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2. 发明申请

WO2004096831A3 METHOD OF PRODUCING THREE-DIMENSIONAL STRUCTURES USING MOTOR PROTEINS 审中-公开
标题翻译：使用电机蛋白质生产三维结构的方法
公开(公告)号：WO2004096831A3
公开(公告)日：2005-03-03
申请号：PCT/EP2004004630
申请日：2004-04-30
申请人： MAX PLANCK GESELLSCHAFT , UNIV DRESDEN TECH , DIEZ STEFAN , REUTHER CORDULA , HOWARD JONATHON , SEIDEL RALF , MERTIG MICHAEL , POMPE WOLFGANG , BERGMANN MAX
发明人： DIEZ STEFAN , REUTHER CORDULA , HOWARD JONATHON , SEIDEL RALF , MERTIG MICHAEL , POMPE WOLFGANG , BERGMANN MAX
IPC分类号： C07K17/02 , C07K17/00
CPC分类号： B82Y30/00 , C07K17/02
摘要： The present invention relates to a method of producing a three-dimensional structure of a nanornaterial on a surface comprising the step of (a) contacting said polymer material affixed to (aa) biological filaments with biologically active motor proteins carried by said surface; or (ab) biologically active motor proteins with biological filaments carried by said surface in the presence of an energy source in a buffer having a pH value of 5 to 9 and containing monovalent or divalent cations. It is preferred that said nanomaterial is carbon nanotubes or DNA and that said biological filaments are actin filaments or microtubules. It is further preferred that said motor proteins are kinesin or myosin molecules. Also preferred is that said DNA carries metal ions. In particular, the metal ions can be attached to the DNA before or after structure formation. The invention additionally relates to a three-dimensional structure of nanornaterial obtainable by the method of the invention. Said three-dimensional structure preferably is a nanocircuit of metallized DNA.
摘要翻译：本发明涉及一种在表面上制造纳米材料的三维结构的方法，其包括以下步骤：（a）将附着于（aa）生物细丝的所述聚合物材料与由所述表面携带的生物活性运动蛋白接触; 或（ab）生物活性的运动蛋白，其具有在能量源存在下由所述表面承载的具有pH值为5至9并含有一价或二价阳离子的缓冲液中的生物细丝。所述纳米材料优选为碳纳米管或DNA，所述生物细丝为肌动蛋白丝或微管。更优选所述运动蛋白是驱动蛋白或肌球蛋白分子。所述DNA还优选携带金属离子。特别地，金属离子可以在结构形成之前或之后连接到DNA。本发明还涉及通过本发明的方法获得的纳米材料的三维结构。所述三维结构优选为金属化DNA的纳米电路。

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