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1. 发明申请

US20090143570A1 METHOD FOR ISOLATION OF GENOMIC DNA, RNA AND PROTEINS FROM A SINGLE SAMPLE 审中-公开
标题翻译：从单个样品中分离基因DNA，RNA和蛋白质的方法
公开(公告)号：US20090143570A1
公开(公告)日：2009-06-04
申请号：US12277420
申请日：2008-11-25
申请人： Miao Jiang , Mark S. Briggs , Rohini Dhulipala , Yuyang Christine Cai , Renee E. Bruno
发明人： Miao Jiang , Mark S. Briggs , Rohini Dhulipala , Yuyang Christine Cai , Renee E. Bruno
IPC分类号： C07K1/16 , C07H21/04 , C07H21/02
CPC分类号： C12N15/1006 , C07K1/34
摘要： The invention provides systems, methods and kits for the separation and/or purification of at least two cellular components selected from genomic DNA, RNA and proteins. The method includes first lysing a biological sample to generate an aqueous solution containing the cellular components; then applying the aqueous solution to a first mineral support under conditions for genomic DNA to bind; and collecting the flowthrough which contains unbound total RNA and proteins. The method further includes applying the flowthrough to a second mineral support under conditions for RNA to bind, and collecting the flowthrough which contains proteins. The genomic DNA and total RNA bound can be eluted while the protein in the flowthrough can be further purified. Further the total RNA isolated could be used to isolate small RNA such as microRNA.
摘要翻译：本发明提供用于分离和/或纯化选自基因组DNA，RNA和蛋白质的至少两种细胞组分的系统，方法和试剂盒。该方法包括首先裂解生物样品以产生含有细胞组分的水溶液; 然后在将基因组DNA结合的条件下将该水溶液施加到第一矿物载体上; 并收集含有未结合的总RNA和蛋白质的流程。该方法还包括在RNA结合的条件下将流出物应用于第二矿物质载体，并收集含有蛋白质的流路。可以洗脱基因组DNA和结合的总RNA，同时可以进一步纯化流过的蛋白质。此外，分离的总RNA可用于分离小RNA如微小RNA。

2. 发明申请

US20110172405A1 METHOD FOR SMALL RNA ISOLATION 审中-公开
标题翻译：小RNA分离方法
公开(公告)号：US20110172405A1
公开(公告)日：2011-07-14
申请号：US13063546
申请日：2009-09-17
申请人： Rohini Dhulipala , Yuyang Christine Cai , Miao Jiang , Mubasher Dar
发明人： Rohini Dhulipala , Yuyang Christine Cai , Miao Jiang , Mubasher Dar
IPC分类号： C07H21/02
CPC分类号： C12N15/111 , C12N15/1006 , C12N2310/141 , C12N2330/10
摘要： This invention relates to a simple and rapid method for the extraction and purification of small RNA from a sample solution. Accordingly, a sample is first mixed with an organic solvent to form a mixture containing the solvent. The mixture is applied to a first mineral support for large RNA to bind. The filtrate is collected which contain unbound small RNA, and is mixed with a second organic solvent to form a second mixture containing the second solvent. This second mixture is applied to a second mineral support for small RNA to bind. After a wash step, the small RNA is eluted. Also provided is a method for the isolation of large RNA, by eluting the large RNA from the first mineral support. In addition, total protein is present in the filtrate and can be isolated by a conventional method.
摘要翻译：本发明涉及从样品溶液中提取和纯化小RNA的简单而快速的方法。因此，首先将样品与有机溶剂混合以形成含有溶剂的混合物。将该混合物应用于第一种矿物载体以用于大RNA结合。收集含有未结合的小RNA的滤液，并与第二有机溶剂混合以形成含有第二溶剂的第二混合物。将该第二混合物应用于第二种矿物载体以用于小RNA结合。洗涤步骤后，小RNA被洗脱。还提供了通过从第一矿物质载体洗脱大RNA来分离大RNA的方法。此外，总蛋白存在于滤液中，可以通过常规方法分离。

3. 发明申请

US20110092692A1 METHOD FOR SEPARATION OF DOUBLE-STRANDED AND SINGLE-STRANDED NUCLEIC ACIDS FROM THE SAME SAMPLE 失效
标题翻译：从同一样品中分离双链和单链核酸的方法
公开(公告)号：US20110092692A1
公开(公告)日：2011-04-21
申请号：US12999325
申请日：2009-06-02
申请人： Miao Jiang
发明人： Miao Jiang
IPC分类号： C07H1/06
CPC分类号： C07H21/02 , C07H21/04 , C12N15/1006
摘要： The invention provides systems, methods and kits for the separation and/or purification of double-stranded and single-stranded nucleic acids from the same sample. The method includes first mixing a sample containing both double-stranded nucleic acid and single-stranded nucleic acid with a solution including a chaotropic salt and a non-ionic detergent to generate a mixture; then applying the mixture to a first mineral support for double-stranded nucleic acid to bind; and collecting the flow-through which contains unbound single-stranded nucleic acid. The method further includes diluting the non-ionic detergent of the flow-through, and applying the diluted flow-through to a second mineral support for the single-stranded nucleic acid to bind. Alternatively the flow-through can be mixed with a lower aliphatic alcohol prior to loading of the second column. The double-stranded and the single-stranded nucleic acids can be eluted from the mineral supports respectively.
摘要翻译：本发明提供用于从相同样品分离和/或纯化双链和单链核酸的系统，方法和试剂盒。该方法包括首先将含有双链核酸和单链核酸的样品与包含离液序列的盐和非离子型洗涤剂的溶液混合以产生混合物; 然后将混合物施加到第一矿物载体上用于双链核酸结合; 并收集含有未结合的单链核酸的流通。该方法还包括稀释流过的非离子洗涤剂，并将稀释的流通通过至第二矿物载体以使单链核酸结合。或者，在加载第二塔之前，流通可与低级脂族醇混合。双链和单链核酸可以分别从矿物载体中洗脱出来。

4. 发明申请

US20110046361A1 METHOD FOR SEPARATION OF DOUBLE-STRANDED AND SINGLE-STRANDED NUCLEIC ACIDS 审中-公开
标题翻译：分离双链和单链核酸的方法
公开(公告)号：US20110046361A1
公开(公告)日：2011-02-24
申请号：US12990242
申请日：2009-04-22
申请人： Miao Jiang
发明人： Miao Jiang
IPC分类号： C07H1/06
CPC分类号： C12N15/101
摘要： The invention provides systems, methods and kits for the separation and/or purification of double-stranded and single-stranded nucleic acids. The method includes first mixing a sample containing the double-stranded nucleic acid and the single-stranded nucleic acid with a pH-neutral, buffered solution consisting essentially of a chaotropic salt and a pH buffer to generate a mixture; then applying the mixture to a first mineral support for the double-stranded nucleic acid to bind; and collecting the flow-through which contains unbound single-stranded nucleic acid. The method further includes adjusting the pH of the flow-through to an acidic pH, and applying the acidified flow-through to a second mineral support for the single-stranded nucleic acid to bind. Alternatively the flow-through can be mixed with a lower aliphatic alcohol prior to loading of the second column. The double-stranded and the single-stranded nucleic acids bound can be eluted from the mineral supports respectively.
摘要翻译：本发明提供用于分离和/或纯化双链和单链核酸的系统，方法和试剂盒。该方法包括首先将含有双链核酸和单链核酸的样品与基本上由离液盐和pH缓冲液组成的pH中性缓冲溶液混合以产生混合物; 然后将混合物施加到第一矿物载体上以使双链核酸结合; 并收集含有未结合的单链核酸的流通。该方法还包括将流过的pH调节至酸性pH，并将酸化的流过物施加到第二矿物载体上以使单链核酸结合。或者，在加载第二塔之前，流通可与低级脂族醇混合。结合的双链和单链核酸可分别从矿物载体洗脱出来。

5. 发明授权

US08648187B2 Method for separation of double-stranded and single-stranded nucleic acids from the same sample 失效
标题翻译：从同一样品分离双链和单链核酸的方法
公开(公告)号：US08648187B2
公开(公告)日：2014-02-11
申请号：US12999325
申请日：2009-06-02
申请人： Miao Jiang
发明人： Miao Jiang
IPC分类号： C07H21/02 , C07H21/04
CPC分类号： C07H21/02 , C07H21/04 , C12N15/1006
摘要： The invention provides systems, methods and kits for the separation and/or purification of double-stranded and single-stranded nucleic acids from the same sample. The method includes first mixing a sample containing both double-stranded nucleic acid and single-stranded nucleic acid with a solution including a chaotropic salt and a non-ionic detergent to generate a mixture; then applying the mixture to a first mineral support for double-stranded nucleic acid to bind; and collecting the flow-through which contains unbound single-stranded nucleic acid. The method further includes diluting the non-ionic detergent of the flow-through, and applying the diluted flow-through to a second mineral support for the single-stranded nucleic acid to bind. Alternatively the flow-through can be mixed with a lower aliphatic alcohol prior to loading of the second column. The double-stranded and the single-stranded nucleic acids can be eluted from the mineral supports respectively.
摘要翻译：本发明提供用于从相同样品分离和/或纯化双链和单链核酸的系统，方法和试剂盒。该方法包括首先将含有双链核酸和单链核酸的样品与包含离液序列的盐和非离子型洗涤剂的溶液混合以产生混合物; 然后将混合物施加到第一矿物载体上用于双链核酸结合; 并收集含有未结合的单链核酸的流通。该方法还包括稀释流过的非离子洗涤剂，并将稀释的流通通过至第二矿物载体以使单链核酸结合。或者，在加载第二塔之前，流通可与低级脂族醇混合。双链和单链核酸可以分别从矿物载体中洗脱出来。

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