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1. 发明申请

WO9950427A3 NOVEL BASAL ENDOSPERM TRANSFER CELL LAYER (BETL) SPECIFIC GENES 审中-公开
标题翻译：新型基本内转移细胞层（BETL）特异性基因
公开(公告)号：WO9950427A3
公开(公告)日：1999-12-09
申请号：PCT/EP9902063
申请日：1999-03-26
申请人： MAX PLACK GES ZUR FOERDERUNG D , THOMPSON RICHARD D , YAN GUO , SALAMINI FRANCESCO , HUEROS GREGORIO
发明人： THOMPSON RICHARD D , YAN GUO , SALAMINI FRANCESCO , HUEROS GREGORIO
IPC分类号： A01H5/00 , C07K14/415 , C07K16/16 , C12N5/10 , C12N15/29 , C12N15/82 , G01N33/50
CPC分类号： C12N15/8222 , C07K14/415 , C12N15/8234 , C12N15/8279
摘要： Described are nucleic acid molecules encoding basal endosperm transfer cell layer (BETL) specific proteins as well as regulatory sequences which naturally regulate the expression of such nucleic acid molecules. Vectors comprising said nucleic acid molecules, wherein the nucleic acid molecules are operatively linked to regulatory elements allowing expression in prokaryotic and/or eukaryotic host cells as well as proteins encoded by said nucleic acid molecules, antibodies to said proteins and methods for their production are provided. Described are also recombinant DNA molecules and vectors comprising said regulatory sequences as well as host cells transformed therewith. Furthermore, kits and diagnostic compositions comprising the aforementioned nucleic acid molecules, proteins, antibodies, regulatory sequences, recombinant DNA molecules and vectors as well as antibodies are provided. Also provided are methods for the identification of compounds being capable of activating or inhibiting the expression of BETL specific genes. Furthermore, transgenic plant cells, plant tissue and plants containing the above-described nucleic acid molecules, regulatory sequences, recombinant DNA molecules and vectors as well as the use of the aforementioned nucleic acid molecules, regulatory sequences, recombinant DNA molecules, vectors, proteins, antibodies, peptides and/or compounds identified by a method of the invention in plant cell and tissue culture, plant breeding and/or agriculture are described.
摘要翻译：描述了编码基础胚乳转移细胞层（BETL）特异性蛋白质的核酸分子以及天然调节这种核酸分子表达的调节序列。提供了包含所述核酸分子的载体，其中所述核酸分子可操作地连接到允许在原核和/或真核宿主细胞中表达的调节元件以及由所述核酸分子编码的蛋白质，对所述蛋白质的抗体及其生产方法。还描述了重组DNA分子和包含所述调节序列的载体以及用其转化的宿主细胞。此外，提供了包含上述核酸分子，蛋白质，抗体，调节序列，重组DNA分子和载体以及抗体的试剂盒和诊断组合物。还提供了用于鉴定能够激活或抑制BETL特异性基因表达的化合物的方法。此外，含有上述核酸分子，调节序列，重组DNA分子和载体的转基因植物细胞，植物组织和植物以及上述核酸分子，调节序列，重组DNA分子，载体，蛋白质，描述了通过本发明的方法在植物细胞和组织培养，植物育种和/或农业中鉴定的抗体，肽和/或化合物。

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