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1. 发明申请

US20080118944A1 SEQUENTIAL ANALYSIS OF BIOLOGICAL SAMPLES 有权
标题翻译：生物样品的序列分析
公开(公告)号：US20080118944A1
公开(公告)日：2008-05-22
申请号：US11864093
申请日：2007-09-28
申请人： Melinda Larsen , Anup Sood , Michael J. Gerdes , Michael Christopher Montalto , Zhengyu Pang , Fiona Ginty
发明人： Melinda Larsen , Anup Sood , Michael J. Gerdes , Michael Christopher Montalto , Zhengyu Pang , Fiona Ginty
IPC分类号： C12Q1/02 , G01N21/76 , G01N33/00 , G01N33/68
CPC分类号： G01N33/53 , C12Q1/68 , G01N33/542 , G01N33/582 , Y10T436/143333
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets adhered to a solid support, binding at least one fluorescent probe to one or more target present in the sample, and observing a signal from the fluorescent probe. The method further includes the steps of oxidizing the bound fluorescent probe with a solution containing an oxidizing agent that substantially inactivates the fluorescent probe, binding at least one fluorescent probe to one or more target present in the sample, and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译：提供了探测生物样品中多个靶标的方法。所述方法包括以下步骤：提供包含粘附到固体支持物上的多个靶的生物样品，将至少一种荧光探针结合到样品中存在的一种或多种靶，并观察来自荧光探针的信号。所述方法还包括以下步骤：用含有基本上灭活荧光探针的氧化剂的溶液氧化结合的荧光探针，将至少一种荧光探针结合到样品中存在的一种或多种靶，并观察来自荧光探针的信号。本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。还提供了相关的套件。

2. 发明授权

US07741046B2 Sequential analysis of biological samples 有权
标题翻译：生物样品的顺序分析
公开(公告)号：US07741046B2
公开(公告)日：2010-06-22
申请号：US11864093
申请日：2007-09-28
申请人： Melinda Larsen , Anup Sood , Michael J. Gerdes , Michael Christopher Montalto , Zhengyu Pang , Fiona Ginty
发明人： Melinda Larsen , Anup Sood , Michael J. Gerdes , Michael Christopher Montalto , Zhengyu Pang , Fiona Ginty
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： G01N33/53 , C12Q1/68 , G01N33/542 , G01N33/582 , Y10T436/143333
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets adhered to a solid support, binding at least one fluorescent probe to one or more target present in the sample, and observing a signal from the fluorescent probe. The method further includes the steps of oxidizing the bound fluorescent probe with a solution containing an oxidizing agent that substantially inactivates the fluorescent probe, binding at least one fluorescent probe to one or more target present in the sample, and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译：提供了探测生物样品中多个靶标的方法。所述方法包括以下步骤：提供包含粘附到固体支持物上的多个靶的生物样品，将至少一种荧光探针结合到样品中存在的一种或多种靶，并观察来自荧光探针的信号。所述方法还包括以下步骤：用含有基本上灭活荧光探针的氧化剂的溶液氧化结合的荧光探针，将至少一种荧光探针结合到样品中存在的一种或多种靶，并观察来自荧光探针的信号。本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。还提供了相关的套件。

3. 发明授权

US07741045B2 Sequential analysis of biological samples 有权
标题翻译：生物样品的顺序分析
公开(公告)号：US07741045B2
公开(公告)日：2010-06-22
申请号：US11864085
申请日：2007-09-28
申请人： Michael J. Gerdes , Anup Sood , Michael Christopher Montalto , Ali Can , Fiona Ginty , Maureen Ann Bresnahan , Robert John Filkins , Zhengyu Pang
发明人： Michael J. Gerdes , Anup Sood , Michael Christopher Montalto , Ali Can , Fiona Ginty , Maureen Ann Bresnahan , Robert John Filkins , Zhengyu Pang
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： G01N33/582 , C12Q1/6816 , G01N33/542 , C12Q2545/101 , C12Q2527/125
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one fluorescent probe to one or more target present in the sample, and binding at least at least one control probe to one or more target present in the sample. The methods include the steps of observing a signal from the fluorescent probe and a control signal from the control probe and applying to the sample a basic solution containing an oxidizing agent that selectively inactivates the fluorescent probe and not the control probe. The methods further include the steps of binding at least one fluorescent probe to one or more target present in the sample and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译：提供了探测生物样品中多个靶标的方法。所述方法包括以下步骤：提供含有多个靶标的生物样品，将至少一种荧光探针结合至样品中存在的一种或多种靶标，以及将至少一种对照探针结合至样品中存在的一种或多种靶标。所述方法包括以下步骤：观察来自荧光探针的信号和来自对照探针的控制信号，并向样品施加含有选择性灭活荧光探针而不是对照探针的氧化剂的碱性溶液。所述方法还包括将至少一种荧光探针结合到样品中存在的一种或多种目标物并观察来自荧光探针的信号的步骤。本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。还提供了相关的套件。

4. 发明申请

US20080118934A1 SEQUENTIAL ANALYSIS OF BIOLOGICAL SAMPLES 有权
标题翻译：生物样品的序列分析
公开(公告)号：US20080118934A1
公开(公告)日：2008-05-22
申请号：US11864085
申请日：2007-09-28
申请人： Michael J. Gerdes , Anup Sood , Michael Christopher Montalto , Ali Can , Fiona Ginty , Maureen Ann Bresnahan , Robert John Filkins , Zhengyu Pang
发明人： Michael J. Gerdes , Anup Sood , Michael Christopher Montalto , Ali Can , Fiona Ginty , Maureen Ann Bresnahan , Robert John Filkins , Zhengyu Pang
IPC分类号： G01N33/53 , G01N33/566 , G01N33/567
CPC分类号： G01N33/582 , C12Q1/6816 , G01N33/542 , C12Q2545/101 , C12Q2527/125
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one fluorescent probe to one or more target present in the sample, and binding at least at least one control probe to one or more target present in the sample. The methods include the steps of observing a signal from the fluorescent probe and a control signal from the control probe and applying to the sample a basic solution containing an oxidizing agent that selectively inactivates the fluorescent probe and not the control probe. The methods further include the steps of binding at least one fluorescent probe to one or more target present in the sample and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译：提供了探测生物样品中多个靶标的方法。所述方法包括以下步骤：提供含有多个靶标的生物样品，将至少一种荧光探针结合至样品中存在的一种或多种靶标，以及将至少一种对照探针结合至样品中存在的一种或多种靶标。所述方法包括以下步骤：观察来自荧光探针的信号和来自对照探针的控制信号，并向样品施加含有选择性灭活荧光探针而不是对照探针的氧化剂的碱性溶液。所述方法还包括将至少一种荧光探针结合到样品中存在的一种或多种目标物并观察来自荧光探针的信号的步骤。本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。还提供了相关的套件。

5. 发明申请

US20120252685A1 SEQUENTIAL ANALYSIS OF BIOLOGICAL SAMPLES 有权
标题翻译：生物样品的序列分析
公开(公告)号：US20120252685A1
公开(公告)日：2012-10-04
申请号：US11864098
申请日：2007-09-28
申请人： Thomas Pirrie Treynor , Anup Sood , Michael J. Gerdes , Zhengyu Pang
发明人： Thomas Pirrie Treynor , Anup Sood , Michael J. Gerdes , Zhengyu Pang
IPC分类号： C40B30/04 , C40B40/10 , C40B40/06 , G01N21/64
CPC分类号： G01N33/581 , G01N33/6803
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a sample containing multiple targets, binding at least one probe having a binder coupled to an enzyme to one or more target present in the sample, and reacting the bound probe with an enzyme substrate coupled to a fluorescent signal generator. The methods include the steps of observing a signal from the fluorescent signal generator and applying to the sample a solution containing an oxidizing agent that substantially inactivates both the fluorescent signal generator and the enzyme. The methods further include the steps of binding at least one probe having a binder coupled to an enzyme to one or more target present in the sample of step, reacting the bound probe with an enzyme substrate coupled to a fluorescent signal generator; and observing a signal from the fluorescent signal generator. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译：提供了探测生物样品中多个靶标的方法。所述方法包括以下步骤：提供含有多个靶标的样品，将至少一种具有与酶偶联的结合剂的探针结合至样品中存在的一种或多种靶标，以及使结合的探针与偶联于荧光信号发生器。所述方法包括以下步骤：观察来自荧光信号发生器的信号，并向样品施加含有基本上灭活荧光信号发生器和酶的氧化剂的溶液。所述方法还包括以下步骤：将具有与酶偶联的粘合剂的至少一种探针与步骤样品中存在的一种或多种靶结合，使结合的探针与偶联于荧光信号发生器的酶底物反应; 并观察来自荧光信号发生器的信号。本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。还提供了相关的套件。

6. 发明授权

US08305579B2 Sequential analysis of biological samples 有权
标题翻译：生物样品的顺序分析
公开(公告)号：US08305579B2
公开(公告)日：2012-11-06
申请号：US11864098
申请日：2007-09-28
申请人： Thomas Pirrie Treynor , Anup Sood , Michael J. Gerdes , Zhengyu Pang
发明人： Thomas Pirrie Treynor , Anup Sood , Michael J. Gerdes , Zhengyu Pang
IPC分类号： G01N21/85
CPC分类号： G01N33/581 , G01N33/6803
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a sample containing multiple targets, binding at least one probe having a binder coupled to an enzyme to one or more target present in the sample, and reacting the bound probe with an enzyme substrate coupled to a fluorescent signal generator. The methods include the steps of observing a signal from the fluorescent signal generator and applying to the sample a solution containing an oxidizing agent that substantially inactivates both the fluorescent signal generator and the enzyme. The methods further include the steps of binding at least one probe having a binder coupled to an enzyme to one or more target present in the sample of step, reacting the bound probe with an enzyme substrate coupled to a fluorescent signal generator; and observing a signal from the fluorescent signal generator. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译：提供了探测生物样品中多个靶标的方法。所述方法包括以下步骤：提供含有多个靶标的样品，将至少一种具有与酶偶联的结合剂的探针结合至样品中存在的一种或多种靶标，以及使结合的探针与偶联于荧光信号发生器。所述方法包括以下步骤：观察来自荧光信号发生器的信号，并向样品施加含有基本上灭活荧光信号发生器和酶的氧化剂的溶液。所述方法还包括以下步骤：将具有与酶偶联的粘合剂的至少一种探针与步骤样品中存在的一种或多种靶结合，使结合的探针与偶联于荧光信号发生器的酶底物反应; 并观察来自荧光信号发生器的信号。本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。还提供了相关的套件。

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