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1. 发明授权

US5173416A L-carnitine dehydrogenase from alcalgenes 失效
标题翻译：来自ALCALGENES的L-CARNITINE DEHYDROGENASE
公开(公告)号：US5173416A
公开(公告)日：1992-12-22
申请号：US596994
申请日：1990-10-12
申请人： Mamoru Takahashi , Kazuo Matsuura , Shinobu Nagasawa
发明人： Mamoru Takahashi , Kazuo Matsuura , Shinobu Nagasawa
IPC分类号： C12N1/20 , C12N9/04 , C12R1/05
CPC分类号： C12N9/0006 , Y10S435/822 , Y10S435/829
摘要： A new class of L-carnitine dehydrogenase is disclosed, which is stable in solution and has a residual activity of greater than 70% after one week in a pH 9.0 buffer solution at 5.degree. C., a molecular weight of 51,000.+-.6,000 daltons, a pH optimum of 9.0, a optimum temperature of 50.degree. C. and an isoelectric point of pH 5.3.+-.0.6. Also disclosed is a process for producing the new enzyme from a microorganism of the Alcaligenes genus, preferably the newly-discovered species Alcaligenes sp. No. 981 FERM BP-2570.

2. 发明授权

US5156966A L-carnitine dehydrogenase and process for its production 失效
标题翻译： L-CARNITINE脱氢酶及其生产方法
公开(公告)号：US5156966A
公开(公告)日：1992-10-20
申请号：US726200
申请日：1991-07-05
申请人： Mamoru Takahashi , Kazuo Matsuura , Shinobu Nagasawa
发明人： Mamoru Takahashi , Kazuo Matsuura , Shinobu Nagasawa
IPC分类号： C12N1/20 , C12N9/04 , C12R1/05
CPC分类号： C12N9/0006 , Y10S435/822 , Y10S435/829
摘要： An isolated L-carnitine dehydrogenase is disclosed, which is stable in solution and has a residual activity of greater than 70% after one week in a pH 9.0 buffer solution at 5.degree. C. Also disclosed is a process for producing the new enzyme from a microorganism of the Alcaligenes genus, preferably the newly-discovered species Alcaligenes sp. No. 981 FERM BP-2570.
摘要翻译：公开了一种分离的L-肉碱脱氢酶，其在溶液中是稳定的，并且在5℃的pH 9.0缓冲溶液中在一周后具有大于70％的残留活性。还公开了一种从产碱杆菌属的微生物，优选新发现的产碱杆菌属 981 FERM BP-2570。

3. 发明授权

US5116748A Process for the production of L-alanine dehydrogenase from 78-3 FERM BP-2517 失效
标题翻译：从78-3 FERM BP-2517生产L-苯胺脱氢酶的方法
公开(公告)号：US5116748A
公开(公告)日：1992-05-26
申请号：US413431
申请日：1989-09-26
申请人： Mamoru Takahashi , Shinobu Nagasawa , Shigeru Ikuta , Kazuo Matsuura
发明人： Mamoru Takahashi , Shinobu Nagasawa , Shigeru Ikuta , Kazuo Matsuura
IPC分类号： C12N1/20 , C12N9/06 , C12R1/01
CPC分类号： C12N9/0016 , C12R1/01
摘要： A process for the production of L-alanine dehydrogenase which comprises culturing an L-alanine dehydrogenase producing microorganism belonging to the genus Sporolactobacillus in a nutrient medium and isolating thus-produced L-alanine dehydrogenase from the cultured mass. The microorganism is for example Sporolactobacillus sp. 78-3 FERM BP-2517 and mutants and variants thereof having the ability to produce L-alanine dehydrogenase in recoverable amounts. This strain is a thermophile which cannot grow at 40.degree. C. but can grow at 45.degree. C. and 52.degree. C. The strain can assimilate glucose and produce lactic acid and acetic acid.

4. 发明授权

US5126246A Reagent for analysis of triglycerides and analysis using the same 失效
标题翻译：用于分析甘油三酸酯的试剂和使用其分析
公开(公告)号：US5126246A
公开(公告)日：1992-06-30
申请号：US435003
申请日：1989-11-09
申请人： Shigeyuki Imamura , Mamoru Takahashi , Hideo Misaki , Kazuo Matsuura
发明人： Shigeyuki Imamura , Mamoru Takahashi , Hideo Misaki , Kazuo Matsuura
IPC分类号： G01N33/92 , C12Q1/44 , C12Q1/48 , C12Q1/61
CPC分类号： C12Q1/48 , C12Q1/44 , Y10S435/81 , Y10S435/832 , Y10S436/815 , Y10S530/825 , Y10S530/83
摘要： Reagent for analysis of triglycerides contained in blood serum is provided, which comprises lipases and monoglyceride lipases capable of acting on monoglycerides having substrate specificity and capable of catalyzing the following enzymatic reaction: monoglyceride+H.sub.2 O.fwdarw.glycerol+fatty acids. The glycerol or fatty acids are measured to learn an amount of the triglycerides or fatty acid by any known analytical method.
摘要翻译：提供了用于分析血清中含有的甘油三酯的试剂，其包含能够作用于具有底物特异性并能够催化以下酶促反应的单酸甘油酯的脂肪酶和甘油单酯脂肪酶：单酸甘油酯+ H 2 O->甘油+脂肪酸。测量甘油或脂肪酸，以通过任何已知的分析方法学习一定量的甘油三酯或脂肪酸。

5. 发明授权

US4921786A Novel NAD synthetase, assay method using said novel NAD synthetase and a process for production thereof 失效
标题翻译：新型NAD合成酶，使用所述新型NAD合成酶的测定方法及其生产方法
公开(公告)号：US4921786A
公开(公告)日：1990-05-01
申请号：US94813
申请日：1987-09-10
申请人： Mamoru Takahashi , Hideo Misaki , Shigeyuki Imamura , Kazuo Matsuura
发明人： Mamoru Takahashi , Hideo Misaki , Shigeyuki Imamura , Kazuo Matsuura
IPC分类号： C12N9/00 , C12P1/00 , C12P3/00
CPC分类号： C12N9/93 , C12P1/00 , C12P3/00 , C12R1/07
摘要： A novel NAD synthetase is produced by culturing a broth of Bacillus stearothermophilus H-804 FERM BP-1408. This new enzyme selectively catalyzes the reaction ##STR1## without catalyzing the reaction ##STR2## The enzyme uses ammonia or ammonium ion as a substrate, but does not use either glutamine or asparagine. Also disclosed is an assay method using the enzyme, for any one of ATP, deamide-NAD, ammonia or ammonium ion in a specimen to be assayed.

6. 发明授权

US5079158A Novel monoglyceride lipase and its production process. 失效
标题翻译：新型单糖脂及其生产工艺。
公开(公告)号：US5079158A
公开(公告)日：1992-01-07
申请号：US171272
申请日：1988-03-21
申请人： Shigeyuki Imamura , Mamoru Takahashi , Hideo Misaki , Kazuo Matsuura
发明人： Shigeyuki Imamura , Mamoru Takahashi , Hideo Misaki , Kazuo Matsuura
IPC分类号： C12N9/18 , C12Q1/44 , C12R1/07
CPC分类号： C12N9/18 , C12Q1/44 , Y10S435/832
摘要： Disclosed herein is a novel monoglyceride lipase at least capable of catalyzing an enzymatic reaction of the following equation (a) and as substrate specificity, capable of acting on monoglyceride but incapable of acting on diglyceride and triglyceride:(a) Monoglyceride+H.sub.2 O.fwdarw.Glycerol+Fatty acidThe monoglyceride lipase is produced by culturing a specific monoglyceride lipase producing microorganism of Bacillus and then collecting the monoglyceride lipase from the resulting culture. A method is also disclosed for the analysis of a monoglyceride-containing sample solution. The monoglyceride lipase is caused to act on the sample solution upon measurement of the monoglyceride in the sample solution. Either one of glycerol and the fatty acid formed in accordance with the equation (a) is then measured.

7. 发明授权

US5356790A Highly sensitive assay method for myo-inositol, composition for practicing same, novel myo-inositol dehydrogenase, and process for producing same 失效
标题翻译：肌醇的高灵敏度测定方法，实践相同的组合物，新型肌醇脱氢酶及其制备方法
公开(公告)号：US5356790A
公开(公告)日：1994-10-18
申请号：US106693
申请日：1993-08-16
申请人： Shigeru Ueda , Mamoru Takahashi , Hideo Misaki , Shigeyuki Imamura , Kazuo Matsuura
发明人： Shigeru Ueda , Mamoru Takahashi , Hideo Misaki , Shigeyuki Imamura , Kazuo Matsuura
IPC分类号： C12N9/04 , C12Q1/02 , C12Q1/32 , C12Q1/26 , C07H1/00 , C12N11/00
CPC分类号： C12N9/0006 , C12Q1/02 , C12Q1/32 , C12R1/07 , Y10S436/817
摘要： Myo-inositol in a specimen is assayed by reacting a specimen containing myo-inositol with:a) myo-inositol dehydrogenase using a thio-NADP group or thio-NAD group and an NADP group or NAD group as coenzymes, and which catalyzes a reversible reaction forming myo-inosose from myo-inositol,b ) A.sub.1 andc) B.sub.1to effect a cycling reaction ##STR1## wherein A.sub.1 is a thio-NADP group, thio-NAD group, NADP group or NAD group, A.sub.2 is a reduced form of A.sub.1, when A.sub.1 is a thio-NADP group or thio-NAD group, B.sub.1 is a reduced NADP group or reduced NAD group and when A.sub.1 is an NADP group or NAD group, B.sub.1 is a reduced thio-NADP group or reduced thio-NAD group, and wherein B.sub.2 is an oxidized form of B.sub.1. The change in the amount of A.sub.2 generated or B.sub.1 consumed by the cycling reaction is measured to perform the assay. A composition for performing the assay comprises the above myo-inositol dehydrogenase, as well as the above components A.sub.1 and B.sub.1. The myo-inositol dehydrogenase can be produced by culturing a suitable microorganism belonging to genus Bacillus, particularly Bacillus sp. No. 3 FERM BP-3013.
摘要翻译：通过使含有肌醇的样品与以下物质反应来测定样品中的肌醇：a）使用硫代NADP基团或硫代NAD基团和NADP基团或NAD基团作为辅酶的肌醇脱氢酶，并催化可逆的由肌球蛋白形成肌内单糖的反应，b）A1和c）B1进行循环反应，其中A1是硫代NADP基团，硫代NAD基团，NADP基团或NAD基团，A2是还原形式的A1，当A1是硫代NADP基团或硫代NAD基团时，B1是还原的NADP基团或还原的NAD基团，当A1是NADP基团或NAD基团时，B1是还原的硫代NADP基团或还原的硫代 - NAD基，其中B2是B1的氧化形式。测量由循环反应消耗的A2产生量或B1的量的变化以进行测定。用于进行测定的组合物包含上述肌醇脱氢酶，以及上述组分A1和B1。肌醇脱氢酶可以通过培养属于芽孢杆菌属（Bacillus），特别是芽孢杆菌属（Bacillus sp。）的合适的微生物来产生。 3号FERM BP-3013。

8. 发明授权

US08993283B2 Production method for biomass alcohol 有权
标题翻译：生物质酒精生产方法
公开(公告)号：US08993283B2
公开(公告)日：2015-03-31
申请号：US12935499
申请日：2009-03-31
申请人： Kazuo Matsuura , Fusatsugu Abe , Tetsuo Fukazu , Takuji Cho , Kousuke Kimoto
发明人： Kazuo Matsuura , Fusatsugu Abe , Tetsuo Fukazu , Takuji Cho , Kousuke Kimoto
IPC分类号： C12P7/02 , C12P7/06 , C12P7/10 , C07B63/00 , B05B17/06
CPC分类号： C12P7/10 , B05B17/0615 , Y02E50/16 , Y02E50/17
摘要： A production method for biomass-alcohol includes a saccharification step of saccharifying biomass, a first concentrating step of ultrasonically vibrating the saccharified solution and atomizing the saccharified solution into a mist, so as to elevate the sugar concentration in the saccharified solution by removing water from the saccharified solution, a fermentation step of fermenting the saccharified solution concentrated in the first concentrating step to form an alcohol water solution, and second concentrating step of separating alcohol from the alcohol water solution fermented in the fermentation step.
摘要翻译：生物质醇的制造方法包括糖化生物质的糖化步骤，超声振动糖化溶液并将糖化溶液雾化成雾的第一浓缩步骤，以通过从糖化溶液中除去水来提高糖化溶液中的糖浓度糖化溶液，在第一浓缩步骤中发酵浓缩的糖化溶液以形成醇水溶液的发酵步骤，以及在发酵步骤中发酵的醇水溶液中分离醇的第二浓缩步骤。

9. 发明申请

US20110186490A1 APPARATUS FOR CONCENTRATING A SOLUTION 有权
标题翻译：集中解决方案的设备
公开(公告)号：US20110186490A1
公开(公告)日：2011-08-04
申请号：US12997074
申请日：2009-06-11
申请人： Kazuo Matsuura , Ichiko Azuma , Fusatsugu Abe
发明人： Kazuo Matsuura , Ichiko Azuma , Fusatsugu Abe
IPC分类号： B01D17/00
CPC分类号： B01D1/18 , B01D1/16 , B01D1/20
摘要： An apparatus for concentrating solution includes a plurality of nozzles (1) to spray solution into minute particle mist, a gas supplier (2) to transfer gas-mist mixture containing mist by supplying carrier gas to mist sprayed from the nozzle (1), and a separator (3) to separate a component with a low boiling point from a component with a high boiling point by supplying gas-mist mixture transferred using the gas supplier (2). The gas supplier (2) defines a plurality of apertures (9) to supply carrier gas to the plurality of nozzles (1). In this apparatus for concentrating solution, the plurality of nozzles (1) are spraying mist into carrier gas supplied from the apertures (9).
摘要翻译：用于浓缩溶液的装置包括：多个喷嘴（1），用于将溶液喷射到微小颗粒雾中;气体供应器（2），用于通过向喷嘴（1）喷射的雾状物供给载气来转移含有雾的气雾混合物;以及通过提供使用气体供给器（2）传送的气雾混合物，将具有低沸点的组分与高沸点组分分离的分离器（3）。气体供应器（2）限定多个孔（9）以将载气供应到多个喷嘴（1）。在这种集中溶液装置中，多个喷嘴（1）将喷雾喷雾到从孔（9）供应的载气中。

10. 发明授权

US07946508B2 Method and apparatus for separating a solution 有权
标题翻译：用于分离溶液的方法和装置
公开(公告)号：US07946508B2
公开(公告)日：2011-05-24
申请号：US12071355
申请日：2008-02-20
申请人： Kazuo Matsuura , Tetsuo Fukazu , Taisuke Sekimoto
发明人： Kazuo Matsuura , Tetsuo Fukazu , Taisuke Sekimoto
IPC分类号： A24F25/00
CPC分类号： B01D1/0052 , B01D3/06 , B01D3/145 , B01D3/346 , B01D5/0027 , B01D5/0039 , B01D5/0042 , B01D5/0045 , B01D5/0081 , B01D5/0087 , B01D5/009 , C12G3/10
摘要： A method and apparatus for separating a solution containing a target substance. The solution is atomized into a mist in an atomizer (1) to produce a mixed fluid of mist and gas. In the collection of the mist from this mixed fluid, a gas transmission membrane (51) is used. The gas transmission membrane has a pore size that transmits gas but does not transmit the target substance contained in the mist. The mixed fluid is brought into contact with the primary surface of the gas transmission membrane (51), and the pressure on the primary surface is made higher than the pressure on the secondary surface of the opposite side. Thus, the gas in the mixed fluid is allowed to pass through the gas transmission membrane (51) to separate part or all of the gas contained in the mixed fluid.
摘要翻译：一种用于分离含有目标物质的溶液的方法和装置。将溶液雾化成雾化器（1）中的雾以产生雾气和气体的混合流体。在从该混合流体收集雾中，使用气体透过膜（51）。气体透过膜具有透过气体但不透过雾中含有的目标物质的孔径。使混合流体与气体传递膜（51）的主表面接触，并使主表面上的压力高于相对侧的二次表面上的压力。因此，允许混合流体中的气体通过气体传递膜（51）以分离混合流体中包含的部分或全部气体。

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