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1. 发明授权

US5571514A Fibronectin binding protein as well as its preparation 失效
标题翻译：纤连蛋白结合蛋白及其制备
公开(公告)号：US5571514A
公开(公告)日：1996-11-05
申请号：US259000
申请日：1994-06-13
申请人： Magnus Hook , Martin K. Lindberg , Lars C. Signas , Torkel M. Wadstrom , Gunnar Froman
发明人： Magnus Hook , Martin K. Lindberg , Lars C. Signas , Torkel M. Wadstrom , Gunnar Froman
IPC分类号： C07K14/31 , A61K39/085
CPC分类号： C07K14/31
摘要： The present invention relates to a new recombinant hybrid-DNA-molecule comprising nucleotide sequence from S. aureus coding for a protein, or polypeptide, having fibronectin binding properties.
摘要翻译：本发明涉及包含编码具有纤连蛋白结合特性的蛋白质或多肽的来自金黄色葡萄球菌的核苷酸序列的新的重组杂交DNA分子。

2. 发明授权

US5320951A Fibronectin binding protein as well as its preparation 失效
标题翻译：纤连蛋白结合蛋白及其制备
公开(公告)号：US5320951A
公开(公告)日：1994-06-14
申请号：US7817
申请日：1993-01-22
申请人： Magnus Hook , Martin K. Lindberg , Lars C. Signas , Torkel M. Wadstrom , Gunnar Froman
发明人： Magnus Hook , Martin K. Lindberg , Lars C. Signas , Torkel M. Wadstrom , Gunnar Froman
IPC分类号： C07K14/31 , C12N1/21 , C12N15/31 , C07K7/00 , C07K13/00
CPC分类号： C07K14/31
摘要： The present invention relates to a new recombinant hybrid-DNA-molecule comprising a nucleotide sequence from S. aureus coding for a protein, or polypeptide, having fibronectin binding properties.
摘要翻译：本发明涉及包含编码具有纤连蛋白结合特性的蛋白质或多肽的来自金黄色葡萄球菌的核苷酸序列的新的重组杂交DNA分子。

3. 发明授权

US5770702A Fibronectin binding protein as well as its preparation 失效
公开(公告)号：US5770702A
公开(公告)日：1998-06-23
申请号：US729767
申请日：1996-10-07
申请人： Magnus Hook , Martin Kjell Lindberg , Lars Christer Signas , Torkel Mikael Wadstrom , Gunnar Froman
发明人： Magnus Hook , Martin Kjell Lindberg , Lars Christer Signas , Torkel Mikael Wadstrom , Gunnar Froman
IPC分类号： C07K14/31 , C07K13/00 , C12P21/00
CPC分类号： C07K14/31
摘要： The present invention relates to a new recombinant hybrid-DNA-molecule comprising a nucleotide sequence from S. aureus coding for a protein, or polypeptide, having fibronectin binding properties.

4. 发明申请

US20100120596A1 SEPARATION DEVICE 审中-公开
标题翻译：分离装置
公开(公告)号：US20100120596A1
公开(公告)日：2010-05-13
申请号：US12597925
申请日：2008-05-08
申请人： Gunnar Froman , Lars Haneskog , Bjorn A. Johansson , Henrik Ostlin
发明人： Gunnar Froman , Lars Haneskog , Bjorn A. Johansson , Henrik Ostlin
IPC分类号： B04B13/00
CPC分类号： B01L3/5021 , B01L3/50215 , B01L2200/026 , B01L2300/087 , B01L2400/0633
摘要： A separation device adapted for separation of a wanted end product from a sample by centrifugation. According to the invention the device comprises: a first, a second and a third compartment; a first fluid connection between the first and second compartments, arranged to be opened and closed by first closing means; a second fluid connection between the second and the third compartments, arranged to be opened and closed by second closing means. The separation device is adapted to be subjected to centrifugation after having been filled with a first density gradient medium in the first compartment, sample in the third compartment and a second density gradient medium or sample in the second compartment, whereby after centrifugation the wanted end product will settle in the second compartment and the first fluid connection and the second fluid connection will be closed by the first closing means and the second closing means respectively.
摘要翻译：一种分离装置，其适用于通过离心分离所需的最终产物与样品。根据本发明，该装置包括：第一，第二和第三隔室; 第一和第二隔室之间的第一流体连接，被布置成由第一关闭装置打开和关闭; 第二和第三隔室之间的第二流体连接，被布置成由第二关闭装置打开和关闭。分离装置适于在填充第一隔室中的第一密度梯度介质，第三隔室中的样品和第二隔室中的第二密度梯度介质或样品之后进行离心，由此在将所需最终产物将沉积在第二隔室中，并且第一流体连接和第二流体连接将分别由第一关闭装置和第二关闭装置关闭。

5. 发明申请

US20110124106A1 SEPARATION DEVICE 审中-公开
标题翻译：分离装置
公开(公告)号：US20110124106A1
公开(公告)日：2011-05-26
申请号：US13055800
申请日：2009-07-20
申请人： Gunnar Froman , Henrik Ostlin
发明人： Gunnar Froman , Henrik Ostlin
IPC分类号： C12N5/071 , B01D35/28
CPC分类号： B01L3/5021 , B01L3/0217 , B01L2400/0478 , G01N33/491
摘要： A separation device for separating a wanted end product from a liquid sample comprises a container (2) having a first end (5) and a second end (7), the first end having a central orifice (6), a plunger (3) slideably disposed in the container (2) to define a variable liquid receiving chamber between the plunger (3) and the orifice (6), and a permeable partition member (9) mounted to the plunger (3) in a spaced relationship thereto to define a compartment (12) between the partition member (9) and the plunger (3) for receiving liquid density gradient medium (13), wherein liquid may be drawn into the container (2) and expelled therefrom, respectively, through the orifice (6) by movement of the plunger (3) relative to the container (2). A method for separating a wanted end product from a liquid sample is also disclosed.
摘要翻译：用于将所需最终产品与液体样品分离的分离装置包括具有第一端（5）和第二端（7）的容器（2），所述第一端具有中心孔（6），柱塞（3）可滑动地设置在容器（2）中以在柱塞（3）和孔口（6）之间限定可变的液体接收室，以及以与其间隔开的关系安装到柱塞（3）上的可渗透分隔构件（9），以限定分隔构件（9）和用于接收液体密度梯度介质（13）的柱塞（3）之间的隔室（12），其中液体可以被吸入容器（2）并分别通过孔口6排出）通过柱塞（3）相对于容器（2）的移动。还公开了从液体样品中分离所需最终产物的方法。

6. 发明申请

US20120156779A1 METHOD FOR CELL EXPANSION 审中-公开
标题翻译：细胞扩增方法
公开(公告)号：US20120156779A1
公开(公告)日：2012-06-21
申请号：US13392538
申请日：2010-08-23
申请人： Cecilia Anneren , Andreas Axen , Asa Bjurling , Christine Sund Lundstrom , James Van Alstine , Gunnar Froman
发明人： Cecilia Anneren , Andreas Axen , Asa Bjurling , Christine Sund Lundstrom , James Van Alstine , Gunnar Froman
IPC分类号： C12N5/02
CPC分类号： C12N5/0068 , C12N5/0018 , C12N2533/70
摘要： The present invention relates to a method for cell expansion. In the method, preferably a cell culture product is used, such as a microcarrier, or other adherent cell culture surface, comprising degradable polysaccharide, preferably starch, modified with small molecular weight cell-binding ligands. This allows recovery (detachment) of adhered cells to be aided by degradation of the culture surface with enzymatic agents, such as amylase. The method for cell expansion comprises the following steps: a) adding cells, culture medium and cell culture surface comprising a degradable polysaccharide with guanidine group containing ligands to a bioreactor; b) expanding said cells by adherent cell culture; and c) aiding the detachment of said cells by exposing them to a polysaccharidase to degrade the culturing surface.
摘要翻译：本发明涉及一种细胞扩增方法。在该方法中，优选使用细胞培养产物，例如微载体或其它贴壁细胞培养物表面，其包含用小分子量细胞结合配体修饰的可降解多糖，优选淀粉。这允许通过用酶剂如淀粉酶降解培养物表面来辅助附着细胞的恢复（分离）。细胞扩增方法包括以下步骤：a）将包含含有胍基团的可降解多糖的细胞，培养基和细胞培养物表面加入到生物反应器中; b）通过贴壁细胞培养来扩增所述细胞; 和c）通过将所述细胞暴露于多糖酶来降低所述培养表面来帮助所述细胞脱离。

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