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1. 发明授权

US06399305B1 Protection of partial complementary nucleic acid fragment using a electroconductive chip and intercalator 有权
标题翻译：使用导电芯片和嵌入剂保护部分互补核酸片段
公开(公告)号：US06399305B1
公开(公告)日：2002-06-04
申请号：US09588950
申请日：2000-06-07
申请人： Yoshihiko Makino , Yoshihiko Abe , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita , Masashi Ogawa
发明人： Yoshihiko Makino , Yoshihiko Abe , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita , Masashi Ogawa
IPC分类号： C12Q168
CPC分类号： C12Q1/6825 , Y10T436/143333 , C12Q2563/173 , C12Q2563/113
摘要： A method of analyzing a nucleic acid fragment sample to judge whether the nucleic acid fragment sample is uncomplementary, partly complementary or complementary to a DNA fragment in its specific base sequence is conducted by the steps of: bringing an aqueous solution of the nucleic acid fragment sample into contact with a DNA chip having an electroconductive substrate and the DNA fragment fixed onto the substrate in the presence of an electrochemical thread intercalator; measuring an electric current flowing from or to the electroconductive substrate along the DNA fragment under application of a potential to the substrate; and comparing the electric current measured above with a referential electric current which is prepared employing a DNA chip equivalent to the above DNA chip, the intercalator, and an aqueous solution of a nucleic acid fragment which is complementary to the DNA fragment of the DNA chip.
摘要翻译：分析核酸片段样品以判断核酸片段样品是否与其特异性碱基序列中的DNA片段不互补，部分互补或互补的方法通过以下步骤进行：使核酸片段样品的水溶液与具有导电性基体的DNA芯片接触，并且在电化学线性嵌入剂的存在下固定在基材上的DNA片段;测量在施加电位下沿着DNA片段向基片流向或流到导电基底的电流 ; 并使用与上述DNA芯片相当的DNA芯片，嵌入剂和与DNA芯片的DNA片段互补的核酸片段的水溶液制备的参考电流来比较上述电流。

2. 发明授权

US06261780B1 Quantitative analysis of biochemical compound utilizing electrochemical reaction 失效
标题翻译：利用电化学反应定量分析生化化合物
公开(公告)号：US06261780B1
公开(公告)日：2001-07-17
申请号：US09477973
申请日：2000-01-05
申请人： Masashi Ogawa , Shigeori Takenaka , Makoto Takagi
发明人： Masashi Ogawa , Shigeori Takenaka , Makoto Takagi
IPC分类号： C12Q168
CPC分类号： C12Q1/60 , C12Q1/001 , C12Q1/26 , C12Q1/54 , C12Q1/6825 , C12Q2563/173 , C12Q2545/114 , C12Q2527/137
摘要： A method for quantitatively analyzing a biochemical compound such as glucose or cholesterol is performed by bringing the biochemical compound into contact with double stranded DNA fragments which are fixed onto a surface of an electrode at their one terminals and in which electrochemically active threading intercalators are intercalated, in an aqueous medium under application of electric potential to the electrode in the presence of an oxidase capable of oxidizing the biochemical compound and becoming a reduced oxidase, and detecting an electric current flowing between the electrode and another electrode placed in the aqueous medium.
摘要翻译：通过使生物化学化合物与固定在电极表面上的双链DNA片段接触并进行电化学活性穿插嵌入剂的插入，进行定量分析生物化学化合物如葡萄糖或胆固醇的方法，在能够氧化生物化学化合物并变成还原氧化酶的氧化酶存在下向电极施加电位的水性介质中，并且检测在电极和置于水性介质中的另一电极之间流动的电流。

3. 发明授权

US06864055B2 Method for detecting nucleic acids 有权
标题翻译：检测核酸的方法
公开(公告)号：US06864055B2
公开(公告)日：2005-03-08
申请号：US09887625
申请日：2001-06-22
申请人： Yoshihiko Makino , Yoshihiko Abe , Masashi Ogawa , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita
发明人： Yoshihiko Makino , Yoshihiko Abe , Masashi Ogawa , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita
IPC分类号： G01N33/53 , C07B61/00 , C07K14/00 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N27/416 , G01N33/566 , B01D57/02 , C12P19/34 , G01N30/02
CPC分类号： C12Q1/6825 , C07K14/003 , C12Q1/6837 , C40B40/00 , C12Q2565/501 , C12Q2565/102 , C12Q2565/607
摘要： A method of detecting nucleic acid fragments in plural samples is performed by the steps of: attaching an electroconductive label to nucleic acid fragments in one sample and attaching a different electroconductive label to nucleic acid fragments in another sample; preparing a mixture of these samples; spotting the mixture on an electroconductive microarray having plural electrodes onto which probe molecules complementary to the nucleic acid fragments are fixed, so that hybridization between the nucleic acid fragments and the probe molecules on the electroconductive microarray can proceed to form hybrid structures; applying to the electrode an electric potential corresponding to the oxidation-reduction potential of the former label and detecting on the electrode an electric current; applying to the electrode an electric potential corresponding to the oxidation-reduction potential of the latter label and detecting on the electrode an electric current; and comparing the electric current detected in the former detecting procedure and that detected in the latter detecting procedure.
摘要翻译：通过以下步骤进行检测多个样品中的核酸片段的方法：将导电标记物附着在一个样品中的核酸片段上，并将不同的导电标记连接到另一个样品中的核酸片段; 准备这些样品的混合物; 在具有多个电极的导电微阵列上将该混合物点样，在该导电微阵列上固定有与核酸片段互补的探针分子，使得导电微阵列上的核酸片段和探针分子之间的杂交可以进行以形成杂化结构; 向所述电极施加与所述前标签的氧化还原电位相对应的电位，并在所述电极上检测电流; 向所述电极施加与后一标签的氧化还原电位相对应的电位，并在所述电极上检测电流; 并比较前一检测程序检测的电流和后一检测程序中检测的电流。

4. 发明授权

US06713255B1 DNA chip, PNA chip, and their preparation methods 有权
标题翻译： DNA芯片，PNA芯片及其制备方法
公开(公告)号：US06713255B1
公开(公告)日：2004-03-30
申请号：US09589221
申请日：2000-06-07
申请人： Yoshihiko Makino , Yoshihiko Abe , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita , Masashi Ogawa
发明人： Yoshihiko Makino , Yoshihiko Abe , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita , Masashi Ogawa
IPC分类号： C12Q168
CPC分类号： C07K14/003 , B01J19/0046 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00722 , B01J2219/00729 , C07B2200/11 , C07H21/00 , C12Q1/6837 , C40B40/06 , C12Q2565/607
摘要： A DNA chip (or PNA chip) composed of a solid carrier and plural DNA fragments (or PNA fragments) fixed onto is the solid carrier at each one end, wherein a plurality of short chain spacer molecules having a hydrophilic moiety at each one end are fixed at each another end onto a surface of the solid carrier having no DNA fragments (or no PNA fragments) on its surface is effective for high sensitive quantitative analysis of a nucleic acid fragment complementary to the DNA fragment (or PNA fragment).
摘要翻译：由固体载体和固定在其上的多个DNA片段（或PNA片段）组成的DNA芯片（或PNA芯片）是每一端的固体载体，其中在每一端具有亲水部分的多个短链间隔基分子是固定在其表面上没有DNA片段（或没有PNA片段）的固体载体的表面上对于与DNA片段（或PNA片段）互补的核酸片段的高敏感性定量分析是有效的。

5. 发明授权

US06506567B2 Water-soluble flourescent intercalator compound 有权
标题翻译：水溶性荧光嵌入剂化合物
公开(公告)号：US06506567B2
公开(公告)日：2003-01-14
申请号：US09774895
申请日：2001-01-31
申请人： Yoshihiko Makino , Masashi Ogawa , Makoto Takagi , Shigeori Takenaka
发明人： Yoshihiko Makino , Masashi Ogawa , Makoto Takagi , Shigeori Takenaka
IPC分类号： C12Q168
CPC分类号： C07D471/06 , C12Q1/6816 , Y10T436/143333 , C12Q2563/173
摘要： A water-soluble fluorescent intercalator compound having the formula: F—La—X F is a fluorescent moiety, X is a divalent cyclic group, and La is a linking group, and at least one of X and La has a site imparting water solubility to the compound or a site that is convertible into a site imparting water solubility to the compound is favorably employable as a fluorescent intercalator in a method for fluorometrically detecting complementary DNA fragments.
摘要翻译：具有式：F的水溶性荧光嵌入剂化合物是荧光部分，X是二价环状基团，La是连接基团，X和La中的至少一个具有赋予化合物水溶性的部位或可以转化为赋予化合物水溶性的部位的部位在荧光测定互补DNA片段的方法中有利地用作荧光嵌入剂。

6. 发明授权

US06368807B2 Threading intercalator having oxidation-reduction activity 有权
标题翻译：具有氧化还原活性的螺纹嵌入剂
公开(公告)号：US06368807B2
公开(公告)日：2002-04-09
申请号：US09733365
申请日：2000-12-08
申请人： Yoshihiko Makino , Kazunobu Takahashi , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita
发明人： Yoshihiko Makino , Kazunobu Takahashi , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita
IPC分类号： C12Q168
CPC分类号： C07F17/02
摘要： A compound of the following formula: Ea—La—X—Lb—Eb in which each of Ea and Eb independently is a group having oxidation-reduction activity and having a conjugated system in its group; X is a divalent cyclic group; and each of La and Lb independently is a group which does not form a conjugated system in combination with the conjugated system of each of Ea and Eb and at least one of which has a site imparting water solubility to the compound or a site that is convertible into a site imparting water solubility to the compound, is favorably employable as an electroconductive threading intercalator in an electrochemical method for detecting complementary DNA fragments.
摘要翻译：下式的化合物：其中Ea和Eb各自独立地是具有氧化还原活性的基团并且在其组中具有共轭体系; X是二价环状基团; 独立地，La和Lb各自是不与Ea和Eb各自的共轭体系结合形成共轭体系的基团，并且其中至少一个具有赋予化合物或可转化位点水溶性的位点成为赋予化合物水溶性的部位有利地用作检测互补DNA片段的电化学方法中的导电穿线嵌入剂。

7. 发明申请

US20120163124A1 ULTRASONIC DETECTION DEVICE AND ULTRASONIC DIAGNOSTIC DEVICE 有权
标题翻译：超声检测装置和超声诊断装置
公开(公告)号：US20120163124A1
公开(公告)日：2012-06-28
申请号：US13393918
申请日：2010-10-28
申请人： Takahiro Akiyama , Makoto Takagi , Kazunari Fujii , Hidemasa Mizutani
发明人： Takahiro Akiyama , Makoto Takagi , Kazunari Fujii , Hidemasa Mizutani
IPC分类号： G01N29/24 , B06B1/02
CPC分类号： G01N29/2418 , B06B1/0292 , G01N29/2406 , G01N2291/101
摘要： Provided is an ultrasonic detection device including: a capacitive electromechanical transducer including a cell that includes a first electrode and a second electrode disposed so as to oppose with a space; a voltage source for developing a potential difference between the first electrode and the second electrode; and an electric circuit for converting a current, which is caused by a change in electrostatic capacitance between the first electrode and the second electrode due to vibration of the second electrode, into a voltage, in which the capacitive electromechanical transducer provides an output current with a high-pass characteristic having a first cutoff frequency with respect to a frequency, the electric circuit provides an output with a low-pass characteristic having a second cutoff frequency with respect to the frequency, and the second cutoff frequency is smaller than the first cutoff frequency.
摘要翻译：本发明提供一种超声波检测装置，其特征在于，包括：电容式机电换能器，其包括具有与空间对置配置的第一电极和第二电极的电池单元; 用于在第一电极和第二电极之间形成电位差的电压源; 以及用于将由于第二电极的振动而由第一电极和第二电极之间的静电电容的变化引起的电流转换成电压的电路，其中电容式机电换能器提供输出电流具有相对于频率具有第一截止频率的高通特性，所述电路提供具有相对于所述频率具有第二截止频率的低通特性的输出，并且所述第二截止频率小于所述第一截止频率。

8. 发明申请

US20100213791A1 OSCILLATOR DEVICE 有权
标题翻译：振荡器装置
公开(公告)号：US20100213791A1
公开(公告)日：2010-08-26
申请号：US12599688
申请日：2008-06-30
申请人： Atsushi Kandori , Chienliu Chang , Makoto Takagi
发明人： Atsushi Kandori , Chienliu Chang , Makoto Takagi
IPC分类号： H02N1/00 , H03B1/00
CPC分类号： B81B3/0086 , B81B2201/0242 , B81B2201/033 , B81B2203/058 , G01C19/5719
摘要： An oscillator device that includes a movable body oscillatably supported about a rotation axis, wherein the movable body is separated into plural electrically separated conductive regions in the thickness direction, and at least one of the plural electrically separated conductive regions in the thickness direction further has plural electrically separated conductive regions.
摘要翻译：一种振荡器装置，包括围绕旋转轴可摆动地支撑的可移动体，其中所述可移动体在所述厚度方向上被分离为多个分离的导电区域，并且所述多个电分离的导电区域中的至少一个在所述厚度方向上还具有多个电分离的导电区域。

9. 发明授权

US07379089B2 Apparatus and method for monitoring the immediate surroundings of a vehicle 失效
标题翻译：用于监测车辆的周围环境的装置和方法
公开(公告)号：US07379089B2
公开(公告)日：2008-05-27
申请号：US10732315
申请日：2003-12-11
申请人： Makoto Takagi , Motonori Tanaka
发明人： Makoto Takagi , Motonori Tanaka
IPC分类号： H04N7/18
CPC分类号： H04N7/181 , B60R11/04 , B60R2011/004
摘要： An apparatus for monitoring the immediate surroundings of a vehicle displays on a display within the vehicle cabin a captured image of the vehicle immediate surroundings captured by a camera mounted on a back door which can open and close. The apparatus determines whether the back door is open based on a signal output from a back door courtesy switch. If it is determined that the back door is open, a written notification that the back door is open is superimposed on the display showing the captured image from the camera. If it is determined that the back door is not open, the written notification is not shown.
摘要翻译：用于监测车辆的周围环境的装置在车厢内的显示器上显示由安装在可打开和关闭的后门上的照相机捕获的车辆立即环境的捕获图像。该装置基于从后门礼貌开关输出的信号来确定后门是否打开。如果确定后门打开，则将从后门打开的书面通知叠加在显示器上的相机上显示拍摄图像。如果确定后门未打开，则未显示书面通知。

10. 发明申请

US20070236563A1 Vehicle periphery monitor 审中-公开
公开(公告)号：US20070236563A1
公开(公告)日：2007-10-11
申请号：US11806764
申请日：2007-06-04
申请人： Makoto Takagi , Toshiyasu Katsuno , Kazushi Konno , Toshiaki Kakinami
发明人： Makoto Takagi , Toshiyasu Katsuno , Kazushi Konno , Toshiaki Kakinami
IPC分类号： H04N7/18
CPC分类号： H04N7/18 , B60R1/00 , B60R2300/105 , B60R2300/302 , B60R2300/70
摘要： A front camera for filming an area stretching forwards is disposed in a front portion of a vehicle. A side camera for filming an area stretching laterally and diagonally forwards is disposed in a lateral-left portion of the vehicle. An indicator display that can be visually recognized by a driver is disposed in a compartment of the vehicle. An amount of change in the direction of the vehicle, that is, a deflection angle of the vehicle is detected on the basis of a vehicle speed and a steering angle of a steering wheel, which have been detected by means of various sensors. After the indicator display has started displaying a lateral photo image filmed by the side camera, the photo image displayed by the indicator display is shifted from the lateral photo image filmed by the side camera to a front photo image filmed by the front camera if the deflection angle has reached a predetermined angle.

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