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    • 1. 发明申请
    • DNA SEQUENCE THAT CONFERS APHID RESISTANCE IN SOYBEAN
    • DNA序列在大豆中构成抗性
    • US20130198912A1
    • 2013-08-01
    • US13577227
    • 2011-02-04
    • Matthew HudsonBrian DiersKi-Seung KimJianping Wang
    • Matthew HudsonBrian DiersKi-Seung KimJianping Wang
    • C12Q1/68
    • C12Q1/6895C07K14/415C12N15/8286Y02A40/162
    • Provided herein are isolated nucleic acid molecules representing a genetically defined region of the genome of the aphid resistant soybean plant (Glycine max) cultivar Dowling that confers resistance to soybean aphid (Aphis glycines). Within the region is a gene encoding the aphid resistance protein Rag1. Rag1 aphid resistance amino acid sequences are also provided. Also provided herein are methods for conferring aphid resistance on a plant or enhancing aphid resistance in a plant by transforming it to contain and express such nucleic acid sequences encoding Rag1 aphid resistance or introgressing DNA encoding the trait into the plant by plant breeding. Further provided are polymorphic markers useful for identifying plant germplasm containing aphid resistance, and methods for makings such markers.
    • 本文提供了代表赋予对大豆蚜虫(蚜虫甘氨酸)抗性的蚜虫抗性大豆植物(Glycine max)栽培品种Dowling的基因组遗传定义区域的分离的核酸分子。 该区域内是编码蚜虫抗性蛋白Rag1的基因。 还提供了Rag1蚜虫抗性氨基酸序列。 本文还提供了赋予植物蚜虫抗性或增强植物蚜虫抗性的方法,方法是将其转化成含有并表达编码Rag1蚜虫抗性的核酸序列或通过植物育种将编码该性状的DNA插入植物。 还提供了可用于鉴定含有蚜虫抗性的植物种质的多态性标记,以及用于制备这种标记的方法。