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    • 6. 发明授权
    • Flow cytometer for classifying leukocytes and method for determining detection angle range of the same
    • 用于分类白细胞的流式细胞仪和确定其检测角度范围的方法
    • US07092078B2
    • 2006-08-15
    • US10402133
    • 2003-03-31
    • Yutaka NagaiKazuo YamagishiTomonobu Maruyama
    • Yutaka NagaiKazuo YamagishiTomonobu Maruyama
    • G01N33/48
    • G01N21/532G01N15/1459G01N33/56972G01N2015/008G01N2015/1493
    • A blood sample containing leukocytes flows through a flow cell. A light source emits a light beam in a first direction. The light beam incident into the flow cell is scattered by the blood sample as scattered light. A first detector detects an intensity of forward small scattered light out of the scattered light. The first detector is arranged so as to have a maximum angle of a detection angle range for the forward small scattered light stemmed from the first direction at a position inside (outside) of the flow cell, which is 3.1 (4) degrees or less. A second detector detects an intensity of forward large scattered light out of the scattered light. The second detector is arranged so as to have a detection angle range for the forward large scattered light stemmed from the first direction at a position inside (outside) of the flow cell, which falls in a range from 8 to 12 (10 to 16) degrees. A processor classifies the leukocytes based on the detected intensity of the forward large scattered light and the detected intensity of the forward small scattered light.
    • 含有白细胞的血液样品流过流动池。 光源沿第一方向发射光束。 入射到流动池中的光束作为散射光被血样散射。 第一检测器检测散射光中的前向小散射光的强度。 第一检测器被布置成在流动池的内部(外部)的位置处具有从第一方向起始的前向小散射光的检测角范围的最大角度为3.1(4)度以下。 第二检测器检测散射光中的前向大散射光的强度。 第二检测器被布置成具有从流动池的内部(外部)的位置处的从第一方向起始的向前大的散射光的检测角度范围,该范围在8〜12(10〜16)的范围内, 度。 处理器基于前向大散射光的检测强度和前向小散射光的检测强度对白细胞进行分类。
    • 7. 发明申请
    • Flow cytometer for classifying leukocytes and method for determining detection angle range of the same
    • 用于分类白细胞的流式细胞仪和确定其检测角度范围的方法
    • US20050219508A1
    • 2005-10-06
    • US11135364
    • 2005-05-24
    • Yutaka NagaiKazuo YamagishiTomonobu Maruyama
    • Yutaka NagaiKazuo YamagishiTomonobu Maruyama
    • G01N15/00G01N15/14G01N21/53G01N33/569G01N33/48
    • G01N21/532G01N15/1459G01N33/56972G01N2015/008G01N2015/1493
    • A blood sample containing leukocytes flows through a flow cell. A light source emits a light beam in a first direction. The light beam incident into the flow cell is scattered by the blood sample as scattered light. A first detector detects an intensity of forward small scattered light out of the scattered light. The first detector is arranged so as to have a maximum angle of a detection angle range for the forward small scattered light stemmed from the first direction at a position inside (outside) of the flow cell, which is 3.1 (4) degrees or less. A second detector detects an intensity of forward large scattered light out of the scattered light. The second detector is arranged so as to have a detection angle range for the forward large scattered light stemmed from the first direction at a position inside (outside) of the flow cell, which falls in a range from 8 to 12 (10 to 16) degrees. A processor classifies the leukocytes based on the detected intensity of the forward large scattered light and the detected intensity of the forward small scattered light.
    • 含有白细胞的血液样品流过流动池。 光源沿第一方向发射光束。 入射到流动池中的光束作为散射光被血样散射。 第一检测器检测散射光中的前向小散射光的强度。 第一检测器被布置成在流动池的内部(外部)的位置处具有从第一方向起始的前向小散射光的检测角范围的最大角度为3.1(4)度以下。 第二检测器检测散射光中的前向大散射光的强度。 第二检测器被布置成具有从流动池的内部(外部)的位置处的从第一方向起始的向前大的散射光的检测角度范围,该范围在8〜12(10〜16)的范围内, 度。 处理器基于前向大散射光的检测强度和前向小散射光的检测强度对白细胞进行分类。
    • 10. 发明授权
    • Flow cytometer for classifying leukocytes and method for determining detection angle range of the same
    • 用于分类白细胞的流式细胞仪和确定其检测角度范围的方法
    • US07369231B2
    • 2008-05-06
    • US11135364
    • 2005-05-24
    • Yutaka NagaiKazuo YamagishiTomonobu Maruyama
    • Yutaka NagaiKazuo YamagishiTomonobu Maruyama
    • G01N21/00
    • G01N21/532G01N15/1459G01N33/56972G01N2015/008G01N2015/1493
    • A blood sample containing leukocytes flows through a flow cell. A light source emits a light beam in a first direction. The light beam incident into the flow cell is scattered by the blood sample as scattered light. A first detector detects an intensity of forward small scattered light out of the scattered light. The first detector is arranged so as to have a maximum angle of a detection angle range for the forward small scattered light stemmed from the first direction at a position inside (outside) of the flow cell, which is 3.1 (4) degrees or less. A second detector detects an intensity of forward large scattered light out of the scattered light. The second detector is arranged so as to have a detection angle range for the forward large scattered light stemmed from the first direction at a position inside (outside) of the flow cell, which falls in a range from 8 to 12 (10 to 16) degrees. A processor classifies the leukocytes based on the detected intensity of the forward large scattered light and the detected intensity of the forward small scattered light.
    • 含有白细胞的血液样品流过流动池。 光源沿第一方向发射光束。 入射到流动池中的光束作为散射光被血样散射。 第一检测器检测散射光中的前向小散射光的强度。 第一检测器被布置成在流动池的内部(外部)的位置处具有从第一方向起始的前向小散射光的检测角范围的最大角度为3.1(4)度以下。 第二检测器检测散射光中的前向大散射光的强度。 第二检测器被布置成具有从流动池的内部(外部)的位置处的从第一方向起始的向前大的散射光的检测角度范围,该范围在8〜12(10〜16)的范围内, 度。 处理器基于前向大散射光的检测强度和前向小散射光的检测强度对白细胞进行分类。