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1. 发明授权

US5614362A Nucleic acid hybridization assay for hepatitis B virus DNA 失效
标题翻译：乙型肝炎病毒DNA的核酸杂交检测
公开(公告)号：US5614362A
公开(公告)日：1997-03-25
申请号：US315685
申请日：1994-09-30
申请人： Michael S. Urdea , Brian Warner , Joyce A. Running , Janice A. Kolberg , Jennifer M. Clyne , Ray Sanchez-Pescador , Thomas Horn
发明人： Michael S. Urdea , Brian Warner , Joyce A. Running , Janice A. Kolberg , Jennifer M. Clyne , Ray Sanchez-Pescador , Thomas Horn
IPC分类号： C07H19/06 , C07H21/00 , C12Q1/68 , C12Q1/70 , G01N33/532
CPC分类号： C07H19/06 , C07H21/00 , C12Q1/682 , C12Q1/689 , C12Q1/706 , G01N33/532 , G01N2333/045 , Y10S435/81 , Y10S435/948 , Y10S436/811
摘要： Linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit that is complementary to a single-stranded oligonucleotide sequence of interest, and (2) a multiplicity of second single-stranded oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are exemplified.
摘要翻译：用作生物化学测定中放大器的线性或支化寡核苷酸多聚体，其包含（1）与目的单链寡核苷酸序列互补的至少一个第一单链寡核苷酸单元，和（2）多个第二单链寡核苷酸与单链标记的寡核苷酸互补的单元。示例了使用多聚体的扩增的夹心核酸杂交和免疫测定。

2. 发明授权

US5849481A Nucleic acid hybridization assays employing large comb-type branched polynucleotides 失效
标题翻译：使用大梳状支链多核苷酸的核酸杂交测定
公开(公告)号：US5849481A
公开(公告)日：1998-12-15
申请号：US470124
申请日：1995-06-05
申请人： Michael S. Urdea , Thomas Horn , Chu-An Chang , Brian Warner , Timothy J. Fultz
发明人： Michael S. Urdea , Thomas Horn , Chu-An Chang , Brian Warner , Timothy J. Fultz
IPC分类号： C07H21/00 , C12Q1/68
CPC分类号： C07H21/00 , C12Q1/682
摘要： Comb-type branched polynucleotides are used as amplification multimers in nucleic acid hybridization assays.
摘要翻译：梳状支化多核苷酸用作核酸杂交测定中的扩增多聚体。

3. 发明授权

US5124246A Nucleic acid multimers and amplified nucleic acid hybridization assays using same 失效
标题翻译：使用相同的核酸多聚体和放大的核酸混合测定
公开(公告)号：US5124246A
公开(公告)日：1992-06-23
申请号：US340031
申请日：1989-04-18
申请人： Michael S. Urdea , Brian Warner , Thomas Horn
发明人： Michael S. Urdea , Brian Warner , Thomas Horn
IPC分类号： C07H19/06 , C07H21/00 , C12Q1/68 , C12Q1/70 , G01N33/532
CPC分类号： C07H19/06 , C07H21/00 , C12Q1/682 , C12Q1/689 , C12Q1/706 , G01N33/532 , G01N2333/045 , Y10S435/81
摘要： Linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit that is complementary to a single-stranded oligonucleotide sequence of interest, and (2) a multiplicity of second single-stranded oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are exemplified.
摘要翻译：用作生物化学测定中放大器的线性或支化寡核苷酸多聚体，其包含（1）与目的单链寡核苷酸序列互补的至少一个第一单链寡核苷酸单元，和（2）多个第二单链寡核苷酸与单链标记的寡核苷酸互补的单元。示例了使用多聚体的扩增的夹心核酸杂交和免疫测定。

4. 发明授权

US5624802A Nucleic acid multimers and amplified nucleic acid hybridization assays using same 失效
标题翻译：使用相同的核酸多聚体和扩增的核酸杂交测定法
公开(公告)号：US5624802A
公开(公告)日：1997-04-29
申请号：US479493
申请日：1995-06-07
申请人： Michael S. Urdea , Brian Warner , Thomas Horn
发明人： Michael S. Urdea , Brian Warner , Thomas Horn
IPC分类号： C07H19/06 , C07H21/00 , C12Q1/68 , C12Q1/70 , G01N33/532 , C07H21/04
CPC分类号： C07H19/06 , C07H21/00 , C12Q1/682 , C12Q1/689 , C12Q1/706 , G01N33/532 , G01N2333/045 , Y10S435/81 , Y10S435/948 , Y10S436/811
摘要： Linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit that is complementary to a single-stranded oligonucleotide sequence of interest, and (2) a multiplicity of second single-stranded oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are exemplified.
摘要翻译：用作生物化学测定中放大器的线性或支化寡核苷酸多聚体，其包含（1）与目的单链寡核苷酸序列互补的至少一个第一单链寡核苷酸单元，和（2）多个第二单链寡核苷酸与单链标记的寡核苷酸互补的单元。示例了使用多聚体的扩增的夹心核酸杂交和免疫测定。

5. 发明授权

US5359100A Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers 失效
标题翻译：双功能封闭的亚磷酰胺可用于制备核酸诱变剂
公开(公告)号：US5359100A
公开(公告)日：1994-10-25
申请号：US107358
申请日：1993-08-13
申请人： Michael S. Urdea , Brian Warner , Joyce A. Running , Janice A. Kolberg , Jennifer M. Clyne , Ray Sanchez-Pescador , Thomas Horn
发明人： Michael S. Urdea , Brian Warner , Joyce A. Running , Janice A. Kolberg , Jennifer M. Clyne , Ray Sanchez-Pescador , Thomas Horn
IPC分类号： C07H19/06 , C07H21/00 , C12Q1/68 , C12Q1/70 , G01N33/532 , C07F9/24
CPC分类号： C07H19/06 , C07H21/00 , C12Q1/682 , C12Q1/689 , C12Q1/706 , G01N33/532 , G01N2333/045 , Y10S435/81 , Y10S435/948 , Y10S436/811
摘要： Linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit that is complementary to a single-stranded oligonucleotide sequence of interest, and (2) a multiplicity of second single-stranded oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are exemplified.
摘要翻译：用作生物化学测定中放大器的线性或支化寡核苷酸多聚体，其包含（1）与目的单链寡核苷酸序列互补的至少一个第一单链寡核苷酸单元，和（2）多个第二单链寡核苷酸与单链标记的寡核苷酸互补的单元。示例了使用多聚体的扩增的夹心核酸杂交和免疫测定。

6. 发明授权

US5710264A Large comb type branched polynucleotides 失效
标题翻译：大梳型支链多核苷酸
公开(公告)号：US5710264A
公开(公告)日：1998-01-20
申请号：US478085
申请日：1995-06-07
申请人： Michael S. Urdea , Thomas Horn , Chu-An Chang , Brian Warner , Timothy J. Fultz
发明人： Michael S. Urdea , Thomas Horn , Chu-An Chang , Brian Warner , Timothy J. Fultz
IPC分类号： C07H21/00 , C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C07H21/00 , C12Q1/682
摘要： Large comb-type branched polynucleotides useful as amplifier molecules in nucleic acid hybridization assays are provided, the polynucleotides comprising: a polynucleotide backbone having at least about 15 multifunctional nucleotides, each of which defines a sidechain site and a first single-stranded oligonucleotide unit that is capable of binding specifically to a first single-stranded polynucleotide sequence of interest; and pendant polynucleotide sidechains extending from said multifunctional nucleotides each comprising iterations of a second single-stranded oligonucleotide unit that is capable of binding specifically to a second single-stranded polynucleotide sequence of interest, the total number of iterations in all sidechains being at least 20. Methods of making these polynucleotides are also provided.
摘要翻译：提供了用作核酸杂交测定中的放大分子的大梳状支化多核苷酸，所述多核苷酸包含：多核苷酸主链，其具有至少约15个多功能核苷酸，每个核苷酸限定侧链位点和第一单链寡核苷酸单元，能够特异性结合目标的第一单链多核苷酸序列; 和从所述多功能核苷酸延伸的侧挂多核苷酸侧链，其每个包括能够特异性结合目标的第二单链多核苷酸序列的第二单链寡核苷酸单元的迭代，所有侧链的总迭代次数至少为20。还提供制备这些多核苷酸的方法。

7. 发明申请

US20070231821A1 Methods and compositions for detecting polynucleotides 有权
标题翻译：用于检测多核苷酸的方法和组合物
公开(公告)号：US20070231821A1
公开(公告)日：2007-10-04
申请号：US11710667
申请日：2007-02-23
申请人： Charles Bupp , K. Choi , Rachel Holmes-Davis , Alexander Izmailov , Heather Koshinsky , Christopher Nulf , Mickey Urdea , Miaomiao Wang , Brian Warner , Michael Zwick
发明人： Charles Bupp , K. Choi , Rachel Holmes-Davis , Alexander Izmailov , Heather Koshinsky , Christopher Nulf , Mickey Urdea , Miaomiao Wang , Brian Warner , Michael Zwick
IPC分类号： C12Q1/68 , C07D417/02 , C07D413/02 , C07D401/02
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q2600/156
摘要： Methods of determining the presence or amount of a target polynucleotide in a sample are provided. A sample that contains a target polynucleotide, a polynucleotide that may include nucleic acid analogs and that is complementary to a target nucleic acid sequence of the target polynucleotide, and a dye for which the rate of change in an optical property is different in the presence and absence of a target polynucleotide/polynucleotide hybrid are combined to produce a reaction mixture. The rate of change in an optical property of the dye in the reaction mixture is compared to a reference value characteristic of the rate of change in the optical property of the dye in a similar reaction mixture containing a known amount of a polynucleotide/polynucleotide hybrid to determine a relative rate of change in the optical property. The relative rate of change in the optical property of dye in the reaction mixture is correlated with the presence or amount of the specified target polynucleotide in the sample.
摘要翻译：提供了确定样品中靶多核苷酸的存在或量的方法。含有靶多核苷酸，可包括核酸类似物并与靶多核苷酸的靶核酸序列互补的多核苷酸的样品和在存在和/或存在的情况下光学性质的变化率不同的染料组合不存在靶多核苷酸/多核苷酸杂交体以产生反应混合物。将反应混合物中染料的光学性质的变化率与含有已知量的多核苷酸/多核苷酸杂化物的类似反应混合物中的染料的光学性质变化率的变化率的参考值进行比较确定光学性质的相对变化率。反应混合物中染料的光学性质的相对变化率与样品中特定靶多核苷酸的存在或数量相关。

8. 发明申请

US20060172284A1 Highly orthogonal universal sequences for use in nucleic acid assays 有权
标题翻译：用于核酸测定的高度正交通用序列
公开(公告)号：US20060172284A1
公开(公告)日：2006-08-03
申请号：US11049465
申请日：2005-02-01
申请人： Minxue Zheng , David Ahle , Brian Warner , Ming Wu , Chu-an Chang
发明人： Minxue Zheng , David Ahle , Brian Warner , Ming Wu , Chu-an Chang
IPC分类号： C12Q1/70 , C12Q1/68 , C07H21/02 , C12P19/34
CPC分类号： C12Q1/682 , Y02A50/54 , C12Q2525/101
摘要： The invention provides a set of highly orthogonal six-code universal sequences for use in bDNA singleplex and multiplex nucleic acid hybridization assays. The six-code orthogonal sequences do not cross-hybridize and thus, minimize or eliminate the 3-mer cross-hybridization inherent in the second and third generation bDNA assays. The highly orthogonal universal sequences may be used in singleplex or multiplex bDNA assays quantitatively and qualitatively to determine mRNA levels in a sample; to screen for and genotype targets, such as viruses, that are present in low volumes in a sample; to screen for and genotype SNPs; and to measure changes in the amount of a gene in a sample such as when gene amplifications or deletions occur. The highly orthogonal universal sequences may also be used as universal capture probes to selectively bind assay components in a way that facilitates their further analysis.
摘要翻译：本发明提供了一组高度正交的六码通用序列，用于bDNA单链和多重核酸杂交测定。六码正交序列不交叉杂交，因此最小化或消除第二代和第三代bDNA测定中固有的3聚体交叉杂交。高度正交的通用序列可以定量和定性地用于单链或多重bDNA测定以确定样品中的mRNA水平; 筛选和基因型样品中低体积存在的目标，如病毒; 筛选和基因型SNP; 并且测量样品中基因的量的变化，例如当发生基因扩增或缺失时。高度正交的通用序列还可以用作通用捕获探针，以便于进一步分析的方式选择性地结合测定组分。

9. 发明授权

US08063196B2 Highly orthogonal universal sequences for use in nucleic acid assays 有权
标题翻译：用于核酸测定的高度正交通用序列
公开(公告)号：US08063196B2
公开(公告)日：2011-11-22
申请号：US11049465
申请日：2005-02-01
申请人： Minxue Zheng , David Ahle , Brian Warner , Ming Wu , Chu-an Chang
发明人： Minxue Zheng , David Ahle , Brian Warner , Ming Wu , Chu-an Chang
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/682 , Y02A50/54 , C12Q2525/101
摘要： The invention provides a set of highly orthogonal six-code universal sequences for use in bDNA singleplex and multiplex nucleic acid hybridization assays. The six-code orthogonal sequences do not cross-hybridize and thus, minimize or eliminate the 3-mer cross-hybridization inherent in the second and third generation bDNA assays. The highly orthogonal universal sequences may be used in singleplex or multiplex bDNA assays quantitatively and qualitatively to determine mRNA levels in a sample; to screen for and genotype targets, such as viruses, that are present in low volumes in a sample; to screen for and genotype SNPs; and to measure changes in the amount of a gene in a sample such as when gene amplifications or deletions occur. The highly orthogonal universal sequences may also be used as universal capture probes to selectively bind assay components in a way that facilitates their further analysis.
摘要翻译：本发明提供了一组高度正交的六码通用序列，用于bDNA单链和多重核酸杂交测定。六码正交序列不交叉杂交，因此最小化或消除第二代和第三代bDNA测定中固有的3聚体交叉杂交。高度正交的通用序列可以定量和定性地用于单链或多重bDNA测定以确定样品中的mRNA水平; 筛选和基因型样品中低体积存在的目标，如病毒; 筛选和基因型SNP; 并且测量样品中基因的量的变化，例如当发生基因扩增或缺失时。高度正交的通用序列还可以用作通用捕获探针，以便于进一步分析的方式选择性地结合测定组分。

10. 发明授权

US07708242B1 Bucket holding apparatus and associated method 失效
标题翻译：铲斗装置及相关方法
公开(公告)号：US07708242B1
公开(公告)日：2010-05-04
申请号：US11900093
申请日：2007-09-10
申请人： Chris Petrashune , Michael Cordeaux , Brian Warner
发明人： Chris Petrashune , Michael Cordeaux , Brian Warner
IPC分类号： A47G23/02
CPC分类号： B44D3/14 , B01F15/00733
摘要： A bucket holding apparatus includes first and second carriages with pivotally attached inner edges arranged in such a manner that the first and second carriages freely rotate upwardly from a horizontal plane defined on a support surface. The bucket holding apparatus further includes first and second arcuate walls directly conjoined to the first and second carriages and oppositely situated from the inner edges such that the first and second arcuate walls define a circular cavity within a combined perimeter thereof. The bucket holding apparatus further includes an arcuate lever directly and statically conjoined to the second carriage and extending laterally upwards away from an outer edge thereof.
摘要翻译：铲斗保持装置包括具有可枢转地附接的内边缘的第一和第二托架，其以这样的方式布置，使得第一和第二托架从限定在支撑表面上的水平面自由地向上旋转。铲斗保持装置还包括直接结合到第一和第二托架并且与内边缘相对定位的第一和第二弓形壁，使得第一和第二弓形壁在其组合的周边内限定圆形空腔。铲斗保持装置还包括直接并静态地结合到第二托架并且横向向外远离其外边缘延伸的弓形杆。

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