专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US6033880A Nucleic acid amplification reaction apparatus and method 失效
标题翻译：核酸扩增反应装置及方法
公开(公告)号：US6033880A
公开(公告)日：2000-03-07
申请号：US27989
申请日：1998-02-23
申请人： Lawrence A. Haff , Enrico Picozza , Will Bloch , Robert Ragusa , Joseph DiCesare , David Tracy , Paul Saviano , Timothy M. Woudenberg , Richard W. Noreiks
发明人： Lawrence A. Haff , Enrico Picozza , Will Bloch , Robert Ragusa , Joseph DiCesare , David Tracy , Paul Saviano , Timothy M. Woudenberg , Richard W. Noreiks
IPC分类号： C12N15/09 , B01J19/00 , B01L7/00 , C12M1/00 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N35/00 , C12P19/34
CPC分类号： B01L7/525 , B01J19/0046 , B01L7/52 , B01L9/065 , B01J2219/00495 , B01J2219/00722 , B01L2300/1883 , C12Q1/686 , C40B40/06 , C40B60/14 , G01N2035/00237 , Y10T436/116664
摘要： Apparatus and method for performing a nucleic acid amplification reaction and preferably a polymerase chain reaction (PCR) in a reaction mixture in at least one capillary tube. Several different embodiments are disclosed. One embodiment cycles a sample through a capillary tube loop passing through two thermostatted fluid baths. Another embodiment has the capillary tube routed alternatingly between two heat exchangers to that the sample makes only one pass through the tube. Other embodiments maintain the heat exchangers stationary and translate the samples between them. Still further embodiments maintain the samples stationary and either automatically translate or rotate the heat exchangers past the samples contained within the capillary tubes to perform the thermal cycles necessary for the amplification reaction.
摘要翻译：用于在至少一个毛细管中的反应混合物中进行核酸扩增反应和优选聚合酶链式反应（PCR）的装置和方法。公开了几个不同的实施例。一个实施例通过穿过两个恒温流体浴的毛细管回路循环样品。另一个实施例中，毛细管在两个热交换器之间交替布置，使得样品仅穿过管中一次。其他实施例保持热交换器平稳，并在它们之间平移样品。另外的实施例保持样品静止，并且自动地将热交换器平移或旋转通过毛细管内包含的样品，以进行扩增反应所需的热循环。

2. 发明授权

US5720923A Nucleic acid amplification reaction apparatus 失效
公开(公告)号：US5720923A
公开(公告)日：1998-02-24
申请号：US299033
申请日：1994-08-31
申请人： Lawrence A. Haff , Enrico Picozza , Will Bloch , Robert Ragusa , Joseph DiCesare , David Tracy , Paul Saviano , Timothy M. Woudenberg
发明人： Lawrence A. Haff , Enrico Picozza , Will Bloch , Robert Ragusa , Joseph DiCesare , David Tracy , Paul Saviano , Timothy M. Woudenberg
IPC分类号： C12N15/09 , B01J19/00 , B01L7/00 , C12M1/00 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N35/00 , C12M1/40 , C12P19/34
CPC分类号： B01L7/525 , B01J19/0046 , B01L7/52 , B01L9/065 , B01J2219/00495 , B01J2219/00722 , B01L2300/1883 , C12Q1/686 , C40B40/06 , C40B60/14 , G01N2035/00237 , Y10T436/116664
摘要： Apparatus and method for performing a nucleic acid amplification reaction and preferably a polymerase chain reaction (PCR) in a reaction mixture in at least one capillary tube. Several different embodiments are disclosed. One embodiment cycles a sample through a capillary tube loop passing through two thermostatted fluid baths. Another embodiment has the capillary tube routed alternatingly between two heat exchangers to that the sample makes only one pass through the tube. Other embodiments maintain the heat exchangers stationary and translate the samples between them. Still further embodiments maintain the samples stationary and either automatically translate or rotate the heat exchangers past the samples contained within the capillary tubes to perform the thermal cycles necessary for the amplification reaction.

3. 发明授权

US5827480A Nucleic acid amplification reaction apparatus 失效
标题翻译：核酸扩增反应装置
公开(公告)号：US5827480A
公开(公告)日：1998-10-27
申请号：US823735
申请日：1997-03-25
申请人： Lawrence A. Haff , Enrico Picozza , Will Bloch , Timothy M. Woudenberg
发明人： Lawrence A. Haff , Enrico Picozza , Will Bloch , Timothy M. Woudenberg
IPC分类号： C12N15/09 , B01J19/00 , B01L7/00 , C12M1/00 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N35/00 , G01N21/01 , C12M1/02 , G01N21/62 , G01N33/50
CPC分类号： B01L7/525 , B01J19/0046 , B01L7/52 , B01L9/065 , B01J2219/00495 , B01J2219/00722 , B01L2300/1883 , C12Q1/686 , C40B40/06 , C40B60/14 , G01N2035/00237 , Y10T436/116664
摘要： Apparatus and method for performing a nucleic acid amplification reaction and preferably a polymerase chain reaction (PCR) in a reaction mixture in at least one capillary tube. Several different embodiments are disclosed. One embodiment cycles a sample through a capillary tube loop passing through two thermostatted fluid baths. Another embodiment has the capillary tube routed alternatingly between two heat exchangers to that the sample makes only one pass through the tube. Other embodiments maintain the heat exchangers stationary and translate the samples between them. Still further embodiments maintain the samples stationary and either automatically translate or rotate the heat exchangers past the samples contained within the capillary tubes to perform the thermal cycles necessary for the amplification reaction.
摘要翻译：用于在至少一个毛细管中的反应混合物中进行核酸扩增反应和优选聚合酶链式反应（PCR）的装置和方法。公开了几个不同的实施例。一个实施例通过穿过两个恒温流体浴的毛细管回路循环样品。另一个实施例中，毛细管在两个热交换器之间交替布置，使得样品仅穿过管中一次。其他实施例保持热交换器平稳，并在它们之间平移样品。另外的实施例保持样品静止，并且自动地将热交换器平移或旋转通过毛细管内包含的样品，以进行扩增反应所需的热循环。

4. 发明授权

US5779977A Nucleic acid amplification reaction apparatus and method 失效
公开(公告)号：US5779977A
公开(公告)日：1998-07-14
申请号：US827353
申请日：1997-03-26
申请人： Lawrence A. Haff , Enrico Picozza , Will Bloch , Timothy M. Woudenberg
发明人： Lawrence A. Haff , Enrico Picozza , Will Bloch , Timothy M. Woudenberg
IPC分类号： C12N15/09 , B01J19/00 , B01L7/00 , C12M1/00 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N35/00 , G01N15/06
CPC分类号： B01L7/525 , B01J19/0046 , B01L7/52 , B01L9/065 , B01J2219/00495 , B01J2219/00722 , B01L2300/1883 , C12Q1/686 , C40B40/06 , C40B60/14 , G01N2035/00237 , Y10T436/116664
摘要： Apparatus and method for performing a nucleic acid amplification reaction and preferably a polymerase chain reaction (PCR) in a reaction mixture in at least one capillary tube. Several different embodiments are disclosed. One embodiment cycles a sample through a capillary tube loop passing through two thermostatted fluid baths. Another embodiment has the capillary tube routed alternatingly between two heat exchangers to that the sample makes only one pass through the tube. Other embodiments maintain the heat exchangers stationary and translate the samples between them. Still further embodiments maintain the samples stationary and either automatically translate or rotate the heat exchangers past the samples contained within the capillary tubes to perform the thermal cycles necessary for the amplification reaction.

5. 发明授权

US08628925B2 Linear amplification of short nucleic acids 有权
标题翻译：短核酸的线性扩增
公开(公告)号：US08628925B2
公开(公告)日：2014-01-14
申请号：US13154128
申请日：2011-06-06
申请人： Will Bloch
发明人： Will Bloch
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q1/6853 , C12Q2531/101 , C12Q2525/301 , C12Q2521/107 , C12Q2527/125
摘要： The present teachings provide novel methods for amplifying short nucleic acids. In some embodiments, the present teachings provide novel methods for linearly amplifying a collection of micro RNAs by using temperature cycling during a reverse transcription reaction. The cycling can comprise at least 20 cycles of an annealing temperature segment of 10° C.-30° C., and a denaturation temperature segment of 35° C.-60° C. In some embodiments, the temperature cycled reaction can comprise an osmolyte.
摘要翻译：本教导提供了用于扩增短核酸的新方法。在一些实施方案中，本发明提供了用于在逆转录反应期间通过使用温度循环线性扩增微RNA集合的新方法。循环可以包括10℃-30℃的退火温度段和35℃-60℃的变性温度段的至少20个循环。在一些实施方案中，温度循环反应可以包括渗透剂

6. 发明申请

US20050037361A1 Methods for the reduction of stutter in microsatellite amplification 有权
公开(公告)号：US20050037361A1
公开(公告)日：2005-02-17
申请号：US10637466
申请日：2003-08-08
申请人： Sulekha Rao Coticone , Will Bloch
发明人： Sulekha Rao Coticone , Will Bloch
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566 , C12P19/34
CPC分类号： C12Q1/686 , C12Q1/6846 , C12Q1/6848 , C12Q1/6876 , C12Q2527/125 , C12Q2525/151
摘要： The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of 50% or less; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of betaine, sorbitol or mixtures thereof, effective to reduce stutter relative to the amount of stutter observed in the absence of betaine and/or sorbitol. The invention also provides compositions containing betaine and/or sorbitol, kits for amplifying microsatellites having a G+C content of 50% or less, and methods of using all of the foregoing.

7. 发明授权

US06841349B2 Methods for the reduction of stutter in microsatellite amplification 有权
标题翻译：减少微卫星放大的口吃的方法
公开(公告)号：US06841349B2
公开(公告)日：2005-01-11
申请号：US09850514
申请日：2001-05-07
申请人： Sulekha Rao Coticone , Will Bloch
发明人： Sulekha Rao Coticone , Will Bloch
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/686 , C12Q1/6846 , C12Q1/6848 , C12Q1/6876 , C12Q2527/125 , C12Q2525/151
摘要： The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of 50% or less; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of betaine, sorbitol or mixtures thereof, effective to reduce stutter relative to the amount of stutter observed in the absence of betaine and/or sorbitol. The invention also provides compositions containing betaine and/or sorbitol, kits for amplifying microsatellites having a G+C content of 50% or less, and methods of using all of the foregoing.
摘要翻译：本发明提供了减少微卫星扩增中的口吃的方法，包括以下步骤：提供包含G + C含量为50％或更少的微卫星的样品; 使样品与至少一种具有核酸聚合酶活性的酶接触; 并将样品与酶温育足够的时间和足以扩增微卫星的条件; 其中在一定量的甜菜碱，山梨糖醇或其混合物的存在下进行培养，其有效地相对于在不存在甜菜碱和/或山梨醇的情况下观察到的口吃量减少口吃。本发明还提供含有甜菜碱和/或山梨糖醇的组合物，用于扩增G + C含量为50％以下的微卫星的试剂盒，以及使用上述所有方法。

8. 发明授权

US5538871A In situ polymerase chain reaction 失效
标题翻译：原位聚合酶链反应
公开(公告)号：US5538871A
公开(公告)日：1996-07-23
申请号：US390256
申请日：1995-02-17
申请人： Gerard J. Nuovo , Will Bloch
发明人： Gerard J. Nuovo , Will Bloch
IPC分类号： A61B10/00 , B01L7/00 , C12M1/40 , C12N15/09 , C12Q1/68 , C12R1/19 , C12P19/34 , C07H21/04 , C12N15/00
CPC分类号： C12Q1/6841 , B01L7/52 , C12Q1/686
摘要： Improvements to the in situ polymerase chain reaction (PCR), a process of in vitro enzymatic amplification of specific nucleic acid sequences within the cells where they originate, can be achieved by changing the way that the enzymatic reaction is started. Reaction initiation is delayed until the start of PCR thermal cycling, either by withholding a subset of PCR reagents from the cellular preparation until the preparation has been heated to 50.degree. C. to 80.degree. C., immediately before thermal cycling is begun, or by adding to the PCR reagents a single-stranded DNA binding protein which blocks reaction at temperatures below about 50.degree. C. If the in situ PCR is performed on cellular preparations already attached to a microscope slide, thermal cycling also is facilitated by use of a thermal cycler sample block or compartment designed optimally to hold the microscope slide and any vapor barrier covering the slide.
摘要翻译：原位聚合酶链反应（PCR）的改进，其可以通过改变酶反应开始的方式来实现其在其中起源的细胞内的特定核酸序列的体外酶促扩增过程。反应开始被延迟直到PCR热循环的开始，通过从细胞制剂中扣留一组PCR试剂，直到制备在热循环开始之前立即加热至50℃至80℃，或通过向PCR试剂中加入在低于约50℃的温度下阻断反应的单链DNA结合蛋白。如果在已经连接到显微镜载玻片上的细胞制剂上进行原位PCR，则通过使用热循环仪样品块或隔室设计最佳，以保持显微镜载玻片和覆盖幻灯片的任何蒸汽屏障。

9. 发明申请

US20130011839A1 Methods for the Reduction of Stutter in Microsatellite Amplification 有权
公开(公告)号：US20130011839A1
公开(公告)日：2013-01-10
申请号：US13611974
申请日：2012-09-12
申请人： Sulekha Rao Coticone , Will Bloch
发明人： Sulekha Rao Coticone , Will Bloch
IPC分类号： C12Q1/68 , C12N9/12 , C12P19/34
CPC分类号： C12Q1/686 , C12Q1/6846 , C12Q1/6848 , C12Q1/6876 , C12Q2527/125 , C12Q2525/151
摘要： The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of 50% or less; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of betaine, sorbitol or mixtures thereof, effective to reduce stutter relative to the amount of stutter observed in the absence of betaine and/or sorbitol. The invention also provides compositions containing betaine and/or sorbitol, kits for amplifying microsatellites having a G+C content of 50% or less, and methods of using all of the foregoing.

10. 发明申请

US20110143352A1 Methods for the Reduction of Stutter in Microsatellite Amplification 审中-公开
标题翻译：减少微卫星放大技术的方法
公开(公告)号：US20110143352A1
公开(公告)日：2011-06-16
申请号：US12769577
申请日：2010-04-28
申请人： Sulekha Rao COTICONE , Will Bloch
发明人： Sulekha Rao COTICONE , Will Bloch
IPC分类号： C12Q1/68 , C12N9/12
CPC分类号： C12Q1/686 , C12Q1/6846 , C12Q1/6848 , C12Q1/6876 , C12Q2527/125 , C12Q2525/151
摘要： The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of 50% or less; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of betaine, sorbitol or mixtures thereof, effective to reduce stutter relative to the amount of stutter observed in the absence of betaine and/or sorbitol. The invention also provides compositions containing betaine and/or sorbitol, kits for amplifying microsatellites having a G+C content of 50% or less, and methods of using all of the foregoing.
摘要翻译：本发明提供了减少微卫星扩增中的口吃的方法，包括以下步骤：提供包含G + C含量为50％或更少的微卫星的样品; 使样品与至少一种具有核酸聚合酶活性的酶接触; 并将样品与酶温育足够的时间和足以扩增微卫星的条件; 其中在一定量的甜菜碱，山梨糖醇或其混合物的存在下进行培养，其有效地相对于在不存在甜菜碱和/或山梨醇的情况下观察到的口吃量减少口吃。本发明还提供含有甜菜碱和/或山梨糖醇的组合物，用于扩增G + C含量为50％以下的微卫星的试剂盒，以及使用上述所有方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式