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1. 发明申请

US20050052630A1 Constant current electroporation device and methods of use 有权
标题翻译：恒流电穿孔装置及其使用方法
公开(公告)号：US20050052630A1
公开(公告)日：2005-03-10
申请号：US10657725
申请日：2003-09-08
申请人： Louis Smith , Ruxandra Draghia-Akli , Amir Khan , Robert Carpenter , Jeff Darnell
发明人： Louis Smith , Ruxandra Draghia-Akli , Amir Khan , Robert Carpenter , Jeff Darnell
IPC分类号： A61N1/30 , A61N1/32 , G03B27/42
CPC分类号： A61N1/0412 , A61N1/0476 , A61N1/327
摘要： An electroporation device which may be used to effectively facilitate the introduction of a macromolecule into cells of a selected tissue in a body or plant. The electroporation device comprises an electro-kinetic device (“EKD”) whose operation is specified by software or firmware. The EKD produces a series of programmable constant-current pulse patterns between electrodes in an array based on user control and input of the pulse parameters and allows the storage and acquisition of current waveform data. The electroporation device also comprises a replaceable electrode disk having an array of needle electrodes, a central injection channel for an injection needle, and a removable guide disk.
摘要翻译：可用于有效促进将大分子引入身体或植物中选定组织的细胞中的电穿孔装置。电穿孔装置包括其动作由软件或固件指定的电动装置（“EKD”）。 EKD基于用户控制和输入脉冲参数，在阵列中的电极之间产生一系列可编程恒定电流脉冲模式，并允许存储和采集电流波形数据。电穿孔装置还包括具有针电极阵列的可替换电极盘，用于注射针的中心注射通道和可移除引导盘。

2. 发明授权

US08209006B2 Constant current electroporation device and methods of use 有权
标题翻译：恒流电穿孔装置及其使用方法
公开(公告)号：US08209006B2
公开(公告)日：2012-06-26
申请号：US10657725
申请日：2003-09-08
申请人： Louis C. Smith , Ruxandra Draghia-Akli , Amir S. Khan , Robert H. Carpenter , Jeff Darnell
发明人： Louis C. Smith , Ruxandra Draghia-Akli , Amir S. Khan , Robert H. Carpenter , Jeff Darnell
IPC分类号： A61N1/30
CPC分类号： A61N1/0412 , A61N1/0476 , A61N1/327
摘要： An electroporation device which may be used to effectively facilitate the introduction of a macromolecule into cells of a selected tissue in a body or plant. The electroporation device comprises an electro-kinetic device (“EKD”) whose operation is specified by software or firmware. The EKD produces a series of programmable constant-current pulse patterns between electrodes in an array based on user control and input of the pulse parameters and allows the storage and acquisition of current waveform data. The electroporation device also comprises a replaceable electrode disk having an array of needle electrodes, a central injection channel for an injection needle, and a removable guide disk.
摘要翻译：可用于有效促进将大分子引入身体或植物中选定组织的细胞中的电穿孔装置。电穿孔装置包括其动作由软件或固件指定的电动装置（“EKD”）。 EKD基于用户控制和输入脉冲参数，在阵列中的电极之间产生一系列可编程恒定电流脉冲模式，并允许存储和采集电流波形数据。电穿孔装置还包括具有针电极阵列的可替换电极盘，用于注射针的中心注射通道和可移除引导盘。

3. 发明授权

US07238522B2 Devices and methods for biomaterial production 有权
标题翻译：生物材料生产的设备和方法
公开(公告)号：US07238522B2
公开(公告)日：2007-07-03
申请号：US10857439
申请日：2004-05-27
申请人： Henry Hebel , Sriram Ramakrishnan , Hugo Gonzalez , Jeff Darnell
发明人： Henry Hebel , Sriram Ramakrishnan , Hugo Gonzalez , Jeff Darnell
IPC分类号： C12M1/33
CPC分类号： C12N15/1003 , B01F13/0255 , B03D2203/003 , C12M47/06 , C12M47/10 , C12N1/06 , C12N1/066 , C12N15/101
摘要： An apparatus and a method for isolating a biologic product, such as plasmid DNA, from cells. The method involves lysing cells in a controlled manner separate insoluble components from a fluid lysate containing cellular components of interest, followed by membrane chromatographic techniques to purify the cellular components of interest. The process utilizes a unique lysis apparatus, ion exchange and, optionally, hydrophobic interaction chromatography membranes in cartridge form, and ultrafiltration. The process can be applied to any biologic product extracted from a cellular source. The process uses a lysis apparatus, including a high shear, low residence-time mixer for advantageously mixing a cell suspension with a lysis solution, a hold time that denatures impurities, and an air-sparging bubble mixer that gently yet thoroughly mixes lysed cells with a neutralization/precipitation buffer and floats compacted precipitated cellular material.
摘要翻译：从细胞中分离生物制品如质粒DNA的装置和方法。该方法包括以受控的方式裂解细胞，从含有感兴趣的细胞成分的流体裂解物中分离不溶性组分，然后用膜色谱技术纯化感兴趣的细胞组分。该方法利用独特的裂解装置，离子交换以及任选的以胶囊形式的疏水相互作用层析膜和超滤。该方法可以应用于从细胞来源提取的任何生物制品。该方法使用裂解装置，包括高剪切，低停留时间混合器，有利地将细胞悬浮液与裂解溶液混合，使杂质变性的保持时间和将裂解细胞轻轻地充分混合的空气鼓泡混合器中和/沉淀缓冲液和漂浮压实的沉淀细胞材料。

4. 发明申请

US20050014245A1 Devices and methods for biomaterial production 有权
标题翻译：生物材料生产的设备和方法
公开(公告)号：US20050014245A1
公开(公告)日：2005-01-20
申请号：US10857439
申请日：2004-05-27
申请人： Henry Hebel , Sriram Ramakrishnan , Hugo Gonzalez , Jeff Darnell
发明人： Henry Hebel , Sriram Ramakrishnan , Hugo Gonzalez , Jeff Darnell
IPC分类号： B01F13/02 , C12N1/06 , C12N15/10 , C12P21/06 , C12M1/34 , C12N1/08
CPC分类号： C12N15/1003 , B01F13/0255 , B03D2203/003 , C12M47/06 , C12M47/10 , C12N1/06 , C12N1/066 , C12N15/101
摘要： An apparatus and a method for isolating a biologic product, such as plasmid DNA, from cells. The method involves lysing cells in a controlled manner separate insoluble components from a fluid lysate containing cellular components of interest, followed by membrane chromatographic techniques to purify the cellular components of interest. The process utilizes a unique lysis apparatus, ion exchange and, optionally, hydrophobic interaction chromatography membranes in cartridge form, and ultrafiltration. The process can be applied to any biologic product extracted from a cellular source. The process uses a lysis apparatus, including a high shear, low residence-time mixer for advantageously mixing a cell suspension with a lysis solution, a hold time that denatures impurities, and an air-sparging bubble mixer that gently yet thoroughly mixes lysed cells with a neutralization/precipitation buffer and floats compacted precipitated cellular material.
摘要翻译：从细胞中分离生物制品如质粒DNA的装置和方法。该方法包括以受控的方式裂解细胞，从含有感兴趣的细胞成分的流体裂解物中分离不溶性组分，然后用膜色谱技术纯化感兴趣的细胞组分。该方法利用独特的裂解装置，离子交换以及任选的以胶囊形式的疏水相互作用层析膜和超滤。该方法可以应用于从细胞来源提取的任何生物制品。该方法使用裂解装置，包括高剪切，低停留时间混合器，有利地将细胞悬浮液与裂解溶液混合，使杂质变性的保持时间和将裂解细胞轻轻地充分混合的空气鼓泡混合器中和/沉淀缓冲液和漂浮压实的沉淀细胞材料。

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