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1. 发明申请

WO1997046319A1 HIGH-SPEED LIQUID DEPOSITION DEVICE FOR BIOLOGICAL MOLECULE ARRAY FORMATION 审中-公开
标题翻译：用于生物分子阵列形成的高速液体沉积装置
公开(公告)号：WO1997046319A1
公开(公告)日：1997-12-11
申请号：PCT/US1997009649
申请日：1997-06-03
申请人： MOLECULAR DYNAMICS, INC.
发明人： MOLECULAR DYNAMICS, INC. , ROACH, David, J. , JOHNSTON, Richard, F.
IPC分类号： B01L03/02
CPC分类号： B01L3/0262 , B01J2219/00387 , B01J2219/00527 , B01J2219/00596 , B01J2219/00605 , B01J2219/00639 , B01J2219/00659 , B01J2219/0072 , B01L3/0248 , B01L3/0255 , B01L2300/0838 , C40B60/14 , G01N2035/1037 , Y10T436/25 , Y10T436/2575
摘要： A microspot deposition system (12) featuring a hollow cylindrical wall (26) extending from a closed end (28), terminating in an open end (30) and including a longitudinal gap (34) extending from the open end (30) toward the closed end (28) to allow the rapid exhaustion of the atmosphere and efficient cleaning within the cylindrical wall (26). The cylindrical wall (26) defines a lumen (32) with both the lumen (32) and the gap (34) adapted to facilitate capillary action of liquid in fluid communication therewith to form a meniscus (46) proximate to the open end (30). To facilitate deposition of liquid contained within the lumen (32), the gap (34) may be tapered so that it is narrowest proximate to the open end (30). The narrowed portion of the gap (34) results in a meniscus (46) having a reduced area to ensure preferential fluid flow toward the open end (30), which facilitates deposition via capillary action between the liquid in the lumen (32) and a working surface (52).
摘要翻译：一种微孔沉积系统（12），其具有从封闭端（28）延伸的中空圆柱形壁（26），终止于开口端（30），并且包括从开口端（30）延伸的纵向间隙（34）闭合端（28）以允许气氛的快速耗尽并且在圆柱形壁（26）内进行有效的清洁。圆柱形壁（26）限定内腔（32），内腔（32）和间隙（34）都适于促进与其流体连通的液体的毛细作用，以形成靠近开口端（30）的弯月面（46））。为了便于包含在内腔（32）内的液体的沉积，间隙（34）可以是锥形的，使得其在开口端（30）附近最窄。间隙（34）的变窄的部分导致弯液面（46）具有减小的面积，以确保朝向开口端（30）的优先流体流动，这有利于通过管腔（32）中的液体和工作面（52）。

2. 发明申请

WO1997003350A1 DENATURING SEPARATION MATRIX HAVING HYDROXYETHYL CELLULOSE FOR NUCLEIC ACID ELECTROPHORESIS 审中-公开
标题翻译：用于核酸电泳的去羟基乙酸纤维素分离基质
公开(公告)号：WO1997003350A1
公开(公告)日：1997-01-30
申请号：PCT/US1996010311
申请日：1996-06-14
申请人： MOLECULAR DYNAMICS, INC.
发明人： MOLECULAR DYNAMICS, INC. , BASHKIN, John, S. , BARKER, David, L. , JOHNSTON, Richard, F.
IPC分类号： G01N27/26
CPC分类号： G01N27/44747
摘要： A capillary electrophoresis separation matrix for single-stranded nucleic acids, along with methods for using and preparing the matrix, are disclosed. The separation matrix provides denaturing conditions and contains hydroxyethyl cellulose (HEC) in combination with urea, and preferably also includes formamide. The separation matrix may be used for DNA sizing and sequencing applications and provides a single-base resolution to approximately 500 base pairs. In the figure, line (10) represents peak spacing and line (20) representa peak width of the HEC/urea/formamide separation matrix. Line (15) and line (25) represent the peak spacing and peak width, respectively, of the prior art matrix. The separation matrix is inexpensive, easy to prepare, requires no polymerization steps, and is of low enough viscosity to be pumped easily into and out of capillary tubes for electrophoresis. The low viscosity allows for high throughput of samples and reuse of the capillary tubes for numerous separations.
摘要翻译：公开了用于单链核酸的毛细管电泳分离基质，以及用于使用和制备基质的方法。分离基质提供变性条件并含有与尿素组合的羟乙基纤维素（HEC），并且优选还包括甲酰胺。分离矩阵可用于DNA大小和测序应用，并提供大约500个碱基对的单碱基分辨率。在图中，线（10）表示峰间距，线（20）表示HEC /尿素/甲酰胺分离基质的峰宽。线（15）和线（25）分别代表现有技术矩阵的峰间距和峰宽。分离基质便宜，易于制备，不需要聚合步骤，并且具有足够低的粘度以便容易地泵入和流出用于电泳的毛细管。低粘度允许样品的高通量和用于多次分离的毛细管的再利用。

3. 发明公开

EP0991472A1 HIGH-SPEED LIQUID DEPOSITION DEVICE FOR BIOLOGICAL MOLECULE ARRAY FORMATION 失效
标题翻译：高速液相分离装置用于生化分子的形成DIES
公开(公告)号：EP0991472A1
公开(公告)日：2000-04-12
申请号：EP97928828.9
申请日：1997-06-03
申请人： MOLECULAR DYNAMICS, INC.
发明人： ROACH, David, J. , JOHNSTON, Richard, F.
IPC分类号： B01L3/02 , G01N35/10
CPC分类号： B01L3/0262 , B01J2219/00387 , B01J2219/00527 , B01J2219/00596 , B01J2219/00605 , B01J2219/00639 , B01J2219/00659 , B01J2219/0072 , B01L3/0248 , B01L3/0255 , B01L2300/0838 , C40B60/14 , G01N2035/1037 , Y10T436/25 , Y10T436/2575
摘要： A microspot deposition system (12) featuring a hollow cylindrical wall (26) extending from a closed end (28), terminating in an open end (30) and including a longitudinal gap (34) extending from the open end (30) toward the closed end (28) to allow the rapid exhaustion of the atmosphere and efficient cleaning within the cylindrical wall (26). The cylindrical wall (26) defines a lumen (32) with both the lumen (32) and the gap (34) adapted to facilitate capillary action of liquid in fluid communication therewith to form a meniscus (46) proximate to the open end (30). To facilitate deposition of liquid contained within the lumen (32), the gap (34) may be tapered so that it is narrowest proximate to the open end (30). The narrowed portion of the gap (34) results in a meniscus (46) having a reduced area to ensure preferential fluid flow toward the open end (30), which facilitates deposition via capillary action between the liquid in the lumen (32) and a working surface (52).

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