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1. 发明授权

US5196314A Process and reagent for the determination of substrates or enzyme activities 失效
标题翻译：用于测定底物或酶活性的方法和试剂
公开(公告)号：US5196314A
公开(公告)日：1993-03-23
申请号：US661271
申请日：1991-02-26
申请人： Michael-Harold Town , Joachim Siedel , Joachim Ziegenhorn
发明人： Michael-Harold Town , Joachim Siedel , Joachim Ziegenhorn
IPC分类号： C07D257/04 , C12Q1/26 , C12Q1/28
CPC分类号： C07D257/04 , C12Q1/26 , C12Q1/28 , C12Q2326/92 , Y10S435/805
摘要： A process for the determination of substrate or enzyme activities by the use of a redox reaction as a measurement reaction is carried out in the presence of one or more additionally added tetrazolium salts to remove disturbing substances. The tetrazolium salts have the formula ##STR1## in which R.sup.1 is a hydrogen atom, a carboxyl group or an alkyl, phenyl, nitrophenyl, dinitrophenyl, carboxyl-substituted phenyl or trialkylammoniumphenyl radical, R.sup.2 is a phenyl, nitrophenyl, biphenylyl or naphthyl radical, R.sup.3 is a phenyl, carboxyl-substituted phenyl, carboxyl-substituted hydroxyphenyl or dimethylthiazolyl radical, and A.sup..crclbar. is a monovalent anion. The formazanes formed by reaction with reducing substances do not absorb light at all, or absorb light only to a negligible extent, at the measurement wavelength of the redox reaction.New compounds included within the structural formula are those in which R.sup.1 is carboxy, phenyl, 2-carboxyphenyl, 4-carboxyphenyl, 2,4-dinitrophenyl or 4-trimethylammoniumphenyl, R.sup.2 is phenyl, and R.sup.3 is phenyl or 2-carboxyphenyl; with the proviso that, when R.sup.3 is unsubstituted phenyl or 2-carboxyphenyl, R.sup.1 may not be unsubstituted phenyl.
摘要翻译：通过使用氧化还原反应作为测量反应来测定底物或酶活性的方法在一种或多种另外加入的四唑鎓盐的存在下进行以除去扰乱物质。所述四唑鎓盐具有其中R 1为氢原子，羧基或烷基，苯基，硝基苯基，二硝基苯基，羧基取代的苯基或三烷基铵苯基的式（I），R 2为苯基，硝基苯基，联苯基或萘基，R3是苯基，羧基取代的苯基，羧基取代的羟基苯基或二甲基噻唑基，A（ - ）是一价阴离子。通过与还原物质反应而形成的甲烷在氧化还原反应的测定波长下根本不吸收光，或仅吸收光可忽略的程度。包括在结构式中的新化合物是其中R1是羧基，苯基，2-羧基苯基，4-羧基苯基，2,4-二硝基苯基或4-三甲基铵苯基，R2是苯基，R3是苯基或2-羧基苯基的化合物; 条件是当R3是未取代的苯基或2-羧基苯基时，R 1可以不是未被取代的苯基。

2. 发明授权

US5013647A Removal of interfering reducing substances with tetrazolium salts in redox measurement reactions 失效
标题翻译：在氧化还原测定反应中用四唑盐除去干扰还原物质
公开(公告)号：US5013647A
公开(公告)日：1991-05-07
申请号：US35617
申请日：1987-04-03
申请人： Michael-Harold Town , Joachim Siedel , Joachim Ziegenhorn
发明人： Michael-Harold Town , Joachim Siedel , Joachim Ziegenhorn
IPC分类号： C07D257/04 , C12Q1/26 , C12Q1/28 , G01N33/50
CPC分类号： C07D257/04 , C12Q1/26 , C12Q1/28 , C12Q2326/92 , Y10S435/805
摘要： A process for the determination of substrate or enzyme activities by the use of a redox reaction as a measurement reaction is carried out in the presence of one or more additionally added tetrazolium salts to remove disturbing substances. The tetrazolium salts have the formula ##STR1## in which R.sup.1 is a hydrogen atom, a carboxyl group or an alkyl, phenyl, nitrophenyl, dinitrophenyl, carboxyl-substituted phenyl or trialkylammoniumphenyl radical, R.sup.2 is a phenyl, nitrophenyl, biphenylyl or naphthyl radical, R.sup.3 is a phenyl, carboxyl-substituted phenyl, carboxyl-substituted hydroxyphenyl or dimethylthiazolyl radical, and A.sup..crclbar. is a monovalent anion. The formazanes formed by reaction with reducing substances do not absorb light at all, or absorb light only to a negligible extent, at the measurement wavelength of the redox reaction. New compounds included within the formula are those in which R.sup.1 is carboxy, phenyl, 2-carboxy, phenyl, 2-carboxyphenyl, 4-carboxyphenyl, 2,4-dinitrophenyl or 4-trimethylammoniumphenyl, R.sup.2 is phenyl, and R.sup.3 is phenyl or 2-carboxyphenyl; with the proviso that, when R.sup.3 is unsubstituted phenyl or 2-carboxyphenyl, R.sup.1 may not be unsubstituted phenyl.
摘要翻译：通过使用氧化还原反应作为测量反应来测定底物或酶活性的方法在一种或多种另外加入的四唑鎓盐的存在下进行以除去扰乱物质。所述四唑鎓盐具有其中R 1为氢原子，羧基或烷基，苯基，硝基苯基，二硝基苯基，羧基取代的苯基或三烷基铵苯基的式（I），R 2为苯基，硝基苯基，联苯基或萘基，R3是苯基，羧基取代的苯基，羧基取代的羟基苯基或二甲基噻唑基，A（ - ）是一价阴离子。通过与还原物质反应而形成的甲烷在氧化还原反应的测定波长下根本不吸收光，或仅吸收光可忽略的程度。包括在式中的新化合物是其中R1是羧基，苯基，2-羧基，苯基，2-羧基苯基，4-羧基苯基，2,4-二硝基苯基或4-三甲基铵苯基，R2是苯基，R3是苯基或2 -carboxyphenyl; 条件是当R3是未取代的苯基或2-羧基苯基时，R 1可以不是未被取代的苯基。

3. 发明授权

US4645739A Process and reagent for the determination of N-carbamoylsarcosine with the use of a new enzyme 失效
标题翻译：使用新酶测定N-氨基甲酰肌氨酸的方法和试剂
公开(公告)号：US4645739A
公开(公告)日：1987-02-24
申请号：US562072
申请日：1983-12-16
申请人： Rolf Deeg , Albert Roder , Joachim Siedel , Helmgard Gauhl , Joachim Ziegenhorn
发明人： Rolf Deeg , Albert Roder , Joachim Siedel , Helmgard Gauhl , Joachim Ziegenhorn
IPC分类号： C12N9/80 , C12Q1/34 , C12R1/01 , C12R1/06 , C12R1/265 , G01N33/70 , C12Q1/26 , C12Q1/00 , C12Q1/28
CPC分类号： G01N33/70 , C12N9/80 , C12Q1/34 , G01N2333/98 , Y10S435/81 , Y10S435/814 , Y10S435/815 , Y10S435/816 , Y10S435/822 , Y10S435/83 , Y10S435/859
摘要： The present invention provides a process for the determination of N-carbamoylsarcosine, wherein a sample solution containing N-carbamoylsarcosine is reacted with N-carbamoylsarcosine-amidohydrolase to give sarcosine, which is then determined.The present invention also provides the enzyme N-carbamoylsarcosine-amidohydrolase, a process for obtaining it and a reagent containing it.
摘要翻译：本发明提供了测定N-氨基甲酰基肌氨酸的方法，其中将含有N-氨基甲酰基肌氨酸的样品溶液与N-氨基甲酰基肌氨酸 - 酰胺水解酶反应，得到肌氨酸，然后测定。本发明还提供了N-氨基甲酰基肌氨酸 - 酰氨基水解酶，其获得方法和含有它的试剂。

4. 发明授权

US4579825A Process for the complete and rapid removal of turbidity in a biological fluid 失效
标题翻译：在生物流体中完全快速除去浊度的过程
公开(公告)号：US4579825A
公开(公告)日：1986-04-01
申请号：US626633
申请日：1984-07-02
申请人： Joachim Siedel , Johnny Staepels , Joachim Ziegenhorn
发明人： Joachim Siedel , Johnny Staepels , Joachim Ziegenhorn
IPC分类号： G01N33/48 , B01F17/02 , B01F17/34 , G01N33/53 , G01N33/536 , G01N33/92 , G01N1/28
CPC分类号： G01N33/92 , Y10T436/104165 , Y10T436/107497 , Y10T436/25125
摘要： The present invention provides a process for the removal of turbidities in biological fluids by the addition of surface-active agents, wherein, as surface-active agents, there are used(a) a polyethoxylated triglyceride with an HLB value of 4 to 14,(b) a secondary n-alkane sulphonate, as well as optionally(c) a further non- or anionic tenside,in aqueous, optionally buffered solution.
摘要翻译：本发明提供通过添加表面活性剂来除去生物流体中的混浊的方法，其中作为表面活性剂，使用（a）HLB值为4至14的聚乙氧基化甘油三酸酯（ b）次级正烷烃磺酸盐，以及任选（c）另外的非水性或阴离子表面活性剂，在水性，任选缓冲溶液中。

5. 发明授权

US5288606A Reagent for specific determination of fructosamine 失效
标题翻译：具体测定果糖胺的试剂
公开(公告)号：US5288606A
公开(公告)日：1994-02-22
申请号：US911282
申请日：1992-07-09
申请人： Joachim Siedel , Joachim Ziegenhorn , Lieselotte Schellong , Bernd Vogt
发明人： Joachim Siedel , Joachim Ziegenhorn , Lieselotte Schellong , Bernd Vogt
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/30 , C12Q1/44 , C12Q1/54 , G01N33/66 , C12Q1/00 , A61K35/78 , A61K37/02 , C12P19/38
CPC分类号： C12Q1/26 , G01N33/66 , Y10S436/825 , Y10S436/904 , Y10T436/144444 , Y10T436/25125
摘要： The present invention provides a process for the specific determination of the serum fructosamine content in blood or samples derived from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, wherein, before the color reaction, non-specific reducing-acting and/or turbidity-causing sample components are removed at approximately neutral pH value, subsequently the pH is adjusted to a value of from 10 to 12 and the color reagent is added thereto.The present invention also provides a reagent mixture for the specific determination of the serum fructosamine content in blood or samples derived from blood, wherein it comprises a reagent for the removal of non-specific reducing-acting and/or turbidity-causing sample components, a rebuffering reagent with a buffer which has a pH value in the range of from 10.5 to 12.5 and a color reagent for the detection of fructosamine.
摘要翻译：本发明提供一种用于通过与适当的着色试剂反应并测量由此引起的颜色变化来特异性测定血液或血液中衍生血液的血清果糖胺含量的方法，其中在显色反应之前，非特异性还原在大约中性pH值下去除起泡和/或浊度的样品组分，随后将pH调节至10至12的值，并向其中加入着色剂。本发明还提供了用于特异性测定血液或血液中衍生血液中的果糖胺含量的试剂混合物，其中它包括用于除去非特异性还原作用和/或引起浊度的样品组分的试剂，具有pH值在10.5〜12.5范围内的缓冲液和用于检测果糖胺的显色试剂的缓冲试剂。

6. 发明授权

US4816393A Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof 失效
标题翻译：核苷三磷酸依赖性1-甲基乙内酰脲酶，其获得方法及其用途
公开(公告)号：US4816393A
公开(公告)日：1989-03-28
申请号：US704712
申请日：1985-02-25
申请人： Joachim Siedel , Rolf Deeg , Albert Roder , Joachim Ziegenhorn , Hans Mollering , Helmgard Gauhl
发明人： Joachim Siedel , Rolf Deeg , Albert Roder , Joachim Ziegenhorn , Hans Mollering , Helmgard Gauhl
IPC分类号： C12N9/14 , C12N9/86 , C12Q1/34 , C12R1/01 , C12R1/06 , C12R1/13 , C12R1/265 , G01N33/70 , C12Q1/26 , C12Q1/32
CPC分类号： C12N9/86 , C12Q1/34 , G01N33/70 , G01N2333/986 , Y10S435/81
摘要： The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions.The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it.Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination(a) of the hydrolysis product formed from 1-methylhydantoin with N-carbamoylsarcosinamidohydrolase with formation of sarcosine and detection of the sarcosine with sarcosine oxidase or sarcosine dehydrogenase or(b) of the simultaneously formed nucleoside diphosphate.
摘要翻译：本发明提供1-甲基乙内酰脲酶，其在核苷三磷酸和多价金属离子的存在下水解1-甲基乙内酰脲。本发明还提供了获得1-甲基乙内酰脲酶和含有它的试剂的方法。此外，本发明提供了通过将肌酐与肌酐去酰亚胺酶（EC 3.5.4.21）转化为1-甲基乙内酰脲来测定肌酸酐的方法，后者在核苷三磷酸存在下用1-甲基乙内酰脲酶水解，测定（a）由N-甲基乙内酰脲与N-氨基甲酰基肌氨酰氨基水解酶形成的水解产物（肌氨酸形成），并用肌氨酸氧化酶或肌氨酸脱氢酶检测肌氨酸，或（b）同时形成的二磷酸核苷。

7. 发明授权

US4578361A Creatinine antibody 失效
标题翻译：肌酸酐抗体
公开(公告)号：US4578361A
公开(公告)日：1986-03-25
申请号：US656957
申请日：1984-10-02
申请人： Joachim Siedel , Ulrich Neumann , Joachim Ziegenhorn , Hans-Georg Batz , Helmut Lenz , Brigitte Pautz , Winfried Albert
发明人： Joachim Siedel , Ulrich Neumann , Joachim Ziegenhorn , Hans-Georg Batz , Helmut Lenz , Brigitte Pautz , Winfried Albert
IPC分类号： G01N33/53 , A61K38/00 , C07K16/18 , G01N33/531 , G01N33/543 , G01N33/577 , G01N33/70 , A61K39/00
CPC分类号： C07K16/18 , G01N33/531 , G01N33/54306 , G01N33/577 , G01N33/70 , A61K38/00
摘要： The present invention provides a creatinine antibody and a process for the preparation thereof, wherein a conjugate of creatinine and a material suitable for antiserum formation, which are connected via an aliphatic or araliphatic carboxylic acid as bridge member, is used as immunogen for antiserum formation.The present invention is also concerned with the use of the above antibody for the immunological determination of creatinine, wherein the antibody is incubated with a creatinine-containing sample solution, reacted with a conjugate of creatinine with a hapten carrier substance, whereby one of the components, antibody and conjugate, is present in the solid phase or in dissolved form and the other component is present in dissolved form and the inhibition of the binding reaction between the antibody and the creatinine conjugate is measured.Furthermore, the present invention provides a reagent for the immunological determination of creatinine, wherein it contains the above antibody, a conjugate of creatinine with a hapten carrier substance and buffer substance.
摘要翻译：本发明提供一种肌酸酐抗体及其制备方法，其中通过脂肪族或脂环族羧酸作为桥接剂连接肌酸酐和适于抗血清形成的物质的缀合物，用作抗血清形成的免疫原。本发明还涉及上述抗体用于肌酸酐免疫学测定的用途，其中将抗体与含肌酸酐的样品溶液一起温育，与肌酸酐的缀合物与半抗原载体物质反应，由此其中一种成分，抗体和缀合物以固相或溶解形式存在，另一组分以溶解形式存在，并测量抗体与肌酐结合物之间结合反应的抑制。此外，本发明提供了用于肌酸酐免疫测定的试剂，其中含有上述抗体，肌酸酐与半抗原载体物质和缓冲物质的结合物。

8. 发明授权

US5094943A Process and reagent for the improved quantitative colorimetic determination of hydrogen peroxide 失效
标题翻译：改进过氧化氢定量色谱法的方法和试剂
公开(公告)号：US5094943A
公开(公告)日：1992-03-10
申请号：US147576
申请日：1988-01-19
申请人： Joachim Siedel , Albert Roder , Joachim Ziegenhorn
发明人： Joachim Siedel , Albert Roder , Joachim Ziegenhorn
IPC分类号： C12Q1/26 , C12Q1/28
CPC分类号： C12Q1/28 , C12Q1/26
摘要： The present invention provides a process for the improved colorimetric determination of hydrogen peroxide as formed by a hydrogen peroxide-producing oxidase, by addition of a chromogenic system and measurement of the colored material formed, wherein superoxide dismutase (E.C. 1.15.1.1) is added to the reagent solution.The present invention also provides a reagent for the improved colorimetric determination of hydrogen peroxide, comprising a hydrogen peroxide-producing oxidase, a chromogenic system, a buffer and optionally adjuvant enzymes, wherein it also contains superoxide dismutase.
摘要翻译：本发明提供了通过添加显色系统和形成的有色材料的测量来改进由产生过氧化氢的氧化酶形成的过氧化氢的比色测定方法，其中将超氧化物歧化酶（EC 1.15.1.1）加入到试剂溶液。本发明还提供了用于改进过氧化氢比色测定的试剂，其包含产生过氧化氢的氧化酶，显色系统，缓冲液和任选的辅助酶，其中还含有超氧化物歧化酶。

9. 发明授权

US4895799A Process and reagent for the determination of total bilirubin 失效
标题翻译：用于测定总胆红素的方法和试剂
公开(公告)号：US4895799A
公开(公告)日：1990-01-23
申请号：US17662
申请日：1987-02-24
申请人： Cornelia Kruse-Muller , Joachim Siedel , Joachim Ziegenhorn
发明人： Cornelia Kruse-Muller , Joachim Siedel , Joachim Ziegenhorn
IPC分类号： C12Q1/26 , C12Q1/37 , G01N33/72
CPC分类号： C12Q1/26 , C12Q1/37 , Y10S435/81
摘要： The present invention provides a process for the determination of total bilirubin in samples of body fluids. The method comprises the incubation of a sample of body fluid with both subtilisin which completely liberates bilirubin from its albumin and with bilirubin oxidase as a measurement of total bilirubin content in said body fluid sample.
摘要翻译：本发明提供了一种测定体液样品中总胆红素的方法。该方法包括将体液样品与从其白蛋白完全释放胆红素的枯草杆菌蛋白酶和胆红素氧化酶一起孵育，作为所述体液样品中总胆红素含量的量度。

10. 发明授权

US4727025A Process and reagent for the fully enzymatic determination of urea 失效
标题翻译：用于全面酶法测定尿素的方法和试剂
公开(公告)号：US4727025A
公开(公告)日：1988-02-23
申请号：US823489
申请日：1986-01-10
申请人： Joachim Siedel , August W. Wahlefeld , Joachim Ziegenhorn
发明人： Joachim Siedel , August W. Wahlefeld , Joachim Ziegenhorn
IPC分类号： G01N33/62 , C12N9/04 , C12Q1/56 , C12Q1/58
CPC分类号： C12N9/0006 , C12Q1/58 , C12Y101/01154
摘要： For the enzymatic determination of urea, one reacts the urea with glyoxylate in the presence of ureidoglycolate synthetase to give (S)-ureidoglycolate and oxidizes the latter with NAD(P).sup.+ and ureidoglycolate dehydrogenase to carbamoyloxamate and measures NAD(P)H formed either directly or via a color indicator system. A reagent suitable herefor contains glyoxylate, NAD(P).sup.+, (S)-ureidoglycolate synthetase, ureidoglycolate dehydrogenase and buffer substance.
摘要翻译： PCT No.PCT / EP85 / 00250 Sec。 371日期1986年1月10日 102（e）日期1986年1月10日PCT提交1985年5月23日PCT公布。出版物WO85 / 05641 1985年12月19日。尿素的酶法测定中，尿素与脲醛酸酯在脲基乙酸合成酶的存在下反应，得到（S） - 脲基乙醇酸酯，并用NAD（P）+和脲基乙酸脱氢酶将其氧化成氨基甲酰氨基甲酸酯 NAD（P）H直接或通过颜色指示器系统形成。适用于此的试剂含有乙醛酸，NAD（P）+，（S） - 脲基乙酸合成酶，脲基乙酸脱氢酶和缓冲物质。

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