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1. 发明申请

US20130183666A1 PARTIAL GENOTYPING BY DIFFERENTIAL HYBRIDIZATION 审中-公开
标题翻译：通过差异杂交进行部分基因分析
公开(公告)号：US20130183666A1
公开(公告)日：2013-07-18
申请号：US13352742
申请日：2012-01-18
申请人： Marc N. Feiglin , Joseph Mamone , Howard Gamper
发明人： Marc N. Feiglin , Joseph Mamone , Howard Gamper
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6813 , C12Q1/6827 , C12Q2525/151 , C12Q2525/186
摘要： In a method of detecting the number of repeat units in a selected short tandem repeat (STR) in a genomic sample, the genomic sample is amplified by PCR and a single stranded target DNA is selected and separated for use in differential hybridization experiments. Subsequent partial genotyping comprises the steps of admixing to the target DNA at least one fluorescent labeled STR probe oligonucleotide and one different fluorescent labeled reference probe oligonucleotide allowing hybridization to the single stranded target DNA in a hybridization experiment. Measurement of the fluorescence intensities of the probes that are bound to the repeat units of the selected STR and normalizing the fluorescence intensity of the STR probes on the base of the reference probe intensity reveals a relative fluorescence signal representing the result of the differential hybridization experiment. Also disclosed are kits for carrying out partial genotyping by differential hybridization.
摘要翻译：在基因组样品中选择的短串联重复序列（STR）中检测重复单元数目的方法中，通过PCR扩增基因组样品，并选择并分离单链靶DNA用于差异杂交实验。随后的部分基因分型包括在杂交实验中将至少一种荧光标记的STR探针寡核苷酸和一种不同荧光标记的参考探针寡核苷酸混合到靶DNA上以允许与单链靶DNA杂交的步骤。测量与所选STR的重复单元结合的探针的荧光强度并且在参考探针强度的基础上标准化STR探针的荧光强度，显示表示差异杂交实验结果的相对荧光信号。还公开了用于通过差异杂交进行部分基因分型的试剂盒。

2. 发明授权

US08470153B2 Cartridge and system for manipulating samples in liquid droplets 有权
标题翻译：用于操作液滴样品的墨盒和系统
公开(公告)号：US08470153B2
公开(公告)日：2013-06-25
申请号：US13188584
申请日：2011-07-22
申请人： Marc N. Feiglin , Joseph Mamone , Anne R. Kopf-Sill , Ian Fitzpatrick , Mark Rob , Phillip Duncan
发明人： Marc N. Feiglin , Joseph Mamone , Anne R. Kopf-Sill , Ian Fitzpatrick , Mark Rob , Phillip Duncan
IPC分类号： C02F1/04 , C02F11/00 , C25B9/00 , C25B11/00 , C25B13/00 , G01N27/00
CPC分类号： B01L3/502738 , B01L3/502715 , B01L3/502784 , B01L3/5029 , B01L3/523 , B01L3/527 , B01L7/52 , B01L2200/025 , B01L2200/027 , B01L2200/04 , B01L2200/0647 , B01L2200/141 , B01L2300/044 , B01L2300/0672 , B01L2300/0681 , B01L2300/0829 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/161 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0638 , B01L2400/0683
摘要： A cartridge (1) comprises a working film (10) for manipulating samples in liquid droplets with an electrode array (20) when the working film (10) of the cartridge (1) is placed on said electrode array (20). The cartridge (1) comprises a body (2,2′,2″) with a number of wells (5) configured to hold therein reagents (6) or samples (6′); a flexibly deformable top structure (7) impermeable to liquids and configured to seal a top side of the wells (5); a piercable bottom structure (8) impermeable to liquids and configured to seal a bottom side of the wells (5); a working film (10) located below a lower surface (4) of the body (2,2′,2″), the working film (10) being impermeable to liquids and comprising a hydrophobic upper surface (11); a peripheral spacer (9,9′,9″) located below the lower surface (4) of the body (2,2′,2″) and connecting the working film (10) to the body (2,2′,2″); a gap (12) between the lower surface (4) of the body (2,2′,2″) and the hydrophobic upper surface (11) of the working film (10), the gap (12) being defined by the peripheral spacer (9,9′,9″); and a number of piercing elements (13) located below piercable bottom structures (8) and configured to pierce the piercable bottom structures (8) for releasing reagents or samples (6,6′) from the wells (5) into the gap (12). Also disclosed is a system (40) with an electrode array (20) onto which the cartridge (1) can be placed.
摘要翻译：当药筒（1）的工作薄膜（10）被放置在所述电极阵列（20）上时，墨盒（1）包括用于用电极阵列（20）操纵液滴样品的工作薄膜（10）。墨盒（1）包括具有多个孔（5）的主体（2,2'，2“），所述孔被配置为容纳其中的试剂（6）或样品（6'）; 柔性可变形顶部结构（7），其不透液体并且构造成密封所述孔（5）的顶侧; 不透液体并且被构造成密封井（5）的底侧的可穿透底部结构（8）; 位于主体（2,2'，2“）的下表面（4）下方的工作薄膜（10），工作薄膜（10）不透液体并包括疏水性上表面（11）; 位于主体（2,2'，2“）的下表面（4）下方的外围间隔件（9,9'，9”），并将工作膜（10）连接到主体（2,2' ，2“）; 主体（2,2'，2“）的下表面（4）与工作膜（10）的疏水性上表面（11）之间的间隙（12），间隙（12）由外围间隔物（9,9'，9“）; 以及位于穿孔底部结构（8）下面的多个穿孔元件（13），并被构造成刺穿可穿透底部结构（8），用于将试剂或样品（6,6'）从孔（5）释放到间隙（12））。还公开了一种具有电极阵列（20）的系统（40），其上可以放置盒（1）。

3. 发明授权

US06449827B1 Apparatus for gripping microplates 失效
标题翻译：用于夹持微孔板的装置
公开(公告)号：US06449827B1
公开(公告)日：2002-09-17
申请号：US09686645
申请日：2000-10-10
申请人： Glenn A. Clarke , Marc N. Feiglin , Gary S. Kath , Gregory W. King
发明人： Glenn A. Clarke , Marc N. Feiglin , Gary S. Kath , Gregory W. King
IPC分类号： B23Q700
CPC分类号： G01N35/0099 , B25B11/005 , G01N35/04 , G01N2035/042 , H01L21/6838 , H01L21/68707 , Y10T29/49998 , Y10T29/53991
摘要： An apparatus suitable for securely gripping and holding a microplate or other containers during robotic de-lidding operations. A microplate placed on the apparatus is held by a vacuum drawn through the base thereof, allowing the lid of the microplate to be removed without movement of the plate. Afterwards, the vacuum can be discontinued and the plate removed from the apparatus.
摘要翻译：一种适用于在机器人脱壳操作期间可靠地夹持和保持微孔板或其他容器的装置。放置在装置上的微孔板通过其底部抽出的真空保持，允许微板的盖移除而不移动板。之后，可以停止真空并从设备中取出板。

4. 发明授权

US09108193B2 Collection/extraction container for biological material in forensic samples 有权
标题翻译：法医样品中生物材料的收集/提取容器
公开(公告)号：US09108193B2
公开(公告)日：2015-08-18
申请号：US12669922
申请日：2007-07-23
申请人： Marc N. Feiglin
发明人： Marc N. Feiglin
IPC分类号： B01L3/00 , G01N1/40
CPC分类号： B01L3/5021 , B01L3/50255 , B01L3/5029 , B01L3/50851 , B01L3/50853 , B01L3/50855 , B01L3/545 , B01L2200/027 , B01L2300/022 , B01L2300/046 , B01L2300/0618 , B01L2300/0829 , G01N1/4044 , Y10T436/25
摘要： Relates to a collection/extraction container (1) for collecting solid forensic samples (2) and/or for extracting biological material from these solid forensic samples (2) and its use. The container (1) has a top opening (3) for the insertion of a solid forensic sample (2). The container (1) comprises a basket (7) and an essentially horizontal intermediate floor (8) for the retention of the solid forensic sample (2) during digestion/lysis and extraction, the intermediate floor (8) being permeable to fluids and dividing the inner chamber (5) into an upper sample space (9) and a lower fluid space (10). The container according to the invention is characterized in that is comprises an essentially vertical channel (11), which is accomplished to allow a pipetting needle (12) to be inserted through the sample space (9) and into the fluid space (10) in that the channel (11) has a top orifice (13) that is situated nearby a top opening (3) of the container (1) and in that the channel (11) penetrates the intermediate floor (8) in a bottom orifice (14), wherein the container (1) also comprises a partition wall (15) that at least partially surrounds the channel (11) and that separates the latter from the upper sample space (9).
摘要翻译：涉及收集/提取容器（1），用于收集固体法医样品（2）和/或从这些固体法医样品（2）中提取生物材料及其用途。容器（1）具有用于插入固体法医样品（2）的顶部开口（3）。容器（1）包括篮子（7）和基本上水平的中间地板（8），用于在消解/裂解和提取期间保持固体法医样品（2），中间地板（8）可渗透流体和分割所述内室（5）进入上部样品空间（9）和下部流体空间（10）。根据本发明的容器的特征在于，其包括基本上垂直的通道（11），其实现为允许移液针（12）通过样品空间（9）插入到流体空间（10）中通道（11）具有位于容器（1）的顶部开口（3）附近的顶部孔口（13），并且通道（11）穿过底部孔口（14）中的中间地板（8）），其中所述容器（1）还包括至少部分地围绕所述通道（11）并将其与所述上部样品空间（9）分离的分隔壁（15）。

5. 发明申请

US20100248213A1 COLLECTION/EXTRACTION CONTAINER FOR BIOLOGICAL MATERIAL IN FORENSIC SAMPLES 有权
标题翻译：生物材料的收集/抽取容器在威尔逊样品中的应用
公开(公告)号：US20100248213A1
公开(公告)日：2010-09-30
申请号：US12669922
申请日：2007-07-23
申请人： Marc N. Feiglin
发明人： Marc N. Feiglin
IPC分类号： G01N1/28 , C12Q1/68 , C12Q1/70 , C12Q1/02 , G01N33/68 , B01L3/00
CPC分类号： B01L3/5021 , B01L3/50255 , B01L3/5029 , B01L3/50851 , B01L3/50853 , B01L3/50855 , B01L3/545 , B01L2200/027 , B01L2300/022 , B01L2300/046 , B01L2300/0618 , B01L2300/0829 , G01N1/4044 , Y10T436/25
摘要： Relates to a collection/extraction container (1) for collecting solid forensic samples (2) and/or for extracting biological material from these solid forensic samples (2) and its use. The container (1) has a top opening (3) for the insertion of a solid forensic sample (2). The container (1) comprises a basket (7) and an essentially horizontal intermediate floor (8) for the retention of the solid forensic sample (2) during digestion/lysis and extraction, the intermediate floor (8) being permeable to fluids and dividing the inner chamber (5) into an upper sample space (9) and a lower fluid space (10). The container according to the invention is characterized in that is comprises an essentially vertical channel (11), which is accomplished to allow a pipetting needle (12) to be inserted through the sample space (9) and into the fluid space (10) in that the channel (11) has a top orifice (13) that is situated nearby a top opening (3) of the container (1) and in that the channel (11) penetrates the intermediate floor (8) in a bottom orifice (14), wherein the container (1) also comprises a partition wall (15) that at least partially surrounds the channel (11) and that separates the latter from the upper sample space (9).
摘要翻译：涉及收集/提取容器（1），用于收集固体法医样品（2）和/或从这些固体法医样品（2）中提取生物材料及其用途。容器（1）具有用于插入固体法医样品（2）的顶部开口（3）。容器（1）包括篮子（7）和基本上水平的中间地板（8），用于在消解/裂解和提取期间保持固体法医样品（2），中间地板（8）可渗透流体和分割所述内室（5）进入上部样品空间（9）和下部流体空间（10）。根据本发明的容器的特征在于，其包括基本上垂直的通道（11），其实现为允许移液针（12）通过样品空间（9）插入到流体空间（10）中通道（11）具有位于容器（1）的顶部开口（3）附近的顶部孔口（13），并且通道（11）穿过底部孔口（14）中的中间地板（8）），其中所述容器（1）还包括至少部分地围绕所述通道（11）并将其与所述上部样品空间（9）分离的分隔壁（15）。

6. 发明授权

US08882982B2 System and instrument for processing biological samples and manipulating liquids having biological samples 有权
标题翻译：用于处理生物样品和操作具有生物样品的液体的系统和仪器
公开(公告)号：US08882982B2
公开(公告)日：2014-11-11
申请号：US13139647
申请日：2009-12-16
申请人： Marc N. Feiglin
发明人： Marc N. Feiglin
IPC分类号： G01N27/453 , B01L3/00 , B01L3/02
CPC分类号： B01L3/502792 , B01F13/0071 , B01F13/0076 , B01F2215/0037 , B01L3/0268 , B01L3/502715 , B01L3/527 , B01L7/525 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2200/16 , B01L2300/0636 , B01L2300/0819 , B01L2300/1822 , B01L2300/1883 , B01L2400/0409 , B01L2400/0415 , B01L2400/0427 , B01L2400/0475 , C12Q1/686 , G01N27/447 , G01N27/44756 , G01N27/453 , C12Q2563/143 , C12Q2563/149 , C12Q2563/159
摘要： A biological sample processing system comprises a container for large volume processing, a flat polymer film having a lower surface and a hydrophobic upper surface, which is kept at a distance d to the base side of the container by the protrusions. The distance d defines at least one gap when the container is positioned on the film. A liquid droplet manipulation instrument comprises at least one electrode array for inducing liquid droplet movements; a substrate supporting the at least one electrode array; and a control unit is characterized in that the container and the film are reversibly attached to the liquid droplet manipulation instrument. The system thus enables displacement of at least one liquid droplet from the at least one well through the channel of the container onto the hydrophobic upper surface of the flat polymer film and above the at least one electrode array; wherein the liquid droplet manipulation instrument controls a guided movement of the liquid droplet on the hydrophobic upper surface of the flat polymer film by electrowetting and to process there the biological sample.
摘要翻译：生物样品处理系统包括用于大体积加工的容器，具有下表面和疏水性上表面的平坦聚合物膜，其通过突起保持在容器底部的距离d处。当容器位于胶片上时，距离d限定至少一个间隙。液滴操作仪器包括用于引起液滴运动的至少一个电极阵列; 支撑所述至少一个电极阵列的基板; 并且控制单元的特征在于，容器和膜可逆地附接到液滴操纵仪器。因此，该系统使得至少一个液滴从至少一个井通过容器的通道移位到平坦聚合物膜的疏水性上表面上并位于至少一个电极阵列上方; 其特征在于，所述液滴操作装置通过电润湿来控制所述液滴在所述平坦聚合物膜的所述疏水性上表面上的引导移动，并在所述生物样品处理。

7. 发明申请

US20110290647A1 SYSTEM AND INSTRUMENT FOR PROCESSING BIOLOGICAL SAMPLES AND MANIPULATING LIQUIDS HAVING BIOLOGICAL SAMPLES 有权
标题翻译：用于处理生物样品和具有生物样品的操作液体的系统和仪器
公开(公告)号：US20110290647A1
公开(公告)日：2011-12-01
申请号：US13139647
申请日：2009-12-16
申请人： Marc N. Feiglin
发明人： Marc N. Feiglin
IPC分类号： G01N27/447 , C12Q1/68 , G01N27/453
CPC分类号： B01L3/502792 , B01F13/0071 , B01F13/0076 , B01F2215/0037 , B01L3/0268 , B01L3/502715 , B01L3/527 , B01L7/525 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2200/16 , B01L2300/0636 , B01L2300/0819 , B01L2300/1822 , B01L2300/1883 , B01L2400/0409 , B01L2400/0415 , B01L2400/0427 , B01L2400/0475 , C12Q1/686 , G01N27/447 , G01N27/44756 , G01N27/453 , C12Q2563/143 , C12Q2563/149 , C12Q2563/159
摘要： A biological sample processing system comprises a container for large volume processing, a flat polymer film having a lower surface and a hydrophobic upper surface, which is kept at a distance d to the base side of the container by the protrusions. The distance d defines at least one gap when the container is positioned on the film. A liquid droplet manipulation instrument comprises at least one electrode array for inducing liquid droplet movements; a substrate supporting the at least one electrode array; and a control unit is characterized in that the container and the film are reversibly attached to the liquid droplet manipulation instrument. The system thus enables displacement of at least one liquid droplet from the at least one well through the channel of the container onto the hydrophobic upper surface of the flat polymer film and above the at least one electrode array; wherein the liquid droplet manipulation instrument controls a guided movement of the liquid droplet on the hydrophobic upper surface of the flat polymer film by electrowetting and to process there the biological sample.
摘要翻译：生物样品处理系统包括用于大体积加工的容器，具有下表面和疏水性上表面的平坦聚合物膜，其通过突起保持在容器底部的距离d处。当容器位于胶片上时，距离d限定至少一个间隙。液滴操作仪器包括用于引起液滴运动的至少一个电极阵列; 支撑所述至少一个电极阵列的基板; 并且控制单元的特征在于，容器和膜可逆地附接到液滴操纵仪器。因此，该系统使得至少一个液滴从至少一个井通过容器的通道移位到平坦聚合物膜的疏水性上表面上并位于至少一个电极阵列上方; 其特征在于，所述液滴操作装置通过电润湿来控制所述液滴在所述平坦聚合物膜的所述疏水性上表面上的引导移动，并在所述生物样品处理。

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