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1. 发明授权

US07189519B2 Bead-based detection of ligand-GPCR-G protein complexes 有权
标题翻译：基于珠粒的配体-GPCR-G蛋白复合物的检测
公开(公告)号：US07189519B2
公开(公告)日：2007-03-13
申请号：US10429042
申请日：2003-05-05
申请人： Larry A. Sklar , Tione Buranda , Daniel Cimino , Alex T. Key , Richard Neubig , Peter C. Simons , Eric R. Prossnitz , Mei Shi
发明人： Larry A. Sklar , Tione Buranda , Daniel Cimino , Alex T. Key , Richard Neubig , Peter C. Simons , Eric R. Prossnitz , Mei Shi
IPC分类号： G01N33/52 , G01N33/566 , C12N1/20 , C07K1/00 , C07H21/04
CPC分类号： C07D401/12 , B01F13/0059 , B01F13/0093 , B01F13/0096 , B01F15/0201 , B01F15/024 , C07D233/76 , C07D405/12 , C40B40/00 , G01N33/542 , G01N33/566 , G01N2333/726 , Y10T436/13
摘要： The present invention provides a general approach for G protein coupled receptors that may be used to define agonists and antagonists, and the specificity of receptor coupling to G protein subunits. Methods of the present invention use small volumes (microliters) and are compatible with high throughput flow cytometry. When assays of the present invention are multiplexed, the specificity of the interactions of a receptor with many G proteins may be determined simultaneously.
摘要翻译：本发明提供了可用于定义激动剂和拮抗剂的G蛋白偶联受体的一般方法以及受体与G蛋白亚基偶联的特异性。本发明的方法使用小体积（微升）并且与高通量流式细胞术兼容。当本发明的测定被复用时，可以同时测定受体与许多G蛋白的相互作用的特异性。

2. 发明授权

US07785900B1 Glutathione beads and GST fusion proteins 有权
标题翻译：谷胱甘肽和GST融合蛋白
公开(公告)号：US07785900B1
公开(公告)日：2010-08-31
申请号：US11652432
申请日：2007-01-11
申请人： Peter C. Simons , Larry A. Sklar , Eric R. Prossnitz , Angela Wandinger-Ness , Mathewos Z. Tessema , John C. Reed , Dayong Zhai
发明人： Peter C. Simons , Larry A. Sklar , Eric R. Prossnitz , Angela Wandinger-Ness , Mathewos Z. Tessema , John C. Reed , Dayong Zhai
IPC分类号： G01N33/543 , G01N33/53
CPC分类号： G01N33/54393 , G01N33/543
摘要： The present invention relates generally to glutathione derivatized beads which are adapted for use in conjunction with glutathione-S-transferase fusion proteins (generally, GST fusion proteins, which contain a fluorescent label such as fluorescent green protein) for use in flow cytometry. The present invention also relates to methods for detecting and/or quantifying interactions between a GST fusion protein and their binding partners, in particular, labeled binding partners such as fluorescently labeled binding partners. By creating glutathione beads with an appropriate high or increased site density, disadvantages often associated with low affinity systems and quick off-rates in solution may be resolved to provide a workable system and method. Methods of identifying potential agonists, antagonists and regulator compounds of proteins fused to GST from libraries of compounds represents another aspect of the present invention.
摘要翻译：本发明一般涉及适用于与流式细胞术中使用的谷胱甘肽-S-转移酶融合蛋白（通常为含有荧光标记如荧光绿蛋白的GST融合蛋白）结合使用的谷胱甘肽衍生化珠粒。本发明还涉及用于检测和/或定量GST融合蛋白与其结合配偶体，特别是标记的结合配偶体如荧光标记的结合配偶体之间的相互作用的方法。通过产生具有适当的高或增加的位点密度的谷胱甘肽珠，可以解决通常与低亲和力系统相关的缺点和解决方案中的快速关闭速率，以提供可行的系统和方法。从化合物文库鉴定与GST融合的蛋白质的潜在激动剂，拮抗剂和调节剂化合物的方法代表本发明的另一方面。

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