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1. 发明申请

WO2020104769A1 DEVICE AND METHOD FOR MICRODROPLET DETECTION OF CELLS 审中-公开
公开(公告)号：WO2020104769A1
公开(公告)日：2020-05-28
申请号：PCT/GB2019/053168
申请日：2019-11-08
申请人： LIGHTCAST DISCOVERY LTD
发明人： ISAAC, Tom , FRAYLING, Cameron
IPC分类号： B01L3/00 , G01N35/08 , G01N33/48
摘要： Devices, systems, and associated methods are provided for manipulating and/or determining one or more characteristics of cells contained within a biological sample. In particular a device and methods of use thereof are provided, the device comprising a sorting component configured to separate cell-containing microdroplets from empty ones into a population of cell-containing first microdroplets; a microdroplet manipulation component configured to manipulate the first microdroplets using real or virtual electrowetting electrodes, and an optical detection system configured to detect an optical signal from the microdroplets via the one or more detection windows.

2. 发明申请

WO2020161500A1 MICRODROPLET MANIPULATION METHOD 审中-公开
公开(公告)号：WO2020161500A1
公开(公告)日：2020-08-13
申请号：PCT/GB2020/050280
申请日：2020-02-07
申请人： LIGHTCAST DISCOVERY LTD
发明人： ISAAC, Tom , BALMFORTH, Barnaby , CONTERIO, Jasmin , JOHNSON, Kerr Francis , SOSNA, Maciej , INGHAM, Richard , PODD, Gareth
IPC分类号： B01L3/00
摘要： A method of manipulating microdroplets having an average volume in the range 0.5 femtolitres to 10 nanolitres comprised of at least one biological component and a first aqueous medium having a water activity of a w1 of less than 1 is provided. It is characterised by the step of maintaining the microdroplets in a water-immiscible carrier fluid which further includes secondary droplets having an average volume less than 25% of the average volume of the microdroplets up to and including a maximum of 4 femtolitres and wherein the volume ratio of carrier fluid to total volume of microdroplets per unit volume of the total is greater than 2:1. The method may be employed for example with microdroplets containing biological cells or with microdroplets containing single nucleoside phosphate such as are prepared in a droplet-based nucleic acid sequencer. The method is suitable for controlling for example cellular, chemical or enzymatic processes and/or microdroplet size in microdroplets or single nucleotide nucleic acid sequencing.

3. 发明公开

EP4336168A3 DEVICE AND METHOD FOR MICRODROPLET DETECTION OF CELLS 审中-实审
公开(公告)号：EP4336168A3
公开(公告)日：2024-05-29
申请号：EP24154515.1
申请日：2019-11-08
申请人： Lightcast Discovery Ltd
发明人： ISAAC, Tom , FRAYLING, Cameron
IPC分类号： B01L3/00 , C12M1/00 , C12M1/12 , G01N15/14 , G01N15/10 , G01N15/1492 , G01N33/48
CPC分类号： B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L2200/065220130101 , B01L2300/065420130101 , B01L2300/086420130101 , B01L2300/086720130101 , B01L2400/042720130101 , C12M25/01 , C12M47/04 , G01N15/1484 , G01N15/1456 , G01N2015/148120130101 , G01N2015/10320240101 , G01N15/1492
摘要： A method for using a device for manipulating and/or determining one or more characteristics of cells or micro-bead contained within a biological sample is provided. The device comprises: a sorting component configured to separate cell-containing or micro-bead containing microdroplets from empty ones into a population of cell-containing or micro-bead containing first microdroplets; a microdroplet manipulation component configured to manipulate the first microdroplets in a first zone configured to arrange the first microdroplets into an array for optical inspection and a second zone located within or adjacent the first zone and configured to detect merged microdroplets in one or more detection windows; and an optical detection system configured to detect an optical signal from the microdroplets via the one or more detection windows and to analyse the contents of each microdroplet to determine one or more characteristics of a cell or micro-bead contained in that microdroplet. The method comprises the steps of: creating from the biological sample aqueous first microdroplets in an immiscible carrier fluid, at least some of which are believed to contain cells of a particular cell type; moving the first microdroplets along a pathway using real or virtual electrodes to at least one microdroplet-merging location; merging reporter system containing microdroplets into the first microdroplets to produce merged microdroplets; analyzing the contents of each merged microdroplet with an optical detection system and detecting an optical signal characteristic of an interaction between the cell or micro-bead and the reporter system.

4. 发明公开

EP3883689A1 DEVICE AND METHOD FOR MICRODROPLET DETECTION OF CELLS 有权
公开(公告)号：EP3883689A1
公开(公告)日：2021-09-29
申请号：EP19801405.2
申请日：2019-11-08
申请人： Lightcast Discovery Ltd
发明人： ISAAC, Tom , FRAYLING, Cameron
IPC分类号： B01L3/00 , G01N35/08 , G01N33/48

5. 发明公开

EP4336168A2 DEVICE AND METHOD FOR MICRODROPLET DETECTION OF CELLS 审中-实审
公开(公告)号：EP4336168A2
公开(公告)日：2024-03-13
申请号：EP24154515.1
申请日：2019-11-08
申请人： Lightcast Discovery Ltd
发明人： ISAAC, Tom , FRAYLING, Cameron
IPC分类号： G01N15/14
摘要： A method for using a device for manipulating and/or determining one or more characteristics of cells or micro-bead contained within a biological sample is provided. The device comprises: a sorting component configured to separate cell-containing or micro-bead containing microdroplets from empty ones into a population of cell-containing or micro-bead containing first microdroplets; a microdroplet manipulation component configured to manipulate the first microdroplets in a first zone configured to arrange the first microdroplets into an array for optical inspection and a second zone located within or adjacent the first zone and configured to detect merged microdroplets in one or more detection windows; and an optical detection system configured to detect an optical signal from the microdroplets via the one or more detection windows and to analyse the contents of each microdroplet to determine one or more characteristics of a cell or micro-bead contained in that microdroplet. The method comprises the steps of: creating from the biological sample aqueous first microdroplets in an immiscible carrier fluid, at least some of which are believed to contain cells of a particular cell type; moving the first microdroplets along a pathway using real or virtual electrodes to at least one microdroplet-merging location; merging reporter system containing microdroplets into the first microdroplets to produce merged microdroplets; analyzing the contents of each merged microdroplet with an optical detection system and detecting an optical signal characteristic of an interaction between the cell or micro-bead and the reporter system.

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