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1. 发明申请

US20100248315A1 Fermentative vitamin C production 有权
标题翻译：发酵维生素C生产
公开(公告)号：US20100248315A1
公开(公告)日：2010-09-30
申请号：US11882456
申请日：2007-08-01
申请人： Marie-Gabrielle Beuzelin-Ollivier , Bastian Chevreux , Manuela Dalluegge , Marina Van Gelder , Markus G. Goese , Corina Hauk , Bertus P. Koekman , Connie Lee , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Dick Schipper , Masako Shinjoh , Christine Toepfer , Adrianus W.H. Vollebregt
发明人： Marie-Gabrielle Beuzelin-Ollivier , Bastian Chevreux , Manuela Dalluegge , Marina Van Gelder , Markus G. Goese , Corina Hauk , Bertus P. Koekman , Connie Lee , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Dick Schipper , Masako Shinjoh , Christine Toepfer , Adrianus W.H. Vollebregt
IPC分类号： C12P17/04 , C12N1/21
CPC分类号： C12P17/04 , C12N9/0008
摘要： The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译：本发明涉及能够直接生产L-抗坏血酸（以下也称为维生素C）的新鉴定的微生物。本发明还涉及包含参与维生素C合成的蛋白质的基因的多核苷酸序列。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸和片段编码的新多肽以及它们的功能等同物。本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或所述微生物中发酵产物的生产效率。还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

2. 发明授权

US08318462B2 Fermentative vitamin C production 有权
标题翻译：发酵维生素C生产
公开(公告)号：US08318462B2
公开(公告)日：2012-11-27
申请号：US11882456
申请日：2007-08-01
申请人： Marie-Gabrielle Beuzelin-Ollivier , Bastian Chevreux , Manuela Dalluege , Marina Van Gelder , Markus G. Goese , Corina Hauk , Bertus P. Koekman , Connie Lee , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Dick Schipper , Masako Shinjoh , Christine Toepfer , Adrianus W. H. Vollebregt
发明人： Marie-Gabrielle Beuzelin-Ollivier , Bastian Chevreux , Manuela Dalluege , Marina Van Gelder , Markus G. Goese , Corina Hauk , Bertus P. Koekman , Connie Lee , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Dick Schipper , Masako Shinjoh , Christine Toepfer , Adrianus W. H. Vollebregt
IPC分类号： C12P17/04
CPC分类号： C12P17/04 , C12N9/0008
摘要： The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译：本发明涉及能够直接生产L-抗坏血酸（以下也称为维生素C）的新鉴定的微生物。本发明还涉及包含参与维生素C合成的蛋白质的基因的多核苷酸序列。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸和片段编码的新多肽以及它们的功能等同物。本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或所述微生物中发酵产物的生产效率。还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

3. 发明申请

US20090215135A1 Novel Gene STS 22 审中-公开
标题翻译：新基因STS 22
公开(公告)号：US20090215135A1
公开(公告)日：2009-08-27
申请号：US11883542
申请日：2006-02-10
申请人： Marie-Gabrielle Beuzelin-Ollivier , Bastian Chevreux , Manuela Dalluege , Markus G. Goese , Connie Lee , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
发明人： Marie-Gabrielle Beuzelin-Ollivier , Bastian Chevreux , Manuela Dalluege , Markus G. Goese , Connie Lee , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
IPC分类号： C12P17/04 , C12N15/11 , C12N15/00 , C12N1/21 , C07K14/705 , C12N15/87
CPC分类号： C12N15/52
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译：本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或所述微生物中发酵产物的生产效率。还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

4. 发明申请

US20080160588A1 Alcohol Dehydrogenase Gene from Gluconobacter Oxydans 审中-公开
标题翻译：来自氧化葡糖杆菌的酒精脱氢酶基因
公开(公告)号：US20080160588A1
公开(公告)日：2008-07-03
申请号：US11883553
申请日：2006-02-10
申请人： Bastian Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
发明人： Bastian Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
IPC分类号： C12P17/04 , C12N15/11 , C12N15/00 , C12N1/20 , C12N9/04 , C12N15/74
CPC分类号： C12P17/04 , C12N9/0006
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译：本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或所述微生物中发酵产物的生产效率。还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

5. 发明授权

US08053218B2 Gene STS 18 有权
标题翻译：基因STS 18
公开(公告)号：US08053218B2
公开(公告)日：2011-11-08
申请号：US11883782
申请日：2006-02-10
申请人： Bastian Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
发明人： Bastian Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
IPC分类号： C12P17/04
CPC分类号： C12P17/04 , C07K14/195
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of the polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of the polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in the microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译：本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。本发明还涉及多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中多核苷酸和/或编码多肽的修饰对产量，产量和/或微生物发酵产物的生产效率。还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

6. 发明申请

US20080213852A1 Novel Gene Sts 18 有权
标题翻译：新基因Sts 18
公开(公告)号：US20080213852A1
公开(公告)日：2008-09-04
申请号：US11883782
申请日：2006-02-10
申请人： Bastian Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
发明人： Bastian Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
IPC分类号： C12P1/04 , C07H21/04 , C12N15/00 , C12N1/20 , C07K16/00 , C12N15/74
CPC分类号： C12P17/04 , C07K14/195
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译：本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或所述微生物中发酵产物的生产效率。还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

7. 发明申请

US20080193972A1 Gene Rcs 25 审中-公开
标题翻译：基因Rcs 25
公开(公告)号：US20080193972A1
公开(公告)日：2008-08-14
申请号：US11883783
申请日：2006-02-10
申请人： Bastian Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
发明人： Bastian Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
IPC分类号： C12P21/04 , C12N15/11 , C12N15/00 , C12N1/20 , C12P17/04 , C07K14/00 , C12N15/74
CPC分类号： C12P17/04 , C07K14/195
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译：本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或所述微生物中发酵产物的生产效率。还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

8. 发明申请

US20080160589A1 Gene Rcs 24 审中-公开
标题翻译：基因Rcs 24
公开(公告)号：US20080160589A1
公开(公告)日：2008-07-03
申请号：US11883843
申请日：2006-02-10
申请人： Bastien Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
发明人： Bastien Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
IPC分类号： C12P17/04 , C12N15/11 , C12N15/00 , C12N1/20 , C07K14/00 , C12N15/74
CPC分类号： C12P7/60 , C12N9/0006 , C12P17/04
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译：本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或所述微生物中发酵产物的生产效率。还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

9. 发明申请

US20080176297A1 Gene Rcs 21 审中-公开
标题翻译：基因Rcs 21
公开(公告)号：US20080176297A1
公开(公告)日：2008-07-24
申请号：US11883723
申请日：2006-02-10
申请人： Bastien Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
发明人： Bastien Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
IPC分类号： C12P17/04 , C12N15/31 , C12N15/63 , C12N1/21 , C07K14/195 , C12N15/74
CPC分类号： C12P17/04 , C07K14/195
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译：本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或所述微生物中发酵产物的生产效率。还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

10. 发明申请

US20090023191A1 Gene RCS 23 审中-公开
标题翻译：基因RCS 23
公开(公告)号：US20090023191A1
公开(公告)日：2009-01-22
申请号：US11883825
申请日：2006-02-10
申请人： Bastien Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
发明人： Bastien Chevreux , Anne F. Mayer , Anja Meury , Nigel J. Mouncey , Masako Shinjoh
IPC分类号： C12P17/04 , C07H21/00 , C12N1/21 , C12N15/11 , C12N15/31 , C12P7/60 , C12N15/74
CPC分类号： C12P17/04 , C07K14/195
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译：本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或所述微生物中发酵产物的生产效率。还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

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