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1. 发明申请

US20110230386A1 Protease Variants and Compositions 审中-公开
标题翻译：蛋白酶变体和组成
公开(公告)号：US20110230386A1
公开(公告)日：2011-09-22
申请号：US13115653
申请日：2011-05-25
申请人： Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus Von der Osten , Peter Bauditz
发明人： Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus Von der Osten , Peter Bauditz
IPC分类号： C11D3/386 , C12N9/50 , C12N9/54
CPC分类号： C12N9/54 , C11D3/386
摘要： The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.
摘要翻译：本发明涉及通过突变多个枯草芽孢杆菌基因并在合适的宿主中表达突变的基因而产生的酶。与其野生型亲本酶相比，酶在任何洗涤剂中表现出改进的稳定性和/或改善的洗涤性能。这些酶非常适合用于任何洗涤剂和一些特别是液体或固体形式的洗涤剂组合物中。

2. 发明申请

US20090163400A1 Subtilase Variants 审中-公开
标题翻译：脱铁酶变体
公开(公告)号：US20090163400A1
公开(公告)日：2009-06-25
申请号：US12185994
申请日：2008-08-05
申请人： Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus Von der Osten , Peter Bauditz
发明人： Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus Von der Osten , Peter Bauditz
IPC分类号： C11D3/386 , C12N9/54 , C12N9/56 , C12S11/00
CPC分类号： C12N9/54 , C11D3/386
摘要： The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.
摘要翻译：本发明涉及通过突变多个枯草芽孢杆菌基因并在合适的宿主中表达突变的基因而产生的酶。与其野生型亲本酶相比，酶在任何洗涤剂中表现出改进的稳定性和/或改善的洗涤性能。这些酶非常适合用于任何洗涤剂和一些特别是液体或固体形式的洗涤剂组合物中。

3. 发明授权

US07993898B2 Cellulase variants 有权
公开(公告)号：US07993898B2
公开(公告)日：2011-08-09
申请号：US12394202
申请日：2009-02-27
申请人： Kim Vilbour Andersen , Martin Schulein , Torben Henriksen, legal representative , Lars Christensen , Bo Damgaard , Claus Von der Osten
发明人： Kim Vilbour Andersen , Martin Schulein , Lars Christensen , Bo Damgaard , Claus Von der Osten
IPC分类号： C12N9/42 , C12N1/20 , C12N15/00 , C12Q1/00 , C12Q1/68 , C12P21/04 , C07H21/04
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

4. 发明申请

US20120289450A1 Cellulase Variants 审中-公开
标题翻译：纤维素酶变体
公开(公告)号：US20120289450A1
公开(公告)日：2012-11-15
申请号：US13471757
申请日：2012-05-15
申请人： Kim Vilbour Andersen , Martin Schülein , Lars Christiansen , Bo Damgaard , Claus Von der Osten
发明人： Kim Vilbour Andersen , Martin Schülein , Lars Christiansen , Bo Damgaard , Claus Von der Osten
IPC分类号： C12N9/42 , C11D3/386
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to cellulase variants, i.e., endo-beta-1,4-glucanase variants, derived from a parental cellulase, i.e., endo-beta-1,4-glucanase, by substitution, insertion and/or deletion, which variant has a catalytic core domain, in which the variant at position 5 holds an alanine residue (A), a serine residue (S), or a threonine residue (T); at position 8 holds a phenylalanine residue (F), or a tyrosine residue (Y); at position 9 holds a phenylalanine residue (F), a tryptophan residue (W), or a tyrosine residue (Y); at position 10 holds an aspartic acid residue (D); and at position 121 holds an aspartic acid residue (D).
摘要翻译：本发明涉及通过取代，插入和/或缺失衍生自亲代纤维素酶（即内切-β-1,4-葡聚糖酶）的纤维素酶变体，即内切-β-1,4-葡聚糖酶变体，该变体具有催化核心结构域，其中第5位的变体含有丙氨酸残基（A），丝氨酸残基（S）或苏氨酸残基（T）; 在8位保持苯丙氨酸残基（F）或酪氨酸残基（Y）; 位置9含有苯丙氨酸残基（F），色氨酸残基（W）或酪氨酸残基（Y）; 在10位保持天冬氨酸残基（D）; 并且在位置121处保持天冬氨酸残基（D）。

5. 发明申请

US20110136206A1 Alpha-amylase variant with altered properties 有权
标题翻译：具有改变性质的α-淀粉酶变体
公开(公告)号：US20110136206A1
公开(公告)日：2011-06-09
申请号：US13020545
申请日：2011-02-03
申请人： Allan Svendsen , Carsten Andersen , Thomas Thisted , Claus Von der Osten
发明人： Allan Svendsen , Carsten Andersen , Thomas Thisted , Claus Von der Osten
IPC分类号： C12N9/26 , C07H21/04 , C12N15/63 , C12N1/21 , C12N1/15
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
摘要翻译：本发明涉及亲本Termamyl样α-淀粉酶的变体（突变体），该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质。

6. 发明申请

US20110250674A1 CELLULASE VARIANTS 审中-公开
标题翻译：纤维素酶变体
公开(公告)号：US20110250674A1
公开(公告)日：2011-10-13
申请号：US13162636
申请日：2011-06-17
申请人： Kim Vilbour Andersen , Martin Schülein , Torben Henriksen , Lars Christiansen , Bo Damgaard , Claus Von der Osten
发明人： Kim Vilbour Andersen , Martin Schülein , Torben Henriksen , Lars Christiansen , Bo Damgaard , Claus Von der Osten
IPC分类号： C12N9/42
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译：本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。鉴定酶的表面暴露的氨基酸残基; e。鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。通过使用常规蛋白质工程技术进行取代，缺失或插入。还描述了通过该方法获得的纤维素酶变体。

7. 发明申请

US20050009166A1 Cellulase variants 审中-公开
标题翻译：纤维素酶变体
公开(公告)号：US20050009166A1
公开(公告)日：2005-01-13
申请号：US10919195
申请日：2004-08-16
申请人： Kim Andersen , Martin Schulein , Hanne Dela , Lars Christiansen , Bo Damgaard , Claus Von der Osten
发明人： Kim Andersen , Martin Schulein , Hanne Dela , Lars Christiansen , Bo Damgaard , Claus Von der Osten
IPC分类号： C12N15/09 , C11D3/386 , C12N1/15 , C12N1/21 , C12N9/42 , C12R1/645 , C12S11/00 , C12S99/00 , D06L4/40 , D06M16/00 , D21B1/02 , D21C5/02 , D21H21/24 , C07H21/04 , C12N15/74
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译：本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。鉴定酶的表面暴露的氨基酸残基; e。鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。通过使用常规蛋白质工程技术进行取代，缺失或插入。还描述了通过该方法获得的纤维素酶变体。

8. 发明授权

US08017372B2 Cellulase variants 有权
标题翻译：纤维素酶变体
公开(公告)号：US08017372B2
公开(公告)日：2011-09-13
申请号：US11830063
申请日：2007-07-30
申请人： Kim Vilbour Andersen , Martin Schulein , Torben Henriksen, legal representative , Lars Christiansen , Bo Damgaard , Claus Von der Osten
发明人： Kim Vilbour Andersen , Martin Schulein , Lars Christiansen , Bo Damgaard , Claus Von der Osten
IPC分类号： C12N9/42 , C12N15/00 , C12P21/04 , C07H21/04
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译：本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。鉴定酶的表面暴露的氨基酸残基; e。鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。通过使用常规蛋白质工程技术进行取代，缺失或插入。还描述了通过该方法获得的纤维素酶变体。

9. 发明申请

US20090170747A1 CELLULASE VARIANTS 有权
标题翻译：纤维素酶变体
公开(公告)号：US20090170747A1
公开(公告)日：2009-07-02
申请号：US12394202
申请日：2009-02-27
申请人： Kim Vilbour Andersen , Martin Schulein , Torben Henriksen , Lars Christensen , Bo Damgaard , Claus Von der Osten
发明人： Kim Vilbour Andersen , Martin Schulein , Torben Henriksen , Lars Christensen , Bo Damgaard , Claus Von der Osten
IPC分类号： C11D3/386 , C12N9/42
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of:a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译：本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。鉴定酶的表面暴露的氨基酸残基; e。鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。通过使用常规蛋白质工程技术进行取代，缺失或插入。还描述了通过该方法获得的纤维素酶变体。

10. 发明申请

US20080206836A1 Cellulase Variants 有权
标题翻译：纤维素酶变体
公开(公告)号：US20080206836A1
公开(公告)日：2008-08-28
申请号：US11830063
申请日：2007-07-30
申请人： Kim Vilbour Andersen , Martin Schulein , Torben Henriksen , Lars Christiansen , Bo Damgaard , Claus Von der Osten
发明人： Kim Vilbour Andersen , Martin Schulein , Torben Henriksen , Lars Christiansen , Bo Damgaard , Claus Von der Osten
IPC分类号： C12N9/42
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译：本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。鉴定酶的表面暴露的氨基酸残基; e。鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。通过使用常规蛋白质工程技术进行取代，缺失或插入。还描述了通过该方法获得的纤维素酶变体。

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