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1. 发明授权

US09753971B2 Hot deployment of embedded database procedures 有权
公开(公告)号：US09753971B2
公开(公告)日：2017-09-05
申请号：US14548574
申请日：2014-11-20
申请人： Martin Hartig , Helmut Prestel , Rolf Hammer , Kai Baumgarten , Sigrid Wortmann , Christiane Kettschau , Stefan Werner , Ralf Wendelgass
发明人： Martin Hartig , Helmut Prestel , Rolf Hammer , Kai Baumgarten , Sigrid Wortmann , Christiane Kettschau , Stefan Werner , Ralf Wendelgass
IPC分类号： G06F17/30 , G06F9/445
CPC分类号： G06F17/30386 , G06F8/30 , G06F8/47 , G06F8/656 , G06F9/44521 , G06F11/3466 , G06F17/30289 , G06F17/30297
摘要： Disclosed herein are systems and methods for the hot deployment of database procedures embedded in a host software application. In various example embodiments, the database procedures are encapsulated in specially marked methods of the host application, which, when called at runtime, cause the database procedures, and associated procedure stubs through which the database procedures are called, to be created in a database catalog and executed on the database server. When an embedded database procedure is modified in an application by one user during execution of that application by a second user, compilation of the modified database procedure may result in deletion of the original database procedure and/or associated procedure stub from the database, avoiding inconsistencies between database procedure versions during application execution by the second user.

2. 发明申请

US20160078067A1 EXTENSIBILITY OF EMBEDDED DATABASE PROCEDURES 有权
标题翻译：嵌入式数据库程序的可扩展性
公开(公告)号：US20160078067A1
公开(公告)日：2016-03-17
申请号：US14548659
申请日：2014-11-20
申请人： Martin Hartig , Helmut Prestel , Rolf Hammer , Kai Baumgarten , Sigrid Wortmann , Christiane Kettschau , Stefan Werner , Ralf Wendelgass
发明人： Martin Hartig , Helmut Prestel , Rolf Hammer , Kai Baumgarten , Sigrid Wortmann , Christiane Kettschau , Stefan Werner , Ralf Wendelgass
IPC分类号： G06F17/30
CPC分类号： G06F17/30386 , G06F8/30 , G06F8/47 , G06F8/656 , G06F9/44521 , G06F11/3466 , G06F17/30289 , G06F17/30297
摘要： Disclosed herein are systems and methods facilitating extensions to software applications that access a database, and in particular to applications with embedded database procedures. In various embodiments, an extension anchor embedded in the application as well as one or more extensions implemented as database procedures are encapsulated in specially marked database-procedure methods.
摘要翻译：这里公开了便于对访问数据库的软件应用的扩展的系统和方法，特别是涉及具有嵌入式数据库过程的应用的系统和方法。在各种实施例中，嵌入应用程序中的扩展锚以及实现为数据库过程的一个或多个扩展被封装在特别标记的数据库过程方法中。

3. 发明申请

US20160078041A1 HOT DEPLOYMENT OF EMBEDDED DATABASE PROCEDURES 有权
标题翻译：嵌入式数据库程序的热点部署
公开(公告)号：US20160078041A1
公开(公告)日：2016-03-17
申请号：US14548574
申请日：2014-11-20
申请人： Martin Hartig , Helmut Prestel , Rolf Hammer , Kai Baumgarten , Sigrid Wortmann , Christiane Kettschau , Stefan Werner , Ralf Wendelgass
发明人： Martin Hartig , Helmut Prestel , Rolf Hammer , Kai Baumgarten , Sigrid Wortmann , Christiane Kettschau , Stefan Werner , Ralf Wendelgass
IPC分类号： G06F17/30
CPC分类号： G06F17/30386 , G06F8/30 , G06F8/47 , G06F8/656 , G06F9/44521 , G06F11/3466 , G06F17/30289 , G06F17/30297
摘要： Disclosed herein are systems and methods for the hot deployment of database procedures embedded in a host software application. In various example embodiments, the database procedures are encapsulated in specially marked methods of the host application, which, when called at runtime, cause the database procedures, and associated procedure stubs through which the database procedures are called, to be created in a database catalog and executed on the database server. When an embedded database procedure is modified in an application by one user during execution of that application by a second user, compilation of the modified database procedure may result in deletion of the original database procedure and/or associated procedure stub from the database, avoiding inconsistencies between database procedure versions during application execution by the second user.
摘要翻译：这里公开了用于热嵌入主机软件应用中的数据库过程的系统和方法。在各种示例实施例中，数据库过程被封装在主机应用的特别标记的方法中，当在运行时被调用时，数据库过程引起数据库过程以及通过其调用数据库过程的关联过程存根被创建在数据库目录中并在数据库服务器上执行。当由第二用户执行该应用程序期间由一个用户在应用程序中修改嵌入式数据库过程时，修改的数据库过程的编译可能导致从数据库删除原始数据库过程和/或相关联的过程存根，从而避免不一致在由第二用户执行的应用程序期间的数据库过程版本之间。

4. 发明授权

US10061800B2 Embedding database procedures in data-driven applications 有权
公开(公告)号：US10061800B2
公开(公告)日：2018-08-28
申请号：US14548628
申请日：2014-11-20
申请人： Martin Hartig , Helmut Prestel , Rolf Hammer , Kai Baumgarten , Sigrid Wortmann , Christiane Kettschau , Stefan Werner , Ralf Wendelgass
发明人： Martin Hartig , Helmut Prestel , Rolf Hammer , Kai Baumgarten , Sigrid Wortmann , Christiane Kettschau , Stefan Werner , Ralf Wendelgass
IPC分类号： G06F17/30 , G06F9/445 , G06F8/656 , G06F8/41 , G06F8/30 , G06F11/34
CPC分类号： G06F16/24 , G06F8/30 , G06F8/47 , G06F8/656 , G06F9/44521 , G06F11/3466 , G06F16/21 , G06F16/213
摘要： Disclosed herein are systems and methods for embedding database procedures in data-driven software applications written in a language different from the database-procedure language. For example, in some embodiments, the hosting application may be written in ABAP whereas the database procedure may be written in SQLScript. In various embodiments, the database procedures are encapsulated in specially marked methods of the host application, which, when called at runtime, cause the database procedures to be created in a database catalog and executed on a database server.

5. 发明授权

US09875274B2 Extensibility of embedded database procedures 有权
公开(公告)号：US09875274B2
公开(公告)日：2018-01-23
申请号：US14548659
申请日：2014-11-20
申请人： Martin Hartig , Helmut Prestel , Rolf Hammer , Kai Baumgarten , Sigrid Wortmann , Christiane Kettschau , Stefan Werner , Ralf Wendelgass
发明人： Martin Hartig , Helmut Prestel , Rolf Hammer , Kai Baumgarten , Sigrid Wortmann , Christiane Kettschau , Stefan Werner , Ralf Wendelgass
IPC分类号： G06F9/44 , G06F9/45 , G06F17/30 , G06F9/455 , G06F9/445 , G06F11/34
CPC分类号： G06F17/30386 , G06F8/30 , G06F8/47 , G06F8/656 , G06F9/44521 , G06F11/3466 , G06F17/30289 , G06F17/30297
摘要： Disclosed herein are systems and methods facilitating extensions to software applications that access a database, and in particular to applications with embedded database procedures. In various embodiments, an extension anchor embedded in the application as well as one or more extensions implemented as database procedures are encapsulated in specially marked database-procedure methods.

6. 发明申请

US20160078042A1 EMBEDDING DATABASE PROCEDURES IN DATA-DRIVEN APPLICATIONS 审中-公开
标题翻译：数据驱动应用程序中的嵌入式数据库程序
公开(公告)号：US20160078042A1
公开(公告)日：2016-03-17
申请号：US14548628
申请日：2014-11-20
申请人： Martin Hartig , Helmut Prestel , Rolf Hammer , Kai Baumgarten , Sigrid Wortmann , Christiane Kettschau , Stefan Werner , Ralf Wendelgass
发明人： Martin Hartig , Helmut Prestel , Rolf Hammer , Kai Baumgarten , Sigrid Wortmann , Christiane Kettschau , Stefan Werner , Ralf Wendelgass
IPC分类号： G06F17/30
CPC分类号： G06F17/30386 , G06F8/30 , G06F8/47 , G06F8/656 , G06F9/44521 , G06F11/3466 , G06F17/30289 , G06F17/30297
摘要： Disclosed herein are systems and methods for embedding database procedures in data-driven software applications written in a language different from the database-procedure language. For example, in some embodiments, the hosting application may be written in ABAP whereas the database procedure may be written in SQLScript. In various embodiments, the database procedures are encapsulated in specially marked methods of the host application, which, when called at runtime, cause the database procedures to be created in a database catalog and executed on a database server.
摘要翻译：本文公开了用于将数据库过程嵌入以与数据库过程语言不同的语言编写的数据驱动的软件应用中的系统和方法。例如，在一些实施例中，托管应用程序可以用ABAP编写，而数据库过程可以用SQLScript编写。在各种实施例中，数据库过程被封装在主机应用的特别标记的方法中，当在运行时被调用时，该方法使数据库过程在数据库目录中创建并在数据库服务器上执行。

7. 发明申请

US20150184138A1 PLANT-BASED RECOMBINANT BUTYRYLCHOLINESTERASE PRODUCTION METHODS 有权
标题翻译：基于植物的重组丁基胆碱酯酶生产方法
公开(公告)号：US20150184138A1
公开(公告)日：2015-07-02
申请号：US14448545
申请日：2014-07-31
申请人： Greg Pogue , Ernie Hiatt , Romy Kandzia , Stefan Werner , Frank Thieme , Tsafrir Mor
发明人： Greg Pogue , Ernie Hiatt , Romy Kandzia , Stefan Werner , Frank Thieme , Tsafrir Mor
IPC分类号： C12N9/18 , A61K38/46
CPC分类号： C12N9/18 , A61K38/465 , C12Y301/01008
摘要： A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialyalation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.
摘要翻译：提供了一种用于生产重组丁酰胆碱酯酶（rBuChE）的新的，可靠的，易于扩展和重现的方法。通过利用植物转染方法，已经显示各种植物菌株在可接受的制造过程中产生有效和可缩放量的rBuChE，以允许可靠水平的这些酶用于所需的神经药物保护要求（包括四聚物产品）。同样，工程化植物品系中的这些方法已经显示出以四聚体形式合适地生产这些酶，其中聚糖形成和sialyalation（用于末端基团）以允许针对有机磷剂暴露的最佳效力以及在植物来源内的适当的免疫原性反应。因此，包括哺乳动物细胞内的转染和生产的总体生产方法以及从植物获得这种可靠的采购平台所涉及的工艺步骤。

8. 发明申请

US20130267021A1 SYSTEM AND METHOD OF MODULAR CLONING 审中-公开
公开(公告)号：US20130267021A1
公开(公告)日：2013-10-10
申请号：US13703123
申请日：2011-06-09
申请人： Ernst Weber , Stefan Werner , Carola Engler , Ramona Grützner , Sylvestre Marillonnet
发明人： Ernst Weber , Stefan Werner , Carola Engler , Ramona Grützner , Sylvestre Marillonnet
IPC分类号： C12N15/66
CPC分类号： C12N15/66 , C12N15/1093
摘要： System for producing a nucleic acid construct of interest, said system comprising: a set of n entry DNAs numbered 1 to n, n being an integer of at least 2, each of said n entry DNAs comprising in this order: (i) a type IIs restriction endonuclease recognition site followed by the cleavage site thereof; (ii) a sequence portion linking the cleavage site of said recognition site of item (i) with the cleavage site of the recognition site of the following item (iii), and (iii) a cleavage site of a further type Ms restriction endonuclease recognition site followed by the recognition site of said cleavage site; the cleavage sites of the type IIs restriction endonuclease recognition sites of item (iii) of entry DNAs 1 to n−1 are complementary to the cleavage sites of the type IIs restriction endonuclease recognition sites of item (i) of entry DNAs 2 to n, respectively; the cleavage site of the type Ms restriction endonuclease recognition site of item (iii) of entry DNA n is complementary to the cleavage site of the type IIs restriction endonuclease recognition site of item (i) of entry DNA 1 for allowing annealing of complementary single-stranded overhangs formed by restriction at recognition site (i) of entry DNA 1 and at recognition site (iii) of entry DNA n; said system further comprising a destination vector comprising in this order: (I) a type Ms restriction endonuclease recognition site followed by the cleavage site thereof; (II) a vector backbone preferably comprising a selectable marker gene, said vector backbone linking the cleavage sites of said recognition sites of items (I) and the following item (III); (III) a further cleavage site of a type Ms restriction endonuclease recognition site followed by the recognition site of said cleavage site, and (IV) optionally, an insert between the recognition sites of item (III) and item (i); said cleavage sites of items (I) and (III) being different and non-complementary, said recognition sites of items (I) and (III) being preferably recognitions sites of the same endonuclease.

9. 发明申请

US20130024986A1 TRANSGENIC PLANTS WITH CONTROLLED DISTRIBUTION OF A TRAIT TO PROGENY 有权
标题翻译：具有受控分配的转基因作物的转基因植物
公开(公告)号：US20130024986A1
公开(公告)日：2013-01-24
申请号：US13470812
申请日：2012-05-14
申请人： Stefan Werner , Anatoly Giritch , Serik Eliby , Sylvestre Marillonnet , Victor Klimyuk , Yuri Gleba
发明人： Stefan Werner , Anatoly Giritch , Serik Eliby , Sylvestre Marillonnet , Victor Klimyuk , Yuri Gleba
IPC分类号： A01H5/00 , C12N5/10 , A01H5/10 , A01H1/02 , A01H1/04
CPC分类号： C12N15/8274 , C12N15/8213 , C12N15/8216 , C12N15/8231 , C12N15/8265 , C12N15/8289
摘要： A process of producing transgenic multi-cellular plants or parts thereof expressing a trait of interest that has a controlled distribution of said trait to progeny, comprising (i) producing a first plant or a cell thereof having in a first locus of a nuclear chromosome a first heterologous nucleotide sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest, (ii) producing a second plant or a cell thereof having in a second locus of a nuclear chromosome homologous to said nuclear chromosome of step (i), a second heterologous nucleotide sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, and (iii) hybridising said first and said second plants or cells thereof to generate progeny exhibiting said functional trait of interest. Also disclosed is a process of producing hybrid seeds for agriculture.
摘要翻译：一种生产转基因多细胞植物或其部分表达感兴趣的性状的方法，所述转基因多细胞植物或其部分具有所述性状受控于后代的分布，其包括（i）产生在核染色体的第一位点具有的第一植物或其细胞第一异源核苷酸序列，其包含编码所述感兴趣性状的核苷酸序列的第一片段，（ii）产生具有与步骤（i）的所述核染色体同源的核染色体的第二位点的第二植物或其细胞，第二异源核苷酸序列，其包含编码所述感兴趣性状的核苷酸序列的第二片段，和（iii）使所述第一和第二植物或细胞杂交以产生表现出所述目的功能性状的后代。还公开了一种生产用于农业的杂种种子的方法。

10. 发明申请

US20110263024A1 PROCESS OF CLEAN CLONING 有权
标题翻译：清洁过程
公开(公告)号：US20110263024A1
公开(公告)日：2011-10-27
申请号：US13121019
申请日：2009-10-08
申请人： Sylvestre Marillonnet , Stefan Werner , Carola Engler , Romy Kandzia , Frank Thieme , Ernst Weber
发明人： Sylvestre Marillonnet , Stefan Werner , Carola Engler , Romy Kandzia , Frank Thieme , Ernst Weber
IPC分类号： C12N15/87
CPC分类号： C12N15/64 , C12N15/66
摘要： A process of inserting a nucleic acid sequence of interest into an acceptor nucleic acid, comprising the following steps: amplifying by PCR a DNA comprising in the following order a sequence segment U, a nucleic acid sequence segment of known nucleotide sequence K2 and a nucleic acid sequence segment of known sequence K3 using a forward primer defining a first end of the amplified DNA and a reverse primer defining a second end of the amplified DNA, said reverse primer terminating at its 3′-end in a nucleotide sequence of nucleic acid sequence segment K3; treating the linear double-stranded DNA molecules contained in the PCR product obtained in the previous step with an exonuclease to obtain a single-stranded overhang at the first end of the DNA and a single-stranded overhang comprising nucleic acid segments K2 and K3 at the second end of the DNA; and annealing the product of the previous step to a linearized double-stranded acceptor nucleic acid having at a first end thereof a single-stranded overhang complementary to the single-stranded overhang of the first end of the DNA and at a second end thereof a single-stranded overhang complementary to the single-stranded sequence segment K2 of the second end of the DNA.
摘要翻译：包括以下步骤：通过PCR扩增包含以下顺序的DNA序列的已知核苷酸序列K2的核酸序列片段和核酸的方法已知序列K3的序列片段使用限定扩增DNA第一末端的正向引物和限定扩增DNA第二末端的反向引物，所述反向引物在其核酸序列片段的核苷酸序列的3'末端终止 K3; 用外切核酸酶处理前一步骤中获得的PCR产物中包含的线性双链DNA分子，以在DNA的第一端获得单链突出端，并且在第一端包含核酸片段K2和K3的单链突出端 DNA的第二端; 并将前述步骤的产物退火到线性双链受体核酸，其在第一端具有与DNA的第一末端的单链突出部互补的单链突出端，并且在其第二端具有单链突出端与DNA的第二末端的单链序列片段K2互补的突出突出端。

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