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1. 发明申请

WO2006101983A2 CHEMICALLY INDUCIBLE EXPRESSION OF BIOSYNTHETIC PATHWAYS 审中-公开
标题翻译：化学途径的化学诱导表达
公开(公告)号：WO2006101983A2
公开(公告)日：2006-09-28
申请号：PCT/US2006009531
申请日：2006-03-16
申请人： METABOLIX INC , KOURTZ LAURALYNN , PEOPLES OLIVER P , SNELL KRISTI D
发明人： KOURTZ LAURALYNN , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： C12N15/82 , A01H5/00 , C12P7/62
CPC分类号： C12N15/8247 , C12N15/8237 , C12N15/8243
摘要： Methods and constructs for the introduction of multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway are provided. In one embodiment, the constructs contain two or more enzyme-encoding genes, each under the control of an inducible promoter and each with a polyadenylation signal. The constructs are used to produce transgenic plants, in which the expression of the enzymes are increased when a chemical inducing agent is applied, and a biosynthetic product of the series of enzymes encoded by the transgenes is produced. Constructs may be used which contain two or more enzyme-encoding genes under the control of one or more promoters activated by activator molecules or complexes expressed from a transgene or transgenes, which are themselves under the control of one or more inducible promoters and switched on following the external application of a chemical. The transgene or transgenes expressing the activator molecules or complexes may be included in the same construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. Alternatively, the transgene or transgenes expressing the activator molecules or complexes may be on a different construct from the construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. The activator molecule can be expressed using a constitutive promoter in an inactive form which is converted to the active form following application of the chemical inducing agent.
摘要翻译：提供了在多酶生物合成途径中引入编码酶的多个基因的方法和构建体。在一个实施方案中，构建体含有两个或更多个酶编码基因，每个基因在诱导型启动子的控制下并且各自具有多聚腺苷酸化信号。构建体用于产生转基因植物，其中当施用化学诱导剂时酶的表达增加，并且产生由转基因编码的一系列酶的生物合成产物。可以使用含有两个或更多个酶编码基因的构建体，所述两个或多个酶编码基因在一个或多个由活化剂分子或由转基因或转基因表达的复合物所激活的启动子的控制下，所述启动子本身处于一个或多个诱导型启动子的控制下，化学品的外部应用。表达活化剂分子或复合物的转基因或转基因可以包含在含有多酶生物合成途径中的酶的多个基因的相同构建体中。或者，表达活化剂分子或复合物的转基因或转基因可以与含有多酶生物合成途径中的酶的多个基因的构建体在不同的构建体上。可以使用非活性形式的组成型启动子来表达活化剂分子，其在施用化学诱导剂之后转化为活性形式。

2. 发明申请

WO2012037324A2 INCREASING CARBON FLOW FOR POLYHYDROXYBUTYRATE PRODUCTION IN BIOMASS CROPS 审中-公开
标题翻译：增加碳水化合物生物质废料中聚羟基丁酸酯的生产
公开(公告)号：WO2012037324A2
公开(公告)日：2012-03-22
申请号：PCT/US2011051728
申请日：2011-09-15
申请人： METABOLIX INC , SOMLEVA MARIYA , TANG JIHONG , ALI AMINAT , BOHMERT-TATAREV KAREN , PATTERSON NII , CHINNAPEN HIMANI , SNELL KRISTI D , PEOPLES OLIVER P
发明人： SOMLEVA MARIYA , TANG JIHONG , ALI AMINAT , BOHMERT-TATAREV KAREN , PATTERSON NII , CHINNAPEN HIMANI , SNELL KRISTI D , PEOPLES OLIVER P
IPC分类号： C12N15/82 , A01H5/12 , C10G3/00 , C10L1/02 , C12P7/62
CPC分类号： C12N15/8261 , C10B53/02 , C10G3/00 , C10G2300/1014 , C10J2300/0916 , C10J2300/092 , C10L5/44 , C12N15/8243 , C12N15/8246 , C12N15/8255 , C12N15/8269 , C12P7/625 , Y02A40/146 , Y02E50/10 , Y02E50/30 , Y02P30/20
摘要： Transgenic plants, transgenic plant material, and transgenic plant cells for the improved synthesis of polyhydroxyalkanoates, preferably poly(3-hydroxybutyrate) (also referred to as PHB), have been developed. In one embodiment, carbon flow is modulated to increase production of PHB. Preferred plants that can be genetically engineered to produce PHB include plants that produce a large amount of lignocellulosic biomass that can be converted into biofuels, such as switchgrass, Miscanthus, Sorghum, sugarcane, millets, Napier grass and other forage and turf grasses. An exemplary plant that can be genetically engineered to produce PHB and produces lignocellulosic biomass is switchgrass, Panicum virgatum L. A preferred cultivar of switchgrass is Alamo. Other suitable cultivars of switchgrass include, but are not limited to, Blackwell, Kanlow, Nebraska 28, Pathfinder, Cave-in-Rock, Shelter and Trailblazer.
摘要翻译：已经开发了转基因植物，转基因植物材料和用于改进合成聚羟基链烷酸酯，优选聚（3-羟基丁酸酯）（也称为PHB））的转基因植物细胞。在一个实施方案中，调节碳流以增加PHB的产生。可以遗传工程化生产PHB的优选植物包括产生大量木质纤维素生物质的植物，其可以转化成生物燃料，例如柳枝稷，芒草，高粱，甘蔗，米勒，纳皮尔草和其他草料和草坪草。可以遗传工程化生产PHB并生产木质纤维素生物质的示范性植物是柳枝稷（Panicum virgatum L.）。优选的柳枝稷品种是Alamo。柳枝稷的其他合适品种包括但不限于：Blackwell，Kanlow，Nebraska 28，Pathfinder，Cave-in-Rock，Shelter和Trailblazer。

3. 发明申请

WO2011034946A1 GENERATION OF HIGH POLYHYDROXYBUTRATE PRODUCING OILSEEDS 审中-公开
标题翻译：高聚羟基丁酸酯生产油脂的生成
公开(公告)号：WO2011034946A1
公开(公告)日：2011-03-24
申请号：PCT/US2010048963
申请日：2010-09-15
申请人： METABOLIX INC , PATTERSON NII , TANG JIHONG , HAN JIXIANG , TAVVA VENKATA , HERTIG ANDREW , ZHANG ZHIGANG , RAMSEIER THOMAS MARTIN , BOHMERT-TATAREV KAREN , PEOPLES OLIVER P , SNELL KRISTI D
发明人： PATTERSON NII , TANG JIHONG , HAN JIXIANG , TAVVA VENKATA , HERTIG ANDREW , ZHANG ZHIGANG , RAMSEIER THOMAS MARTIN , BOHMERT-TATAREV KAREN , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： C12N15/82 , A01H5/00 , C12P7/62
CPC分类号： C12N15/8214 , C12N15/8257
摘要： Transgenic plants, plant material, plant cells, and genetic constructs for synthesis of biopolymers, for example polyhydroxyalkanoates ("PHA") are provided. In one embodiment, the transgenic plants synthesize polyhydroxybutyrate ("PHB"). In one embodiment the transgenic plant encodes siRNA for one or more of the genes encoding enzymes for producing PHA. In a more preferred embodiment, the siRNA expression is under the control of an inducible regulatory element. In another embodiment, the transgenic plant contains transgenes that encode expression enzymes that will degrade the polymer. In a preferred embodiment, the expression of these enzymes is under the control of a germination specific, inducible, or minimal promoter. In another embodiment, the transgenic plant contains transgenes encoding enzymes that increase carbon flow for polymer synthesis. In a preferred embodiment, these transgenes encode enzymes that increase carbon flow in the Calvin Cycle.
摘要翻译：提供了用于合成生物聚合物的转基因植物，植物材料，植物细胞和遗传构建体，例如聚羟基链烷酸酯（“PHA”）。在一个实施方案中，转基因植物合成聚羟基丁酸酯（“PHB”）。在一个实施方案中，转基因植物编码一种或多种编码产生PHA的酶的基因的siRNA。在更优选的实施方案中，siRNA表达处于诱导型调节元件的控制下。在另一个实施方案中，转基因植物含有编码会降解聚合物的表达酶的转基因。在一个优选的实施方案中，这些酶的表达处于发芽特异性，诱导型或最小启动子的控制下。在另一个实施方案中，转基因植物含有编码增加聚合物合成碳流量的酶的转基因。在一个优选的实施方案中，这些转基因编码增加卡尔文循环中碳流量的酶。

4. 发明申请

WO2012170793A8 BIOREFINERY PROCESS FOR THF PRODUCTION 审中-公开
标题翻译：用于生产THF的生物工艺
公开(公告)号：WO2012170793A8
公开(公告)日：2013-03-14
申请号：PCT/US2012041512
申请日：2012-06-08
申请人： METABOLIX INC , VAN WALSEM JOHAN , ANDERSON ERIK , LICATA JOHN , SPARKS KEVIN A , FARMER WILLIAM R , MIRLEY CHRISTOPHER , BICKMEIER JEFFREY A , D AMBRUOSO ANN , SKRALY FRANK , RAMSEIER THOMAS M , SIVASUBRAMANIAN M S , PEOPLES OLIVER P , SHABTAI YOSSEF
发明人： VAN WALSEM JOHAN , ANDERSON ERIK , LICATA JOHN , SPARKS KEVIN A , FARMER WILLIAM R , MIRLEY CHRISTOPHER , BICKMEIER JEFFREY A , D AMBRUOSO ANN , SKRALY FRANK , RAMSEIER THOMAS M , SIVASUBRAMANIAN M S , PEOPLES OLIVER P , SHABTAI YOSSEF
IPC分类号： C12P7/62 , C07D307/08 , C12P17/04
CPC分类号： C12P17/04 , C07D307/08 , C07D307/33 , C07D307/58 , C12P7/625
摘要： Processes and methods for making biobased tetrahydrofuran products from renewable carbon resources are described herein.
摘要翻译：本文描述了从可再生碳资源制备生物基四氢呋喃产物的方法和方法。

5. 发明申请

WO2012149162A3 GREEN PROCESS FOR PRODUCING POLYHYDROXYALKANOATES AND CHEMICALS USING A RENEWABLE FEEDSTOCK 审中-公开
标题翻译：生产聚羟基烷酸酯和化学品的绿色工艺使用可再生料
公开(公告)号：WO2012149162A3
公开(公告)日：2013-01-17
申请号：PCT/US2012035217
申请日：2012-04-26
申请人： METABOLIX INC , ZHANG ZHIGANG , MCCHALICHER CHRISTOPHER W J , VAN WALSEM JOHAN , PEOPLES OLIVER P , ENO RICHARD P , RAMSEIER THOMAS M
发明人： ZHANG ZHIGANG , MCCHALICHER CHRISTOPHER W J , VAN WALSEM JOHAN , PEOPLES OLIVER P , ENO RICHARD P , RAMSEIER THOMAS M
IPC分类号： C12N1/20 , C12N15/52
CPC分类号： C12N15/63 , C12N1/20 , C12N15/52 , C12P7/04 , C12P7/18 , C12P7/24 , C12P7/46 , C12P7/625
摘要： Disclosed are organisms genetically engineered to make useful products when grown on ethanol as a carbon source. The organisms are genetically engineered to produce various useful products such as polyhydroxyalkanoates, diols, diacids, higher alcohols, and other useful chemicals.
摘要翻译：公开的是当在乙醇作为碳源生长时被遗传工程化以制备有用产物的生物。生物体被遗传工程化以产生各种有用的产物，例如聚羟基链烷酸酯，二醇，二酸，高级醇和其它有用的化学品。

6. 发明专利

JP2012072148A Nutritional and therapeutic use of 3-hydroxyalkanoate oligomer 审中-公开
标题翻译： 3-羟基甘氨酸低聚物的营养和治疗用途
公开(公告)号：JP2012072148A
公开(公告)日：2012-04-12
申请号：JP2011231730
申请日：2011-10-21
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： MARTIN DAVID P , PEOPLES OLIVER P , WILLIAMS SIMON F , ZHONG LUHUA
IPC分类号： A61K31/335 , A23K1/16 , A23K1/18 , A23L1/30 , A61K31/00 , A61K31/19 , A61K31/22 , A61K31/23 , A61K31/765 , A61P3/00 , A61P3/04 , A61P3/10 , A61P7/00 , A61P9/00 , A61P21/04 , A61P25/00 , A61P25/08 , A61P25/28 , A61P43/00 , C07C69/675 , C07D323/00
CPC分类号： A61K31/765 , A23K20/105 , A23K50/10 , A23L33/10 , A23V2002/00 , A61K31/00 , A61K31/19 , A23V2250/206 , A23V2250/21 , A23V2250/30
摘要： PROBLEM TO BE SOLVED: To provide a nutritional or a therapeutic composition for increasing ketone body levels in the blood of mammals.SOLUTION: There is provided the nutritional or therapeutic composition for increasing the blood ketone levels during oral ingestion including a 3-hydroxy acid (wherein, the 3-hydroxy acid is not 3-hydroxybutyric acid) as an active ingredient. In the composition, the 3-hydroxy acid is in a form selected from the group consisting of: (a) linear oligomers of 3-hydroxy acids in combination with acetoacetate; (b) cyclic oligolides of the 3-hydroxy acids; (c) esters of the 3-hydroxy acids in combination with the acetoacetate; (d) esters of 3-hydroxy acid linear oligomers and cyclic oligolides in combination with the acetoacetate; and (e) combinations thereof. The composition is useful for increasing cardiotonic effects, treatment of diabetes and insulin resistant states, and treatment of effects of neurodegenerative disorders and epilepsy.
摘要翻译：待解决的问题：提供用于增加哺乳动物血液中酮体水平的营养或治疗组合物。提供了用于在口服摄取期间增加血液酮水平的营养或治疗组合物，包括3-羟基酸（其中，3-羟基酸不是3-羟基丁酸）作为活性成分。在组合物中，3-羟基酸为选自下组的形式：（a）3-羟基酸与乙酰乙酸酯的组合的线性低聚物; （b）3-羟基酸的环状寡聚物; （c）3-羟基酸与乙酰乙酸酯的组合的酯; （d）3-羟基酸线性低聚物和环状寡聚物与乙酰乙酸酯的组合的酯; 和（e）它们的组合。该组合物可用于增加强心作用，治疗糖尿病和胰岛素抵抗状态，以及治疗神经变性疾病和癫痫的作用。版权所有（C）2012，JPO＆INPIT

7. 发明专利

JP2007125043A Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways 审中-公开
标题翻译：从脂肪酸生产途径生产中链长度聚羟基烷酸
公开(公告)号：JP2007125043A
公开(公告)日：2007-05-24
申请号：JP2007035383
申请日：2007-02-15
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： AQUIN STEPHANIE , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H5/00 , C12Q1/44 , A01H1/00 , C12N1/21 , C12N9/00 , C12N9/10 , C12N9/16 , C12N15/09 , C12N15/54 , C12N15/55 , C12N15/82 , C12P7/42 , C12P7/64 , C12Q1/02 , C12Q1/25 , C12Q1/48
CPC分类号： C12N9/93 , C12N9/00 , C12N9/10 , C12N9/1029 , C12N9/16 , C12N15/8243
摘要： PROBLEM TO BE SOLVED: To express an acyl CoA synthetase, a PHA synthase and a PhaG in an organism to produce PHA. SOLUTION: A method for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, is provided. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase (with substrate specificity for medium chain length 3-hydroxy fatty acids), and a medium chain length PHA synthase, is provided. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：在生物体中表达酰基辅酶A合成酶，PHA合酶和PhaG以产生PHA。解决方案：提供了使用3-羟基酰基ACP硫酯酶，PHA合成酶和酰基辅酶A合成酶从脂肪酸生物合成途径制备聚羟基链烷酸酯（PHA）的方法。使用具有3-羟基酰基ACP硫酯酶（酰基辅酶A合成酶（具有中等链长度3-羟基脂肪酸的底物特异性））的酶的非天然细菌PHA生产者和植物中的脂肪酸生物合成途径的PHA生产的方法酸）和中链长度PHA合酶。版权所有（C）2007，JPO＆INPIT

8. 发明专利

JP2008194049A Production of medium chain length polyhydroxyalkanoate from fatty acid biosynthetic pathway 审中-公开
标题翻译：从脂肪酸生物路线生产中链长度聚羟基烷酸
公开(公告)号：JP2008194049A
公开(公告)日：2008-08-28
申请号：JP2008061883
申请日：2008-03-11
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： AQUIN STEPHANIE , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H5/00 , C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/10 , C12N9/16 , C12N15/54 , C12N15/55 , C12N15/82 , C12P7/42 , C12P7/62 , C12Q1/02 , C12Q1/25 , C12Q1/44 , C12Q1/48
CPC分类号： C12N9/93 , C12N9/00 , C12N9/10 , C12N9/1029 , C12N9/16 , C12N15/8243
摘要： PROBLEM TO BE SOLVED: To express PhaG together with acyl CoA synthetase and PHA synthase in organisms in order to carry out production of PHA. SOLUTION: A method for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, has been developed. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants by using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase (with substrate specificity for medium chain length 3-hydroxy fatty acids) and a medium chain length PHA synthase has been developed. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：与生物体中的酰基辅酶A合成酶和PHA合酶一起表达PhaG，以进行PHA的生产。解决方案：已经开发了使用3-羟基酰基ACP硫酯酶，PHA合成酶和酰基辅酶A合成酶从脂肪酸生物合成途径制备聚羟基链烷酸酯（PHA）的方法。通过使用具有3-羟基酰基ACP硫酯酶（酰基辅酶A合成酶）（具有中链长度3-羟基的底物特异性）的催化活性的酶，在非天然细菌PHA生产者和植物中通过脂肪酸生物合成途径产生PHA的方法脂肪酸）和中链长度的PHA合酶。版权所有（C）2008，JPO＆INPIT

9. 发明专利

JP2010213700A Polyhydroxyalkanoate biopolymer composition 审中-公开
标题翻译：聚羟基烷基酯生物聚合物组合物
公开(公告)号：JP2010213700A
公开(公告)日：2010-09-30
申请号：JP2010060249
申请日：2010-03-17
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： SKRALY FRANK A , PEOPLES OLIVER P
IPC分类号： C12N1/21 , C12N15/09 , C08G63/06 , C12N9/00 , C12N9/10 , C12P7/62 , C12R1/19
CPC分类号： C12N9/93 , C08G63/06 , C12N9/1029 , C12P7/625
摘要： PROBLEM TO BE SOLVED: To provide several new PHA (poly[(R)-3-hydroxyalkanoate]) polymer compositions produced using biological systems.
SOLUTION: There is disclosed a means of making polymers accumulate by supplying monomers such as 3-hydroxybutyrate, 3-hydroxypropionate, 2-hydroxybutyrate, 3-hydroxyvalerate, 4-hydroxybutyrate, 4-hydroxyvalerate and 5-hydroxyvalerate as a substrate to cell lines expressing one or more enzymes selected from the group consisting of polyhydroxyalkanoate synthase, acyl-CoA transferase, hydroxyacyl-CoA transferase, and hydroxyacyl-CoA synthetase.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供使用生物系统制备的几种新的PHA（聚[（R）-3-羟基链烷酸酯]）聚合物组合物。解决方案：公开了通过将单体例如3-羟基丁酸酯，3-羟基丙酸酯，2-羟基丁酸酯，3-羟基戊酸酯，4-羟基丁酸酯，4-羟基戊酸酯和5-羟基戊酸酯作为底物而提供聚合物的方法，表达选自聚羟基烷酸酯合成酶，酰基辅酶A转移酶，羟基酰基-CoA转移酶和羟基酰基-CoA合成酶的一种或多种酶的细胞系。版权所有（C）2010，JPO＆INPIT

10. 发明专利

JP2007222185A Production of medium chain length polyhydroxyalkanoate from fatty acid biosynthetic pathway 有权
标题翻译：从脂肪酸生物路线生产中链长度聚羟基烷酸
公开(公告)号：JP2007222185A
公开(公告)日：2007-09-06
申请号：JP2007153418
申请日：2007-06-08
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： AQUIN STEPHANIE , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H5/00 , C12Q1/44 , A01H1/00 , C12N1/21 , C12N9/00 , C12N9/10 , C12N9/16 , C12N15/09 , C12N15/54 , C12N15/55 , C12N15/82 , C12P7/42 , C12Q1/02 , C12Q1/25 , C12Q1/48
CPC分类号： C12N9/93 , C12N9/00 , C12N9/10 , C12N9/1029 , C12N9/16 , C12N15/8243
摘要： PROBLEM TO BE SOLVED: To develop a PhaG with an acyl CoA synthetase and a PHA synthase in an organism so as to produce PHA. SOLUTION: Methods for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways by using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, have been developed. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants by using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase (having substrate specificity for medium chain length 3-hydroxy fatty acids), and a medium chain length PHA synthase, has been developed. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：在生物体中开发具有酰基辅酶A合成酶和PHA合成酶的PhaG，以产生PHA。解决方案：已经开发了通过使用3-羟基酰基ACP硫酯酶，PHA合成酶和酰基辅酶A合成酶从脂肪酸生物合成途径制备聚羟基链烷酸酯（PHA）的方法。通过使用具有3-羟基酰基ACP硫酯酶的催化活性的酶，酰基辅酶A合成酶（具有中链长度3-羟基的底物特异性），使非天然细菌PHA生产者和植物中脂肪酸生物合成途径产生PHA的方法脂肪酸）和中链长度PHA合成酶。版权所有（C）2007，JPO＆INPIT

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