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1. 发明申请

WO2006047669A9 NON-RANDOM METHOD OF GENE SHUFFLING 审中-公开
公开(公告)号：WO2006047669A9
公开(公告)日：2006-07-20
申请号：PCT/US2005038725
申请日：2005-10-26
申请人： MONSANTO TECHNOLOGY LLC , HAUGE BRIAN M , DONG FENGGAO
发明人： HAUGE BRIAN M , DONG FENGGAO
CPC分类号： C12N15/1031 , C12N15/1027 , C12N15/1093 , C12N15/64 , C12N15/66
摘要： The present invention concerns the non-random assembling of DNA molecules in a DNA construct and methods of using such constructs, including the production of nucleic acid libraries. The non-random gene shuffling is preferably accomplished by the following steps. First, optionally, the amino acid sequences of proteins encoded by related gene families of interest are aligned and inspected for regions of conserved amino acid residues. These conserved regions, preferably of at least 4 (e.g. about 4 to 10) consecutive conserved amino acid residues are candidate regions for the subsequent design of PCR primers to amplify the variable or less conserved regions in between them, followed by non-random reassembly to create a recombinant nucleic acid genetic library of gene family variants.

2. 发明申请

WO2006047669A3 NON-RANDOM METHOD OF GENE SHUFFLING 审中-公开
标题翻译：非随机的基因密封方法
公开(公告)号：WO2006047669A3
公开(公告)日：2006-08-24
申请号：PCT/US2005038725
申请日：2005-10-26
申请人： MONSANTO TECHNOLOGY LLC , HAUGE BRIAN M , DONG FENGGAO
发明人： HAUGE BRIAN M , DONG FENGGAO
IPC分类号： C12N15/10 , C40B40/08 , C40B50/06
CPC分类号： C12N15/1031 , C12N15/1027 , C12N15/1093 , C12N15/64 , C12N15/66
摘要： The present invention concerns the non-random assembling of DNA molecules in a DNA construct and methods of using such constructs, including the production of nucleic acid libraries. The non-random gene shuffling is preferably accomplished by the following steps. First, optionally, the amino acid sequences of proteins encoded by related gene families of interest are aligned and inspected for regions of conserved amino acid residues. These conserved regions, preferably of at least 4 (e.g. about 4 to 10) consecutive conserved amino acid residues are candidate regions for the subsequent design of PCR primers to amplify the variable or less conserved regions in between them, followed by non-random reassembly to create a recombinant nucleic acid genetic library of gene family variants.
摘要翻译：本发明涉及DNA构建体中DNA分子的非随机组装和使用这种构建体的方法，包括产生核酸文库。非随机基因改组优选通过以下步骤完成。首先，任选地，对由相关目的基因家族编码的蛋白质的氨基酸序列进行比对并检查保守氨基酸残基的区域。这些保守区域，优选至少4个（例如约4至10个）连续保守氨基酸残基的保守区域是用于随后设计PCR引物以扩增它们之间的可变或更少保守区域的候选区域，随后是非随机重组至创建基因家族变体的重组核酸遗传文库。

4. 发明专利

AU2771901A Nucleic acid molecules and other molecules associated with soybean cyst nematoderesistance 未知
公开(公告)号：AU2771901A
公开(公告)日：2001-07-24
申请号：AU2771901
申请日：2001-01-05
申请人： MONSANTO TECHNOLOGY LLC
发明人： HAUGE BRIAN M , WANG MING LI , PARSONS JEREMY D , PARNELL LAURENCE D
IPC分类号： C07K14/415 , C12N15/00
摘要： The present invention is in the field of soybean genetics. More specifically, the invention relates to nucleic acid molecules from regions the soybean genome, which are associated with soybean cyst nematode resistance. The invention also relates to proteins encoded by such nucleic acid molecules as well as antibodies capable of recognizing these proteins. The invention also relates to nucleic acid markers from regions the soybean genome, which are associated with soybean cyst nematode resistance. Moreover, the invention relates to uses of such molecules, including, transforming soybean cyst nematode resistant soybean with constructs containing nucleic acid molecules from regions the soybean genome, which are associated with soybean cyst nematode resistance. Furthermore, the invention relates to the use of such molecules in a plant breeding program.

5. 发明专利

CA2396359C NUCLEIC ACID MOLECULES AND OTHER MOLECULES ASSOCIATED WITH SOYBEAN CYST NEMATODE RESISTANCE 未知
公开(公告)号：CA2396359C
公开(公告)日：2019-09-17
申请号：CA2396359
申请日：2001-01-05
申请人： MONSANTO TECHNOLOGY LLC
发明人： HAUGE BRIAN M , WANG MING LI , PARSONS JEREMY D , PARNELL LAURENCE D
IPC分类号： C12N15/82 , C07K14/415 , C12N15/29
摘要： The present invention is in the field of soybean genetics. More specifically, the invention relates to nucleic acid molecules from regions of the soybean genome, which are associated with soybean cyst nematode resistance. The invention also relates to proteins encoded by such nucleic acid molecules as well as antibodies capable of recognizing these proteins. The invention also relates to nucleic acid markers from regions of the soybean genome, which are associated with soybean cyst nematode resistance. Moreover, the invention relates to uses of such molecules, including, transforming soybean cyst nematode resistant soybean with constructs containing nucleic acid molecules from regions of the soybean genome, which are associated with soybean cyst nematode resistance. Furthermore, the invention relates to the use of such molecules in a plant breeding program.

6. 发明专利

BR0107440A 未知
公开(公告)号：BR0107440A
公开(公告)日：2003-03-25
申请号：BR0107440
申请日：2001-01-05
申请人： MONSANTO TECHNOLOGY LLC
发明人： HAUGE BRIAN M , WANG MING LI , PARSONS JEREMY D , PARNELL LAURENCE D
IPC分类号： C07K14/415 , C12N15/00
摘要： The present invention is in the field of soybean genetics. More specifically, the invention relates to nucleic acid molecules from regions the soybean genome, which are associated with soybean cyst nematode resistance. The invention also relates to proteins encoded by such nucleic acid molecules as well as antibodies capable of recognizing these proteins. The invention also relates to nucleic acid markers from regions the soybean genome, which are associated with soybean cyst nematode resistance. Moreover, the invention relates to uses of such molecules, including, transforming soybean cyst nematode resistant soybean with constructs containing nucleic acid molecules from regions the soybean genome, which are associated with soybean cyst nematode resistance. Furthermore, the invention relates to the use of such molecules in a plant breeding program.

7. 发明专利

CA2396359A1 NUCLEIC ACID MOLECULES AND OTHER MOLECULES ASSOCIATED WITH SOYBEAN CYST NEMATODE RESISTANCE 未知
公开(公告)号：CA2396359A1
公开(公告)日：2001-07-19
申请号：CA2396359
申请日：2001-01-05
申请人： MONSANTO TECHNOLOGY LLC
发明人： PARNELL LAURENCE D , HAUGE BRIAN M , PARSONS JEREMY D , WANG MING LI
IPC分类号： C07K14/415 , C12N15/82 , A01H5/10 , C12N15/29
摘要： The present invention is in the field of soybean genetics. More specifically , the invention relates to nucleic acid molecules from regions of the soybean genome, which are associated with soybean cyst nematode resistance. The invention also relates to proteins encoded by such nucleic acid molecules as well as antibodies capable of recognizing these proteins. The invention also relates to nucleic acid markers from regions of the soybean genome, which ar e associated with soybean cyst nematode resistance. Moreover, the invention relates to uses of such molecules, including, transforming soybean cyst nematode resistant soybean with constructs containing nucleic acid molecules from regions of the soybean genome, which are associated with soybean cyst nematode resistance. Furthermore, the invention relates to the use of such molecules in a plant breeding program.

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