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1. 发明申请

WO2007087377A2 PHOTOELECTROCHEMICAL SYNTHESIS OF HIGH DENSITY COMBINATORIAL POLYMER ARRAYS 审中-公开
标题翻译：高密度组合聚合物阵列的光电化学合成
公开(公告)号：WO2007087377A2
公开(公告)日：2007-08-02
申请号：PCT/US2007/001976
申请日：2007-01-25
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , EMIG, Christopher, J. , CHOW, Brian, Y. , JACOBSON, Joseph, M.
发明人： EMIG, Christopher, J. , CHOW, Brian, Y. , JACOBSON, Joseph, M.
IPC分类号： C12Q1/68 , C12M3/00 , H01L21/00
CPC分类号： C25B3/00 , B01J19/0046 , B01J2219/00317 , B01J2219/00432 , B01J2219/00439 , B01J2219/00441 , B01J2219/00448 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00635 , B01J2219/00637 , B01J2219/00641 , B01J2219/00653 , B01J2219/00659 , B01J2219/00675 , B01J2219/00711 , B01J2219/00713 , B01J2219/00722 , B82Y30/00 , C12Q1/6837 , C40B50/14 , C40B60/14 , H01L27/148
摘要： In a method for creating polymer arrays through photoelectrochemically modulated acid/base/radical generation for combinatorial synthesis, electrochemical synthesis is guided by a spatially modulated light source striking a semiconductor in an electrolyte solution. A substrate having at its surface at least one photoelectrode that is proximate to at least one molecule bearing at least one chemical functional group is provided, along with a reagent-generating chemistry co-localized with the chemical functional group and capable of generating reagents when subjected to a potential above a threshold. An input potential is then applied to the photoelectrode that exceeds the threshold in the presence of light and that does not exceed the threshold in the absence of light, causing the transfer of electrons to or from the substrate, and creating a patterned substrate. The process is repeated until a polymer array of desired size is created.
摘要翻译：在通过光电化学调制的酸/碱/自由基产生用于组合合成的聚合物阵列的制备方法中，电化学合成由在电解质溶液中冲击半导体的空间调制光源引导。提供了在其表面上具有至少一个接近至少一个具有至少一个化学官能团的分子的光电极，以及与化学官能团共定位的试剂产生化学物质，并且当经受达到高于阈值的潜力。然后在存在光的情况下将输入电位施加到超过阈值的光电极，并且在不存在光的情况下不超过阈值，导致电子传递到衬底或从衬底转移，并产生图案化衬底。重复该过程，直到产生所需尺寸的聚合物阵列。

2. 发明申请

WO2005059097A2 METHODS FOR HIGH FIDELITY PRODUCTION OF LONG NUCLEIC ACID MOLECULES 审中-公开
标题翻译：高保真度生产长核酸分子的方法
公开(公告)号：WO2005059097A2
公开(公告)日：2005-06-30
申请号：PCT/US2004/041478
申请日：2004-12-10
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , CARR, Peter, A. , CHOW, Brian, Y. , JACOBSON, Joseph, M.
发明人： CARR, Peter, A. , CHOW, Brian, Y. , JACOBSON, Joseph, M.
IPC分类号： C12N
CPC分类号： C12P19/34 , C12Q1/6825 , C12Q1/6834 , C12Q1/6848 , C12Q2565/518 , C12Q2565/513 , C12Q2521/514
摘要： This invention generally relates to nucleic acid synthesis, in particular DNA synthesis. More particularly, the invention relates to the production of long nucleic acid molecules with precise user control over sequence content. This invention also relates to the prevention and/or removal of erros within nucleic acid molecules.
摘要翻译：本发明一般涉及核酸合成，特别是DNA合成。更具体地说，本发明涉及具有精确的用户控制序列含量的长核酸分子的生产。本发明还涉及预防和/或除去核酸分子内的错误。

3. 发明申请

WO2005059096A2 METHODS FOR HIGH FIDELITY PRODUCTION OF LONG NUCLEIC ACID MOLECULES WITH ERROR CONTROL 审中-公开
标题翻译：用于高错误生产具有错误控制的长核酸分子的方法
公开(公告)号：WO2005059096A2
公开(公告)日：2005-06-30
申请号：PCT/US2004/041268
申请日：2004-12-10
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , CARR, Peter, A. , CHOW, Brian, Y. , JACOBSON, Joseph, M.
发明人： CARR, Peter, A. , CHOW, Brian, Y. , JACOBSON, Joseph, M.
IPC分类号： C12N
CPC分类号： B01J19/0046 , B01J2219/00608 , B01J2219/00659 , B01J2219/00722 , C12Q1/6827 , C12Q1/6837 , C12Q1/6846 , C12Q1/6848 , C12Q2565/518 , C12Q2537/149 , C12Q2521/514 , C12Q2537/113 , C12Q2565/537 , C12Q2565/513
摘要： This invention generally relates to nucleic acid synthesis, in particular DNA synthesis. More particularly, the invention relates to the production of long nucleic acid molecules with precise user control over sequence content. This invention also relates to the prevention and/or removal of errors within nucleic acid molecules.
摘要翻译：本发明一般涉及核酸合成，特别是DNA合成。更具体地，本发明涉及具有对序列内容的精确用户控制的长核酸分子的生产。本发明还涉及预防和/或去除核酸分子中的错误。

4. 发明申请

WO2007137242A2 MICROFLUIDIC-BASED GENE SYNTHESIS 审中-公开
标题翻译：基于微流体的基因合成
公开(公告)号：WO2007137242A2
公开(公告)日：2007-11-29
申请号：PCT/US2007/069404
申请日：2007-05-21
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , KONG, David , CARR, Peter, A. , JACOBSON, Joseph, M.
发明人： KONG, David , CARR, Peter, A. , JACOBSON, Joseph, M.
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N15/902 , C12N15/70 , C12P19/34
摘要： A method for synthesizing long DNA constructs from oligonucleotide precursors directly within a microfluidic device uses several oligonucleotides at once. A precursor mix containing at least two oligonucleotide precursors with at least partial base complementarity is introduced into an input of a microfluidic chip and at least one cycle of at least one gene synthesis protocol are applied to fabricate a DNA construct containing the sequence of at least two oligonucleotide precursors. A method for the synthesis of a modified DNA construct includes electroporating at least one oligonucleotide encoding for at least one point mutation and having homology with at least one DNA region of a target cell into the target cell and incorporating the oligonucleotide into the target cell DNA through the action of recombination protein beta or a recombination protein beta functional homolog.
摘要翻译：在微流体装置内直接合成来自寡核苷酸前体的长DNA构建体的方法一次使用若干寡核苷酸。将包含至少两种具有至少部分碱基互补性的寡核苷酸前体的前体混合物引入微流体芯片的输入中，并且至少一个基因合成方案的至少一个循环被应用于制备含有至少两个序列的DNA构建体寡核苷酸前体。用于合成修饰的DNA构建体的方法包括将编码至少一个点突变的至少一种寡核苷酸电穿孔并与靶细胞的至少一个DNA区域具有同源性，并将寡核苷酸并入靶细胞DNA中，通过重组蛋白β或重组蛋白β功能同源物的作用。

5. 发明申请

WO2015070062A1 CELL-BASED GENOMIC RECORDED ACCUMULATIVE MEMORY 审中-公开
标题翻译：基于细胞的基因记录记忆累积记忆
公开(公告)号：WO2015070062A1
公开(公告)日：2015-05-14
申请号：PCT/US2014/064623
申请日：2014-11-07
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人： JACOBSON, Joseph, M. , JAKIMO, Noah , KANAREK, Naama , SABATINI, David
IPC分类号： G06G99/00
CPC分类号： C12Q1/6874 , C12N9/22 , C12N15/102 , C12N15/63 , C12N15/70 , C12Q1/6897
摘要： The present invention relates to a cell based genomic Recorded Accumulative Memory (geRAM) system (also referred to herein as Genomically Encoded Memory (GEM)) for recoding data (i.e., changes in nucleic acid sequences in cellular DNA in response to physical and/or chemical signal(s)) from the cellular environment. The cell-based data sensing and recording system of the invention comprises at least one nucleic acid sequence encoding at least one directed endonuclease specific to at least one target sequence located within the cell wherein the at least one directed endonuclease is operably linked to at least one inducible promoter, wherein the at least one inducible promoter is induced by at least one signal originating from outside of the cell.
摘要翻译：本发明涉及用于重新编码数据的基于细胞的基因组记录累积存储器（geRAM）系统（本文中也称为基因编码存储器（GEM）））（即，响应于物理和/或数据的细胞DNA中核酸序列的变化）化学信号）。本发明的基于细胞的数据感测和记录系统包含至少一个编码至少一个定向内切核酸酶的核酸序列，其至少一个位于细胞内的靶序列特异性，其中所述至少一个定向内切核酸酶可操作地连接至至少一个诱导型启动子，其中所述至少一种诱导型启动子是由来自细胞外的至少一种信号诱导的。

6. 发明申请

WO1998019208A2 NONEMISSIVE DISPLAYS AND PIEZOELECTRIC POWER SUPPLIES THEREFOR 审中-公开
标题翻译：非易失性显示器和压电电源
公开(公告)号：WO1998019208A2
公开(公告)日：1998-05-07
申请号：PCT/US1997018643
申请日：1997-10-17
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , JACOBSON, Joseph, M. , COMISKEY, Barrett
IPC分类号： G02F01/167
CPC分类号： G01C22/006 , B41J3/4076 , G02F1/13306 , G02F1/167 , G08B5/226
摘要： An electrophoretic display has a substantially two-dimensional arrangement of microcapsules each having therein an electrophoretic composition of a dielectric fluid and a suspension of particles that visually contrast with the dielectric liquid and also exhibit surface charges; a pair of electrodes, at least one of which is visually transparent, disposed on and covering opposite sides of the microcapsule arrangement; and means for creating a potential difference between the two electrodes, the potential difference causing the particles to migrate toward one of the electrodes. The display may be powered by one or more piezoelectric elements, which are also suitable for powering other types of nonemissive displays.
摘要翻译：电泳显示器具有基本上二维排列的微胶囊，其中各自具有介电流体的电泳组合物和与电介质液体目视对比并且还表现出表面电荷的颗粒悬浮液; 一对电极，其中至少一个是视觉上透明的，设置在微胶囊装置的相对侧上并覆盖其上; 以及用于产生两个电极之间的电位差的装置，导致颗粒朝着一个电极迁移的电位差。显示器可以由一个或多个压电元件供电，这些压电元件也适用于为其他类型的非发射显示器供电。

7. 发明申请

WO2019217336A3 APPLICATIONS OF STREPTOCOCCUS-DERIVED Cas9 NUCLEASSES ON MINIMAL ADENINE-RICH PAM TARGETS 审中-公开
公开(公告)号：WO2019217336A3
公开(公告)日：2019-11-14
申请号：PCT/US2019/030958
申请日：2019-05-06
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人： CHATTERJEE, Pranam , JAKIMO, Noah, Michael , JACOBSON, Joseph, M. , NIP, Lisa
IPC分类号： A61K48/00 , A61K38/46 , C12N15/00 , C12N15/87 , C07K14/00 , C12N5/02
摘要： Applications of a Streptococcus Cas9 ortholog from Streptococcus macacae (Smac Cas9), possessing minimal adenine-rich PAM specificity, include an isolated Streptococcus macacae Cas9 protein or transgene expression thereof, a CRISPR-associated DNA endonuclease with PAM interacting domain amino acid sequences that are at least 80% identical to that of the isolated Streptococcus macacae Cas9 protein, and an isolated, engineered Streptococcus pyogenes Cas9 (Spy Cas9) protein with a PID as either the PID amino acid composition of the isolated Streptococcus macacae Cas9 (Smac Cas9) protein or of a CRISPR-associated DNA endonuclease with PID amino acid sequences that are at least 80% identical to that of the isolated Streptococcus macacae Cas9 protein. A method for altering expression of at least one gene product employs Streptococcus macacae Cas9 endonucleases in complex with guide RNA, for specific recognition and activity on a DNA target immediately upstream of either an "NAA" or "NA" or "NAAN" PAM sequence.

8. 发明申请

WO2015168404A1 TOEHOLD-GATED GUIDE RNA FOR PROGRAMMABLE CAS9 CIRCUITRY WITH RNA INPUT 审中-公开
标题翻译：用于可编程CAS9电路与RNA输入的TOEHOLD-GATED指南RNA
公开(公告)号：WO2015168404A1
公开(公告)日：2015-11-05
申请号：PCT/US2015/028514
申请日：2015-04-30
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人： JACOBSON, Joseph, M. , JAKIMO, Noah
IPC分类号： C12N9/22
CPC分类号： C12N15/10 , C12N15/111 , C12N2310/10 , C12N2310/20 , C12N2310/53 , C12N2320/11
摘要： The present invention relates to a toehold-gated guide RNA (thgRNA) as described herein and the use of thgRNA in cell based bimolecular circuitry to sense, report, and respond to the transcriptome state of the cellular environment. The present invention, referred to as toehold-gated gRNA (thgRNA) adds nucleotide sequences to the 3' and/or 5' ends of a gRNA, or within the scaffold of a gRNA, such that complementary sequences hybridize to form a stem domain in the secondary structure that constrains the distance between spacer and scaffold domains and sterically protects toehold-gated gRNA from activity with Cas9.
摘要翻译：本发明涉及如本文所述的toehold-gated引导RNA（thgRNA），以及thgRNA在基于细胞的双分子电路中用于感测，报告和响应细胞环境转录组状态的用途。被称为止痛门gRNA（thgRNA）的本发明将核苷酸序列添加到gRNA的3'末端和/或5'末端，或在gRNA的支架内，使得互补序列杂交以形成茎结构域限制间隔区和支架结构域之间的距离的二级结构，并且通过Cas9的空间保护去保护门控gRNA免受活性。

9. 发明授权

EP0937278B1 NONEMISSIVE DISPLAYS AND PIEZOELECTRIC POWER SUPPLIES THEREFOR 失效
标题翻译： NICHTMITTIERENDE节目，其压电电源
公开(公告)号：EP0937278B1
公开(公告)日：2003-09-17
申请号：EP97910132.6
申请日：1997-10-17
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人： JACOBSON, Joseph, M. , COMISKEY, Barrett
IPC分类号： G02F1/167 , G02F1/133
CPC分类号： G01C22/006 , B41J3/4076 , G02F1/13306 , G02F1/167 , G08B5/226

10. 发明申请

WO2022266268A2 APPLICATIONS OF RECOMBINED STREPTOCOCCUS CANIS CAS9 ENZYMES FOR PAM-FREE DNA MODIFICATION 审中-公开
公开(公告)号：WO2022266268A2
公开(公告)日：2022-12-22
申请号：PCT/US2022/033696
申请日：2022-06-15
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人： CHATTERJEE, Pranam , JACOBSON, Joseph, M.
IPC分类号： C07K14/315 , C12N9/22 , C12N15/85 , C07K2319/80 , C12N15/79 , C12N2310/20
摘要： SpRYc is a grafted ScCas9++ - SpRY chimeric Cas9 possessing minimal 5'- NNN-3' PAM specificity. SpRYc comprises the N-terminus (residues 1-1119) of ScCas9++ (Sc++), including the flexible loop, followed by the region of SpRY (residues 1111-1368) spanning its PAM-interacting domain mutations. Methods of altering gene expression include use of SpRYc in complex with guide RNA in a CRISPR-Cas9 system.

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