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1. 发明申请

US20100036174A1 BUTANOL PRODUCTION IN A EUKARYOTIC CELL 审中-公开
标题翻译：丁醇生产在一个欧洲细胞
公开(公告)号：US20100036174A1
公开(公告)日：2010-02-11
申请号：US12447740
申请日：2007-10-30
申请人： Lourina Madeleine Raamsdonk , Wilhelmus Theodorus Antonius Maria De Laat , Marco Alexander Van Den Berg
发明人： Lourina Madeleine Raamsdonk , Wilhelmus Theodorus Antonius Maria De Laat , Marco Alexander Van Den Berg
IPC分类号： C12P7/16 , C12N1/16 , C07C31/12
CPC分类号： C12P7/16 , C12N1/18 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/1029 , C12N9/88 , C12Y102/0101 , C12Y103/99002 , C12Y203/01009 , Y02E50/10
摘要： The present invention relates to a transformed eukaryotic cell comprising one or more nucleotide sequence(s) encoding acetyl-CoA acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, 3-hydroxybutyryl-CoA dehydratase, butyryl-CoA dehydrogenase, alcohol dehydrogenase or acetaldehyde dehydrogenase and/or NAD(P)H-dependent butanol dehydrogenase, whereby the nucleotide sequence(s) upon transformation of the cell confer(s) the cell the ability to produce butanol. The invention also relates to a process for the production of butanol.
摘要翻译：本发明涉及包含编码乙酰辅酶A乙酰转移酶，3-羟基丁酰辅酶A脱氢酶，3-羟基丁酰辅酶A脱水酶，丁酰辅酶A脱氢酶，醇脱氢酶或乙醛脱氢酶的一个或多个核苷酸序列的转化真核细胞和/ 或NAD（P）H依赖性丁醇脱氢酶，由此转化细胞后的核苷酸序列赋予细胞产生丁醇的能力。本发明还涉及生产丁醇的方法。

2. 发明申请

US20090233287A1 PRODUCTION OF COMPOUNDS IN A RECOMBINANT HOST 审中-公开
标题翻译：在重组生物中生产化合物
公开(公告)号：US20090233287A1
公开(公告)日：2009-09-17
申请号：US12298191
申请日：2007-04-25
申请人： Roelof Ary Lans Bovenberg , Marco Alexander Van den Berg , Susanne Hage , Paul Klaassen , Bernard Meijrink , Lourina Madeleine Raamsdonk
发明人： Roelof Ary Lans Bovenberg , Marco Alexander Van den Berg , Susanne Hage , Paul Klaassen , Bernard Meijrink , Lourina Madeleine Raamsdonk
IPC分类号： C12Q1/68 , C12N1/19 , C12P1/00 , C12P17/06 , C12P17/04 , C12P7/02 , C12P17/16 , C12P7/42
CPC分类号： C12N9/1029 , C12N9/0004 , C12N9/93 , C12P7/02 , C12P13/02 , C12P17/06 , C12P17/181 , C12P17/182 , C12P17/188
摘要： The present invention provides a recombinant Penicillium chrysogenum strain characterized in that a gene selected from the list consisting of penDE, pcbAB and pcbC is inactivated and the use of such a strain for the preparation of a compound of interest. Furthermore, it is an object of the present invention to provide a method for the production of a compound of interest in a eukaryotic recombinant microorganism comprising the steps of: (a) Reducing the level of secondary metabolite production in said microorganism with 50-100%; (b) Introducing a heterologous gene into said microorganism
摘要翻译：本发明提供重组产黄青霉菌株，其特征在于，从PenDE，pcbAB和pcbC列表中选出的基因被灭活，并且使用这样的菌株来制备感兴趣的化合物。此外，本发明的目的是提供一种在真核重组微生物中生产感兴趣的化合物的方法，包括以下步骤：（a）降低所述微生物中次级代谢产物的产生水平，其中50-100％ ; （b）将异源基因引入所述微生物

3. 发明申请

US20100248233A1 ACETYL-COA PRODUCING ENZYMES IN YEAST 审中-公开
标题翻译：在乙烯生产酶的乙酰辅酶A
公开(公告)号：US20100248233A1
公开(公告)日：2010-09-30
申请号：US12670050
申请日：2008-07-11
申请人： Ulrike Maria Müller , Liang Wu , Lourina Madeleine Raamsdonk , Aaron Adriaan Winkler
发明人： Ulrike Maria Müller , Liang Wu , Lourina Madeleine Raamsdonk , Aaron Adriaan Winkler
IPC分类号： C12Q1/68 , C12N15/63 , C12N1/19 , C12P7/16
CPC分类号： C12Q1/32 , C07K14/33 , C12N9/001 , C12P7/16 , Y02E50/10
摘要： The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.
摘要翻译：本发明涉及一种鉴定具有用于将丙酮酸，乙醛或乙酸酯转化成酵母细胞的（细胞溶质）中的乙酰辅酶A的酶活性的异源多肽的方法，包括：a）提供包含至少一个缺失的突变的酵母细胞选自编码丙酮酸脱羧酶（PDC），乙醛脱氢酶（ALD）和乙酰辅酶A合成酶（ACS）的基因的（PDH）旁路的一个基因; b）用包含异源核苷酸序列的表达载体转化所述突变的酵母细胞，所述异源核苷酸序列编码具有将丙酮酸，乙醛或乙酸转化为乙酰辅酶A的潜在酶活性的候选多肽; c）测试所述重组突变酵母细胞在含有葡萄糖作为唯一碳源的基本培养基上生长的能力，以及d）将所述候选多肽鉴定为异源多肽，其具有将丙酮酸，乙醛或乙酸酯转化成乙酰辅酶A 当所述细胞的生长被观察时，所述酵母细胞的胞质溶胶。本发明还涉及生产发酵生产如丁醇的方法。

4. 发明申请

US20100205857A1 BUTANOL PRODUCTION IN A EUKARYOTIC CELL 审中-公开
标题翻译：丁醇生产在一个欧洲细胞
公开(公告)号：US20100205857A1
公开(公告)日：2010-08-19
申请号：US12669251
申请日：2008-07-11
申请人： Einte-Karst Dijk , Lourina Madeleine Raamsdonk
发明人： Einte-Karst Dijk , Lourina Madeleine Raamsdonk
IPC分类号： C12P7/16 , C12N1/16 , C12N1/19 , C12N15/81 , C07C29/74 , C10L1/182
CPC分类号： C12P7/16 , B01D3/002 , C12P7/06 , C12R1/865 , Y02E50/10 , Y02E50/17
摘要： The present invention relates to a eukaryotic cell capable of producing butanol and ethanol at a ratio butanol:ethanol of between 1:2 to 1:100. The present invention further relates to a process for the preparation of butanol and ethanol comprising fermenting a eukaryotic cell in a suitable fermentation broth, wherein butanol and ethanol are produced at a ratio butanol:hethanol of between 1:2 to 1:100 and a process for the recovery of butanol and ethanol from an aqueous solution comprising butanol and ethanol wherein the ratio butanol:ethanol is between 1:2 to 1:100 comprising, separating an ethanol/butanol/water mixture from the aqueous solution; separating an ethanol/water mixture from the ethanol/butanol/water mixture; separating a butanol/water mixture from the ethanol/butanol/water mixture; and recovering of butanol and ethanol.
摘要翻译：本发明涉及能够以1:2至1:100的丁醇/乙醇比例生产丁醇和乙醇的真核细胞。本发明还涉及一种制备丁醇和乙醇的方法，其包括在合适的发酵液中发酵真核细胞，其中丁醇和乙醇以1:2至1:100的丁醇：乙醇的比例生产，用于从包含丁醇和乙醇的水溶液中回收丁醇和乙醇，其中丁醇：乙醇的比例为1:2至1:100，包括从水溶液中分离出乙醇/丁醇/水混合物; 从乙醇/丁醇/水混合物中分离乙醇/水混合物; 从乙醇/丁醇/水混合物中分离出丁醇/水混合物; 并回收丁醇和乙醇。

5. 发明申请

US20130030146A1 PREPARATION OF CAPROLACTAM FROM 6-AMINO CAPROIC ACID OBTAINED IN A FERMENTATION PROCESS 审中-公开
标题翻译：在发酵过程中获得的6-AMINO大豆酸捕获剂的制备
公开(公告)号：US20130030146A1
公开(公告)日：2013-01-31
申请号：US13518647
申请日：2010-12-22
申请人： Rudolf Philippus Maria Guit , Thomas Van Der Does , Lourina Madeleine Raamsdonk
发明人： Rudolf Philippus Maria Guit , Thomas Van Der Does , Lourina Madeleine Raamsdonk
IPC分类号： C07D223/10 , C08G69/04
CPC分类号： C07D201/08 , C07D201/16 , C07D223/10 , C08G69/16
摘要： The invention relates to a method for preparing caprolactam comprising recovering a mixture containing 6-aminocaproic acid, from a culture medium comprising biomass, and thereafter cyclising the 6-aminocaproic acid in the presence of superheated steam, thereby forming caprolactam, wherein the weight to weight ratio carbohydrate to 6-aminocaproic acid in said mixture is 0.03 or less.
摘要翻译：本发明涉及一种制备己内酰胺的方法，包括从含有生物质的培养基中回收含有6-氨基己酸的混合物，然后在过热蒸汽存在下环化6-氨基己酸，从而形成己内酰胺，其中重量所述混合物中碳水化合物与6-氨基己酸的比例为0.03以下。

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