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1. 发明授权

US08911946B2 Detection of antibiotic-resistant microorganisms 有权
标题翻译：检测抗生素抗性微生物
公开(公告)号：US08911946B2
公开(公告)日：2014-12-16
申请号：US12340622
申请日：2008-12-19
申请人： Michael M. Becker , Kui Gao , Wai-Chung Lam
发明人： Michael M. Becker , Kui Gao , Wai-Chung Lam
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/689 , C12Q2600/16 , C12Q2545/114 , C12Q2545/101 , C12Q2531/113
摘要： Method of detecting methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) in a nucleic acid coamplification assay. The invention advantageously reduces the incidence of false-positive MRSA determinations in real-time assays by requiring satisfaction of a threshold criterion that excludes certain co-infections from the MRSA determination. The invention further provides for determination of MSSA, even when the MSSA is present in combination with methicillin-resistant coagulase-negative (MR-CoNS) bacteria at high or low levels.
摘要翻译：在核酸共扩增测定中检测耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感性金黄色葡萄球菌（MSSA）的方法。本发明有利地通过要求满足从MRSA测定中排除某些共感染的阈值标准来降低实时分析中假阳性MRSA测定的发生率。本发明还提供MSSA的测定，即使MSSA与高水平或低水平的耐甲氧西林凝固酶阴性（MR-CoNS）细菌组合存在。

2. 发明授权

US08420317B2 Alkaline shock-based method of processing a biological sample 有权
标题翻译：碱性冲击法处理生物样品
公开(公告)号：US08420317B2
公开(公告)日：2013-04-16
申请号：US13307991
申请日：2011-11-30
申请人： Kui Gao , Michael M. Becker , Wen Wu , Jeffrey M. Linnen
发明人： Kui Gao , Michael M. Becker , Wen Wu , Jeffrey M. Linnen
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N15/1006 , C12N15/1013 , C12Q1/6806 , C12Q1/689 , C12Q2527/119
摘要： Method of processing a biological sample to yield nucleic acid appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.
摘要翻译：处理生物样品以产生适合用于随后的体外核酸扩增反应的核酸的方法。该方法包括快速，瞬时暴露于碱性条件，这可以通过将碱性溶液与包含洗涤剂的pH缓冲溶液和待测试的生物样品混合使用体外扩增来存在特定的核酸物种来实现。本发明的方法有利地可以改善某些靶核酸的检测，而不会显着损害其他目标核酸的可检测性。该方法特别适用于同时制备可用于多重扩增反应的RNA和DNA模板。

3. 发明授权

US08071301B2 Method of isolating nucleic acids from a biological sample 有权
标题翻译：从生物样品中分离核酸的方法
公开(公告)号：US08071301B2
公开(公告)日：2011-12-06
申请号：US12827605
申请日：2010-06-30
申请人： Kui Gao , Michael M. Becker , Wen Wu , Jeffrey M. Linnen
发明人： Kui Gao , Michael M. Becker , Wen Wu , Jeffrey M. Linnen
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1013 , C12Q1/6806 , C12Q1/689 , C12Q2527/119
摘要： Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.
摘要翻译：制备适合用于随后体外核酸扩增反应的生物样品的方法。该方法包括快速，瞬时暴露于碱性条件，这可以通过将碱性溶液与包含洗涤剂的pH缓冲溶液和待测试的生物样品混合使用体外扩增来存在特定的核酸物种来实现。本发明的方法有利地可以改善某些靶核酸的检测，而不会显着损害其他目标核酸的可检测性。该方法特别适用于同时制备可用于多重扩增反应的RNA和DNA模板。

4. 发明授权

US07803581B2 Alkaline shock-based preparation of nucleic acids 有权
标题翻译：基于碱性休克的核酸制备
公开(公告)号：US07803581B2
公开(公告)日：2010-09-28
申请号：US12409818
申请日：2009-03-24
申请人： Kui Gao , Michael M. Becker , Wen Wu , Jeffrey M. Linnen
发明人： Kui Gao , Michael M. Becker , Wen Wu , Jeffrey M. Linnen
IPC分类号： C12P19/34 , C07H1/08
CPC分类号： C12N15/1006 , C12N15/1013 , C12Q1/6806 , C12Q1/689 , C12Q2527/119
摘要： Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.
摘要翻译：制备适合用于随后体外核酸扩增反应的生物样品的方法。该方法包括快速，瞬时暴露于碱性条件，这可以通过将碱性溶液与包含洗涤剂的pH缓冲溶液和待测试的生物样品混合使用体外扩增来存在特定的核酸物种来实现。本发明的方法有利地可以改善某些靶核酸的检测，而不会显着损害其他目标核酸的可检测性。该方法特别适用于同时制备可用于多重扩增反应的RNA和DNA模板。

5. 发明授权

US07510837B2 Sample preparation method incorporating an alkaline shock 有权
标题翻译：掺入碱性休克的样品制备方法
公开(公告)号：US07510837B2
公开(公告)日：2009-03-31
申请号：US11356613
申请日：2006-02-17
申请人： Kui Gao , Michael M. Becker , Wen Wu , Jeffrey M. Linnen
发明人： Kui Gao , Michael M. Becker , Wen Wu , Jeffrey M. Linnen
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1013 , C12Q1/6806 , C12Q1/689 , C12Q2527/119
摘要： Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.
摘要翻译：制备适合用于随后体外核酸扩增反应的生物样品的方法。该方法包括快速，瞬时暴露于碱性条件，这可以通过将碱性溶液与包含洗涤剂的pH缓冲溶液和待测试的生物样品混合使用体外扩增来存在特定的核酸物种来实现。本发明的方法有利地可以改善某些靶核酸的检测，而不会显着损害其他目标核酸的可检测性。该方法特别适用于同时制备可用于多重扩增反应的RNA和DNA模板。

6. 发明授权

US09752201B2 Compositions and method for detecting human parvovirus nucleic acid and for detecting hepatitis A virus nucleic acids in single-plex or multiplex assays 有权
公开(公告)号：US09752201B2
公开(公告)日：2017-09-05
申请号：US14232874
申请日：2012-07-13
申请人： Kui Gao , Jeffrey M. Linnen , Kurt Craft Norton , Patricia C. Gordon , Dat Do , Tan N. Le
发明人： Kui Gao , Jeffrey M. Linnen , Kurt Craft Norton , Patricia C. Gordon , Dat Do , Tan N. Le
IPC分类号： C12Q1/68 , C12Q1/70 , C07H21/02
CPC分类号： C12Q1/706 , C12Q1/701 , C12Q2600/16 , Y02A50/54
摘要： Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed.

7. 发明公开

US20230393040A1 Method and System of Sample Pooling and Processing 审中-公开
公开(公告)号：US20230393040A1
公开(公告)日：2023-12-07
申请号：US18033254
申请日：2021-10-21
申请人： Kui Gao
发明人： Kui Gao
IPC分类号： G01N1/38
CPC分类号： G01N1/38
摘要： The present disclosure includes, but is not limited to, methods and systems for pooling or combining multiple samples together. Particularly, this disclosure relates to pooling and combining samples in a process and system that combines samples in a way that allows targets in the pooled sample to be analyzed/detected with a similar sensitivity and specificity as for analysis/detection of targets from the individual samples of the pool. Specifically, in some embodiments, methods herein avoid a dilution effect found in commonly used sample pooling methods that can reduce their sensitivity and limit the number of samples that can be pooled. In some embodiments, methods herein include processes to de-convolute the pool down to individual samples. Methods herein may be applicable to creating and testing pooled/combined samples for target molecules and de-convoluting pooled samples to identify an individual sample in a pool containing the target. Systems for performing the methods, including in some embodiments partially or fully automated methods, are also described.

8. 发明授权

US08195775B2 Enhanced method and apparatus for reducing congestion in DHCP network system 有权
标题翻译：用于减少DHCP网络系统拥塞的增强方法和装置
公开(公告)号：US08195775B2
公开(公告)日：2012-06-05
申请号：US12450684
申请日：2007-04-06
申请人： Zhi Gang Zhang , Kui Gao , Jian Song Li
发明人： Zhi Gang Zhang , Kui Gao , Jian Song Li
IPC分类号： G06F15/16
CPC分类号： H04L47/10 , H04L29/12283 , H04L47/125 , H04L61/2015 , H04L61/2061
摘要： An enhanced method and apparatus for reducing congestion in dynamic host configuration protocol network system are provided. The congestion reduction method comprises steps of receiving a DHCP request from a DHCP client; and stopping responding to a certain number of DHCPDISCOVER message. And the dynamic host configuration protocol server comprises at least one distributed server, at which the contents of the network service provider server are backed up.
摘要翻译：提供了一种用于减少动态主机配置协议网络系统拥塞的增强方法和装置。所述拥塞减少方法包括从DHCP客户端接收DHCP请求的步骤; 并停止响应一定数量的DHCPDISCOVER消息。并且动态主机配置协议服务器包括至少一个分布式服务器，在该服务器上备份网络服务提供商服务器的内容。

9. 发明申请

US20060188912A1 Sample preparation method incorporating an alkaline shock 有权
公开(公告)号：US20060188912A1
公开(公告)日：2006-08-24
申请号：US11356613
申请日：2006-02-17
申请人： Kui Gao , Michael Becker , Wen Wu , Jeffrey Linnen
发明人： Kui Gao , Michael Becker , Wen Wu , Jeffrey Linnen
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1013 , C12Q1/6806 , C12Q1/689 , C12Q2527/119
摘要： Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.

10. 发明授权

US08385432B2 Method and apparatus for encoding video data, and method and apparatus for decoding video data 有权
公开(公告)号：US08385432B2
公开(公告)日：2013-02-26
申请号：US11920819
申请日：2006-05-03
申请人： Ying Chen , Jiefu Zhai , Kui Gao , Kai Xie
发明人： Ying Chen , Jiefu Zhai , Kui Gao , Kai Xie
IPC分类号： H04N7/12
CPC分类号： H04N19/615 , H04N19/103 , H04N19/105 , H04N19/13 , H04N19/159 , H04N19/172 , H04N19/33 , H04N19/59 , H04N19/61 , H04N19/63
摘要： Scalable video coding based on H.264/AVC uses motion compensation, which results in motion vectors and residual pictures. To optimize the encoding of the residual pictures of the enhancement-layer, they can be predicted from the base-layer. This requires complex encoders and decoders. Simplified residual prediction techniques are provided that focus on reducing the encoding and decoding complexity of the spatial scalable enhancement-layer with optimized coding efficiency. The method for encoding video data containing high-pass frames and low-pass frames comprises the steps of encoding the low-pass frames, wherein residual prediction may be used, splitting the high-pass frames into two interleaving frame groups, encoding the frames of the first of said frame groups, wherein residual prediction may be used, and encoding the frames of the second of the frame groups using an encoding method without residual prediction.

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