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    • 5. 发明申请
    • METHODS AND SYSTEMS FOR IDENTIFICATION OF MACROMOLECULES
    • 用于鉴定大分子的方法和系统
    • WO2005088302A1
    • 2005-09-22
    • PCT/US2005/005524
    • 2005-02-22
    • PROTEOGENIX, INC.NAGALLA, SrinivasaSEARLE, BrianDASARI, SurendraTURNER, Mark, A.
    • NAGALLA, SrinivasaSEARLE, BrianDASARI, SurendraTURNER, Mark, A.
    • G01N33/48
    • G06F19/22G06F19/28H01J49/0036
    • A method is provided for identifying sequences of molecules and sequence modifications form mass spectrometry data. At least one de novo sequence is produced from mass spectrometry data of sequences of molecules. A least one mass-based alignment is calculated between each de novo sequence and sequences in a sequence database. The molecular masses of molecules in the de novo sequence are compared to molecular masses of molecules in each sequence in the sequence database. Mass differences of modification sites are interpreted between the sequence in the sequence database and the novo sequence that have been identified by the mass-based alignment as modifications identified in a modification catalog. At least one match score for the mass-based alignment is calculated that provides an indication of matching between the sequence in the sequence database and the novo sequence. Sequences in the sequence database are identified from mass-based alignments in response to the match scores. Identifications of the sequence database are grouped from at least one de novo sequence into an identified macromolecule list that agrees with the novo sequencing results.
    • 提供了用于鉴定分子序列和形成质谱数据的序列修饰的方法。 由分子序列的质谱数据产生至少一个从头序列。 在序列数据库中的每个从头序列和序列之间计算至少一个基于质量的比对。 将从头序列中的分子的分子质量与序列数据库中的每个序列中的分子的分子量进行比较。 修饰位点的质量差异在序列数据库中的序列和通过基于质量的校准被鉴定为在修改目录中确定的修饰的新离子序列之间进行了解释。 计算用于基于质量的比对的至少一个匹配得分,其提供序列数据库中的序列和新诗序列之间的匹配的指示。 序列数据库中的序列根据匹配分数从基于质量的比对中确定。 序列数据库的识别从至少一个从头序列分组到与新呼号测序结果一致的识别的大分子列表中。