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1. 发明申请

US20100174058A1 FLUORESCENT QUENCHING DETECTING REAGENTS AND METHODS 有权
标题翻译：荧光猝灭检测试剂和方法
公开(公告)号：US20100174058A1
公开(公告)日：2010-07-08
申请号：US12683326
申请日：2010-01-06
申请人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M.J. Vermeulen
发明人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M.J. Vermeulen
IPC分类号： C07H19/16 , C07H21/00
CPC分类号： C07H21/00 , C07B2200/11 , C07C245/08 , C07D519/00 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/04 , C09B29/0813 , C09B69/106 , C12Q1/6818 , C40B40/00 , C12Q2565/1025 , C12Q2521/319
摘要： Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
摘要翻译：提供了含有两个标记的寡核苷酸探针，可用于杂交测定。探针也可以包含一个小沟结合组。

2. 发明授权

US06727356B1 Fluorescent quenching detection reagents and methods 有权
标题翻译：荧光猝灭检测试剂及方法
公开(公告)号：US06727356B1
公开(公告)日：2004-04-27
申请号：US09457616
申请日：1999-12-08
申请人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy
发明人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy
IPC分类号： C07H1900
CPC分类号： C07D519/00 , C07B2200/11 , C07C245/08 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/00 , C09B29/0813 , C12Q1/6818 , C40B40/00 , C12Q2565/1025 , C12Q2521/319
摘要： Oligonucleotide-fluorophore-quencher conjugates wherein the fluorophore moiety has emission wavelengths in the range of about 300 to about 800 nm, and or where the quencher includes a substituted 4-(phenyldiazenyl)phenylamine structure provide improved signal to noise ratios and other advantageous characteristics in hybridization and related assays. The oligonucleotide-fluorophore-quencher conjugates can be synthesized by utilizing novel phosphoramidite reagents that incorporate the quencher moiety based on the substituted 4-(phenyldiazenyl)phenylamine structure, and or novel phosphoramidite reagents that incorporate a fluorophore moiety based on the substituted coumarin, substituted 7-hydroxy-3H-phenoxazin-3-one, or substituted 5,10-dihydro-10-[phenyl]pyrido[2,3-d;6,5-d′]dipyrimidine-2,4,6,8-(1H,3H,7H,9H,10H)-tetrone structure. Oligonucleotide-fluorophore-quencher-minor groove binder conjugates including a pyrrolo[4,5-e]indolin-7-yl}carbonyl)pyrrolo[4,5-e]indolin-7-yl]carbonyl}pyrrolo[4,5-e]indoline-7-carboxylate (DPI3) moiety as the minor groove binder and the substituted 4-(phenyldiazenyl)phenylamine moiety as the quencher, were synthesized and have substantially improved hybridization and signal to noise ratio properties.
摘要翻译：寡核苷酸 - 荧光团 - 猝灭剂缀合物，其中荧光团部分具有在约300至约800nm范围内的发射波长，或者猝灭剂包含取代的4-（苯基二氮烯基）苯胺结构的提供改善的信噪比和其它有利特征杂交和相关测定。寡核苷酸 - 荧光团 - 猝灭剂缀合物可以通过利用引入基于取代的4-（苯基二氮烯基）苯基胺结构的猝灭剂部分的新型亚磷酰胺试剂，和/或新型亚磷酰胺试剂合成，所述亚磷酰胺试剂掺入基于取代香豆素的荧光团部分，取代7 - 羟基-3H-吩恶嗪-3-酮或取代的5,10-二氢-10- [苯基]吡啶并[2,3-d; 6,5-d']二嘧啶-2,4,6,8-（ 1H，3H，7H，9H，10H） - 四酮结构。吡咯并[4,5-e]二氢吲哚-7-基}羰基）吡咯并[4,5-e]二氢吲哚-7-基]羰基}吡咯并[4,5- e]二氢吲哚-7-羧酸酯（DPI 3）部分作为次槽粘合剂和取代的4-（苯基二氮烯基）苯胺部分作为猝灭剂，并且具有显着改善的杂交和信噪比性质。

3. 发明授权

US07897736B2 Fluorescent quenching detecting reagents and methods 有权
标题翻译：荧光猝灭检测试剂及方法
公开(公告)号：US07897736B2
公开(公告)日：2011-03-01
申请号：US12683326
申请日：2010-01-06
申请人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M. J. Vermeulen
发明人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M. J. Vermeulen
IPC分类号： C07G3/00 , C07H21/00 , C07H21/02 , C07H19/04 , C12Q1/68
CPC分类号： C07H21/00 , C07B2200/11 , C07C245/08 , C07D519/00 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/04 , C09B29/0813 , C09B69/106 , C12Q1/6818 , C40B40/00 , C12Q2565/1025 , C12Q2521/319
摘要： Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
摘要翻译：提供了含有两个标记的寡核苷酸探针，可用于杂交测定。探针也可以包含一个小沟结合组。

4. 发明授权

US06653473B2 Fluorescent quenching detection reagents and methods 有权
公开(公告)号：US06653473B2
公开(公告)日：2003-11-25
申请号：US10084818
申请日：2002-02-26
申请人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M. J. Vermeulen
发明人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M. J. Vermeulen
IPC分类号： C07D28816
CPC分类号： C07D519/00 , C07B2200/11 , C07C245/08 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/00 , C09B29/0813 , C12Q1/6818 , C40B40/00 , C12Q2565/1025 , C12Q2521/319
摘要： Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.

5. 发明授权

US07662942B2 Fluorescent quenching detection reagents and methods 有权
标题翻译：荧光猝灭检测试剂及方法
公开(公告)号：US07662942B2
公开(公告)日：2010-02-16
申请号：US11338866
申请日：2006-01-23
申请人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M. J. Vermeulen
发明人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M. J. Vermeulen
IPC分类号： C07H21/00 , C07H21/02 , C12Q1/68
CPC分类号： C07H21/00 , C07B2200/11 , C07C245/08 , C07D519/00 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/04 , C09B29/0813 , C09B69/106 , C12Q1/6818 , C40B40/00 , C12Q2565/1025 , C12Q2521/319
摘要： Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
摘要翻译：提供了含有两个标记的寡核苷酸探针，可用于杂交测定。探针也可以包含一个小沟结合组。

6. 发明授权

US06699975B2 Fluorescent quenching detection reagents and methods 有权
标题翻译：荧光猝灭检测试剂及方法
公开(公告)号：US06699975B2
公开(公告)日：2004-03-02
申请号：US10093769
申请日：2002-03-07
申请人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy
发明人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy
IPC分类号： C07C24500
CPC分类号： C07D519/00 , C07B2200/11 , C07C245/08 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/00 , C09B29/0813 , C12Q1/6818 , C40B40/00 , C12Q2565/1025 , C12Q2521/319
摘要： Oligonucleotide-fluorophore-quencher conjugates wherein the fluorophore moiety has emission wavelengths in the range of about 300 to about 800 nm, and or where the quencher includes a substituted 4-(phenyldiazenyl)phenylamine structure provide improved signal to noise ratios and other advantageous characteristics in hybridization and related assays. The oligonucleotide-fluorophore-quencher conjugates can be synthesized by utilizing novel phosphoramidite reagents that incorporate the quencher moiety based on the substituted 4-(phenyidiazenyl)phenylamine structure, and or novel phosphoramidite reagents that incorporate a fluorophore moiety based on the substituted coumarin, substituted 7-hydroxy-3H-phenoxazin-3-one, or substituted 5,10-dihydro-10-[phenyl]pyrido[2,3-d;6,5-d′]dipyrimidine-2,4,6,8-(1H,3H,7H,9H,10H)-tetrone structure. Oligonucleotide-fluorophore-quencher-minor groove binder conjugates including a pyrrolo[4,5-e]indolin-7-yl}carbonyl) pyrrolo[4,5-e]indolin-7-yl]carbonyl}pyrrolo[4,5-e]indoline-7-carboxylate (DPI3) moiety as the minor groove binder and the substituted 4-(phenyldiazenyl)phenylamine moiety as the quencher, were synthesized and have substantially improved hybridization and signal to noise ratio properties.
摘要翻译：寡核苷酸 - 荧光团 - 猝灭剂缀合物，其中荧光团部分具有在约300至约800nm范围内的发射波长，或者其中猝灭剂包括取代的4-（苯基二氮烯基）苯胺结构提供改善的信噪比和其它有利特征杂交和相关测定。寡核苷酸 - 荧光团 - 猝灭剂缀合物可以通过利用基于取代的4-（苯基亚基）苯胺结构的掺入猝灭剂部分的新型亚磷酰胺试剂和/或掺入基于取代香豆素的荧光团部分的新型亚磷酰胺试剂来合成，取代7 - 羟基-3H-吩恶嗪-3-酮或取代的5,10-二氢-10- [苯基]吡啶并[2,3-d; 6,5-d']二嘧啶-2,4,6,8-（ 1H，3H，7H，9H，10H） - 四酮结构。吡咯并[4,5-e]二氢吲哚-7-基}羰基）吡咯并[4,5-e]二氢吲哚-7-基]羰基}吡咯并[4,5- e]二氢吲哚-7-羧酸酯（DPI 3）部分作为次槽粘合剂和取代的4-（苯基二氮烯基）苯胺部分作为猝灭剂，并且具有显着改善的杂交和信噪比性质。

7. 发明授权

US06683173B2 Tm leveling methods 有权
标题翻译：调平方法
公开(公告)号：US06683173B2
公开(公告)日：2004-01-27
申请号：US10032307
申请日：2001-12-21
申请人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
发明人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
IPC分类号： C07H2104
CPC分类号： C07H21/00 , C07B2200/11 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H19/23 , C12Q1/6832 , C40B40/00 , G06F19/22 , C12Q2525/117
摘要： Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
摘要翻译：提供了修饰的寡核苷酸，其含有选自未取代的和3-取代的吡唑并[3,4-d]嘧啶和5-取代的嘧啶的碱，并且任选具有连接的小沟结合剂和报道基团。

8. 发明授权

US06660845B1 Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof 有权
标题翻译：包含核苷酸类似物的非聚集性非猝灭性低聚物; 合成和使用方法
公开(公告)号：US06660845B1
公开(公告)日：2003-12-09
申请号：US09447936
申请日：1999-11-23
申请人： Alexander A. Gall , Igor V. Kutyavin , Nicolaas M. J. Vermeulen , Robert O. Dempcy
发明人： Alexander A. Gall , Igor V. Kutyavin , Nicolaas M. J. Vermeulen , Robert O. Dempcy
IPC分类号： C07H2100
CPC分类号： C07D487/04 , A61K31/519 , C07H21/00 , C07K5/06026 , C07K5/06139 , C07K14/003 , C12Q1/6818 , C12Q1/6832 , C12Q2525/107 , C12Q2525/101
摘要： The invention provides compositions and methods for improved hybridization analysis utilizing DNA, RNA, PNA and chimeric oligomers in which one or more purine bases are substituted by a pyrazolo[5,4-d]pyrimidine or by a 7-deazapurine purine analogue. Reduced self-aggregation and reduced fluorescence quenching are obtained when the oligomers are used in various methods involving hybridization. Methods of synthesis, as well as novel synthetic precursors, are also provided.
摘要翻译：本发明提供使用DNA，RNA，PNA和嵌合寡聚物改进杂交分析的组合物和方法，其中一个或多个嘌呤碱基被吡唑并[5,4-d]嘧啶或7-脱氮嘌呤嘌呤类似物取代。当寡聚物以涉及杂交的各种方法使用时，获得了减少的自聚集和减少的荧光猝灭。还提供了合成方法，以及新型合成前体。

9. 发明授权

US06972328B2 Non-aggregating non-quenching oligomer comprising nucelotide analogs, method of synthesis and use thereof 有权
标题翻译：包含芥菜素类似物的非聚集性非猝灭寡聚物，其合成方法和用途
公开(公告)号：US06972328B2
公开(公告)日：2005-12-06
申请号：US10702007
申请日：2003-11-04
申请人： Alexander A. Gall , Igor V. Kutyavin , Nicolaas M. J. Vermeulen , Robert O. Dempcy
发明人： Alexander A. Gall , Igor V. Kutyavin , Nicolaas M. J. Vermeulen , Robert O. Dempcy
IPC分类号： G01N33/53 , A61K31/519 , A61K48/00 , C07D487/04 , C07H21/00 , C07H21/04 , C07K5/06 , C07K5/078 , C07K14/00 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N33/50 , G01N33/58 , C07H21/02
CPC分类号： C07D487/04 , A61K31/519 , C07H21/00 , C07K5/06026 , C07K5/06139 , C07K14/003 , C12Q1/6818 , C12Q1/6832 , C12Q2525/107 , C12Q2525/101
摘要： The invention provides compositions and methods for improved hybridization analysis utilizing DNA, RNA, PNA and chimeric oligomers in which one or more purine bases are substituted by a pyrazolo[5,4-d]pyrimidine or by a 7-deazapurine purine analogue. Reduced self-aggregation and reduced fluorescence quenching are obtained when the oligomers are used in various methods involving hybridization. Methods of synthesis, as well as novel synthetic precursors, are also provided.
摘要翻译：本发明提供使用DNA，RNA，PNA和嵌合寡聚物改进杂交分析的组合物和方法，其中一个或多个嘌呤碱基被吡唑并[5,4-d]嘧啶或7-脱氮嘌呤嘌呤类似物取代。当寡聚物以涉及杂交的各种方法使用时，获得了减少的自聚集和减少的荧光猝灭。还提供了合成方法，以及新型合成前体。

10. 发明授权

US07715989B2 Systems and methods for predicting oligonucleotide melting temperature (TmS) 有权
标题翻译：用于预测寡核苷酸融合温度（TmS）的系统和方法
公开(公告)号：US07715989B2
公开(公告)日：2010-05-11
申请号：US10176972
申请日：2002-06-18
申请人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Karen M. Singer, legal representative , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
发明人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
IPC分类号： G01N33/48
CPC分类号： G06F19/20 , C07B2200/11 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H19/23 , C07H21/00 , C12Q1/6813 , C12Q1/6827 , C12Q1/6858 , C12Q2600/156 , C40B40/00 , G06F19/22 , C12Q2563/173 , C12Q2527/107 , C12Q2525/117 , C12Q2525/197 , C12Q2541/101 , C12Q2535/125
摘要： Software systems and methods for predicting the melting temperature (Tm) and other characteristics of oligonucleotides, including modified oligonucleotides. Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
摘要翻译：用于预测寡核苷酸的融解温度（Tm）和其他特征的软件系统和方法，包括修饰的寡核苷酸。提供了修饰的寡核苷酸，其含有选自未取代的和3-取代的吡唑并[3,4-d]嘧啶和5-取代的嘧啶的碱，并且任选具有连接的小沟结合剂和报道基团。

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