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1. 发明授权

US07592160B2 Processes for producing isomaltose and isomaltitol and use thereof 有权
标题翻译：制备异麦芽糖和异麦芽糖醇的方法及其用途
公开(公告)号：US07592160B2
公开(公告)日：2009-09-22
申请号：US10492932
申请日：2002-10-18
申请人： Michio Kubota , Tomoyuki Nishimoto , Tomohiko Sonoda , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Tomoyuki Nishimoto , Tomohiko Sonoda , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/44
CPC分类号： C12P19/12
摘要： The present invention aims to provide a novel process for producing isomaltose and isomaltitol, and uses thereof, and it solves the object by establishing a process for producing isomaltose comprising a step of contacting a saccharide, having the α-1,4 glucosidic linkage as the linkage of non-reducing end and a glucose polymerization degree of at least two, with an α-isomaltosyl-transferring enzyme and an α-isomaltosylglucosaccharide-forming enzyme derived from a specific microorganism; a process for producing isomaltitol using the isomaltose produced by the above process; saccharide compositions comprising the isomaltose and/or the isomaltitol produced by the above processes; and uses thereof.
摘要翻译：本发明的目的在于提供一种制备异麦芽糖和异麦芽糖醇的新方法及其用途，其通过建立异麦芽糖的制备方法来解决该问题，该方法包括使具有α-1,4糖苷键的糖类作为非还原末端与葡萄糖聚合度至少为2的α-异麦芽糖基转移酶和源自特异性微生物的α-异麦芽糖基葡萄糖形成酶的连接; 使用由上述方法生产的异麦芽糖制备异麦芽糖醇的方法; 包含通过上述方法生产的异麦芽糖和/或异麦芽糖醇的糖组合物; 及其用途。

2. 发明申请

US20100285562A1 ISOCYCLOMALTOOLIGOSACCHARIDE(S), ISOCYCLOMALTOOLIGOSACCHARIDE-FORMING ENZYME, THEIR PREPARATION AND USES 有权
标题翻译：异羟肟酸（SOCYCLOMALTOOLIGOSACCHARIDE）（S），异羟肟酸吡喃葡萄糖苷形成酶，其制备和用途
公开(公告)号：US20100285562A1
公开(公告)日：2010-11-11
申请号：US12764927
申请日：2010-04-21
申请人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12N9/00 , C12N1/20 , C07H21/04 , C12N15/70 , C12N1/21
CPC分类号： C07H3/06 , A23L29/35 , A23L33/25 , C12N9/2402 , C12N9/2405 , C12P19/04
摘要： A non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents, a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof are provided by use of an isocyclomaltooligosaccharide(s) having a structure represented by Formula 1, Cyclo{→6)-[α-D-Glcp-(1→4)]n-α-D-Glcp-(1→}, wherein “n” is 4 or 5.
摘要翻译：通过提供由葡萄糖组成的新型非还原性糖类，形成非还原性糖类的新型酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体，得到非还原性糖类，该DNA是含有非还原性糖的组合物及其用途，通过使用具有式1所示结构的环状环糊精寡糖（Cyclo（→6）） - [α-D-Glcp-（1→4））] n-α-D-Glcp-（1→}，其中“n”为4或5。

3. 发明申请

US20100015671A1 CYCLIC MALTOSYLMALTOSE, CYCLIC MALTOSYMALTOSE-FORMING ENZYME, THEIR PREPARATION AND USES 有权
标题翻译：环状麦芽糖醇，环状麦芽糖醇形成酶，其制备和用途
公开(公告)号：US20100015671A1
公开(公告)日：2010-01-21
申请号：US11941862
申请日：2007-11-16
申请人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/18 , C12N9/10
CPC分类号： A61K9/2018 , A21D2/181 , A23L2/52 , A23L2/60 , A23L13/428 , A23L27/33 , A23L33/10 , A61K8/60 , A61Q11/00 , C07H3/06 , C12N9/1048 , C12P19/18
摘要： An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.
摘要翻译：本发明的目的是通过提供一种由葡萄糖组成的新型非还原性糖组成并提供形成非还原性糖的新型酶，提供一种非还原性糖的方法及其制备方法，编码该酶的DNA，重组DNA和包含该DNA的转化体，包含非还原性糖的组合物及其用途。本发明通过提供具有环{> 6）-α-D-吡喃葡萄糖基 - （1,4）-alpha-D-吡喃葡萄糖基 - （1,6）-alpha-D结构的新型环状糖来解决上述目的 - 吡喃葡萄糖基 - （1,4）-alpha-D-吡喃葡萄糖基 - （1>），环状麦芽糖基麦芽糖，新的环状麦芽糖基麦芽糖酶形成酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体包含该DNA，包含环状麦芽糖麦芽糖的组合物或包含其的糖组合物及其用途。

4. 发明授权

US07186701B2 Dehydrating agent and method for dehydrating moist article using the agent and dehydrated article obtained by the method 有权
标题翻译：使用该方法获得的试剂和脱水制品脱水剂和脱水湿物品的方法
公开(公告)号：US07186701B2
公开(公告)日：2007-03-06
申请号：US10466438
申请日：2002-01-17
申请人： Michio Kubota , Tomoyuki Nishimoto , Hajime Aga , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Tomoyuki Nishimoto , Hajime Aga , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A61K31/702 , C08B37/00
CPC分类号： B01J20/262 , A21D2/181 , A23C13/125 , A23L3/42 , A61K9/0014 , A61K47/26 , A61K47/40 , B01J20/22 , C07H3/06
摘要： The present invention has the object to dehydrate hydrous matters without denaturing or deteriorating them by using a dehydrating agent comprising an anhydrous cyclotetrasaccharide, and provides a dehydrating agent comprising the cyclotetrasaccharide; a method for dehydrating hydrous matters through a step of incorporating, contacting or coexisting the cyclotetrasaccharide into, with, or in the hydrous matters; and dehydrated products obtainable thereby.
摘要翻译：本发明的目的是通过使用包含无水环四糖的脱水剂使含水物脱水而不使其变性或变质，并提供包含环四糖的脱水剂; 通过将环四糖与含水物质结合，接触或共存的步骤脱水含水物质的方法; 和可得到的脱水产品。

5. 发明申请

US20070154996A1 Method for transferring a glucosyl residue 审中-公开
标题翻译：转移葡糖基残基的方法
公开(公告)号：US20070154996A1
公开(公告)日：2007-07-05
申请号：US10587711
申请日：2005-01-27
申请人： Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/04
CPC分类号： C12P19/18 , C12P19/44
摘要： An object of the present invention is to provide novel methods for forming glucosyl-transferred polyalcohols, glucosyl-transferred glucuronic acid, and glucosyl-transferred derivatives of glucose whose C-6 hydroxyl group bound to a saccharide by using an enzymatic reaction. The present invention solves the above object by providing a method for transferring a glucosyl residue to polyalcohols, glucuronic acid and/or derivatives of glucose whose C-6 hydroxyl group bound to a saccharide, comprising a step of: allowing a trehalose phosphorylase to act on a saccharide containing glucose as a component sugar and one or more polyalcohols selected from the group consisting of inositol, ribitol, erythritol, and glycerol; glucuronic acid and/or a salt thereof; and/or one or more derivatives of glucose whose C-6 hydroxyl group bound to a saccharide selected from the group consisting of isomaltose, gentiobiose, melibiose, isomaltotriose, and isopanose.
摘要翻译：本发明的目的是提供通过酶促反应形成C-6羟基与糖结合的葡萄糖转移的多元醇，葡糖基转移的葡萄糖醛酸和葡萄糖转移的葡萄糖转移衍生物的新方法。本发明通过提供一种将葡糖基残基转移到多糖，葡糖醛酸和/或其C-6羟基与糖结合的葡萄糖的衍生物的方法，包括以下步骤：使海藻糖磷酸化酶作用于含有葡萄糖作为组分糖和一种或多种选自肌醇，核糖醇，赤藓糖醇和甘油的多元醇的糖; 葡萄糖醛酸和/或其盐; 和/或一种或多种C-6羟基结合于选自异麦芽糖，龙胆糖，蜜二糖，异麦芽三糖和异丙糖的糖的葡萄糖衍生物。

6. 发明授权

US07192746B2 α-Isomaltosyltransferase, process for producing the same and use thereof 有权
标题翻译： α-异麦芽糖基转移酶，其制备方法和用途
公开(公告)号：US07192746B2
公开(公告)日：2007-03-20
申请号：US10296153
申请日：2001-05-22
申请人： Michio Kubota , Tomoyuki Nishimoto , Hajime Aga , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Tomoyuki Nishimoto , Hajime Aga , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/18 , C12N9/10 , C12N1/20
CPC分类号： A23G1/423 , A21D2/181 , A23C9/005 , A23C9/1307 , A23G1/40 , A23G1/56 , A23G3/346 , A23G4/10 , A23G2200/06 , A23L2/60 , A23L9/12 , A23L11/07 , A23L13/428 , A23L15/30 , A23L19/01 , A23L27/33 , A23L29/35 , A61K8/60 , A61K8/66 , A61Q11/00 , A61Q15/00 , A61Q17/04 , A61Q19/00 , A61Q19/02 , A61Q19/08 , A61Q19/10 , C07H3/06 , C12N9/1048 , C12P19/18
摘要： The object of the present invention is to provide an α-isomaltosyl-transferring enzyme which forms a cyclotetrasaccharide having the structure of cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→} from a saccharide having a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end; microorganisms which produce the enzyme; process for producing the enzyme; cyclotetrasaccharide or saccharide compositions comprising the same; and uses thereof.
摘要翻译：本发明的目的是提供一种α-异麦芽糖基转移酶，其形成具有环{ - > 6）-al-D-glucopyranosyl-（1-> 3）-al-D-glucopyranosyl- （1-> 6）-α-D-吡喃葡萄糖基 - （1-> 3）-alpha-D-吡喃葡萄糖基 - （1->}，其葡萄糖聚合度至少为3，并具有α-1 ，6个糖苷键作为非还原末端的连接键和除非还原末端之间的连接以外的α-1,4糖苷键;产生该酶的微生物;产生该酶的方法;环四糖或糖组合物，其包含相同;及其用途。

7. 发明授权

US07709230B2 Process for producing isomaltose and uses thereof 有权
标题翻译：异麦芽糖的制备方法及其用途
公开(公告)号：US07709230B2
公开(公告)日：2010-05-04
申请号：US11777044
申请日：2007-07-12
申请人： Michio Kubota , Tomoyuki Nishimoto , Takanobu Higashiyama , Hikaru Watanabe , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Tomoyuki Nishimoto , Takanobu Higashiyama , Hikaru Watanabe , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/44 , C12P19/18 , C12P19/04 , C12N9/00 , C12N9/10 , C12P21/06 , C07H1/00
CPC分类号： C12P19/12
摘要： A novel process for producing isomaltose and uses thereof and comprising the steps of contacting saccharides, which have a glucose polymerization degree of at least two and α-1,4 glucosidic linkage as a linkage at the non-reducing end, with an α-isomaltosylglucosaccharide-forming enzyme, in the presence or the absence of α-isomaltosyl-transferring enzyme to form a-isomaltosylglucosaccharides, which have a glucose polymerization degree of at least three, α-1,6 glucosidic linkage as a linkage at the non-reducing end, and α-1,4 glucosidic linkage as a linkage other than the non-reducing end, and/or to form cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→}; contacting the saccharides so formed with isomaltose-releasing enzyme to release isomaltose; and collecting the released isomaltose; and uses thereof.
摘要翻译：一种生产异麦芽糖的新方法及其应用，包括以下步骤：将非还原末端具有至少两个葡萄糖聚合度和至少两个α-1,4糖苷键作为键的糖与α-异麦芽糖基葡萄糖在α-异麦芽糖基转移酶的存在或不存在下形成α-异麦芽糖基葡糖，其在非还原末端具有至少三个α-1,6糖苷键作为键的葡萄糖聚合度和α-1,4糖苷键作为非还原端以外的键，和/或形成环{→6）-α-D-吡喃葡萄糖基 - （1→3）-α-D-吡喃葡萄糖基 - （ 1→6）-α-D-吡喃葡萄糖基 - （1→3）-α-D-吡喃葡萄糖基 - （1→};使与异麦芽糖释放酶形成的糖接触释放异麦芽糖;收集释放的异麦芽糖; 其中。

8. 发明申请

US20060008791A1 Dehydrating agent and method for dehydrating moist article using the agent and dehydrated article obtained by the method 有权
标题翻译：使用该方法获得的试剂和脱水制品脱水剂和脱水湿物品的方法
公开(公告)号：US20060008791A1
公开(公告)日：2006-01-12
申请号：US10466438
申请日：2002-01-17
申请人： Michio Kubota , Tomoyuki Nishimoto , Hajime Aga , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Tomoyuki Nishimoto , Hajime Aga , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A01N1/02 , B01J20/00
CPC分类号： B01J20/262 , A21D2/181 , A23C13/125 , A23L3/42 , A61K9/0014 , A61K47/26 , A61K47/40 , B01J20/22 , C07H3/06
摘要： The present invention has the object to dehydrate hydrous matters without denaturing or deteriorating them by using a dehydrating agent comprising an anhydrous cyclotetrasaccharide, and provides a dehydrating agent comprising the cyclotetrasaccharide; a method for dehydrating hydrous matters through a step of incorporating, contacting or coexisting the cyclotetrasaccharide into, with, or in the hydrous matters; and dehydrated products obtainable thereby.
摘要翻译：本发明的目的是通过使用包含无水环四糖的脱水剂使含水物脱水而不使其变性或变质，并提供包含环四糖的脱水剂; 通过将环四糖与含水物质结合，接触或共存的步骤脱水含水物质的方法; 和可得到的脱水产品。

9. 发明授权

US07732426B2 Isocyclomaltooligosaccharide (S) , isocyclomaltooligosaccharide-forming enzyme, their preparation and uses 有权
标题翻译：异环寡糖（S），异环寡糖寡糖形成酶，其制备和用途
公开(公告)号：US07732426B2
公开(公告)日：2010-06-08
申请号：US11663919
申请日：2005-09-26
申请人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A61K31/715 , A61K31/702 , C07H1/00
CPC分类号： C07H3/06 , A23L29/35 , A23L33/25 , C12N9/2402 , C12N9/2405 , C12P19/04
摘要： The present invention have objects to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above objects by providing an isocyclomaltooligosaccharide(s) having a structure represented by General Formula 1, a novel isocyclomaltooligosaccharide-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the isocyclomaltooligosaccharide(s) or a saccharide composition comprising the same, and uses thereof. Cyclo{→6)-[α-D-Glcp-(1→4)]n-α-D-Glcp-(1→} General Formula 1 (In General Formula 1, “n” means a number of 4 or 5).
摘要翻译：本发明的目的是提供一种由葡萄糖组成的新型非还原性糖组成的非还原性糖类，提供形成非还原性糖类的新型酶，其制造方法及其制造方法，编码酶的DNA，重组DNA和包含DNA的转化体，包含非还原性糖的组合物及其用途。本发明通过提供具有由通式1表示的结构的异环型低聚半乳糖，新型的异环型低聚葡萄糖形成酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体来解决上述目的包含DNA，包含异环寡脱醇糖的组合物或包含该组合物的糖组合物及其用途。环状{→6） - [α-D-Glcp-（1→4）] n-α-D-Glcp-（1→}通式1（在通式1中，“n”表示4或5 ）。

10. 发明授权

US07309697B2 Cyclic maltosylmatose 有权
标题翻译：循环麦芽糖
公开(公告)号：US07309697B2
公开(公告)日：2007-12-18
申请号：US10569959
申请日：2004-08-26
申请人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A61K31/715 , C07H1/00
CPC分类号： A61K9/2018 , A21D2/181 , A23L2/52 , A23L2/60 , A23L13/428 , A23L27/33 , A23L33/10 , A61K8/60 , A61Q11/00 , C07H3/06 , C12N9/1048 , C12P19/18
摘要： An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{→6)-α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→6}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.
摘要翻译：本发明的目的是通过提供一种由葡萄糖组成的新型非还原性糖组成并提供形成非还原性糖的新型酶，提供一种非还原性糖的方法及其制备方法，编码该酶的DNA，重组DNA和包含该DNA的转化体，包含非还原性糖的组合物及其用途。本发明通过提供具有环{ - > 6）-α-D-吡喃葡萄糖基 - （1-> 4）-α-D-吡喃葡萄糖基 - （1-> 6） - 结构的新型环状糖来解决上述目的。 α-D-吡喃葡萄糖基 - （1-> 4）-α-D-吡喃葡萄糖基 - （1-> 6），环状麦芽糖基麦芽糖，新型环状麦芽糖基麦芽糖酶形成酶，其制备方法和方法，编码酶，重组DNA和包含该DNA的转化体，包含环状麦芽糖基麦芽糖的组合物或包含其的糖组合物及其用途。

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