），由该方法生产的曲，和由曲曲制备的辣椒糊的方法
公开(公告)号：US07514109B2
公开(公告)日：2009-04-07
申请号：US10411751
申请日：2003-04-10
申请人： Seung Jin Lee , Min Su Han , Yeong Il Chang
发明人： Seung Jin Lee , Min Su Han , Yeong Il Chang
IPC分类号： A23L1/22 , A23K1/00
CPC分类号： A23L27/10 , A23L7/104 , A23L11/09 , A23L11/37
摘要： A method for producing a koji of a hot pepper paste with reduced capsaicin and/or dihydrocapsaicin content through action of enzymes secreted from a koji fungus includes adding a koji fungus to starting material for preparation of koji of hot pepper paste, and adding a predetermined amount of hot peppers one or more times to the material during the cultivating step performed by enzymes secreted from the koji fungus. The method can reduce the hotness of a hot pepper paste, while maintaining the intrinsic colors of traditional hot pepper pastes. Accordingly, it is possible to prepare various hot pepper paste products satisfying customer's diverse needs.
摘要翻译：通过从曲木真菌分泌的酶的作用产生辣椒素和/或二氢辣椒素含量降低的辣椒糊的曲的方法包括将曲霉真菌添加到用于制备辣椒酱的曲的原料中，并加入预定量的辣椒一次或多次，由从曲木真菌分泌的酶进行的培养步骤中的材料。该方法可以降低辣椒酱的热度，同时保持传统辣椒酱的固有颜色。因此，可以制备满足顾客多种需求的各种辣椒糊产品。

4. 发明授权

US08600261B2 Developing unit and image forming apparatus having the same 有权
标题翻译：显影单元及其成像装置
公开(公告)号：US08600261B2
公开(公告)日：2013-12-03
申请号：US12632205
申请日：2009-12-07
申请人： Min Su Han
发明人： Min Su Han
IPC分类号： G03G15/08
CPC分类号： G03G21/1832 , G03G15/0882 , G03G21/181
摘要： Disclosed are a developing unit and an image forming apparatus having the same. The developing unit includes a partition frame provided separately from the housing of the developing unit. The partition frame is supported within the housing of the developing unit in such a manner to define a path of movement of developer within the housing through an opening formed in the partition frame. A removable sealing member is detachably arranged on the partition frame to prevent developer leakage during storage or transporting of the developing unit. The installation of the removable sealing member is made easier by the partition frame provided separately from the housing.
摘要翻译：公开了一种显影单元和具有该显影单元的图像形成装置。显影单元包括与显影单元的壳体分开设置的分隔框架。分隔框架以这样的方式被支撑在显影单元的壳体内，即通过形成在分隔框架中的开口限定显影剂在壳体内移动的路径。可移除的密封构件可拆卸地布置在分隔框架上，以防止在显影单元的储存或运输期间显影剂泄漏。通过与壳体分开设置的分隔框架，更容易地安装可拆卸的密封构件。

5. 发明申请

US20100068817A1 COLORIMETRIC DETECTION OF METALLIC IONS IN AQUEOUS MEDIA USING FUNCTIONALIZED NANOPARTICLES 审中-公开
标题翻译：使用功能化纳米粒子在水性介质中金属离子的颜色检测
公开(公告)号：US20100068817A1
公开(公告)日：2010-03-18
申请号：US12513246
申请日：2007-11-08
申请人： Chad A. Mirkin , Jae-Seung Lee , Min Su Han
发明人： Chad A. Mirkin , Jae-Seung Lee , Min Su Han
IPC分类号： G01N33/20
CPC分类号： C12Q1/6813 , C12Q2565/101 , C12Q2563/155 , C12Q2527/107
摘要： Disclosed herein are methods of detecting metal ions in a sample using functionalized nanoparticles. More specifically, functionalized nanoparticles are used to selectively detect metal ions in a sample using changes in melting temperature of hybridized oligonucleotides on functionalized nanoparticles. The melting temperature can be detected using absorbance, color change, or both. In some cases, the concentration of the metal ion in the sample can be determined. Concentrations of metal ion of as little as 20 ppb (about 100 nM) can be detected using the disclosed methods.
摘要翻译：本文公开了使用官能化纳米颗粒检测样品中的金属离子的方法。更具体地，官能化纳米粒子被用于使用官能化纳米粒子上的杂交寡核苷酸的解链温度的变化来选择性地检测样品中的金属离子。可以使用吸光度，颜色变化或两者来检测熔解温度。在某些情况下，可以确定样品中金属离子的浓度。使用所公开的方法可以检测到少至20ppb（约100nM）的金属离子浓度。

6. 发明申请

US20090221095A1 Colorimetric Screening of DNA Binding/Intercalating Agents with Gold Nanoparticle Probes 审中-公开
标题翻译：用金纳米颗粒探针对DNA结合/插入剂进行比色筛选
公开(公告)号：US20090221095A1
公开(公告)日：2009-09-03
申请号：US12090065
申请日：2006-10-13
申请人： Chad A. Mirkin , Min Su Han , Abigail K.R. Lytton-Jean
发明人： Chad A. Mirkin , Min Su Han , Abigail K.R. Lytton-Jean
IPC分类号： G01N33/566
CPC分类号： C12Q1/6813 , C12Q2527/107 , C12Q2563/155 , C12Q2565/601 , C12Q2565/628
摘要： Methods are provided to identify and characterize compounds that bind with duplex and triplex polynucleotides.
摘要翻译：提供方法来鉴定和表征与双链体和三重多核苷酸结合的化合物。

7. 发明申请

US20080311669A1 SCREENING SEQUENCE SELECTIVITY OF OLIGONUCLEOTIDE-BINDING MOLECULES USING NANOPARTICLE BASED COLORIMETRIC ASSAY 有权
标题翻译：使用基于纳米尺度色彩测定的寡核苷酸结合分子的筛选序列选择性
公开(公告)号：US20080311669A1
公开(公告)日：2008-12-18
申请号：US12133243
申请日：2008-06-04
申请人： Chad A. Mirkin , Sarah J. Hurst , Min Su Han , Abigail K.R. Lytton-Jean
发明人： Chad A. Mirkin , Sarah J. Hurst , Min Su Han , Abigail K.R. Lytton-Jean
IPC分类号： G01N33/50
CPC分类号： B82Y30/00 , C12Q1/6811 , Y10S977/704 , Y10S977/924 , Y10T436/143333 , C12Q2525/301
摘要： Disclosed herein are methods of screening sequence selectivity of oligonucleotide-binding molecules using a gold nanoparticle based calorimetric assay.
摘要翻译：本文公开了使用基于金纳米颗粒的量热测定来筛选寡核苷酸结合分子的序列选择性的方法。

8. 发明授权

US07807372B2 Screening sequence selectivity of oligonucleotide-binding molecules using nanoparticle based colorimetric assay 有权
标题翻译：使用基于纳米粒子的比色法测定寡核苷酸结合分子的筛选序列选择性
公开(公告)号：US07807372B2
公开(公告)日：2010-10-05
申请号：US12133243
申请日：2008-06-04
申请人： Chad A. Mirkin , Sarah J. Hurst , Min Su Han , Abigail K. R. Lytton-Jean
发明人： Chad A. Mirkin , Sarah J. Hurst , Min Su Han , Abigail K. R. Lytton-Jean
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： B82Y30/00 , C12Q1/6811 , Y10S977/704 , Y10S977/924 , Y10T436/143333 , C12Q2525/301
摘要： Disclosed herein are methods of screening sequence selectivity of oligonucleotide-binding molecules using a gold nanoparticle based colorimetric assay.
摘要翻译：本文公开了使用基于金纳米颗粒的比色测定法筛选寡核苷酸结合分子的序列选择性的方法。

9. 发明申请

US20090155785A1 REAL-TIME COLORIMETRIC SCREENING INHIBITORS OF ENDONUCLEASE WITH GOLD NANOPARTICLE SUBSTRATE 审中-公开
标题翻译：具有金纳米晶基质的内切酶的实时彩色荧光筛选抑制剂
公开(公告)号：US20090155785A1
公开(公告)日：2009-06-18
申请号：US12020506
申请日：2008-01-25
申请人： Chad A. Mirkin , Xiaoyang Xu , Min Su Han
发明人： Chad A. Mirkin , Xiaoyang Xu , Min Su Han
IPC分类号： C12Q1/68
CPC分类号： G01N33/553 , B82Y5/00 , C12Q1/34 , G01N33/587
摘要： The invention provides methods for screening a compound for its effect on endonuclease activity. The methods comprise providing a compound to be screened utilizing a gold nanoparticle aggregate as the substrate for the endonuclease. The gold nanoparticle aggregate is formed by the hybridization of oligonucleotides attached to the nanoparticles, with or without the presence of a third linker oligonucleotide. The hybridized oligonucleotide duplex serves as a substrate for the endonuclease. A detectable change is brought about in the presence of the endonuclease activity. A decrease in the detectable change reflects the reduced levels of endonuclease activities as a result of the effects of endonuclease inhibitors. The present invention also provides kits for screening an endonuclease inhibitor.
摘要翻译：本发明提供了筛选化合物对其对核酸内切酶活性的影响的方法。所述方法包括使用金纳米颗粒聚集体作为内切核酸酶的底物提供待筛选的化合物。金纳米颗粒聚集体通过与或不与第三接头寡核苷酸的存在连接到纳米颗粒上的寡核苷酸的杂交形成。杂交的寡核苷酸双链体用作内切核酸酶的底物。在存在核酸内切酶活性的情况下可以发现可检测的变化。可检测变化的降低反映了由于内切核酸酶抑制剂的作用而导致的内切核酸酶活性的降低。本发明还提供了用于筛选内切核酸酶抑制剂的试剂盒。

10. 发明申请

US20100021894A1 Nanoparticle-Based Colorimetric Detection Of Cysteine 审中-公开
标题翻译：基于纳米粒子的半胱氨酸比色检测
公开(公告)号：US20100021894A1
公开(公告)日：2010-01-28
申请号：US12340556
申请日：2008-12-19
申请人： Chad A. Mirkin , Jae-Seung Lee , Min Su Han , Pirmin A. Ulmann
发明人： Chad A. Mirkin , Jae-Seung Lee , Min Su Han , Pirmin A. Ulmann
IPC分类号： C12Q1/68 , G01N33/68
CPC分类号： G01N33/6815 , C12Q1/6825 , C12Q2563/155 , C12Q2527/125 , C12Q2527/107
摘要： The invention provides methods to detect cysteine which employ oligonucleotide functionalized nanoparticles.
摘要翻译：本发明提供了使用寡核苷酸官能化纳米颗粒检测半胱氨酸的方法。

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