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1. 发明申请

WO1998008807A1 SYNTHESIS OF A HYDRAZONE 'beta'-KETO ESTER BY THE REACTION WITH A DIAZO ESTER 审中-公开
标题翻译：通过与DIAZO ESTER的反应合成水解β-酮酯
公开(公告)号：WO1998008807A1
公开(公告)日：1998-03-05
申请号：PCT/US1997015345
申请日：1997-08-29
申请人： MONSANTO COMPANY , SHAH, Ajit, S. , CLARK, Jerry, D. , MA, Yinong , PETERSON, James, C. , PATELIS, Lefteris
发明人： MONSANTO COMPANY
IPC分类号： C07C249/16
CPC分类号： C07D237/24 , C07C249/16 , C07C251/76
摘要： The present invention relates to the synthesis of a hydrazone beta -keto ester by the reaction of an alkyl diazo ester with a hydrazone aldehyde in the presence of a Lewis acid. In a preferred embodiment, the hydrazone beta -keto ester is then converted into a pyridazinone compound by the reaction with an alkyl acid chloride in the presence of a base, followed by acidification. The present invention also relates to a continuous process for the production of a hydrazone aldehyde comprising contacting a hydrazine and glyoxal, wherein the hydrazine and the glyoxal are added simultaneously to a reactor at a controlled rate.
摘要翻译：本发明涉及在路易斯酸存在下，通过烷基重氮酯与腙醛的反应来合成腙β-酮酯。在优选的实施方案中，然后通过在碱存在下与烷基酰氯反应，然后酸化，将腙β-酮酯转化为哒嗪酮化合物。本发明还涉及生产腙醛的连续方法，包括使肼和乙二醛接触，其中肼和乙二醛以受控的速率同时加入到反应器中。

2. 发明公开

EP0781327A1 PRODUCTION OF HYDROXYLATED FATTY ACIDS IN GENETICALLY MODIFIED PLANTS 失效
标题翻译：天然维生素E在天然橡胶中的HERSTELLUNG VON HYDROXYLIERTENFETTSÄUREN
公开(公告)号：EP0781327A1
公开(公告)日：1997-07-02
申请号：EP95934442.0
申请日：1995-09-25
申请人： CARNEGIE INSTITUTION OF WASHINGTON , MONSANTO COMPANY
发明人： CARNEGIE INSTITUTION OF WASHINGTON , MONSANTO COMPANY
IPC分类号： A01H5 , C12N9 , C12N15 , C12P7 , C12Q1
CPC分类号： C12N9/0071 , C12N9/0073 , C12N15/8222 , C12N15/8247
摘要： This invention relates to plant fatty acyl hydroxylases. Methods to use conserved amino acid or nucleotide sequences to obtain plant fatty acyl hydroxylases are described. Also described is the use of cDNA clones encoding a plant hydroxylase to produce a family of hydroxylated fatty acids in transgenic plants.
摘要翻译：本发明涉及植物脂肪酰羟化酶。描述了使用保守氨基酸或核苷酸序列获得植物脂肪酰羟基化酶的方法。还描述了使用编码植物羟化酶的cDNA克隆在转基因植物中产生羟基化脂肪酸家族。

3. 发明申请

WO1999019252A1 METHOD FOR SEPARATING PHOSPHORUS OXYACIDS, ORGANOPHOSPHATES AND ORGANOPHOSPHITES 审中-公开
标题翻译：分离磷氧化物，有机磷酸盐和有机磷酸盐的方法
公开(公告)号：WO1999019252A1
公开(公告)日：1999-04-22
申请号：PCT/US1998021182
申请日：1998-10-08
申请人： MONSANTO COMPANY
发明人： MONSANTO COMPANY , MA, Yinong
IPC分类号： C01B25/16
CPC分类号： C01B25/16 , C01B25/163 , C01B25/165 , C07F9/025 , C07F9/11
摘要： Methods for the preparation and separation of phosphorus oxyacids, organophosphates, and organophosphites are disclosed. Phosphorus species are obtained from the contact of elemental phosphorus, oxygen, and an alcohol. Purification is effected by differential ester hydrolysis and biphasic partitioning. The disclosed methods are performed under mild conditions, and avoid the use of halogenated materials.
摘要翻译：公开了制备和分离含磷酸，有机磷酸酯和有机亚磷酸酯的方法。磷类从元素磷，氧和醇的接触获得。纯化通过差异酯水解和双相分配进行。所公开的方法在温和条件下进行，并避免使用卤化物质。

4. 发明申请

WO1998045457A1 TRANSGENIC PLANTS WITH MODIFIED STEROL BIOSYNTHETIC PATHWAYS 审中-公开
标题翻译：具有改良的固醇生物传播途径的转基因植物
公开(公告)号：WO1998045457A1
公开(公告)日：1998-10-15
申请号：PCT/US1997023495
申请日：1997-12-24
申请人： MONSANTO COMPANY
发明人： MONSANTO COMPANY , NES, W., David
IPC分类号： C12N15/82
CPC分类号： C12N9/1007 , C12N15/8243 , C12N15/8279 , C12N15/8286 , C12P33/00 , Y02A40/162
摘要： Plant phytosterol compositions are modulated in transgenic plants to confer resistance to insects, nematodes, fungi, and/or other environmental stresses, and/or to improve the nutritional value of the plants. Recombinant DNA molecules of the invention encode RNA or protein sequences capable of altering plant sterol profiles by affecting the expression or activity of sterol biosynthetic enzymes. The DNA molecules are transformed into plant cells and plants having altered sterol compositions are regenerated therefrom.
摘要翻译：植物植物甾醇组合物在转基因植物中调节以赋予对昆虫，线虫，真菌和/或其他环境胁迫的抗性，和/或改善植物的营养价值。本发明的重组DNA分子通过影响固醇生物合成酶的表达或活性而编码能够改变植物甾醇分布的RNA或蛋白质序列。将DNA分子转化成植物细胞，并且从其中再生具有改变的固醇组合物的植物。

5. 发明申请

WO1998039453A1 METHODS FOR THE BIOSYNTHESIS OF POLYESTERS 审中-公开
标题翻译：聚酯的生物合成方法
公开(公告)号：WO1998039453A1
公开(公告)日：1998-09-11
申请号：PCT/US1997003994
申请日：1997-03-03
申请人： MONSANTO COMPANY , HEIN, Silke , SÖHLING, Brigitte , GOTTSCHALK, Gerhard , STEINBÜCHEL, Alexander
发明人： MONSANTO COMPANY
IPC分类号： C12N15/52
CPC分类号： C12N9/93 , C12N9/1029 , C12N15/8243 , C12P7/625
摘要： Coexpression of a polyhydroxyalkanoic acid synthase and either a fatty acid: acyl-CoA transferase or an acyl-CoA synthetase in cells enables the biosynthesis of polyester materials. Plasmids, bacteria, materials, and methods for the preparation of polyesters are disclosed.
摘要翻译：聚羟基链烷酸合酶和脂肪酸：酰基-CoA转移酶或酰基-CoA合成酶在细胞中的共表达使得能够生物合成聚酯材料。公开了用于制备聚酯的质粒，细菌，材料和方法。

6. 发明申请

WO1998029131A3 AQUEOUS GLYCEROL FORMULATIONS OF SOMATOTROPIN 审中-公开
公开(公告)号：WO1998029131A3
公开(公告)日：1998-07-09
申请号：PCT/US1997023497
申请日：1997-12-31
申请人： MONSANTO COMPANY
发明人： MONSANTO COMPANY , HEINZ, Daniel, Nicholas
IPC分类号： A61K38/27

7. 发明申请

WO1998018954A1 PROCESS AND COMPOSITION FOR PREPARING D-ASPARTIC ACID 审中-公开
标题翻译：制备D-天冬氨酸的方法和组合物
公开(公告)号：WO1998018954A1
公开(公告)日：1998-05-07
申请号：PCT/US1997019371
申请日：1997-10-28
申请人： MONSANTO COMPANY
发明人： MONSANTO COMPANY , PANTALEONE, David, P. , FOTHERINGHAM, Ian, G. , TON, Jennifer, L.
IPC分类号： C12P41/00
CPC分类号： C12P41/001 , C12P13/06 , C12P13/20
摘要： A process and composition for preparing D-aspartic acid and beta -alanine from D,L-aspartic acid, wherein a solution of D,L-aspartic acid or a salt thereof is contacted with a composition having an L-aspartate- alpha -decarboxylase activity of greater than 100 mu mol L-aspartate used per hour per gram of cells, under appropriate conditions to produce D-aspartic acid and beta -alanine.
摘要翻译：从D，L-天冬氨酸制备D-天冬氨酸和β-丙氨酸的方法和组合物，其中D，L-天冬氨酸或其盐的溶液与具有L-天冬氨酸-α-脱羧酶的组合物接触在适当条件下，每小时每克细胞使用大于100微摩尔L-天冬氨酸的活性，以产生D-天冬氨酸和β-丙氨酸。

8. 发明申请

WO1998017108A1 COMPOSITION AND METHOD FOR TREATING PLANTS WITH EXOGENOUS CHEMICALS 审中-公开
标题翻译：用异黄酮化合物处理植物的组合物和方法
公开(公告)号：WO1998017108A1
公开(公告)日：1998-04-30
申请号：PCT/US1997019540
申请日：1997-10-24
申请人： MONSANTO COMPANY
发明人： MONSANTO COMPANY , WARNER, James, M. , WARD, Anthony, J., I.,
IPC分类号： A01N25/08
CPC分类号： A01N47/24 , A01N25/28 , A01N25/30 , A01N33/22 , A01N35/06 , A01N37/22 , A01N37/40 , A01N39/04 , A01N41/04 , A01N43/40 , A01N43/50 , A01N47/36 , A01N57/20 , Y10S514/975 , A01N25/08 , A01N25/04 , A01N37/48 , A01N37/26 , A01N25/02 , A01N2300/00
摘要： Methods and compositions are disclosed wherein exogenous chemicals are applied to plants to generate a desired biological response. One embodiment of the present invention is a plant treatment composition that comprises (a) a water-soluble exogenous chemical and (b) an adjuvant amount of a solid inorganic particulate colloidal material. Another embodiment of the present invention is an aqueous concentrate plant treatment composition that comprises (a) a water-soluble exogenous chemical, (b) an aqueous diluent, (c) a surfactant component comprising one or more nonionic surfactant(s), and (d) an amount of a solid inorganic particulate colloidal material effective to stabilize the composition, said composition not exhibiting phase separation over a period of time T when stored in a closed container at a temperature in the range from about 15 DEG C to about 30 DEG C; T being in the range from about 1 hour to about 60 days; wherein the exogenous chemical and the surfactant are present at concentrations in the absolute or relative to each other such that, in the absence of the colloidal material, phase separation would occur during said period of time T.
摘要翻译：公开了将外源性化学品施用于植物以产生期望的生物反应的方法和组合物。本发明的一个实施方案是植物处理组合物，其包含（a）水溶性外源性化学品和（b）佐剂量的固体无机微粒胶体材料。本发明的另一个实施方案是含水浓缩植物处理组合物，其包含（a）水溶性外源化学品，（b）水性稀释剂，（c）包含一种或多种非离子表面活性剂的表面活性剂组分和（ d）一定量的有效稳定组合物的固体无机微粒胶体材料，当在约15℃至约30℃的温度下储存在密闭容器中时，所述组合物在一段时间T内不显示相分离 C; T在约1小时至约60天的范围内; 其中外源化学品和表面活性剂以绝对或相对彼此的浓度存在，使得在不存在胶体材料的情况下，在所述时间段T内将发生相分离。

9. 发明申请

WO1998014602A2 PREPARATION OF D-AMINO ACIDS BY DIRECT FERMENTATIVE MEANS 审中-公开
标题翻译：通过直接发酵方法制备D-氨基酸
公开(公告)号：WO1998014602A2
公开(公告)日：1998-04-09
申请号：PCT/US1997017133
申请日：1997-09-25
申请人： MONSANTO COMPANY
发明人： MONSANTO COMPANY , FOTHERINGHAM, Ian, G. , TAYLOR, Paul, P. , TON, Jennifer, L.
IPC分类号： C12P13/04
CPC分类号： C12N9/90 , C12N9/1096 , C12N9/78 , C12P13/04 , C12P13/22
摘要： The present invention relates to materials and methods for production of natural and unnatural D-amino acids. In particular, the present invention relates to a fermentation method for the production of D-amino acids using recombinant host cells. Specifically, the invention relates to a method for producing a D-amino acid in a cell, comprising: (a) incorporating into the cell a D-aminotransferase gene and an L-aminodeaminase gene; (b) culturing the cell in a cell culture medium; and (c) isolating the D-amino acid from the cell culture medium. The invention also relates to a method for producing D-phenylalanine in a cell, comprising: (a) incorporating into the cell a D-aminotransferase gene, an L-aminodeaminase gene and means for increasing production of phenylpyruvate; (b) culturing the cell in a cell culture medium; and (c) isolating the D-phenylalanine from the cell culture medium. The invention also relates to the preparation of recombinant cells for use in the production of enantiomerically pure D-amino acids.
摘要翻译：本发明涉及生产天然和非天然D-氨基酸的材料和方法。特别地，本发明涉及使用重组宿主细胞生产D-氨基酸的发酵方法。具体地说，本发明涉及一种在细胞中产生D-氨基酸的方法，包括：（a）向细胞中掺入D-氨基转移酶基因和L-氨基脱氨酶基因; （b）在细胞培养基中培养细胞; 和（c）从细胞培养基中分离D-氨基酸。本发明还涉及一种在细胞中生产D-苯丙氨酸的方法，其包括：（a）向细胞中掺入D-氨基转移酶基因，L-氨基脱氨酶基因和增加苯丙酮酸生产的方法; （b）在细胞培养基中培养细胞; 和（c）从细胞培养基中分离D-苯丙氨酸。本发明还涉及用于制备对映异构体纯的D-氨基酸的重组细胞的制备。

10. 发明申请

WO1998013723A2 ADDITION OF COLOR TO ELECTROCHROMIC DISPLAYS 审中-公开
标题翻译：添加颜色到电子显示
公开(公告)号：WO1998013723A2
公开(公告)日：1998-04-02
申请号：PCT/US1997017261
申请日：1997-09-26
申请人： MONSANTO COMPANY , COLEMAN, James, P. , LYNCH, Anne, T.
发明人： MONSANTO COMPANY
IPC分类号： G02F01/155
CPC分类号： G02F1/155 , G02F1/1523 , G02F2001/1557
摘要： Disclosed herein is a method of enhancing colors displayed by an electrochromic display. The method involves incorporating an inert pigment into the display. Tin oxide heavily doped with antimony or niobium may be used as electrochromic conducting material.
摘要翻译：本文公开了一种增强由电致变色显示器显示的颜色的方法。该方法包括在显示器中加入惰性颜料。重掺杂有锑或铌的氧化锡可用作电致变色导电材料。

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