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    • 5. 发明申请
    • SURFACE ATTACHMENT
    • 表面贴附
    • WO2009112498A1
    • 2009-09-17
    • PCT/EP2009052801
    • 2009-03-10
    • ITI SCOTLAND LTDCAMPBELL COLINSCHULZE HOLGERBACHMANN TILL
    • CAMPBELL COLINSCHULZE HOLGERBACHMANN TILL
    • C12Q1/68G01N33/543
    • C12Q1/6837B01J2219/00605B01J2219/00626B01J2219/00637B01J2219/00659B01J2219/00722B01J2219/00725C40B50/18C40B70/00G01N33/54353
    • Provided is a method of attaching a substance to a surface, which method comprises contacting a surface comprising amine reactive groups with a substance labelled with a peptide tag such that the substance is covalently attached to the surface via the peptide tag, wherein the peptide tag comprises one or more histidine residues, one of which is a terminal histidine residue having a free N-terminal amino group. Also provided is a method of processing or analysis which comprises a method of attaching a substance to a surface as detailed above and comprises one or more further steps of processing or analysing the substance. The present invention farther provides a method of processing or analysis comprising the steps of: a) attaching a substance to a surface by a method as defined above b) contacting the surface with a sample comprising an analyte in order that the analyte in the sample binds to the substance attached to the surface; and c) processing and/or analysing the analyte
    • 提供了将物质附着到表面的方法,该方法包括使包含胺反应性基团的表面与标记有肽标签的物质接触,使得物质通过肽标签共价连接到表面,其中肽标签包含 一个或多个组氨酸残基,其中之一是具有游离N-末端氨基的末端组氨酸残基。 还提供了一种处理或分析方法,其包括将物质附着到如上所述的表面的方法,并且包括处理或分析物质的一个或多个其它步骤。 本发明还提供了一种处理或分析方法,其包括以下步骤:a)通过上述方法将物质附着于表面b)使表面与包含分析物的样品接触,以使样品中的分析物结合 附着于表面的物质; 和c)处理和/或分析分析物