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1. 发明申请

US20100062542A1 ANTIBODY AGAINST AFLATOXINS, SUPPORT USING THE ANTIBODY, METHOD OF IMMUNOLOGICALLY DETECTING AFLATOXINS AND METHOD OF CONCENTRATING AND PURIFYING AFLATOXINS 有权
标题翻译：抗AFLATOXINS的抗体，使用抗体的支持，免疫检测AFLATOXINS的方法和浓缩和净化AFLATOXINS的方法
公开(公告)号：US20100062542A1
公开(公告)日：2010-03-11
申请号：US12515263
申请日：2007-11-13
申请人： Mikiko Uchigashima , Shiro Miyake , Hiroshi Yamashita
发明人： Mikiko Uchigashima , Shiro Miyake , Hiroshi Yamashita
IPC分类号： C07K16/14 , B01D15/38 , C07D493/16 , C12N5/12 , G01N33/566 , G01N33/544
CPC分类号： G01N33/56961 , C07D493/14 , C07D493/22 , C07K16/14 , C07K16/44 , Y10S530/809
摘要： It is intended to detect, concentrate and purify aflatoxins of all types which are possibly contained in a sample such as a food. It is also intended to detect the total amount or the individual amounts thereof at a high sensitivity. By using aflatoxin B2 or its derivative as a hapten compound, an antibody, which shows the same reactivity to individual aflatoxin analogs and is highly tolerant to organic solvents, is obtained. Then, a detection/concentration/purification means and an immunological detection means with the use of the above antibody are constructed. The detection means thus constructed achieves a high sensitivity and excellent quantification properties.
摘要翻译：旨在检测，浓缩和纯化可能包含在诸如食物的样品中的所有类型的黄曲霉毒素。也用于以高灵敏度检测总量或其个体量。通过使用黄曲霉毒素B2或其衍生物作为半抗原化合物，获得对个体黄曲霉毒素类似物具有相同反应性并且对有机溶剂高度耐受的抗体。然后，构建使用上述抗体的检测/浓缩/纯化方法和免疫检测手段。这样构成的检测装置具有高灵敏度和极好的定量特性。

2. 发明授权

US08153767B2 Antibody against aflatoxins, support using the antibody, method of immunologically detecting aflatoxins and method of concentrating and purifying aflatoxins 有权
标题翻译：抗黄曲霉毒素抗体，使用抗体的支持，免疫学检测黄曲霉毒素的方法和浓缩和纯化黄曲霉毒素的方法
公开(公告)号：US08153767B2
公开(公告)日：2012-04-10
申请号：US12515263
申请日：2007-11-13
申请人： Mikiko Uchigashima , Shiro Miyake , Hiroshi Yamashita
发明人： Mikiko Uchigashima , Shiro Miyake , Hiroshi Yamashita
IPC分类号： C07K16/00 , C12P21/08
CPC分类号： G01N33/56961 , C07D493/14 , C07D493/22 , C07K16/14 , C07K16/44 , Y10S530/809
摘要： It is intended to detect, concentrate and purify aflatoxins of all types which are possibly contained in a sample such as a food. It is also intended to detect the total amount or the individual amounts thereof at a high sensitivity. By using aflatoxin B2 or its derivative as a hapten compound, an antibody, which shows the same reactivity to individual aflatoxin analogs and is highly tolerant to organic solvents, is obtained. Then, a detection/concentration/purification means and an immunological detection means with the use of the above antibody are constructed. The detection means thus constructed achieves a high sensitivity and excellent quantification properties.
摘要翻译：旨在检测，浓缩和纯化可能包含在诸如食物的样品中的所有类型的黄曲霉毒素。也用于以高灵敏度检测总量或其个体量。通过使用黄曲霉毒素B2或其衍生物作为半抗原化合物，获得对个体黄曲霉毒素类似物具有相同反应性并且对有机溶剂高度耐受的抗体。然后，构建使用上述抗体的检测/浓缩/纯化方法和免疫检测手段。这样构成的检测装置具有高灵敏度和极好的定量特性。

3. 发明申请

US20100047927A1 KIT FOR MEASUREMENT OF TERMITE INSECTICIDE ACTIVE INGREDIENT BY IMMUNOASSAY METHOD 有权
标题翻译：用免疫测定方法测定灭活素活性成分的试剂盒
公开(公告)号：US20100047927A1
公开(公告)日：2010-02-25
申请号：US12097627
申请日：2006-12-14
申请人： Shiro Miyake , Mikiko Uchigashima , Atsushi Kadowaki
发明人： Shiro Miyake , Mikiko Uchigashima , Atsushi Kadowaki
IPC分类号： G01N33/543 , G01N33/53 , G01N21/75
CPC分类号： G01N21/78 , G01N33/5308 , G01N2430/10
摘要： A kit and a method are provided for easily measuring the concentration of an active ingredient of a termite insecticide persisting in soil, particularly on site where a termite insecticide was actually applied. A kit of the present invention comprises 1) an extraction unit for extracting, with an solvent, a termite insecticide active ingredient from an object of measurement and 2) a reaction unit including a reaction container for encapsulating an identifying antigen, a fixing member for immobilizing an antibody against an active ingredient, and a sealing member capable of fitting to the reaction container. The kit optionally includes 3) a detection unit for visually or optically detecting a change depending on the concentrations of the active ingredient in the object of measurement, and 4) a dilution unit for diluting the sample solution to a certain ratio.
摘要翻译：提供了一种试剂盒和方法，用于容易地测量持续存在于土壤中的白蚁杀虫剂的活性成分的浓度，特别是在实际应用白蚁杀虫剂的地方。本发明的试剂盒包括：1）提取单元，用于从溶剂中提取由测量对象产生的白蚁杀虫剂活性成分; 2）包括用于封装识别抗原的反应容器的反应单元，用于固定的固定部件针对活性成分的抗体，以及能够与反应容器配合的密封构件。该试剂盒任选地包括3）用于根据测量对象中活性成分的浓度视觉或光学检测变化的检测单元，以及4）用于将样品溶液稀释至一定比例的稀释单元。

4. 发明授权

US08323904B2 Kit for measurement of termite insecticide active ingredient by immunoassay method 有权
标题翻译：通过免疫测定法测定白蚁杀虫剂活性成分的试剂盒
公开(公告)号：US08323904B2
公开(公告)日：2012-12-04
申请号：US12097627
申请日：2006-12-14
申请人： Shiro Miyake , Mikiko Uchigashima , Atsushi Kadowaki
发明人： Shiro Miyake , Mikiko Uchigashima , Atsushi Kadowaki
IPC分类号： G01N33/53
CPC分类号： G01N21/78 , G01N33/5308 , G01N2430/10
摘要： A kit and a method are provided for easily measuring the concentration of an active ingredient of a termite insecticide persisting in soil, particularly on site where a termite insecticide was actually applied. A kit of the present invention comprises 1) an extraction unit for extracting, with an solvent, a termite insecticide active ingredient from an object of measurement and 2) a reaction unit including a reaction container for encapsulating an identifying antigen, a fixing member for immobilizing an antibody against an active ingredient, and a sealing member capable of fitting to the reaction container. The kit optionally includes 3) a detection unit for visually or optically detecting a change depending on the concentrations of the active ingredient in the object of measurement, and 4) a dilution unit for diluting the sample solution to a certain ratio.
摘要翻译：提供了一种试剂盒和方法，用于容易地测量持续存在于土壤中的白蚁杀虫剂的活性成分的浓度，特别是在实际应用白蚁杀虫剂的地方。本发明的试剂盒包括：1）提取单元，用于从溶剂中提取由测量对象产生的白蚁杀虫剂活性成分; 2）包括用于封装识别抗原的反应容器的反应单元，用于固定的固定部件针对活性成分的抗体，以及能够与反应容器配合的密封构件。该试剂盒任选地包括3）用于根据测量对象中活性成分的浓度视觉或光学检测变化的检测单元，以及4）用于将样品溶液稀释至一定比例的稀释单元。

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