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1. 发明授权

US5707808A Optical selection and collection of DNA fragments 失效
公开(公告)号：US5707808A
公开(公告)日：1998-01-13
申请号：US632743
申请日：1996-04-15
申请人： Mary C. Roslaniec , John C. Martin , James H. Jett , L. Scott Cram
发明人： Mary C. Roslaniec , John C. Martin , James H. Jett , L. Scott Cram
IPC分类号： C12N15/10 , C12N15/12 , C12Q1/68 , G01N15/14 , G01N21/64
CPC分类号： G01N15/1459 , C12N15/1006 , C12Q1/68 , G01N2015/0038 , G01N2015/149 , Y10T436/143333
摘要： Optical selection and collection of DNA fragments. The present invention includes the optical selection and collection of large (>.mu.g) quantities of clonable, chromosome-specific DNA from a sample of chromosomes. Chromosome selection is based on selective, irreversible photoinactivation of unwanted chromosomal DNA. Although more general procedures may be envisioned, the invention is demonstrated by processing chromosomes in a conventional flow cytometry apparatus, but where no droplets are generated. All chromosomes in the sample are first stained with at least one fluorescent analytic dye and bonded to a photochemically active species which can render chromosomal DNA unclonable if activated. After passing through analyzing light beam(s), unwanted chromosomes are irradiated using light which is absorbed by the photochemically active species, thereby causing photoinactivation. As desired chromosomes pass this photoinactivation point, the inactivating light source is deflected by an optical modulator; hence, desired chromosomes are not photoinactivated and remain clonable. The selection and photoinactivation processes take place on a microsecond timescale. By eliminating droplet formation, chromosome selection rates 50 times greater than those possible with conventional chromosome sorters may be obtained. Thus, usable quantities of clonable DNA from any source thereof may be collected.

2. 发明授权

US5879625A Optical selection and collection of DNA fragments 失效
标题翻译： DNA片段的光学选择和收集
公开(公告)号：US5879625A
公开(公告)日：1999-03-09
申请号：US951955
申请日：1997-10-17
申请人： Mary C. Roslaniec , John C. Martin , James H. Jett , L. Scott Cram
发明人： Mary C. Roslaniec , John C. Martin , James H. Jett , L. Scott Cram
IPC分类号： C12N15/10 , C12N15/12 , C12Q1/68 , G01N15/14 , G01N1/14
CPC分类号： G01N15/1459 , C12N15/1006 , C12Q1/68 , G01N2015/0038 , G01N2015/149 , Y10T436/143333
摘要： Optical selection and collection of DNA fragments. The present invention includes the optical selection and collection of large (>.mu.g) quantities of clonable, chromosome-specific DNA from a sample of chromosomes. Chromosome selection is based on selective, irreversible photoinactivation of unwanted chromosomal DNA. Although more general procedures may be envisioned, the invention is demonstrated by processing chromosomes in a conventional flow cytometry apparatus, but where no droplets are generated. All chromosomes in the sample are first stained with at least one fluorescent analytic dye and bonded to a photochemically active species which can render chromosomal DNA unclonable if activated. After passing through analyzing light beam(s), unwanted chromosomes are irradiated using light which is absorbed by the photochemically active species, thereby causing photoinactivation. As desired chromosomes pass this photoinactivation point, the inactivating light source is deflected by an optical modulator; hence, desired chromosomes are not photoinactivated and remain clonable. The selection and photoinactivation processes take place on a microsecond timescale. By eliminating droplet formation, chromosome selection rates 50 times greater than those possible with conventional chromosome sorters may be obtained. Thus, usable quantities of clonable DNA from any source thereof may be collected.
摘要翻译： DNA片段的光学选择和收集。本发明包括从染色体样品中大量（>μg）量的可克隆，染色体特异性DNA的光学选择和收集。染色体选择基于不需要的染色体DNA的选择性不可逆光激活。尽管可以设想更一般的程序，但是通过在常规流式细胞术装置中处理染色体但是不产生液滴来证明本发明。样品中的所有染色体首先用至少一种荧光分析染料染色，并与光化学活性物质结合，如果激活可使染色体DNA不可克隆。在通过分析光束之后，使用被光化学活性物质吸收的光照射不想要的染色体，从而引起光激活。根据需要，染色体通过该光激活点，灭活光源被光学调制器偏转; 因此，期望的染色体不是光激活的并且保持克隆。选择和光激活过程在微秒的时间尺度上进行。通过消除液滴形成，可以获得比常规染色体分选机可能的染色体选择率高50倍的染色体选择率。因此，可以收集来自其任何来源的可克隆DNA的可用量。

3. 发明授权

US5085673A Compact air scrubber 失效
标题翻译：紧凑型空气洗涤器
公开(公告)号：US5085673A
公开(公告)日：1992-02-04
申请号：US671330
申请日：1991-03-19
申请人： Bill F. Bentley , James H. Jett , John C. Martin , George C. Saunders
发明人： Bill F. Bentley , James H. Jett , John C. Martin , George C. Saunders
IPC分类号： B03C3/014
CPC分类号： B03C3/014
摘要： Method and apparatus for removing material from a gas. A mist created by a piezoelectric ultrasonic transducer is contacted with the gas and both gas and mist are passed through baffled separators. Liquid effluent from the separators contains solid material removed from the gas and gaseous material which reacted with the liquid or was absorbed by the liquid. The invention is useful for collecting a sample of material in a gas, such as a vapor in the atmosphere, and in cleaning a gas. A relatively concentrated solution of a material present in a gas in a very small concentration can be obtained.
摘要翻译：从气体中去除材料的方法和装置。由压电超声换能器产生的雾气与气体接触，气体和雾气均通过挡板分离器。来自分离器的液体流出物包含从与液体反应或被液体吸收的气体和气态物质中去除的固体材料。本发明可用于收集诸如气氛中的气体的气体中的材料样品和清洁气体。可以获得以非常小的浓度存在于气体中的材料的相对浓缩的溶液。

4. 发明授权

US5558998A DNA fragment sizing and sorting by laser-induced fluorescence 失效
标题翻译：通过激光诱导荧光进行DNA片段筛选和分选
公开(公告)号：US5558998A
公开(公告)日：1996-09-24
申请号：US486400
申请日：1995-06-05
申请人： Mark L. Hammond , James H. Jett , Richard A. Keller , Babetta L. Marrone , John C. Martin
发明人： Mark L. Hammond , James H. Jett , Richard A. Keller , Babetta L. Marrone , John C. Martin
IPC分类号： C12Q1/68 , G01N15/14 , G01N33/483 , G01N35/08
CPC分类号： C12Q1/6827 , G01N15/14 , Y10T436/117497
摘要： A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.
摘要翻译：提供了使用高速检测系统（例如流式细胞术）来定尺寸DNA片段的方法，以确定样品中DNA片段的独特特征。在一个表征中，DNA片段在预选位点被片段化以产生多个DNA片段。用有效染色DNA片段或DNA片段化学计量的染料处理DNA片段或得到的DNA片段。然后检查染色片段中来自染料的荧光，以产生功能上与每个DNA片段中的核苷酸数相关的输出。在一个实施方案中，来自每个片段的荧光发射强度与片段长度线性相关。 DNA片段大小的分布形成了用于法医学和研究应用的DNA片段的表征。

5. 发明授权

US5405747A Method for rapid base sequencing in DNA and RNA with two base labeling 失效
标题翻译：用于在两个碱基标记的DNA和RNA中进行快速碱基测序的方法
公开(公告)号：US5405747A
公开(公告)日：1995-04-11
申请号：US208506
申请日：1994-03-07
申请人： James H. Jett , Richard A. Keller , John C. Martin , Richard G. Posner , Babetta L. Marrone , Mark L. Hammond , Daniel J. Simpson
发明人： James H. Jett , Richard A. Keller , John C. Martin , Richard G. Posner , Babetta L. Marrone , Mark L. Hammond , Daniel J. Simpson
IPC分类号： C12Q1/68 , C12P19/34 , G01N33/48
CPC分类号： C12Q1/6816 , C12Q1/6869 , Y10T436/143333
摘要： Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.
摘要翻译：在DNA和RNA中进行快速碱基测序的方法，采用双碱基标记，并采用双波长单分子荧光检测。用于接受荧光标记的碱基用于复制要测序的单个DNA或RNA链。然后从复制的链中顺序地切割碱基，用所选择的电磁辐射谱激发，并且以从链断裂的顺序检测来自单个的标记碱基的荧光。

6. 发明授权

US4962037A Method for rapid base sequencing in DNA and RNA 失效
标题翻译： DNA和RNA快速碱基测序的方法
公开(公告)号：US4962037A
公开(公告)日：1990-10-09
申请号：US105375
申请日：1987-10-07
申请人： James H. Jett , Richard A. Keller , John C. Martin , Robert K. Moyzis , Robert L. Ratliff , E. Brooks Shera , Carleton C. Stewart
发明人： James H. Jett , Richard A. Keller , John C. Martin , Robert K. Moyzis , Robert L. Ratliff , E. Brooks Shera , Carleton C. Stewart
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6813 , C12Q1/6823 , Y10S436/80 , Y10T436/143333 , Y10T436/25125
摘要： A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed.
摘要翻译：提供了用于DNA或RNA片段的快速碱基测序的方法，其中DNA或RNA的单个片段具有可鉴定的碱基并悬浮在移动的流动中。外切核酸酶从悬浮片段的末端依次切割各个碱基。移动流动流将切割的基部保持在有序的列中，用于随后的检测和识别。在一个具体实施方案中，形成DNA或RNA片段的单个碱基用特征荧光染料单独标记。然后将基因列激发成具有各基因的输出光谱特征的荧光。因此，可以重构原始DNA或RNA片段的碱基序列。

7. 发明授权

US4573796A Apparatus for eliminating background interference in fluorescence measurements 失效
标题翻译：用于消除荧光测量中的背景干扰的装置
公开(公告)号：US4573796A
公开(公告)日：1986-03-04
申请号：US568768
申请日：1984-01-06
申请人： John C. Martin , James H. Jett
发明人： John C. Martin , James H. Jett
IPC分类号： G01N15/14 , G01N21/64 , G01J3/42
CPC分类号： G01N21/645 , G01N15/1429 , G01N21/6428 , G01N2015/1438 , G01N2021/6439
摘要： The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle.
摘要翻译：本发明涉及一种用于在多激光流式细胞仪中消除荧光测量期间的背景干扰的装置。用荧光染料染色的生物颗粒被激光激发。荧光检测器检测荧光。颗粒散射光，并产生并延迟栅极信号，直到生物颗粒到达下一个激光。延迟信号打开下一个激光，激发相同生物粒子的不同染色成分。

8. 发明授权

US07731612B2 Arrow launched media reservoir 有权
标题翻译：箭推出媒体库
公开(公告)号：US07731612B2
公开(公告)日：2010-06-08
申请号：US11956023
申请日：2007-12-13
申请人： John C. Martin
发明人： John C. Martin
IPC分类号： F42B6/04
CPC分类号： F42B6/08 , F41J3/0004 , F42B6/02 , F42B12/362 , F42B12/40
摘要： An arrow having an arrowhead assembly attached thereto is provided for marking a remote location with a marking media. The marking media is preferably in the form of a paint ball or a scent ball. The arrowhead assembly includes a media reservoir defining a cavity and a plurality of supports extending outward from the media reservoir along a longitudinal axis. The supports secure the marking media within the cavity in a press-fit connection. In one embodiment, a cap and a resilient nose cone are attached to the media reservoir, surrounding the marking media, to prevent damage to a target upon impacting the target.
摘要翻译：提供了具有附接到其上的箭头组件的箭头，用于用标记介质标记远程位置。标记介质优选为油漆球或气味球的形式。箭头组件包括限定空腔的介质储存器和沿着纵向轴线从介质储存器向外延伸的多个支撑件。支撑件将压痕连接中的标记介质固定在空腔内。在一个实施例中，盖和弹性鼻锥连接到介质储存器，围绕标记介质，以防止在撞击目标时损坏目标物。

9. 发明申请

US20100045232A1 MODULARIZED INTERFACE AND RELATED METHOD FOR CONNECTING PLUG-IN ELECTRIC VEHICLES TO THE ENERGY GRID 有权
标题翻译：将插入式电动车连接到能量网的模块化接口和相关方法
公开(公告)号：US20100045232A1
公开(公告)日：2010-02-25
申请号：US12265678
申请日：2008-11-05
申请人： Chris W. Chen , Robert Peelle , Viral Bhalodia , Ken Yu , Gabriel Gavrielides , Terry Mohn , John C. Martin
发明人： Chris W. Chen , Robert Peelle , Viral Bhalodia , Ken Yu , Gabriel Gavrielides , Terry Mohn , John C. Martin
IPC分类号： H02J7/00
CPC分类号： B60L11/1824 , B60L3/0069 , B60L11/1818 , B60L11/1838 , B60L11/1842 , B60L11/1846 , B60L11/1848 , B60L2230/16 , B60L2270/32 , B60L2270/34 , G06F21/44 , H04L63/08 , Y02E60/721 , Y02T10/7005 , Y02T10/7088 , Y02T90/121 , Y02T90/128 , Y02T90/14 , Y02T90/16 , Y02T90/163 , Y02T90/169 , Y04S10/126 , Y04S30/14
摘要： This invention is directed to a modularized interface for connecting a plug-in electric vehicle to the energy grid. For use with public or semi-public outlets, the modularized interface comprises a module and a smart socket, where the module is integrated within or capable of being connected to, the vehicle's charging interface. The module is normally disabled, but is enabled only after the end user is authenticated, the smart socket and its associated meter have been identified, and the module and the end user's account with the local utility are validated. The module meters the energy consumption, and, when the module is disconnected from the smart socket, indicating termination of the charging session, the metered data is communicated to the utility for updating the end user's account, and the module is disabled. The module is also capable of use with conventional outlets located, for example, in private residences.
摘要翻译：本发明涉及用于将插电式电动车辆连接到能量电网的模块化接口。为了与公共或半公共出口一起使用，模块化接口包括模块和智能插座，其中模块集成在车辆的充电接口内或能够连接到车辆的充电接口。该模块通常被禁用，但仅在最终用户进行身份验证后才能启用，智能插座及其关联的仪表已被识别，模块和最终用户的本地实用程序的帐户将被验证。该模块测量能量消耗，并且当模块与智能插座断开连接时，指示充电会话的终止，将计量数据传送到实用程序以更新最终用户的帐户，并且该模块被禁用。该模块还可以用于例如位于私人住宅中的常规插座。

10. 发明申请

US20080197039A1 FUEL FACTS INDICATOR FOR GASOLINE CONTAINERS 审中-公开
标题翻译：燃油容器指示剂
公开(公告)号：US20080197039A1
公开(公告)日：2008-08-21
申请号：US12030641
申请日：2008-02-13
申请人： John C. Martin
发明人： John C. Martin
IPC分类号： B65D85/00
CPC分类号： B65D25/205
摘要： A fuel information system, e.g., for use in conjunction with fuel storage containers, is provided. The fuel information system will include a separate or stand alone system that can be fastened to, disposed about, or affixed, to the fuel storage container that is capable of providing data to a user of the container relating to the contents and/or characteristics of the fuel contained within the fuel storage container. The data on the fuel information system can include, without limitation: (1) the type of fuel being stored (e.g., leaded, unleaded, grade (e.g., regular, mid-grade, premium); (2) the fuel/oil mixture ratio, if applicable; (3) whether or not a stabilizing agent has been added to the fuel; (4) the date the fuel was added to the container; and (5) a location or place for “where used” information indicating the type(s) of equipment the particular fuel is intended to be used in.
摘要翻译：提供燃料信息系统，例如用于燃料储存容器的燃料信息系统。燃料信息系统将包括独立或独立的系统，其可以被固定到，设置在或附着到燃料储存容器上，所述燃料储存容器能够向容器的使用者提供与内容物和/或包含在燃料储存容器内的燃料。燃料信息系统的数据可以包括但不限于：（1）储存的燃料类型（例如铅，无铅，等级（如常规，中档，高级））;（2）燃料/油混合物比例（如适用）;（3）稳定剂是否已添加到燃料中;（4）燃料添加到集装箱的日期;（5）“使用”的位置或地点，表示要使用特定燃料的设备类型。

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