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    • 2. 发明专利
    • Method for analysis of anthocyanin
    • 蒽醌的分析方法
    • JP2010236905A
    • 2010-10-21
    • JP2009082557
    • 2009-03-30
    • Meiji Seika Kaisha Ltd明治製菓株式会社
    • YONEKURA KUMIKOKOGA JINICHIRO
    • G01N30/26G01N30/74G01N30/88
    • PROBLEM TO BE SOLVED: To provide a method of determining anthocyanin by using an organic solvent in place of acetonitrile as a moving phase, in analysis of the anthocyanin by HPLC.
      SOLUTION: In the method wherein the anthocyanin included in an analysis sample is determined by being separated by high-performance liquid chromatography (HPLC), the moving phase is ethanol and/or water. In the method, for example, a material having a filler (ODS) formed by chemically bonding an octadecyl group to a silica gel carrier is used a column for use in HPLC, and a detection wavelength of the anthocyanin is set at 520 nm, and the anthocyanin included in a sample such as delphinidin-3-O-glucoside, delphinidin-3-O-rutinoside, cyanidin-3-O-glucoside, or cyanidin-3-O-rutinoside can be quantitatively analyzed simply.
      COPYRIGHT: (C)2011,JPO&INPIT
    • 待解决的问题:为了提供通过使用有机溶剂代替乙腈作为移动相来测定花青素的方法,通过HPLC分析花青素。 解决方案:在通过高效液相色谱(HPLC)分离分析分析样品中的花青素的方法中,移动相是乙醇和/或水。 在该方法中,例如使用具有通过将十八烷基化学键合到硅胶载体上而形成的填料(ODS)的材料,用于HPLC中的色谱柱,花青素的检测波长设定在520nm,并且 可以简单地定量分析样品中包含的花青素如花翠素-3-O-葡萄糖苷,花翠素-3-O-芸香苷,花青素-3-O-葡萄糖苷或花青素-3-O-芸香苷。 版权所有(C)2011,JPO&INPIT