专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5534428A Cloned Ssti/SacI restriction-modification system 失效
标题翻译：克隆Ssti / SacI限制修饰系统
公开(公告)号：US5534428A
公开(公告)日：1996-07-09
申请号：US300226
申请日：1994-09-06
申请人： Mary C. Longo , Michael D. Smith , Raymond D. Harris
发明人： Mary C. Longo , Michael D. Smith , Raymond D. Harris
IPC分类号： C12N9/10 , C12N9/22 , C12N15/55 , C12N15/70
CPC分类号： C12N9/22 , C12N9/1007
摘要： The present invention discloses the cloning and expression in Escherichia coli of the SstI/SacI restriction-modification system. The SstI methylase gene was cloned by methylase selection (the "Hungarian Trick" and the orientation and boundaries of the gene were established. The level of methylation of the host was improved significantly by expressing the SstI methylase from the strong RPSL promoter. Using the SstI methylase clone as a probe, a chromosomal map was generated in the area of the gene for the SstI methylase. DNA downstream from the methylase was cloned and found to contain the gene for the SstI endonuclease. The orientation of the SstI endonuclease was established and a strong promoter was placed close to the SstI endonuclease gene by generating nested deletions. By these methods, an E. coli strain was constructed which produced high levels of SstI endonuclease. The SacI locus in the S. achromogenes chromosome was found to be identical to the SstI locus in S. stanford in all measured respects.
摘要翻译：本发明公开了在SstI / SacI限制性修饰体系中克隆和表达大肠杆菌。通过甲基化酶选择（“匈牙利Trick”）克隆了SstI甲基化酶基因，建立了基因的方向和边界，通过从强RPSL启动子表达SstI甲基化酶，显着提高了宿主的甲基化水平，使用SstI 甲基化酶克隆作为探针，在SstI甲基化酶基因区域产生染色体图，克隆了甲基化酶下游的DNA，发现含有SstI内切核酸酶的基因，建立了SstI内切核酸酶的取向，通过产生嵌套缺失将强启动子置于SstI内切核酸酶基因附近，通过这些方法构建产生高水平SstI内切核酸酶的大肠杆菌菌株，发现染色体上的SacI位点与所有测量方面，斯坦福德的SstI位点。

2. 发明授权

US5759839A Cloned SstI/SacI restriction-modification system 失效
标题翻译：生产SstI / SacI限制修饰系统的方法
公开(公告)号：US5759839A
公开(公告)日：1998-06-02
申请号：US687623
申请日：1996-07-03
申请人： Mary C. Longo , Michael D. Smith , Raymond D. Harris
发明人： Mary C. Longo , Michael D. Smith , Raymond D. Harris
IPC分类号： C12N9/10 , C12N9/22 , C12N15/55 , C12N15/70
CPC分类号： C12N9/22 , C12N9/1007
摘要： The present invention discloses the cloning and expression in Escherichia coli of the SstI/SacI restriction-modification system. The SstI methylase gene was cloned by methylase selection (the "Hungarian Trick" and the orientation and boundaries of the gene were established. The level of methylation of the host was improved significantly by expressing the SstI methylase from the strong RPSL promoter. Using the SstI methylase clone as a probe, a chromosomal map was generated in the area of the gene for the SstI methylase. DNA downstream from the methylase was cloned and found to contain the gene for the SstI endonuclease. The orientation of the SstI endonuclease was established and a strong promoter was placed close to the SstI endonuclease gene by generating nested deletions. By these methods, an E. coli strain was constructed which produced high levels of SstI endonuclease. The SacI locus in the S. achromogenes chromosome was found to be identical to the SstI locus in S. stanford in all measured respects.
摘要翻译：本发明公开了在SstI / SacI限制性修饰体系中克隆和表达大肠杆菌。通过甲基化酶选择（“匈牙利Trick”）克隆了SstI甲基化酶基因，建立了基因的方向和边界，通过从强RPSL启动子表达SstI甲基化酶，显着提高了宿主的甲基化水平，使用SstI 甲基化酶克隆作为探针，在SstI甲基化酶基因区域产生染色体图，克隆了甲基化酶下游的DNA，发现含有SstI内切核酸酶的基因，建立了SstI内切核酸酶的取向，通过产生嵌套缺失将强启动子置于SstI内切核酸酶基因附近，通过这些方法构建产生高水平SstI内切核酸酶的大肠杆菌菌株，发现染色体上的SacI位点与所有测量方面，斯坦福德的SstI位点。

3. 发明授权

US5334526A Cloning and expression of AluI restriction endonuclease 失效
标题翻译： AluI限制性内切核酸酶的克隆和表达
公开(公告)号：US5334526A
公开(公告)日：1994-08-02
申请号：US68188
申请日：1993-05-28
申请人： Michael D. Smith , Brian J. Schmidt , Mary C. Longo , Deb K. Chatterjee
发明人： Michael D. Smith , Brian J. Schmidt , Mary C. Longo , Deb K. Chatterjee
IPC分类号： C12N9/22 , C12N15/63 , C12N15/70
CPC分类号： C12N9/22
摘要： The present invention is directed to recombinant hosts which contain and express the AluI Type-II restriction endonuclease gene. The present invention is also directed to vectors or DNA molecules which contain the gene, and to methods of producing the AluI enzyme. One source of the enzyme is Arthrobacter luteus, although other microorganisms may be used to isolate restriction endonuclease isoschizomers of the invention.
摘要翻译：本发明涉及含有并表达AluI II型限制性内切核酸酶基因的重组宿主。本发明还涉及含有该基因的载体或DNA分子以及产生AluI酶的方法。酶的一个来源是黄喉节杆菌，尽管其他微生物可用于分离本发明的限制性内切核酸酶异构体。

4. 发明授权

US5470740A Cloned NsiI restriction-modification system 失效
标题翻译：克隆NsiI限制修饰系统
公开(公告)号：US5470740A
公开(公告)日：1995-11-28
申请号：US145518
申请日：1993-11-04
申请人： Mary C. Longo , Michael D. Smith
发明人： Mary C. Longo , Michael D. Smith
IPC分类号： C12N9/10 , C12N9/22 , C12N15/55 , C12N15/70 , C12N1/21 , C12N5/10
CPC分类号： C12N9/22 , C12N15/70 , C12N9/1007
摘要： The present invention discloses the cloning and expression in Escherichia coli of the NsiI restriction-modification system from Neisseria sicca, utilizing a two step protocol. Initial protection of E. coli host with methylase expressed on a plasmid was required to stabilize a compatible plasmid carrying the endonuclease gene on a single DNA fragment. A chromosomal map was generated localizing the genes for NsiI methylase and endonuclease. An E. coli strain was constructed which produced high levels of NsiI endonuclease.
摘要翻译：本发明公开了利用两步法克隆和表达来自奈瑟氏球菌的NsiI限制性修饰系统的大肠杆菌。需要在质粒上表达的用甲基化酶对大肠杆菌宿主进行初始保护以使携带有单核苷酸酶切片段上的核酸内切酶基因的相容质粒稳定。产生了定位NsiI甲基化酶和内切核酸酶的基因的染色体图谱。构建产生高水平的NsiI内切核酸酶的大肠杆菌菌株。

5. 发明授权

US5312746A Cloning and expressing restriction endonucleases and modification methylases from caryophanon 失效
标题翻译：克隆并表达限制性核酸内切酶和修饰甲基化的甲基化酶
公开(公告)号：US5312746A
公开(公告)日：1994-05-17
申请号：US002032
申请日：1993-01-08
申请人： Mary C. Longo , Michael D. Smith , Deb K. Chatterjee
发明人： Mary C. Longo , Michael D. Smith , Deb K. Chatterjee
IPC分类号： C12N1/21 , C12N9/10 , C12N9/12 , C12N15/55 , C12N9/22
CPC分类号： C12N9/12 , C12N9/1007
摘要： The present invention is directed to recombinant hosts which contain and express the ClaI Type-II restriction endonuclease and/or modification methylase genes. The present invention is also directed to vectors or DNA molecules which contain these gene, and to methods of producing the enzymes. One source of these enzyme is Caryophanon latum, although other microorganisms may be used to isolate the restriction endonuclease isoschizomers and modification methylase isoschizomers of the invention.
摘要翻译：本发明涉及含有并表达ClaI II型限制性内切核酸酶和/或修饰甲基化酶基因的重组宿主。本发明还涉及含有这些基因的载体或DNA分子，以及生产酶的方法。这些酶的一个来源是Caryophanon latum，尽管其他微生物可用于分离本发明的限制性内切核酸酶异构体和修饰甲基化酶同位异构体。

6. 发明授权

US5670359A Cloned NsiI restriction-modification system 失效
标题翻译：克隆NsiI限制修饰系统
公开(公告)号：US5670359A
公开(公告)日：1997-09-23
申请号：US563266
申请日：1995-11-27
申请人： Mary C. Longo , Michael D. Smith
发明人： Mary C. Longo , Michael D. Smith
IPC分类号： C12N9/10 , C12N9/22 , C12N15/55 , C12N15/70
CPC分类号： C12N9/22 , C12N15/70 , C12N9/1007
摘要： The present invention discloses the cloning and expression in Escherichia coli of the NsiI restriction-modification system from Neisseria sicca, utilizing a two step protocol. Initial protection of the E. coli host with methylase expressed on a plasmid was required to stabilize a compatible plasmid carrying the endonuclease gene on a single DNA fragment. A chromosomal map was generated localizing the genes for NsiI methylase and endonuclease. An E. coli strain was constructed which produced high levels of NsiI endonuclease.
摘要翻译：本发明公开了利用两步法克隆和表达来自奈瑟氏球菌的NsiI限制性修饰系统的大肠杆菌。需要用质粒表达的甲基化酶对大肠杆菌宿主进行初步保护，以使在单个DNA片段上携带内切核酸酶基因的相容质粒稳定。产生了定位NsiI甲基化酶和内切核酸酶的基因的染色体图谱。构建产生高水平的NsiI内切核酸酶的大肠杆菌菌株。

7. 发明授权

US08624199B2 Sample block holder 有权
标题翻译：样品块支架
公开(公告)号：US08624199B2
公开(公告)日：2014-01-07
申请号：US13284729
申请日：2011-10-28
申请人： Matthew Barrett , Michael D. Smith , Michal Geryk , Paul Scagnetti , Richard Tovey
发明人： Matthew Barrett , Michael D. Smith , Michal Geryk , Paul Scagnetti , Richard Tovey
IPC分类号： H01J37/28 , H01J37/16
CPC分类号： H01J37/20 , G01N2223/307 , G01N2223/418 , G01N2223/616 , H01J37/16 , H01J2237/2007 , H01J2237/201 , H01J2237/28 , H01J2237/2826
摘要： A sample holder assembly includes a sample tray, a base plate, a stage mount, and a calibration standard mounted onto the stage mount. Three mating structures on the bottom of the base plate mate with corresponding structures on a stage mount that is attached to the sample stage of the SEM. An optional contacting conductor provides electrical contact between the stage mount and the base plate so that charge generated on the sample by the electron beam can leave the sample through the sample conductive layer to the sample tray, to the base plate, to the stage mount, and through the grounded stage.
摘要翻译：样品架组件包括样品托盘，基板，载物台和安装在载物台上的校准标准品。底板底部的三个配合结构与安装在扫描电镜样品台上的台架上的相应结构配合。可选的接触导体提供台架和基板之间的电接触，使得通过电子束在样品上产生的电荷可以使样品通过样品导电层到样品托盘，到基板，到载物台，并通过接地阶段。

8. 发明申请

US20120005515A1 ADAPTIVE EVENT-GUIDED SYSTEM AND METHOD FOR AVOIDING VOLTAGE EMERGENCIES 有权
标题翻译：用于避免电压紧急情况的自适应事件导向系统和方法
公开(公告)号：US20120005515A1
公开(公告)日：2012-01-05
申请号：US13201110
申请日：2010-02-11
申请人： Vijay Janapa Reddi , Meeta Sharma Gupta , Glenn Holloway , Gu-Yeon Wei , Michael D. Smith , David Brooks
发明人： Vijay Janapa Reddi , Meeta Sharma Gupta , Glenn Holloway , Gu-Yeon Wei , Michael D. Smith , David Brooks
IPC分类号： G06F11/30
CPC分类号： G06F1/28
摘要： In a preferred embodiment, the present invention is a system for avoiding voltage emergencies. The system comprises a microprocessor, an actuator for throttling the microprocessor, a voltage emergency detector and a voltage emergency predictor. The voltage emergency detector may comprise, for example, a checkpoint recovery mechanism or a sensor. The voltage emergency predictor of a preferred embodiment comprises means for tracking control flow instructions and microarchitectural events, means for storing voltage emergency signatures that cause voltage emergencies, means for comparing current control flow and microarchitectural events with stored voltage emergency signatures to predict voltage emergencies, and means for actuating said actuator to throttle said microprocessor to avoid predicted voltage emergencies.
摘要翻译：在优选实施例中，本发明是用于避免电压紧急情况的系统。该系统包括微处理器，用于节流微处理器的致动器，电压紧急检测器和电压紧急预报器。电压应急检测器可以包括例如检查点恢复机构或传感器。优选实施例的电压应急预测器包括用于跟踪控制流程指令和微架构事件的装置，用于存储引起电压紧急情况的电压紧急签名的装置，用于将电流控制流和微架构事件与存储的电压紧急签名进行比较以预测电压紧急情况的装置，以及用于致动所述致动器以节制所述微处理器以避免预测的电压紧急情况的装置。

9. 发明申请

US20100022910A1 RAPID ALLERGY TEST 审中-公开
标题翻译：快速过敏测试
公开(公告)号：US20100022910A1
公开(公告)日：2010-01-28
申请号：US12179901
申请日：2008-07-25
申请人： John A. Lane , Andrew Kugler , Corinn Fahrenkrug , Suzanne Abate Gunter , Allan Krauter , Lucas C. McCann , Ashley Elizabeth Tudor , Michael D. Smith , William Scott
发明人： John A. Lane , Andrew Kugler , Corinn Fahrenkrug , Suzanne Abate Gunter , Allan Krauter , Lucas C. McCann , Ashley Elizabeth Tudor , Michael D. Smith , William Scott
IPC分类号： A61B5/00
CPC分类号： A61B5/0059 , A61B5/411 , A61B5/441 , A61B5/6833
摘要： A flexible and disposable allergen-containing patch is provided with markings transferable to the skin. The patch may also include piercing elements to become an all-in-one system. A reusable actuator enables a user with little training or experience to administer multiple test injections with the disposable patch. A scope allows magnified viewing of a test site after the skin test is provided, the scope incorporating the patch as a releasable component. An all-in-one applicator assembly that can be bent to fit a convex engagement surface of a rolling actuator is also provided.
摘要翻译：具有灵活和一次性过敏原的贴片提供可转印至皮肤的标记。补丁还可以包括穿孔元件以成为一体式系统。可重复使用的执行器使得用户几乎没有培训或经验来管理一次性贴片的多次测试注射。范围允许在提供皮肤测试后放大观察测试点，范围包含贴片作为可释放组件。还提供了可以弯曲以配合滚动致动器的凸形接合表面的一体式施加器组件。

10. 发明授权

US07351839B2 Process for the preparation of 1,2,3,4,8,9,10, 10a-octahydro-7bH-cyclopenta[b][1,4]diazepino-[6,7,1-hi]indole derivatives 失效
标题翻译：制备1,2,3,4,8,9,10,10a-八氢-7H-环戊二烯并[b] [1,4]二氮杂 - [6,7,1-hi]吲哚衍生物的方法
公开(公告)号：US07351839B2
公开(公告)日：2008-04-01
申请号：US11602531
申请日：2006-11-21
申请人： Gregory S. Welmaker , Joan E. Sabalski , Michael D. Smith
发明人： Gregory S. Welmaker , Joan E. Sabalski , Michael D. Smith
IPC分类号： C07D209/82
CPC分类号： C07D209/94 , C07D487/06
摘要： This invention provides processes for preparing compounds formula: wherein R is H, alkyl, acyl, aryl, aroyl or —C(O)R′; R′ is alkyl or aryl; R1, R2, R4 and R5 are H, —OH, alkyl, cycloalkyl, alkoxy, halogen, fluorinated alkyl or alkoxy, —CN, —NH—SO2-alkyl, —SO2—NH-alkyl, alkyl amide, amino, alkylamino, dialkylamino, acyl, aryl or aroyl; R3 is H, alkyl, cycloalkyl, alkoxy, fluorinated alkyl, —NH—SO2-alkyl, —SO2—NH-alkyl, alkyl amide, amino, alkylamino, dialkylamino, fluorinated alkoxy, acyl, aryl, or aroyl; or a pharmaceutically acceptable salt thereof, as well as novel compounds useful in the synthesis of these compounds.
摘要翻译：本发明提供制备下式化合物的方法：其中R为H，烷基，酰基，芳基，芳酰基或-C（O）R'; R'是烷基或芳基; R 1，R 2，R 4和R 5是H，-OH，烷基，环烷基，烷氧基卤素，氟化烷基或烷氧基，-CN，-NH-SO 2 - 烷基，-SO 2 -NH-烷基，烷基酰胺，氨基，烷基氨基，二烷基氨基，酰基，芳基或芳酰基; R 3是H，烷基，环烷基，烷氧基，氟化烷基，-NH-SO 2 - 烷基，-SO 2 -NH-烷基，烷基酰胺，氨基，烷基氨基，二烷基氨基，氟化烷氧基，酰基，芳基或芳酰基; 或其药学上可接受的盐，以及用于合成这些化合物的新化合物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式