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1. 发明授权

US06703541B1 Nematode-upregulated peroxidase gene promoter from nematode-resistant maize line Mp307 有权
标题翻译：来自线虫抗性玉米品系Mp307的线虫上调的过氧化物酶基因启动子
公开(公告)号：US06703541B1
公开(公告)日：2004-03-09
申请号：US09716410
申请日：2000-11-21
申请人： Linas S. Padegimas , Nancy A. Reichert
发明人： Linas S. Padegimas , Nancy A. Reichert
IPC分类号： C12N1509
CPC分类号： C12N9/0065 , C12N9/0004 , C12N15/8237 , C12N15/8239 , C12N15/8285 , Y02A40/164
摘要： The present invention provides a nematode inducible promoter sequence from maize inbred line Mp307, and DNA constructs and transcription cassettes comprising said promoter sequence, a gene encoding a toxin, and a termination sequence. Methods for inducing nematode resistance in plants and transgenic plants resistant to nematode infection are also provided.
摘要翻译：本发明提供了来自玉米近交系Mp307的线虫诱导型启动子序列，以及包含所述启动子序列，编码毒素的基因和终止序列的DNA构建体和转录盒。还提供了在植物中诱导线虫抗性的方法和对线虫感染有抗性的转基因植物。

2. 发明授权

US6140555A Methods for maize transformation coupled with adventitious regeneration utilizing nodal section explants and mature zygotic embryos 失效
标题翻译：玉米转化方法与利用节段外植体和成熟合子胚胎的不定期再生相结合
公开(公告)号：US6140555A
公开(公告)日：2000-10-31
申请号：US92180
申请日：1998-06-05
申请人： Nancy A. Reichert , Vanishree Rudraswamy
发明人： Nancy A. Reichert , Vanishree Rudraswamy
IPC分类号： C12N15/82 , A01H4/00 , A01H1/00 , C12N5/04 , C12N15/87
CPC分类号： C12N15/8207
摘要： Maize tissue may be regenerated from nodal extracts prepared from germinated mature seeds and germinated embryos. Nodal section explants are secured from seedlings in 3-5 days. The explants are grown on an induction medium until adventitious shoot formation is observed. The shoots are separated and elongated on an MS-based medium, and then rooted. Fast genotype-independent regeneration is obtained, in 12-14 weeks. These explants, as well as zygotic embryos, may be transformed with exogenous DNA using a biolistic approach, where DNA precipitated onto tungsten microprojectiles is accelerated as 650 psi towards the explants at a distance of at least 7.5 microns. Improved frequency of transformation is obtained using microprojectiles which prior to DNA precipitation were frozen in glycerol, and suspending from a preparation of 2.5 M CaCl.sub.2. The combination of transformation process and regeneration can be used, independent of genotype, to provide new commercial crop organisms.
摘要翻译：可以从由发芽的成熟种子和发芽胚中制备的节点提取物中再生玉米组织。结节外植体在3-5天内从幼苗中获得。外植体在诱导培养基上生长，直到观察到不定芽形成。芽在MS基培养基上分离和细长，然后扎根。在12-14周内获得快速基因型独立再生。这些外植体以及合子胚可以使用生物弹性方法用外源DNA转化，其中沉淀到钨微粒上的DNA以至少7.5微米的距离向外植体加速650psi。使用在DNA沉淀之前的微粒子在甘油中冷冻并从2.5M CaCl 2的制剂中悬浮获得改进的转化频率。可以使用转基因和再生的组合，与基因型无关，提供新的商业作物生物。

3. 发明授权

US06479287B1 Method for transformation of cotton and organogenic regeneration 有权
标题翻译：棉花和器官再生转化方法
公开(公告)号：US06479287B1
公开(公告)日：2002-11-12
申请号：US09562193
申请日：2000-05-01
申请人： Nancy A. Reichert , Teong-Kwee Lim , Margaret M. Young
发明人： Nancy A. Reichert , Teong-Kwee Lim , Margaret M. Young
IPC分类号： C12N502
CPC分类号： C12N15/8201 , A01H4/001 , A01H4/005 , A01H4/008 , C12N5/04
摘要： Protocols for organogenic regeneration of cotton are provided, which makes the in vitro regeneration of mature fertile plants in a reduced amount of time possible. Seedlings are the basis for monocotyl or hypocotyl explants which are transferred from the germination medium to a shoot initiation medium which comprises AgNO3. These explants, prior to shoot initiation, may be transformed with exogenous DNA, either through inoculation with an Agorbacterium agent such as A. tumefaciens, or through biolistic bombardment of the explants with microprojectiles having the exogenous DNA adsorbed onto their surface.
摘要翻译：提供棉花有机生殖再生方案，使成熟可育植物的体外再生成为可能。幼苗是从发芽培养基转移到包含AgNO3的芽起始培养基的单子叶植物或下胚轴外植体的基础。这些外植体在芽起始之前可以用外源DNA转化，或者通过用Agorbacterium试剂如根癌土壤杆菌接种，或者通过对具有吸附在其表面上的外源DNA的微粒子的外植体进行生物射击轰击。

4. 发明授权

US06357176B2 Soilless sod 失效
标题翻译：无土豆
公开(公告)号：US06357176B2
公开(公告)日：2002-03-19
申请号：US08825550
申请日：1997-03-19
申请人： Brian S. Baldwin , J. Michael Goatley, Jr. , Marty J. Fuller , Nancy A. Reichert , Kevin L. Hensler
发明人： Brian S. Baldwin , J. Michael Goatley, Jr. , Marty J. Fuller , Nancy A. Reichert , Kevin L. Hensler
IPC分类号： A01C104
CPC分类号： A01C1/046 , A01C1/044 , A01G24/00 , Y02P60/216
摘要： A soil- and grass seed-less sod precursor containing a non-woven bio-cellulosic fiber mat and grass sprigs. The sod precursor can be used to produce a soil-free sod which is useful for manufacturing athletic fields, golf courses and lawns.
摘要翻译：含有无纺布生物纤维素纤维垫和草小枝的无土壤和无草种子的母体前体。草原前体可以用于生产无土壤的草皮，可用于制造运动场，高尔夫球场和草坪。

5. 发明授权

US6153813A Methods for genotype-independent nuclear and plastid transformation coupled with clonal regeneration utilizing mature zygotic embryos in rice (Oryza sativa) seeds 失效
标题翻译：基因型无关核和质体转化的方法与利用水稻（Oryza sativa）种子中成熟合子胚的克隆再生相结合
公开(公告)号：US6153813A
公开(公告)日：2000-11-28
申请号：US209466
申请日：1998-12-11
申请人： Nancy A. Reichert , Vanishree Rudraswamy , Liza Ming-Ju Chen
发明人： Nancy A. Reichert , Vanishree Rudraswamy , Liza Ming-Ju Chen
IPC分类号： C12N15/82 , A07H1/00 , A01H1/06 , C12N15/00 , C12N15/29
CPC分类号： C12N15/8207 , C12N15/8214
摘要： A method for transforming rice plants to express heterologous DNA which involves the biolistic bombardment of mature rice seeds (embryo bearing) and results in expression of the heterologous DNA coated on the biolistic particle in the post-bombardment embryo. The heterologous DNA may be introduced as a plastid, as opposed to bare DNA. The transformed rice embryo is regenerated into a rice plantlet which can be transferred from greenhouse to field.
摘要翻译：一种用于转化水稻植物以表达异源DNA的方法，其涉及成熟水稻种子（携带胚胎）的生物射弹轰击，并导致在后轰击胚中涂覆在生物弹性粒子上的异源DNA的表达。异源DNA可以作为质体引入，而不是裸DNA。将转化的水稻胚胎再生成可以从温室转移到田间的水稻苗。

6. 发明授权

US06570068B1 Methods for maize transformation coupled with adventitious regeneration utilizing nodal section explants and mature zygotic embryos 有权
标题翻译：玉米转化方法与利用节段外植体和成熟合子胚胎的不定期再生相结合
公开(公告)号：US06570068B1
公开(公告)日：2003-05-27
申请号：US09698080
申请日：2000-10-30
申请人： Margaret M. Young , Nancy A. Reichert
发明人： Margaret M. Young , Nancy A. Reichert
IPC分类号： A01H100
CPC分类号： C12N15/8207
摘要： Maize tissue may be regenerated from nodal extracts prepared from germinated mature seeds and germinated embryos grown in a medium containing plant growth regulators. Nodal section explants are secured from seedlings approximately 3-10 days old, preferably from 3-7 days old. The explants are grown on an induction medium until adventitious shoot formation is observed. The shoots are separated and elongated on an MS-based medium, and then rooted. Fast genotype-independent regeneration is obtained, in 12-14 weeks. These explants, as well as zygotic embryos, may be transformed with exogenous DNA using a biolistic approach, where DNA precipitated onto tungsten microprojectiles is accelerated at a minimum of 650 psi towards the explants at a distance of at least 7.5 cm. Improved frequency of transformation is obtained using microprojectiles which prior to DNA precipitation were frozen in glycerol, and suspending from a preparation of 2.5 M CaCl2. The combination of transformation process and regeneration can be used, independent of genotype, to provide new commercial crop organisms.
摘要翻译：可以从由发芽成熟种子制备的节点提取物和在含有植物生长调节剂的培养基中生长的发芽胚胎再生玉米组织。从约3-10天龄的幼苗中获得节段外植体，优选3-7天龄。外植体在诱导培养基上生长，直到观察到不定芽形成。芽在MS基培养基上分离和细长，然后扎根。在12-14周内获得快速基因型独立再生。这些外植体以及合子胚可以使用生物弹道学方法用外源DNA转化，其中沉淀到钨微粒上的DNA在至少7.5cm的距离处以至少650psi的速度向外植体加速。使用在DNA沉淀之前的微粒子在甘油中冷冻并从2.5M CaCl 2的制剂中悬浮获得改进的转化频率。可以使用转基因和再生的组合，与基因型无关，提供新的商业作物生物。

7. 发明授权

US5998207A Method for transformation of cotton and kenaf and organogenic regeneration 失效
标题翻译：棉花和洋麻的转化方法和器官再生
公开(公告)号：US5998207A
公开(公告)日：1999-12-07
申请号：US96360
申请日：1998-06-12
申请人： Nancy A. Reichert , Teong-Kwee Lim , Margaret M. Young
发明人： Nancy A. Reichert , Teong-Kwee Lim , Margaret M. Young
IPC分类号： A01H4/00 , C12N15/82 , A01H1/00 , C12N15/84
CPC分类号： C12N15/8207 , A01H4/008 , C12N15/8205
摘要： Protocols for ogranogenic regeneration of cotton and kenaf are provided, which makes the in vitro regeneration of mature fertile plants in a reduced amount of time possible. Seedlings are the basis for monocotyl or hypocotyl explants which are transferred from the germination medium to a shoot initiation medium which comprises AgNO.sub.3. These explants, prior to shoot initiation, may be transformed with exogenous DNA either through inoculation with a Agrobacterium agent such as A. tumefaciens, or through biolistic bombardment of the explants with microprojectiles having the exogenous DNA adsorbed onto their surface.
摘要翻译：提供了棉花和洋麻的无机物再生方案，这使得成熟可育植物的体外再生成为可能的时间。幼苗是从发芽培养基转移到包含AgNO3的芽起始培养基的单子叶植物或下胚轴外植体的基础。这些外植体在芽起始之前可以通过用农杆菌剂如根癌土壤杆菌接种或通过用具有吸附在其表面上的外源DNA的微粒子的外植体的生物射击轰击外源DNA转化。

8. 发明授权

US5968830A Soybean transformation and regeneration methods 失效
标题翻译：大豆转化和再生方法
公开(公告)号：US5968830A
公开(公告)日：1999-10-19
申请号：US825469
申请日：1997-03-28
申请人： Yinghui Dan , Nancy A. Reichert
发明人： Yinghui Dan , Nancy A. Reichert
IPC分类号： A01H4/00 , C12N15/82 , C12N5/10 , A01H5/00
CPC分类号： A01H4/005 , C12N15/8201 , C12N15/8205 , C12N15/8207
摘要： A method of transforming and regenerating soybean plants relies on selection of hypocotyl explants as the target material. Hypocotyl explants can be transformed either by microparticle bombardment with DNA-coated microparticles of inert metals, or by co-culturing with an Agrobacterium strain. The transformed explants can be successfully regenerated, using a protocol including culturing on a shoot induction medium, followed by transfer to a shoot elongation medium to form rooted plantlets, which are transplanted to soil.
摘要翻译：大豆植物转化和再生的方法依赖于选择下胚轴外植体作为靶材料。下胚轴外植体可以通过微粒轰击与DNA包被的惰性金属微粒或通过与农杆菌菌株共培养来转化。可以使用包括在芽诱导培养基上培养的方案成功地再生转化的外植体，然后转移到芽伸长培养基中以形成植入到土壤中的有根的小植株。

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