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    • 3. 发明申请
    • Chlorophyllous totipotent cell cultures
    • 叶绿素全能细胞培养
    • US20050155119A1
    • 2005-07-14
    • US11031896
    • 2005-01-07
    • Pon JayakumarNicole HopkinsJoseph PetolinoDayakar Pareddy
    • Pon JayakumarNicole HopkinsJoseph PetolinoDayakar Pareddy
    • A01H4/00C12N5/00C12N5/04C12N15/82A01H1/00A01H5/00
    • A01H4/008A01H4/005C12N5/0025C12N5/04C12N15/8201C12N15/8214
    • The subject invention provides totipotent, chlorophyllous, cell cultures of maize. In addition, the methods of producing such cultures are applicable to other related species, including cereals such as rice, oats, barley, and heat. The subject cultures are valuable for herbicide studies, studies for enhancing photosynthesis, and genetic manipulation, such as plastid transformation. The methods of the subject invention are capable of providing high percentages of totipotent cells. These cells are capable of sustained cell division and are competent for regeneration over long periods; they provide high-quality target tissue for nuclear and organelle transformation. The invention also describes methods for the introduction of heterologous DNA into the chloroplast genome. The present invention also provides methods, vectors, and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues.
    • 本发明提供了全能的,叶绿素,玉米的细胞培养物。 此外,生产这种培养物的方法适用于其他相关物种,包括谷物如大米,燕麦,大麦和热。 主题培养物对于除草剂研究,增强光合作用的研究和遗传操作如质体转化是有价值的。 本发明的方法能够提供高百分比的全能细胞。 这些细胞能够持续细胞分裂,并且能够长时间再生; 它们为核和细胞器转化提供高质量的靶组织。 本发明还描述了将异源DNA引入叶绿体基因组的方法。 本发明还提供用于增强转基因植物和植物组织中重组核酸序列表达的方法,载体和基因构建体。