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    • 10. 发明申请
    • Apparatus for Systematic Single Cell Tracking of Distinctive Cellular Events
    • 用于系统性单细胞跟踪特异性细胞事件的装置
    • US20130217061A1
    • 2013-08-22
    • US13880793
    • 2011-10-07
    • Masahiko SatoSachiko Sato
    • Masahiko SatoSachiko Sato
    • C12Q1/02
    • G01N33/5091C12M41/46C12Q1/02G01N33/5017G02B21/367
    • An apparatus for quantitative identification of distinctive cellular events occurring in a cell population using a non-fluorescence approach. The apparatus comprises an image acquisition unit having a Differential Interference Contrast microscope with a camera, a light source, an environmental chamber allowing carrying out cell culture of at least one cell in a cell population; the image acquisition unit acquiring images of the cell population, at predetermined time points; a cell tracker for individually tracking the at least one cell of the cell population in the images; a distinctive cellular event detector for detecting an occurrence of a distinctive cellular event; a report generator; wherein the distinctive cellular event is selected from the group consisting of: tripolar, tetrapolar, quadpolar cell division, cell fusion, cell death, impaired cell division, cell shape alteration, nuclear shape alteration, inner cellular material accumulation, cell enlargement, engulfing, hyper-mobilization, hypo-mobilization and prolonged doubling time.
    • 用于使用非荧光方法定量鉴定发生在细胞群体中的特异性细胞事件的装置。 该装置包括具有相机的差分干涉对比显微镜的图像获取单元,光源,允许在细胞群体中进行至少一个细胞的细胞培养的环境室; 所述图像获取单元在预定时间点获取所述细胞群体的图像; 用于单独跟踪图像中的细胞群体的至少一个细胞的细胞跟踪器; 用于检测特征性细胞事件的发生的特征性细胞事件检测器; 报告生成器 其中特征性细胞事件选自:三极,四极,四极细胞分裂,细胞融合,细胞死亡,细胞分裂受损,细胞形状改变,核形态改变,内细胞物质积累,细胞增殖,吞噬,超 - 动员不足,延长倍增时间。