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1. 发明授权

US08715966B2 Preparation of templates for nucleic acid sequencing 有权
标题翻译：核酸测序模板的制备
公开(公告)号：US08715966B2
公开(公告)日：2014-05-06
申请号：US13450920
申请日：2012-04-19
申请人： Liu Xiaohai , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Liu Xiaohai , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC分类号： C12Q1/68 , C12P19/34 , C40B40/06
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
摘要翻译：本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上用于核酸测序反应的单链模板。

2. 发明申请

US20140349891A1 Preparation of Templates for Nucleic Acid Sequencing 有权
标题翻译：核酸测序模板的制备
公开(公告)号：US20140349891A1
公开(公告)日：2014-11-27
申请号：US14166670
申请日：2014-01-28
申请人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC分类号： C12Q1/68
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction,
摘要翻译：本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上用于核酸测序反应的单链模板，

3. 发明授权

US09085802B2 Preparation of templates for nucleic acid sequencing 有权
标题翻译：核酸测序模板的制备
公开(公告)号：US09085802B2
公开(公告)日：2015-07-21
申请号：US14166670
申请日：2014-01-28
申请人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC分类号： C12Q1/68
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
摘要翻译：本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上用于核酸测序反应的单链模板。

4. 发明申请

US20090118128A1 Preparation of templates for nucleic acid sequencing 审中-公开
标题翻译：核酸测序模板的制备
公开(公告)号：US20090118128A1
公开(公告)日：2009-05-07
申请号：US11989169
申请日：2006-07-20
申请人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC分类号： C40B20/00 , C07H1/00 , C12P19/34 , C40B50/06
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
摘要翻译：本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上用于核酸测序反应的单链模板。

5. 发明申请

US20120208194A1 Preparation of Templates for Nucleic Acid Sequencing 有权
标题翻译：核酸测序模板的制备
公开(公告)号：US20120208194A1
公开(公告)日：2012-08-16
申请号：US13450920
申请日：2012-04-19
申请人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction,
摘要翻译：本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上用于核酸测序反应的单链模板，

6. 发明授权

US08945835B2 Method for sequencing a polynucleotide template 有权
标题翻译：多核苷酸模板测序方法
公开(公告)号：US08945835B2
公开(公告)日：2015-02-03
申请号：US13444654
申请日：2012-04-11
申请人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
发明人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要： The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
摘要翻译：本发明提供了双链多核苷酸模板的成对测序的方法，该方法导致多核苷酸模板的两个不同和分离的区域中的核苷酸序列的顺序测定。

7. 发明申请

US20130165327A1 Method for Sequencing a Polynucelotide Template 有权
公开(公告)号：US20130165327A1
公开(公告)日：2013-06-27
申请号：US13444654
申请日：2012-04-11
申请人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
发明人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要： The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.

8. 发明授权

US08192930B2 Method for sequencing a polynucleotide template 有权
标题翻译：多核苷酸模板测序方法
公开(公告)号：US08192930B2
公开(公告)日：2012-06-05
申请号：US12223759
申请日：2007-02-08
申请人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
发明人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要： The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
摘要翻译：本发明提供了双链多核苷酸模板的成对测序的方法，该方法导致多核苷酸模板的两个不同和分离的区域中的核苷酸序列的顺序测定。

9. 发明申请

US20110009276A1 Method for Sequencing a Polynucleotide Template 有权
标题翻译：多核苷酸模板测序方法
公开(公告)号：US20110009276A1
公开(公告)日：2011-01-13
申请号：US12223759
申请日：2007-02-08
申请人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias Willaim Barr Ost , David Bentley
发明人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias Willaim Barr Ost , David Bentley
IPC分类号： C40B30/00
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要： The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
摘要翻译：本发明提供了双链多核苷酸模板的成对测序的方法，该方法导致多核苷酸模板的两个不同和分离的区域中的核苷酸序列的顺序测定。

10. 发明申请

US20100167283A1 Analyte Detection 审中-公开
标题翻译：分析物检测
公开(公告)号：US20100167283A1
公开(公告)日：2010-07-01
申请号：US12438159
申请日：2007-09-06
申请人： Adrian Martin Horgan , Graham John Worsley
发明人： Adrian Martin Horgan , Graham John Worsley
IPC分类号： C12Q1/68
CPC分类号： G01N33/54373 , C12Q1/6825 , G01N33/58
摘要： A method of detecting an analyte in a sample, comprises the steps of: a) contacting the sample with a first ligand which binds specifically to the analyte and which is immobilised either on, or in the vicinity of, a sensor; b) prior to step (a) contacting the sample, or subsequent to step (a) contacting the immobilised analyte, with a material including a second ligand which binds specifically to the analyte, the material being activatable to form a polymerisation initiator; and c) activating the material; wherein the polymerisation initiator interacts with the sensor to change its physical properties, which causes a change in the optical or acoustic properties of the sensor.
摘要翻译：检测样品中分析物的方法包括以下步骤：a）使样品与特异性结合分析物结合并固定在传感器上或其附近的第一配体接触; b）在步骤（a）之前或之后步骤（a）使固定化分析物接触的材料与包括特异性结合分析物的第二配体的材料接触，所述材料可活化以形成聚合引发剂; 和c）激活材料; 其中所述聚合引发剂与所述传感器相互作用以改变其物理性质，这导致所述传感器的光学或声学特性的变化。

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