专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP2361311A4 YEAST EXPRESSING CELLULASES FOR SIMULTANEOUS SACCHARIFICATION AND FERMENTATION USING CELLULOSE 有权转让
标题翻译： HEFEEXPRIMIERENDE纤维素酶用于糖化和皮肤BY CELLULSE
公开(公告)号：EP2361311A4
公开(公告)日：2013-03-27
申请号：EP09828362
申请日：2009-11-23
申请人： MASCOMA CORP , UNIV STELLENBOSCH
发明人： MCBRIDE JOHN , BREVNOVA ELENA , GHANDI CHHAYAL , MELLON MARK , FROEHLICH ALAN , DELEAULT KRISTEN , RAJGARHIA VINEET , FLATT JIM , VAN ZYL EMILE , DEN HAAN RIAAN , LAGRANGE DANIE , ROSE SHAUNITA , PENTTILA MERJA , ILMEN MARJA , SIIKA-AHO MATTI , UUSITALO JAANA , HAU HEIDI HANSON , RICE CHARLES , VILLARI JEFF , STONEHOUSE EMILY A
IPC分类号： C12N9/42 , C12N15/52 , C12P7/06
CPC分类号： C12P7/10 , C12N9/2437 , C12N9/2445 , C12N15/80 , C12P7/14 , C12P39/00 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17

2. 发明申请

WO2010105194A2 MESOPHILIC AND THERMOPHILIC ORGANISMS, AND METHODS OF USE THEREOF 审中-公开
标题翻译：化学和热塑性有机物及其使用方法
公开(公告)号：WO2010105194A2
公开(公告)日：2010-09-16
申请号：PCT/US2010027190
申请日：2010-03-12
申请人： MASCOMA CORP , SHAW ARTUR J , RAJGARHIA VINEET
发明人： SHAW ARTUR J , RAJGARHIA VINEET
IPC分类号： C12N15/63
CPC分类号： C12P7/40 , C12N9/0004 , C12N9/1029 , C12N9/1217 , C12N15/63 , C12P1/04 , C12R1/01
摘要： The present invention provides for novel metabolic pathways leading to acrylate formation in a consolidated bioprocessing system (CBP) where lignocellulosic biomass is efficiently converted to acrylate. In one such metabolic pathway, pyruvate is converted to lactate, which is converted to lactoyol-CoA, which is converted to acryloyl-CoA, and which is finally converted to acrylate. hi another such metabolic pathway, pyruvate is converted to L-a- alanine, which is converted to L-aspartate, which is converted to ß-alanine, which is converted to ß-alanyl-CoA, which is converted to acryloyl-CoA, and which is finally converted to acrylate. In yet another metabolic pathway, pyruvate is converted to lactate, and then lactate is converted directly to acrylate. In certain aspects, the invention provides for heterologous expression of one or more enzymes in a mesophilic or thermophilic organism, such as Thermoanaerobacterium saccharolyticum or Clostridium thermocellutn, where the one or more enzymes functions within a novel metabolic pathway as described above to convert pyruvate to acrylate via lactate, or via ß alanine and acryloyl-CoA.
摘要翻译：本发明提供了在固结生物处理系统（CBP）中导致丙烯酸酯形成的新型代谢途径，其中木质纤维素生物质被有效地转化为丙烯酸酯。在一个这样的代谢途径中，将丙酮酸转化成乳酸盐，将其转化为乳糖醇-CoA，将其转化为丙烯酰辅酶A，并最后转化为丙烯酸酯。在另一个这样的代谢途径中，丙酮酸转化成L-丙氨酸，转化成L-天冬氨酸，转化为β-丙氨酸，转化成β-丙氨酰辅酶A，转化成丙烯酰辅酶A，其中最终转化成丙烯酸酯。在另一种代谢途径中，将丙酮酸转化成乳酸，然后将乳酸酯直接转化成丙烯酸酯。在某些方面，本发明提供了一种或多种酶在嗜温或嗜热生物体中的异源表达，所述生物体如热解酵母分解酶或热梭菌（Clostridium thermocellutn），其中一种或多种酶在如上所述的新型代谢途径中起作用以将丙酮酸转化成丙烯酸酯通过乳酸盐，或通过β丙氨酸和丙烯酰辅酶A。

3. 发明申请

WO2010056805A3 GENE KNOCKOUT MESOPHILIC AND THERMOPHILIC ORGANISMS, AND METHODS OF USE THEREOF 审中-公开
标题翻译：基因克隆夫妇和热休克有机体及其使用方法
公开(公告)号：WO2010056805A3
公开(公告)日：2010-07-08
申请号：PCT/US2009064128
申请日：2009-11-12
申请人： MASCOMA CORP , HOGSETT DAVID A , RAJGARHIA VINEET , SHAW IV ARTHUR J , CAIAZZA NICKY C
发明人： HOGSETT DAVID A , RAJGARHIA VINEET , SHAW IV ARTHUR J , CAIAZZA NICKY C
IPC分类号： C07H21/02 , C12N1/20 , C12N9/04 , C12N9/10 , C12N9/12 , C12N9/88 , C12N9/90 , C12N9/92 , C12N15/00 , C12P7/06 , C12R1/01
CPC分类号： C12P7/54 , C12N9/0006 , C12N9/1029 , C12N9/1205 , C12N9/1217 , C12N9/88 , C12N9/90 , C12N9/92 , C12P7/065 , C12P7/56 , Y02E50/17
摘要： One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce lactate or acetate as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of lactate or acetate. Another aspect of the invention relates to a process for converting lignocellulosic biomass to lactate or acetate, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.
摘要翻译：本发明的一个方面涉及经遗传修饰的嗜热或嗜温微生物，其中第一天然基因部分，基本上或完全缺失，沉默，失活或下调，所述第一天然基因编码参与代谢产生有机酸或其盐，从而增加所述嗜热或嗜温微生物产生作为发酵产物的乳酸盐或乙酸盐的天然能力。在某些实施方案中，上述微生物还包含第一非天然基因，其第一非天然基因编码参与乳酸盐或乙酸盐的代谢产生的第一非天然酶。本发明的另一方面涉及将木质纤维素生物质转化为乳酸盐或乙酸盐的方法，包括使木质纤维素生物质与遗传修饰的嗜热或嗜温微生物接触。

4. 发明申请

WO2010005551A3 HETEROLOGOUS EXPRESSION OF TERMITE CELLULASES IN YEAST 审中-公开
标题翻译：酵母细胞在异育银鲫中的异源表达
公开(公告)号：WO2010005551A3
公开(公告)日：2010-03-18
申请号：PCT/US2009003970
申请日：2009-07-07
申请人： MASCOMA CORP , BREVNOVA ELENA E , RAJGARHIA VINEET , MELLON MARK , WARNER ANNE , MCBRIDE JOHN , GANDHI CHHAYAL , WISWALL ERIN
发明人： BREVNOVA ELENA E , RAJGARHIA VINEET , MELLON MARK , WARNER ANNE , MCBRIDE JOHN , GANDHI CHHAYAL , WISWALL ERIN
IPC分类号： C07K14/435 , C12N1/16 , C12N9/42 , C12N15/81
CPC分类号： C12N9/2437 , C12N15/81 , C12N15/815 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： The present invention provides for heterologous expression of termite and termite- associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
摘要翻译：本发明提供了白蚁和白蚁相关共生体纤维素酶的异源表达。例如，纤维素酶可以是密码子优化的并在酵母宿主细胞如酵母酿酒酵母中表达。纤维素酶也可以与其他纤维素酶在宿主细胞中共表达。在这种宿主细胞中表达白蚁和白蚁相关共生体纤维素酶及其变体和组合的表达导致具有改善的纤维素活性的酵母。因此，这样的基因和表达系统对于有效且成本有效的整合生物处理系统是有用的。

5. 发明专利

CA2772081A1 PRODUCTION OF PROPANOLS, ALCOHOLS, AND POLYOLS IN CONSOLIDATED BIOPROCESSING ORGANISMS 未知
公开(公告)号：CA2772081A1
公开(公告)日：2011-02-24
申请号：CA2772081
申请日：2010-08-20
申请人： MASCOMA CORP
发明人： MCBRIDE JOHN , RAJGARHIA VINEET , SHAW ARTHUR J IV , TRIPATHI SHITAL A , BREVNOVA ELENA , CAIAZZA NICKY , VAN DIJKEN HANS , FROEHLICH ALLAN , SILLERS WILLIAM RYAN , FLATT JIM
IPC分类号： C12P7/00 , C12N15/52 , C12P7/08 , C12P7/20
摘要： The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, upregulated or downregulated.

6. 发明专利

CA2729924C ISOLATION AND CHARACTERIZATION OF SCHIZOCHYTRIUM AGGREGATUM CELLOBIOHYDROLASE I (CBH 1) 未知
公开(公告)号：CA2729924C
公开(公告)日：2018-05-29
申请号：CA2729924
申请日：2009-07-07
申请人： MASCOMA CORP
发明人： BREVNOVA ELENA E , FLATT JIM , GANDHI CHHAYAL , RAJGARHIA VINEET , MCBRIDE JOHN , WARNER ANNE
IPC分类号： C12N9/42
摘要： The present invention provides for the isolation and characterization of the cbhl gene from Schizochytrium aggre-gatum. In particular, the present invention provides for the nucleic acid and amino acid sequences of Schizochytrium aggregatum cbhl, and domains, variants and derivatives thereof. The present invention further provides for the heterologous expression of Schizochytrium aggregatum Cbhl in host cells, including yeast, e.g., Saccharomyces cerevisiae. Expression of Schizochytrium aggregatum Cbhl in host cells will augment cellulose digestion and facilitate ethanol production by those host cells on cellulosic substrates. In certain embodiments, heterologous expression in Saccharomyces cerevisiae is in coordination with heterologous ex-pression of other known, or newly identified saccharolytic enzymes. Therefore, the present invention also provides that the novel Schizochytrium aggregatum Cbhl gene can utilized in a consolidated bioprocessing system.

7. 发明专利

BR112012003883A2 Produção de propanóis álcoois e polióis em organismos de bioprocessamento consolidados. 未知
公开(公告)号：BR112012003883A2
公开(公告)日：2015-09-01
申请号：BR112012003883
申请日：2010-08-20
申请人： MASCOMA CORP
发明人： MCBRIDE JOHN , RAJGARHIA VINEET , SHAW ARTHUR J , IV , TRIPATHI SHITAL A , BREVNOVA ELENA , CAIAZZA NICHY , DIJKEN HANS VAN , FROEHLICH ALLAN , SILLERS WILLIAM RYAN , FLATT JIM
IPC分类号： C12P7/00 , C12N15/52 , C12P7/08 , C12P7/20

8. 发明专利

CA2752976C YEAST CELLS EXPRESSING AN EXOGENOUS CELLULOSOME AND METHODS OF USING THE SAME 未知
公开(公告)号：CA2752976C
公开(公告)日：2018-08-28
申请号：CA2752976
申请日：2010-02-18
申请人： MASCOMA CORP , UNIV STELLENBOSCH
发明人： MCBRIDE JOHN , MELLON MARK , RAJGARHIA VINEET , BREVNOVA ELENA E , WISWALL ERIN , HOGSETT DAVID A , LAGRANGE DANIE , ROSE SHAUNITA , VAN ZYL EMILE
IPC分类号： C12P7/10 , C07K14/33 , C12N9/42 , C12N15/56 , C12N15/62
摘要： The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.

9. 发明专利

ZA201100148B HETEROLOGOUS EXPRESSION OF TERMITE CELLULASES IN YEAST 未知
公开(公告)号：ZA201100148B
公开(公告)日：2020-12-23
申请号：ZA201100148
申请日：2011-01-05
申请人： MASCOMA CORP
发明人： BREVNOVA ELENA E , RAJGARHIA VINEET , MELLON MARK , WARNER ANNE , MCBRIDE JOHN , GANDHI CHHAYAL , WISWALL ERIN
摘要： The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

10. 发明专利

NZ598374A Production of propanols, alcohols, and polyols in consolidated bioprocessing organisms 未知
公开(公告)号：NZ598374A
公开(公告)日：2014-10-31
申请号：NZ59837410
申请日：2010-08-20
申请人： MASCOMA CORP
发明人： SILLERS WILLIAM RYAN , TRIPATHI SHITAL A , SHAW ARTHUR J IV , BREVNOVA ELENA , RAJGARHIA VINEET , FLATT JIM , FROEHLICH ALLAN , MCBRIDE JOHN , CAIAZZA NICKY , VAN DIJKEN HANS
IPC分类号： C12P7/00 , C12N15/52 , C12P7/08 , C12P7/20
摘要： Disclosed is a recombinant microorganism; wherein said microorganism expresses one or more native and/or heterologous enzymes; wherein said one or more enzymes function in one or more engineered metabolic pathways to convert a carbohydrate source to 1,2-propanediol, isopropanol and ethanol or glycerol; and wherein said one or more native and/or heterologous enzymes is activated, upregulated or downregulated.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式