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1. 发明申请

US20090239758A1 METHODS, MICROARRAY, AND KITS FOR DETECTION OF DRUG RESISTANCE GENES IN GRAM-NEGATIVE BACTERIA 有权
标题翻译：用于检测抗菌细菌中耐药基因的方法，微阵列和试剂盒
公开(公告)号：US20090239758A1
公开(公告)日：2009-09-24
申请号：US12097237
申请日：2007-01-18
申请人： Lingxiang Zhu , Zhiwei Zhang , Di Jiang , Ning Du , Can Wang , Huawei Yang , Qiong Zhang , Huafang Gao , Yuxiang Zhou , Jing Cheng
发明人： Lingxiang Zhu , Zhiwei Zhang , Di Jiang , Ning Du , Can Wang , Huawei Yang , Qiong Zhang , Huafang Gao , Yuxiang Zhou , Jing Cheng
IPC分类号： C40B30/04 , C40B40/08 , C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/156 , C12Q2600/16 , C12Q2600/166
摘要： The present invention provides kits and microarrays containing primer pairs for amplifying drug resistance genes and/or probes for detection of drug resistance genes. Also provided are methods of detecting drug resistance genes using kits and microarrays described herein.
摘要翻译：本发明提供了包含用于扩增用于检测药物抗性基因的药物抗性基因和/或探针的引物对的试剂盒和微阵列。还提供了使用本文所述的试剂盒和微阵列检测药物抗性基因的方法。

2. 发明授权

US08124382B2 Methods, microarray, and kits for detection of drug resistance genes in gram-negative bacteria 有权
标题翻译：用于检测革兰氏阴性菌耐药基因的方法，微阵列和试剂盒
公开(公告)号：US08124382B2
公开(公告)日：2012-02-28
申请号：US12097237
申请日：2007-01-18
申请人： Lingxiang Zhu , Zhiwei Zhang , Di Jiang , Ning Du , Can Wang , Huawei Yang , Qiong Zhang , Huafang Gao , Yuxiang Zhou , Jing Cheng
发明人： Lingxiang Zhu , Zhiwei Zhang , Di Jiang , Ning Du , Can Wang , Huawei Yang , Qiong Zhang , Huafang Gao , Yuxiang Zhou , Jing Cheng
IPC分类号： C12P19/34
CPC分类号： C12Q1/689 , C12Q2600/156 , C12Q2600/16 , C12Q2600/166
摘要： The present invention provides kits and microarrays containing primer pairs for amplifying drug resistance genes and/or probes for detection of drug resistance genes. Also provided are methods of detecting drug resistance genes using kits and microarrays described herein.
摘要翻译：本发明提供了包含用于扩增用于检测药物抗性基因的药物抗性基因和/或探针的引物对的试剂盒和微阵列。还提供了使用本文所述的试剂盒和微阵列检测药物抗性基因的方法。

3. 发明授权

US08735067B2 Asymmetric PCR amplification, its special primer and application 有权
标题翻译：不对称PCR扩增，其特殊引物及应用
公开(公告)号：US08735067B2
公开(公告)日：2014-05-27
申请号：US11661069
申请日：2004-11-22
申请人： Zhiwei Zhang , Can Wang , Lingxiang Zhu , Qiong Zhang , Jing Cheng
发明人： Zhiwei Zhang , Can Wang , Lingxiang Zhu , Qiong Zhang , Jing Cheng
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , C12Q2531/107 , C12Q2525/155 , C12Q2525/15
摘要： The present invention discloses an asymmetric PCR amplification method, its special primer and application, aims to provide a simple, effective PCR amplification for preparation of single-stranded product. The asymmetric PCR primer of the invention comprises some PCR primer pairs, in which an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one primer. The asymmetric PCR amplification provided includes the steps: 1) preparative denaturing; 2) repetitiously denaturing, primers annealing, extending cycles as the first stage of PCR amplification; 3) repetitiously denaturing, primer extending cycles as the second stage of PCR amplification, wherein an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one PCR primer of each pair in extension. With the asymmetric PCR amplification of the invention, high throughput of single-stranded products can be obtained, single PCR amplification or multiple PCR amplification can be carried out. And the method can be widely used in detection of nucleic acids.
摘要翻译：本发明公开了一种不对称PCR扩增方法及其特异性引物及其应用，旨在为单链产物的制备提供简单有效的PCR扩增。本发明的不对称PCR引物包含一些PCR引物对，其中将待检测的靶序列的不相关核酸序列添加到一个引物的5'末端。提供的不对称PCR扩增包括以下步骤：1）制备型变性; 2）重复变性，引物退火，延伸循环作为PCR扩增的第一阶段; 3）重复变性，引物延伸循环作为PCR扩增的第二阶段，其中将待检测的靶序列的不相关核酸序列添加到每对扩增中的一个PCR引物的5'末端。利用本发明的不对称PCR扩增，可以获得高通量的单链产物，可进行单PCR扩增或多重PCR扩增。该方法可广泛用于检测核酸。

4. 发明申请

US20100151448A1 Asymmetric PCR Amplification, its Special Primer and Application 有权
标题翻译：不对称PCR扩增，其特殊引物和应用
公开(公告)号：US20100151448A1
公开(公告)日：2010-06-17
申请号：US11661069
申请日：2004-11-22
申请人： Zhiwei Zhang , Can Wang , Lingxiang Zhu , Qiong Zhang , Jing Cheng
发明人： Zhiwei Zhang , Can Wang , Lingxiang Zhu , Qiong Zhang , Jing Cheng
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/686 , C12Q2531/107 , C12Q2525/155 , C12Q2525/15
摘要： The present invention discloses an asymmetric PCR amplification method, its special primer and application, aims to provide a simple, effective PCR amplification for preparation of single-stranded product. The asymmetric PCR primer of the invention comprises some PCR primer pairs, in which an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one primer. The asymmetric PCR amplification provided includes the steps: 1) preparative denaturing; 2) repetitiously denaturing, primers annealing, extending cycles as the first stage of PCR amplification; 3) repetitiously denaturing, primer extending cycles as the second stage of PCR amplification, wherein an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one PCR primer of each pair in extension. With the asymmetric PCR amplification of the invention, high throughput of single-stranded products can be obtained, single PCR amplification or multiple PCR amplification can be carried out. And the method can be widely used in detection of nucleic acids.
摘要翻译：本发明公开了一种不对称PCR扩增方法及其特异性引物及其应用，旨在为单链产物的制备提供简单有效的PCR扩增。本发明的不对称PCR引物包含一些PCR引物对，其中将待检测的靶序列的不相关核酸序列添加到一个引物的5'末端。提供的不对称PCR扩增包括以下步骤：1）制备型变性; 2）重复变性，引物退火，延伸循环作为PCR扩增的第一阶段; 3）重复变性，引物延伸循环作为PCR扩增的第二阶段，其中将待检测的靶序列的不相关核酸序列添加到每对扩增中的一个PCR引物的5'末端。利用本发明的不对称PCR扩增，可以获得高通量的单链产物，可进行单PCR扩增或多重PCR扩增。该方法可广泛用于检测核酸。

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